##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140121_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5645492
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.603130781161322	33.0	32.0	33.0	28.0	34.0
2	32.42351313224782	33.0	33.0	34.0	31.0	34.0
3	32.60020224986591	33.0	33.0	34.0	31.0	34.0
4	32.979065774958144	33.0	33.0	34.0	31.0	34.0
5	33.1327083627078	34.0	33.0	34.0	33.0	34.0
6	36.60410146715291	38.0	37.0	38.0	34.0	38.0
7	37.15808241336627	38.0	38.0	38.0	36.0	38.0
8	37.10591707507512	38.0	38.0	38.0	36.0	38.0
9	37.258802067206894	38.0	38.0	38.0	36.0	38.0
10-11	37.36376944648934	38.0	38.0	38.0	37.0	38.0
12-13	37.395247748114784	38.0	38.0	38.0	37.0	38.0
14-15	37.41662471579093	38.0	38.0	38.0	37.0	38.0
16-17	37.42688192632281	38.0	38.0	38.0	37.0	38.0
18-19	37.44412781029537	38.0	38.0	38.0	37.0	38.0
20-21	37.45386164796437	38.0	38.0	38.0	37.0	38.0
22-23	37.47206532973767	38.0	38.0	38.0	37.0	38.0
24-25	37.47978436674359	38.0	38.0	38.0	37.0	38.0
26-27	37.452002824048094	38.0	38.0	38.0	37.0	38.0
28-29	37.43227143783108	38.0	38.0	38.0	37.0	38.0
30-31	37.4274419938361	38.0	38.0	38.0	37.0	38.0
32-33	37.41652496346549	38.0	38.0	38.0	37.0	38.0
34-35	37.38730303673201	38.0	38.0	38.0	37.0	38.0
36-37	37.36469052556336	38.0	38.0	38.0	37.0	38.0
38-39	37.33562645191054	38.0	38.0	38.0	37.0	38.0
40-41	37.34709422441199	38.0	38.0	38.0	37.0	38.0
42-43	37.31269613804591	38.0	38.0	38.0	37.0	38.0
44-45	37.267834339969994	38.0	38.0	38.0	37.0	38.0
46-47	37.23429706864983	38.0	38.0	38.0	37.0	38.0
48-49	37.1894953057285	38.0	38.0	38.0	37.0	38.0
50-51	37.156518415120686	38.0	38.0	38.0	37.0	38.0
52-53	37.11954185931039	38.0	38.0	38.0	36.0	38.0
54-55	37.10194654848125	38.0	38.0	38.0	36.0	38.0
56-57	37.056747276655386	38.0	38.0	38.0	36.0	38.0
58-59	37.02335047851897	38.0	38.0	38.0	36.0	38.0
60-61	37.042145534569435	38.0	38.0	38.0	36.0	38.0
62-63	37.052656320625786	38.0	38.0	38.0	36.0	38.0
64-65	37.058524062717325	38.0	38.0	38.0	36.0	38.0
66-67	37.04957139753574	38.0	38.0	38.0	36.0	38.0
68-69	37.00670542505005	38.0	38.0	38.0	36.0	38.0
70-71	36.99970227231108	38.0	38.0	38.0	36.0	38.0
72-73	37.00672913107759	38.0	38.0	38.0	36.0	38.0
74-75	37.022390841403464	38.0	38.0	38.0	36.0	38.0
76-77	37.00149656540002	38.0	38.0	38.0	36.0	38.0
78-79	36.96131859875659	38.0	38.0	38.0	36.0	38.0
80-81	36.91432734904712	38.0	38.0	38.0	35.5	38.0
82-83	36.868773407152325	38.0	38.0	38.0	35.0	38.0
84-85	36.839700373748	38.0	38.0	38.0	35.0	38.0
86-87	36.86213858229208	38.0	38.0	38.0	35.0	38.0
88-89	36.81513630412131	38.0	38.0	38.0	35.0	38.0
90-91	36.82958410975469	38.0	38.0	38.0	35.0	38.0
92-93	36.81434105142119	38.0	38.0	38.0	35.0	38.0
94-95	36.82517078383409	38.0	38.0	38.0	35.0	38.0
96-97	36.82337136637116	38.0	38.0	38.0	35.0	38.0
98-99	36.803975462975416	38.0	38.0	38.0	35.0	38.0
100	35.30207011515018	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	7.0
20	14.0
21	65.0
22	271.0
23	924.0
24	2131.0
25	4478.0
26	8254.0
27	13906.0
28	21921.0
29	32861.0
30	43984.0
31	58071.0
32	74861.0
33	99743.0
34	145338.0
35	252494.0
36	666588.0
37	4219576.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.19493925418723	19.638199823859463	14.249989194918708	24.916871727034597
