##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140123_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5787683
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49233708895252	33.0	32.0	33.0	27.0	34.0
2	32.3683688273874	33.0	33.0	34.0	31.0	34.0
3	32.56554963359258	33.0	33.0	34.0	31.0	34.0
4	32.951844460036945	33.0	33.0	34.0	31.0	34.0
5	33.102463110021745	34.0	33.0	34.0	33.0	34.0
6	36.55076167785969	38.0	37.0	38.0	34.0	38.0
7	37.114459620542455	38.0	38.0	38.0	36.0	38.0
8	37.05411474678209	38.0	38.0	38.0	36.0	38.0
9	37.22051605106914	38.0	38.0	38.0	36.0	38.0
10-11	37.331234364425285	38.0	38.0	38.0	37.0	38.0
12-13	37.36858592635429	38.0	38.0	38.0	37.0	38.0
14-15	37.393239142503134	38.0	38.0	38.0	37.0	38.0
16-17	37.401215996107595	38.0	38.0	38.0	37.0	38.0
18-19	37.4201264996718	38.0	38.0	38.0	37.0	38.0
20-21	37.43132509982803	38.0	38.0	38.0	37.0	38.0
22-23	37.44862152247846	38.0	38.0	38.0	37.0	38.0
24-25	37.45709813520088	38.0	38.0	38.0	37.0	38.0
26-27	37.428044138674935	38.0	38.0	38.0	37.0	38.0
28-29	37.409678271073105	38.0	38.0	38.0	37.0	38.0
30-31	37.40316004282349	38.0	38.0	38.0	37.0	38.0
32-33	37.39516991207802	38.0	38.0	38.0	37.0	38.0
34-35	37.36874187161516	38.0	38.0	38.0	37.0	38.0
36-37	37.344309844700646	38.0	38.0	38.0	37.0	38.0
38-39	37.3129423500135	38.0	38.0	38.0	37.0	38.0
40-41	37.32980017015305	38.0	38.0	38.0	37.0	38.0
42-43	37.293851966867635	38.0	38.0	38.0	37.0	38.0
44-45	37.247586696438034	38.0	38.0	38.0	37.0	38.0
46-47	37.21070490500972	38.0	38.0	38.0	37.0	38.0
48-49	37.16384586848493	38.0	38.0	38.0	37.0	38.0
50-51	37.12807483643574	38.0	38.0	38.0	36.0	38.0
52-53	37.08896342685334	38.0	38.0	38.0	36.0	38.0
54-55	37.06837182247054	38.0	38.0	38.0	36.0	38.0
56-57	37.02168951954161	38.0	38.0	38.0	36.0	38.0
58-59	36.98870972742138	38.0	38.0	38.0	36.0	38.0
60-61	37.00417911576828	38.0	38.0	38.0	36.0	38.0
62-63	37.015316767069834	38.0	38.0	38.0	36.0	38.0
64-65	37.02278586111156	38.0	38.0	38.0	36.0	38.0
66-67	37.0091766917791	38.0	38.0	38.0	36.0	38.0
68-69	36.96621034169463	38.0	38.0	38.0	36.0	38.0
70-71	36.95937672192437	38.0	38.0	38.0	36.0	38.0
72-73	36.9686059211207	38.0	38.0	38.0	36.0	38.0
74-75	36.98251776528282	38.0	38.0	38.0	36.0	38.0
76-77	36.95986209269611	38.0	38.0	38.0	36.0	38.0
78-79	36.9162659649885	38.0	38.0	38.0	35.0	38.0
80-81	36.8648104980046	38.0	38.0	38.0	35.0	38.0
82-83	36.81909199283659	38.0	38.0	38.0	35.0	38.0
84-85	36.78513856757253	38.0	38.0	38.0	35.0	38.0
86-87	36.805576219443424	38.0	38.0	38.0	35.0	38.0
88-89	36.760014966528956	38.0	38.0	38.0	35.0	38.0
90-91	36.77039771499584	38.0	38.0	38.0	35.0	38.0
92-93	36.754555803930685	38.0	38.0	38.0	35.0	38.0
94-95	36.76616467476137	38.0	38.0	38.0	35.0	38.0
96-97	36.76243732864359	38.0	38.0	38.0	35.0	38.0
98-99	36.742550745059816	38.0	38.0	38.0	34.0	38.0
100	35.20205936000011	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	10.0
19	13.0
20	16.0
21	81.0
22	268.0
23	931.0
24	2272.0
25	4910.0
26	8655.0
27	14649.0
28	22930.0
29	33724.0
30	46307.0
31	60830.0
32	79397.0
33	105608.0
34	157129.0
35	275372.0
36	726886.0
37	4247694.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.738153247162984	19.181959343661358	13.920561993460941	25.15932541571472
