FastQCFastQC Report
Fri 27 May 2016
SRR2140121_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140121_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5645492
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68350.049.49991
GTATCAA132450.038.91061
CTTATAC51950.036.7001841
TCAACGC156450.032.4215774
ATCAACG157300.032.219333
TATCAAC162350.031.5347252
CAACGCA161300.031.3933985
AACGCAG167100.030.4056026
TATACAC72300.029.6048663
ACGCAGA188300.026.9371517
CGCAGAG192050.026.3668588
TTATACA80800.025.3343322
TGGTATC31800.023.286162
GCAGAGT227350.022.32919
ACATCTC88550.022.0571738
ACACATC87800.021.7609356
TACACAT97350.021.724325
GTGGTAT36550.021.657151
GAGTACT133000.019.74055112-13
GTACATG160600.019.3178041
Produced by FastQC (version 0.11.5)