2	14.240986594573949	20.129521007526545	43.10205948570343	22.52743291219608
3	18.145786112632486	25.473157622138505	30.337477300408327	26.04357896482068
4	12.393858744027243	17.87649291240252	38.88013745643597	30.849510887134272
5	13.529166279927418	36.955804737656166	35.64847846742144	13.866550514994975
6	30.01741920810445	37.48068370303243	18.748002831285564	13.753894257577551
7	26.659129089191875	32.44174289858174	22.682345489108833	18.216782523117562
8	24.152261662933896	36.76069331069816	21.166197737947375	17.920847288420568
9	25.267842023334726	17.22452179544316	21.167650224285147	36.33998595693697
10-11	24.160764021984267	26.611560161629843	28.838965673850925	20.388710142534965
12-13	25.10129320881156	23.96366871124784	28.93009147829808	22.004946601642512
14-15	22.24636931555301	25.41436601096946	26.562069346657474	25.777195326820053
16-17	21.886896660202513	28.720499471082412	27.16943005144636	22.223173817268716
18-19	21.862248675580446	27.634801360094034	29.024644796237425	21.478305168088095
20-21	22.58524752750596	26.741887617433836	28.994061196231346	21.67880365882886
22-23	22.27460031332969	26.831600525192144	28.869899801104378	22.023899360373786
24-25	22.160994861206515	26.96400187251735	28.96820662174928	21.906796644526853
26-27	22.128257814302867	27.132379289068858	28.9201027423199	21.81926015430837
28-29	21.92028595403067	27.18666155173625	28.956859921888313	21.936192572344762
30-31	22.56423474798379	26.833465257941285	28.864249755812338	21.73805023826258
32-33	21.848360042387537	27.209446832683337	28.92026066700295	22.021932457926173
34-35	22.163809794666417	27.17665812309181	28.967966628292302	21.691565453949472
36-37	21.996058475221755	27.15587516936868	28.924472769415253	21.923593585994304
38-39	21.853906979555013	26.93671788105389	29.196472380058907	22.01290275933219
40-41	22.213567951732337	26.76891936583541	29.063063120124905	21.954449562307353
42-43	22.20774070123986	26.817702000829257	28.91805466203104	22.056502635899843
44-45	22.021978243730935	27.11791120004366	28.799506886030223	22.060603670195178
46-47	22.32898372256966	26.866536137323948	28.383682987090697	22.42079715301569
48-49	22.188890646456812	26.972240847776106	28.424352798942472	22.414515706824606
50-51	22.026845281482405	27.15818918624994	28.529306509752157	22.285659022515507
52-53	22.21503000148152	27.089854870801165	28.59554507170109	22.099570056016223
54-55	22.134714774506083	27.042507530440535	28.393149121013227	22.429628574040155
56-57	22.37193291139716	27.311576424559618	28.079679830231335	22.236810833811887
58-59	22.03775758207173	27.513281454090837	28.452439449164668	21.99652151467277
60-61	22.2776632769881	27.711746938150654	28.074298832261825	21.936290952599418
62-63	22.002476549578137	27.898218319468963	28.08317869724557	22.016126433707324
64-65	21.95389550321464	27.808390618361212	28.104771398511847	22.132942479912305
66-67	22.057393101437963	27.73915966224042	27.957891755413684	22.24555548090793
68-69	22.073574303340614	27.57372157742563	28.162481814807826	22.190222304425923
70-71	22.130272752624357	27.253837216564136	28.268201285348262	22.34768874546324