2	14.659167470320671	19.535557391436726	42.64498906103203	23.160286077210575
3	18.55594934329291	25.157593806413086	29.648663087560024	26.63779376273398
4	12.917605314274597	17.331106496633655	37.35606683412866	32.39522135496309
5	14.064229156987349	36.595024295560066	34.90210158365619	14.438644963796394
6	31.145900699813726	36.35829743957988	18.00812173023298	14.487680130373414
7	27.392516141606237	32.00016310499383	21.87509232969394	18.732228423705997
8	24.926969911793716	36.27743606552052	20.39209818505955	18.403495837626213
9	25.66294664030494	16.928294103184296	20.475240264541096	36.93351899196967
10-11	25.007788090674627	26.113619215150518	27.832208502089696	21.046384192085156
12-13	25.81730893001569	23.42137604979402	28.175575614628514	22.585739405561778
14-15	22.92214345533437	24.683150407511953	25.691196287011575	26.703509850142105
16-17	22.640303554980466	28.096105125315262	26.27541107555476	22.988180244149515
18-19	22.70824957068312	26.866018750508623	28.235461410032304	22.19027026877595
20-21	23.329252207039232	25.99710278045303	28.12651076833336	22.547134244174373
22-23	23.14647641964308	26.14094390543079	28.078455644247878	22.634124030678247
24-25	23.083754663227253	26.288738979024313	28.101628243695863	22.52587811405257
26-27	22.968753753502043	26.332392772329914	28.171533163978204	22.52732031018984
28-29	22.75889981065557	26.159492601183093	28.250847831529097	22.83075975663224
30-31	22.91069620226329	26.147365375158536	28.29968759683369	22.642250825744487
32-33	22.404647986558995	26.10106335699962	28.594630101349523	22.899658555091865
34-35	22.579007157194386	26.126250596143034	28.368165766238896	22.926576480423684
36-37	22.696539773447018	26.218802869325426	28.446068410086166	22.638588947141393
38-39	22.621025409587098	26.140713750788553	28.406330639614826	22.83193020000952
40-41	22.80356566456046	25.984647833142464	28.421848538846906	22.789937963450168
42-43	22.93295927733949	26.06799604338741	28.267530758567393	22.73151392070571
44-45	22.732280441077247	26.334857570413895	28.116426502934345	22.81643548557452
46-47	23.13880366021567	26.121361947707555	27.616299894320367	23.123534497756403
48-49	22.969765970488236	26.212818947723644	27.559693786054968	23.257721295733152
50-51	22.69179397644158	26.444986420731176	27.748977924572156	23.11424167825509
52-53	22.954246031233446	26.41967348822401	27.806823948731868	22.819256531810677
54-55	22.86759490206433	26.378417950958966	27.54752772112812	23.20645942584859
56-57	23.101235891217534	26.646879355069203	27.276461316615286	22.975423437097977
58-59	22.725237316075333	26.9584826643504	27.655441779088573	22.660838240485692
60-61	22.974909405037543	27.22173428997331	27.164429364009564	22.63892694097958
62-63	22.680776239972182	27.572742421702305	27.0873207876959	22.659160550629608
64-65	22.658474789690516	27.51899433704584	27.065486058808712	22.757044814454925
66-67	22.744816975787156	27.358914754148973	27.031570543711403	22.86469772635247
68-69	22.69791477767879	27.155694190089953	27.27433305939984	22.87205797283142
70-71	22.81766406850038	26.799173688180282	27.318965856516463	23.06419638680287
