FastQCFastQC Report
Fri 27 May 2016
SRR2140122_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140122_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5605609
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65900.048.4000051
GTATCAA120850.037.00681
TCAACGC137300.031.9736274
ATCAACG137750.031.8379333
CAACGCA139850.031.359855
TATCAAC145500.030.4118442
AACGCAG144300.030.362936
CTTATAC47000.030.3139691
ACGCAGA165900.026.3578267
CGCAGAG169850.025.7955328
TATACAC69100.023.917483
GTGGTAT37050.022.6547371
GCAGAGT200050.021.901389
TGGTATC33650.021.7458552
TTATACA72850.020.7391152
GAGTACT120450.020.49221212-13
ACATCTC72500.019.7088998
CAGAGTA193850.019.3714410-11
GTACATG142500.019.1508051
TACATGG139200.018.677142
Produced by FastQC (version 0.11.5)