72-73	22.276752181311366	27.18783858478432	28.22905378334058	22.30635545056374
74-75	22.086804691602495	27.29450283845763	28.224334236822756	22.394358233117117
76-77	22.260411684912338	27.277607575591002	28.048865547549777	22.413115191946883
78-79	22.256330874817653	27.349626281267657	27.99023325462932	22.40380958928537
80-81	22.34021799629396	27.328558039941854	27.947310306823876	22.383913656940322
82-83	22.36504397614534	27.345660501327067	28.010539308387788	22.278756214139804
84-85	22.47996685713032	27.314868009673837	27.92618066210173	22.278984471094116
86-87	22.24896738579478	27.38720912303489	27.986233899285963	22.37758959188437
88-89	22.3482866133797	27.369145606765343	27.91469546955238	22.367872310302577
90-91	22.434563887695287	27.381528718207544	27.931467168002218	22.252440226094954
92-93	22.43932720207709	27.40011118027762	27.86785225047566	22.292709367169632
94-95	22.25992296650197	27.487316350688033	27.89876519730842	22.353995485501567
96-97	22.378289341901592	27.56659283148472	27.942734997317515	22.112382829296173
98-99	23.44783072960027	29.470422488127788	28.208493220608116	18.873253561663827
100	29.839268104465738	40.66448984280123	29.49624205273303	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1049.0
1	962.5
2	1173.5
3	1647.0
4	2154.0
5	3004.0
6	3828.0
7	4957.0
8	6339.0
9	7074.5
10	7032.5
11	7026.5
12	7782.0
13	9598.5
14	13278.5
15	17529.5
16	20634.0
17	22340.0
18	22188.5
19	20641.0
20	18875.0
21	17511.0
22	17794.0
23	20653.0
24	25819.5
25	33354.5
26	43753.0
27	55597.5
28	66641.5
29	77852.5
30	89205.5
31	100732.5
32	113652.5
33	127732.5
34	141865.5
35	155284.0
36	168817.5
37	180830.5
38	190386.0
39	198179.0
40	207936.0
41	218103.0
42	224585.5
43	235367.5
44	248392.0
45	252512.5
46	249482.5
47	246861.5
48	243455.5
49	237342.0
50	231592.0
51	224735.5
52	214776.0
53	203223.0
54	190607.5
55	176938.0
56	161770.5
57	145603.5
58	129271.0
59	110999.5
60	92687.5
61	75630.0
62	59417.5
63	45257.5
64	33863.5
65	25414.0
66	19065.5
67	14459.0
68	10944.5
69	8209.5
70	6113.0
71	4410.0
72	3127.5
73	2078.5
74	1321.0
75	842.5
76	562.0
77	369.5
78	237.0
79	146.5
80	95.5
81	59.5
82	36.0
83	24.5
84	22.0
85	20.5
86	16.0
87	10.5
88	6.5
89	3.5
90	1.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00954744068364635
3	2.8341196834571727E-4
4	0.001186787617447691
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	8.85712526942268E-6
24-25	6.20038474273098E-5
26-27	5.935242780663014E-4
28-29	0.0013912558946714722
30-31	7.894126459470979E-4
32-33	8.531467785710858E-4
34-35	0.0011404370742906548
36-37	0.0
38-39	1.6187326941737314E-4
40-41	9.59118826769658E-4
42-43	3.915002919408572E-4
44-45	0.0
46-47	0.0
48-49	0.0018197496024547023
50-51	0.003744102802903091
52-53	0.0
54-55	0.003820352142868945
56-57	0.03737436109199004
58-59	0.053365556001293835
60-61	0.02872774591739245
62-63	0.006602114851101555
64-65	0.0
66-67	0.0
68-69	3.055478008655354E-5
70-71	0.0035346755276070008
72-73	0.007551496985189992
74-75	0.005548881221301386
76-77	0.00663421994437887
78-79	0.009935483801670558
80-81	0.010648665464955905
82-83	0.040219984780678786
84-85	0.024104540149955497