72-73	22.998562427293816	26.556236191221842	27.363575542677527	23.081625838806815
74-75	22.833677606804883	26.662006223290604	27.384752128291055	23.119564041613454
76-77	23.07579093487573	26.59358689751043	27.23696854710219	23.09365362051165
78-79	22.99685363387761	26.6251556583388	27.184747320145586	23.193243387638006
80-81	23.01518541489516	26.681068828025456	27.217045423532177	23.08670033354721
82-83	23.074095901083567	26.64413071013472	27.26785463702154	23.01391875176017
84-85	23.165116036903047	26.650970790350787	27.154059065286418	23.02985410745975
86-87	23.028401540018546	26.64284607715011	27.26898066679113	23.05977171604021
88-89	23.04684459442129	26.62792966898657	27.136755615675156	23.188470120916975
90-91	23.12786093333681	26.640185747599922	27.16000431687923	23.071949002184038
92-93	23.14266235331766	26.714986181119144	27.09214571626098	23.050205749302215
94-95	23.01008226217758	26.73724973165345	27.166454235260783	23.086213770908188
96-97	23.174818163428643	26.75140353500262	27.146404458477353	22.927373843091384
98-99	24.283473880728362	28.748398961865206	27.286734858172707	19.681392299233725
100	31.271343616984755	39.9750834387046	28.753572944310648	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1606.0
1	1428.0
2	1657.5
3	2168.0
4	2673.5
5	3798.0
6	4914.5
7	6375.5
8	8174.0
9	8984.0
10	8401.0
11	7683.5
12	8231.0
13	10232.5
14	14306.0
15	19136.0
16	22734.5
17	24631.0
18	24308.0
19	22026.0
20	19205.5
21	16919.5
22	16452.5
23	18508.5
24	22638.0
25	28623.5
26	36402.0
27	45289.5
28	54256.5
29	64540.0
30	75511.0
31	86603.5
32	98042.5
33	109706.0
34	120442.0
35	130970.5
36	142788.0
37	154392.0
38	165931.5
39	175450.5
40	184983.0
41	195582.0
42	203910.0
43	212283.0
44	228928.5
45	251247.0
46	258526.0
47	257530.5
48	257929.0
49	254219.5
50	251532.5
51	249464.0
52	245722.5
53	239913.0
54	231404.5
55	219998.5
56	205740.0
57	188612.0
58	170108.0
59	147326.5
60	122713.5
61	101029.0
62	81283.0
63	63357.5
64	47951.5
65	35797.0
66	26390.5
67	19989.0
68	15146.0
69	10909.0
70	7812.0
71	5605.0
72	3985.5
73	2704.0
74	1853.0
75	1284.5
76	847.0
77	529.5
78	349.0
79	224.5
80	137.5
81	90.5
82	57.5
83	36.5
84	25.5
85	16.0
86	9.5
87	5.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.009917612972237769
3	3.2828335622389827E-4
4	0.0017450852094007222
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	8.639423052417021E-6
24-25	5.184000928972966E-5
26-27	6.740056084179499E-4
28-29	0.0014522188867290016
30-31	8.220385240133894E-4
32-33	8.844970814631351E-4
34-35	0.0012172690481955942
36-37	0.0
38-39	2.1959136155691328E-4
40-41	8.047442058638261E-4
42-43	3.561294779943144E-4
44-45	0.0
46-47	0.0
48-49	0.001709202814097322
50-51	0.003816819580690101
52-53	0.0
54-55	0.0039431792552430235
56-57	0.03919767367946107
58-59	0.055616335137918416
60-61	0.02983120326556981
62-63	0.007023635503584965
64-65	0.0
66-67	0.0
68-69	3.004021784364908E-5
70-71	0.003387739345306186
72-73	0.007719778019890301
74-75	0.005128024132855271
76-77	0.007085868461753343
78-79	0.010525255796011648
80-81	0.011118787604831225
82-83	0.04223166615765162
84-85	0.025682133187914125
86-87	0.0011365077060187792
88-89	0.0014608403891366555
