FastQCFastQC Report
Fri 27 May 2016
SRR2140120_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140120_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5901604
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72300.051.5370671
GTATCAA133000.037.922941
TCAACGC156300.031.500914
ATCAACG158300.031.0218523
CAACGCA160600.030.6569655
TATCAAC164500.030.1929932
AACGCAG164800.029.9016156
ACGCAGA191550.025.7481787
CGCAGAG192700.025.5723198
GTGGTAT34550.022.2876511
TGGTATC32950.022.2014082
GCAGAGT230100.021.2671269
GAGTACT132600.020.25888312-13
GTACATG168450.019.7074551
TACATGG165900.019.5977882
CAGAGTA222800.019.07445110-11
ACATGGG169950.018.7769223
AGAGTAC205650.018.15870710-11
GTACTTT151800.017.72504814-15
CATGGGG124050.016.0347774
Produced by FastQC (version 0.11.5)