86-87	0.0013315214198717823
88-89	0.0015039598811351527
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	43.0
20-21	198.0
22-23	357.0
24-25	442.0
26-27	1376.0
28-29	3532.0
30-31	10320.0
32-33	13325.0
34-35	18183.0
36-37	29057.0
38-39	34483.0
40-41	33562.0
42-43	38223.0
44-45	46503.0
46-47	46794.0
48-49	43872.0
50-51	40570.0
52-53	39004.0
54-55	42599.0
56-57	44442.0
58-59	44133.0
60-61	44650.0
62-63	43995.0
64-65	48837.0
66-67	54057.0
68-69	54333.0
70-71	63152.0
72-73	45746.0
74-75	45061.0
76-77	47489.0
78-79	48727.0
80-81	48270.0
82-83	45421.0
84-85	44811.0
86-87	46314.0
88-89	48393.0
90-91	48895.0
92-93	48340.0
94-95	56804.0
96-97	174061.0
98-99	1306015.0
100-101	2751103.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.48426053262789
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.04874919966159	79.65332496153088
2	6.557467698230606	11.473504250925961
3	1.4203533610555996	3.7277569046094534
4	0.4666675097316055	1.6330424801388976
5	0.21511649509206057	0.9409653750749798
6	0.09915491364345899	0.5204696578964751
7	0.05966410261770846	0.36537689284971364
8	0.03395800930540824	0.23766330665949895
9	0.02428852287093437	0.1912377116514162
>10	0.07219810428744503	0.9765114473938621
>50	0.0014463521843087203	0.08316028501180084
>100	8.768100437359257E-4	0.13716661580162082
>500	2.9460637811112884E-5	0.0224486498105429
>1k	2.946063780791383E-5	0.03737146064495135
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.542649604321466E-5	0.0	1.771324802160733E-5	0.0
3	0.0	3.542649604321466E-5	0.0	5.3139744064821984E-5	0.0
4	0.0	5.3139744064821984E-5	0.0	7.085299208642932E-5	0.0
5	0.0	5.3139744064821984E-5	0.0	7.085299208642932E-5	0.0
6	8.856624010803665E-5	5.3139744064821984E-5	0.0	7.085299208642932E-5	0.0
7	8.856624010803665E-5	5.3139744064821984E-5	0.0	7.085299208642932E-5	0.0
8	8.856624010803665E-5	7.085299208642932E-5	0.0	7.085299208642932E-5	0.0
9	8.856624010803665E-5	1.5941923219446597E-4	0.0	7.085299208642932E-5	0.0
10-11	8.856624010803665E-5	1.5941923219446597E-4	0.0	7.970961609723298E-5	0.0
12-13	8.856624010803665E-5	1.5941923219446597E-4	0.0	9.742286411884032E-5	0.0
14-15	8.856624010803665E-5	2.3027222428089527E-4	0.0	1.0627948812964397E-4	0.0
16-17	1.0627948812964397E-4	2.3027222428089527E-4	0.0	1.1513611214044763E-4	0.0
18-19	1.0627948812964397E-4	2.479854723025026E-4	0.0	1.5941923219446597E-4	0.0
20-21	1.0627948812964397E-4	2.656987203241099E-4	0.0	1.771324802160733E-4	0.0
22-23	1.0627948812964397E-4	3.3655171241053924E-4	0.0	2.0370235224848428E-4	0.0
24-25	1.0627948812964397E-4	3.6312158444295025E-4	0.0	2.656987203241099E-4	1.771324802160733E-5
26-27	1.0627948812964397E-4	3.8083483246455755E-4	0.0	3.808348324645576E-4	1.771324802160733E-5
28-29	1.0627948812964397E-4	3.896914564753612E-4	0.0	7.085299208642931E-4	1.771324802160733E-5
30-31	1.0627948812964397E-4	5.136841926266125E-4	0.0	0.001815607922214751	2.6569872032410992E-5
32-33	1.0627948812964397E-4	5.136841926266125E-4	0.0	0.004268892773207366	3.542649604321466E-5
34-35	1.0627948812964397E-4	5.579673126806309E-4	0.0	0.007519273785172311	3.542649604321466E-5
36-37	1.0627948812964397E-4	6.111070567454528E-4	0.0	0.012514409727265578	3.542649604321466E-5