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	37.0
20-21	153.0
22-23	359.0
24-25	601.0
26-27	1494.0
28-29	3977.0
30-31	11866.0
32-33	14290.0
34-35	20049.0
36-37	32641.0
38-39	39049.0
40-41	37495.0
42-43	41717.0
44-45	49953.0
46-47	51360.0
48-49	48124.0
50-51	43834.0
52-53	41588.0
54-55	43931.0
56-57	46084.0
58-59	46166.0
60-61	47263.0
62-63	46906.0
64-65	52109.0
66-67	58340.0
68-69	59588.0
70-71	71840.0
72-73	48873.0
74-75	46234.0
76-77	47391.0
78-79	49480.0
80-81	49755.0
82-83	46701.0
84-85	45446.0
86-87	47176.0
88-89	49419.0
90-91	49932.0
92-93	49382.0
94-95	57508.0
96-97	183417.0
98-99	1368217.0
100-101	2737938.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.54602280561005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.52798279770118	73.96160869751398
2	7.89464359346192	13.191321474030657
3	1.9934143516711282	4.996255226572393
4	0.7570835818035513	2.5300528876444974
5	0.3189022525713775	1.3321507433044362
6	0.1774038482956023	0.8892831573304425
7	0.10559957055883357	0.6175696891119655
8	0.06362830233845057	0.42527132786003546
9	0.03686733994269272	0.2772107661270946
>10	0.12187090772598999	1.5372884893010443
>50	0.0018669657752347505	0.10310422885962309
>100	6.330953609804698E-4	0.08469390372668263
>500	1.0339279303465132E-4	0.0541894086171777
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.727807138020517E-5
2	0.0	3.455614276041034E-5	0.0	0.0	1.727807138020517E-5
3	0.0	3.455614276041034E-5	0.0	0.0	1.727807138020517E-5
4	0.0	6.911228552082068E-5	0.0	1.727807138020517E-5	3.455614276041034E-5
5	0.0	6.911228552082068E-5	0.0	3.455614276041034E-5	3.455614276041034E-5
6	0.0	8.639035690102585E-5	0.0	3.455614276041034E-5	3.455614276041034E-5
7	0.0	8.639035690102585E-5	0.0	3.455614276041034E-5	3.455614276041034E-5
8	0.0	1.0366842828123102E-4	0.0	3.455614276041034E-5	5.183421414061551E-5
9	0.0	1.5550264242184653E-4	0.0	3.455614276041034E-5	5.183421414061551E-5
10-11	3.455614276041034E-5	1.5550264242184653E-4	0.0	3.455614276041034E-5	6.911228552082068E-5
12-13	5.183421414061551E-5	1.5550264242184653E-4	0.0	5.183421414061551E-5	6.911228552082068E-5
14-15	6.911228552082069E-5	3.54200463294206E-4	0.0	5.183421414061551E-5	1.5550264242184653E-4
16-17	9.502939259112844E-5	3.6283949898430857E-4	0.0	7.775132121092327E-5	1.727807138020517E-4
18-19	1.0366842828123102E-4	3.9739564174471893E-4	0.0	8.639035690102585E-5	1.9005878518225686E-4
20-21	1.0366842828123102E-4	4.3195178450512923E-4	0.0	1.0366842828123102E-4	2.0733685656246204E-4
22-23	1.2094649966143619E-4	4.665079272655396E-4	0.0	1.2094649966143619E-4	2.4189299932287237E-4
24-25	1.7278071380205168E-4	5.0106407002595E-4	0.0	1.5550264242184653E-4	2.937272134634879E-4
26-27	2.0733685656246204E-4	5.183421414061551E-4	0.0	1.8141974949215429E-4	3.3692239191400084E-4
28-29	2.0733685656246204E-4	5.269811770962577E-4	0.0	3.887566060546163E-4	3.455614276041034E-4
30-31	2.2461492794266722E-4	5.528982841665654E-4	0.0	0.0011835478895440542	3.54200463294206E-4
32-33	2.4189299932287237E-4	5.788153912368733E-4	0.0	0.002790408527903135	3.6283949898430857E-4
34-35	2.50532035012975E-4	7.08400926588412E-4	0.0	0.004915611307668371	3.9739564174471893E-4
36-37	2.5917107070307755E-4	8.293474262498482E-4	0.0	0.008241640048357866	4.146737131249241E-4
38-39	2.5917107070307755E-4	8.552645333201559E-4	0.0	0.013330032069828288	4.3195178450512923E-4
40-41	2.5917107070307755E-4	0.0010021281400519	0.0	0.019221854410478252	4.3195178450512923E-4
42-43	2.5917107070307755E-4	0.0010453233185024127	0.0	0.02486314471611524	4.492298558853344E-4
44-45	2.678101063931801E-4	0.0011576307824737464	0.0	0.031472007019043714	4.665079272655396E-4
46-47	2.764491420832827E-4	0.001226743067994567	0.0	0.03768347368022748	5.0106407002595E-4
48-49	2.764491420832827E-4	0.001226743067994567	0.0	0.043929496484171646	5.701763555467707E-4
50-51	2.764491420832827E-4	0.0013304114962757981	0.0	0.050693861429521966	5.788153912368733E-4
52-53	2.764491420832827E-4	0.0013390505319659006	0.0	0.05741503119642179	6.220105696873861E-4
54-55	2.764491420832827E-4	0.001356328603346106	0.0	0.0638424737498581	6.652057481378991E-4
56-57	2.764491420832827E-4	0.0013649676390362084	0.0	0.07033038955312515	6.738447838280017E-4
58-59	2.764491420832827E-4	0.0013908847461065162	0.0	0.0765245781429287	6.911228552082068E-4
60-61	2.764491420832827E-4	0.0014081628174867212	0.0	0.08263237637583122	7.343180336587198E-4
62-63	2.764491420832827E-4	0.001434079924557029	0.0	0.08880928689425457	7.602351407290274E-4
64-65	2.764491420832827E-4	0.0015895825669788757	0.0	0.09432099166454003	7.602351407290274E-4
66-67	2.764491420832827E-4	0.0015895825669788757	0.0	0.10048062411158316	7.688741764191301E-4
68-69	3.023662491535905E-4	0.0015895825669788757	0.0	0.10644155873775395	7.947912834894379E-4
70-71	3.1100528484369306E-4	0.0016154996740491835	0.0	0.1115731459376749	8.379864619399507E-4
72-73	3.1100528484369306E-4	0.0016500558168095936	0.0	0.1156853269261637	8.466254976300533E-4
74-75	3.1100528484369306E-4	0.0016759729238799014	0.0	0.11844117931130643	8.466254976300533E-4
76-77	3.1100528484369306E-4	0.0017450852094007222	0.0	0.12117111458937886	8.466254976300533E-4
78-79	3.1100528484369306E-4	0.0017796413521611325	0.0	0.12294211690584989	8.466254976300533E-4
80-81	3.1100528484369306E-4	0.0017796413521611325	0.0	0.12371099108226902	8.811816403904637E-4
82-83	3.1964432053379564E-4	0.0017796413521611325	0.0	0.12417749900953456	9.502939259112843E-4
84-85	3.282833562238982E-4	0.0017969194235413378	0.0	0.12438483586609703	9.502939259112843E-4
86-87	3.282833562238982E-4	0.0018141974949215428	0.0	0.12454033850851887	9.675719972914895E-4
88	3.282833562238982E-4	0.0019524220659631842	0.0	0.12455761657989907	9.848500686716946E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7685	0.0	50.491238	1
CTTATAC	5725	0.0	43.682	1
GTATCAA	14755	0.0	39.375114	1
TATACAC	7405	0.0	36.055252	3
TTATACA	7790	0.0	32.971733	2
ATCAACG	17925	0.0	31.843225	3
TCAACGC	18050	0.0	31.646147	4
TATCAAC	18390	0.0	31.177988	2
CAACGCA	18420	0.0	31.033445	5
AACGCAG	19135	0.0	29.940184	6
TACACAT	10070	0.0	26.681396	5
ACGCAGA	21895	0.0	26.146715	7
CGCAGAG	22235	0.0	25.765928	8
ACACATC	10610	0.0	23.768139	6
ACATCTC	10720	0.0	23.682129	8
ATACACA	11960	0.0	23.66787	4
GTGGTAT	3785	0.0	23.58887	1
GCAGAGT	25850	0.0	22.179054	9
TGGTATC	3735	0.0	22.091974	2
CACATCT	12075	0.0	20.70926	7
>>END_MODULE