38-39	1.0627948812964397E-4	6.553901767994712E-4	0.0	0.01994511727232985	3.542649604321466E-5
40-41	1.0627948812964397E-4	6.731034248210785E-4	0.0	0.02781865601793431	3.542649604321466E-5
42-43	1.0627948812964397E-4	6.996732968534895E-4	0.0	0.035709908011560376	3.542649604321466E-5
44-45	1.239927361512513E-4	8.236660330047408E-4	0.0	0.044823374118677345	3.542649604321466E-5
46-47	1.4170598417285863E-4	9.210888971235811E-4	0.0	0.05410511608199958	3.542649604321466E-5
48-49	1.4170598417285863E-4	9.210888971235811E-4	0.0	0.0633160050532354	5.3139744064821984E-5
50-51	1.4170598417285863E-4	0.0010716515053072434	0.0	0.07242061453634156	5.3139744064821984E-5
52-53	1.4170598417285863E-4	0.001080508129318047	0.0	0.08167578662763139	6.199636807562565E-5
54-55	1.4170598417285863E-4	0.0010982213773396542	0.0	0.09005415294185165	7.970961609723298E-5
56-57	1.4170598417285863E-4	0.0011513611214044764	0.0	0.09671433419797601	8.856624010803665E-5
58-59	1.4170598417285863E-4	0.0011513611214044764	0.0	0.10317081310185189	8.856624010803665E-5
60-61	1.4170598417285863E-4	0.001186787617447691	0.0	0.10957415226166292	1.0627948812964397E-4
62-63	1.5941923219446597E-4	0.0012310707375017093	0.0	0.11582692881329032	1.0627948812964397E-4
64-65	1.5941923219446597E-4	0.001346206849642157	0.0	0.12124718270790216	1.0627948812964397E-4
66-67	1.5941923219446597E-4	0.0013550634736529607	0.0	0.12716340754711902	1.1513611214044763E-4
68-69	1.5941923219446597E-4	0.0013904899696961753	0.0	0.13284050353804416	1.239927361512513E-4
70-71	1.5941923219446597E-4	0.0014082032177177826	0.0	0.13725995891943518	1.4170598417285863E-4
72-73	1.5941923219446597E-4	0.0014170598417285864	0.0	0.14149342519659935	2.1255897625928794E-4
74-75	1.5941923219446597E-4	0.00142591646573939	0.0	0.14462867009642383	2.1255897625928794E-4
76-77	1.5941923219446597E-4	0.0014701995857934083	0.0	0.14794104747646442	2.1255897625928794E-4
78-79	1.5941923219446597E-4	0.001505626081836623	0.0	0.1495883795424739	2.1255897625928794E-4
80-81	1.5941923219446597E-4	0.001505626081836623	0.0	0.1501729167271869	2.3027222428089527E-4
82-83	1.5941923219446597E-4	0.001505626081836623	0.0	0.15074859728788917	2.8341196834571727E-4
84-85	1.5941923219446597E-4	0.001505626081836623	0.0	0.15100543938420247	2.8341196834571727E-4
86-87	1.5941923219446597E-4	0.001505626081836623	0.0	0.15108514900029973	2.8341196834571727E-4
88	1.5941923219446597E-4	0.0015587658259014448	0.0	0.15109400562431052	2.8341196834571727E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6835	0.0	49.4999	1
GTATCAA	13245	0.0	38.9106	1
CTTATAC	5195	0.0	36.700184	1
TCAACGC	15645	0.0	32.421577	4
ATCAACG	15730	0.0	32.21933	3
TATCAAC	16235	0.0	31.534725	2
CAACGCA	16130	0.0	31.393398	5
AACGCAG	16710	0.0	30.405602	6
TATACAC	7230	0.0	29.604866	3
ACGCAGA	18830	0.0	26.937151	7
CGCAGAG	19205	0.0	26.366858	8
TTATACA	8080	0.0	25.334332	2
TGGTATC	3180	0.0	23.28616	2
GCAGAGT	22735	0.0	22.3291	9
ACATCTC	8855	0.0	22.057173	8
ACACATC	8780	0.0	21.760935	6
TACACAT	9735	0.0	21.72432	5
GTGGTAT	3655	0.0	21.65715	1
GAGTACT	13300	0.0	19.740551	12-13
GTACATG	16060	0.0	19.317804	1
>>END_MODULE