##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140120_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5901604
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.787750584417388	33.0	32.0	34.0	28.0	34.0
2	32.47051920122055	33.0	33.0	34.0	31.0	34.0
3	32.64621550344618	33.0	33.0	34.0	31.0	34.0
4	32.99893893253427	33.0	33.0	34.0	31.0	34.0
5	33.15082916441022	34.0	33.0	34.0	33.0	34.0
6	36.63570649606446	38.0	37.0	38.0	34.0	38.0
7	37.17566749649756	38.0	38.0	38.0	36.0	38.0
8	37.131051151517454	38.0	38.0	38.0	36.0	38.0
9	37.266818139610855	38.0	38.0	38.0	36.0	38.0
10-11	37.368272168041095	38.0	38.0	38.0	37.0	38.0
12-13	37.398913922384494	38.0	38.0	38.0	37.0	38.0
14-15	37.419992090285966	38.0	38.0	38.0	37.0	38.0
16-17	37.43233568365481	38.0	38.0	38.0	37.0	38.0
18-19	37.43890686328666	38.0	38.0	38.0	37.0	38.0
20-21	37.4466292044704	38.0	38.0	38.0	37.0	38.0
22-23	37.461157539844265	38.0	38.0	38.0	37.0	38.0
24-25	37.47016294929645	38.0	38.0	38.0	37.0	38.0
26-27	37.43905758536137	38.0	38.0	38.0	37.0	38.0
28-29	37.418183679632286	38.0	38.0	38.0	37.0	38.0
30-31	37.417204274831086	38.0	38.0	38.0	37.0	38.0
32-33	37.410583939325385	38.0	38.0	38.0	37.0	38.0
34-35	37.38570362222704	38.0	38.0	38.0	37.0	38.0
36-37	37.361916764091866	38.0	38.0	38.0	37.0	38.0
38-39	37.35240486901682	38.0	38.0	38.0	37.0	38.0
40-41	37.34204430806672	38.0	38.0	38.0	37.0	38.0
42-43	37.30270565831735	38.0	38.0	38.0	37.0	38.0
44-45	37.25956995321163	38.0	38.0	38.0	37.0	38.0
46-47	37.23239483052669	38.0	38.0	38.0	37.0	38.0
48-49	37.191621030383324	38.0	38.0	38.0	37.0	38.0
50-51	37.16271134579213	38.0	38.0	38.0	37.0	38.0
52-53	37.12614976323624	38.0	38.0	38.0	36.5	38.0
54-55	37.11293073444668	38.0	38.0	38.0	36.0	38.0
56-57	37.06842534205951	38.0	38.0	38.0	36.0	38.0
58-59	37.042045596879575	38.0	38.0	38.0	36.0	38.0
60-61	37.06617124837791	38.0	38.0	38.0	36.0	38.0
62-63	37.07775140958735	38.0	38.0	38.0	36.0	38.0
64-65	37.08123054962489	38.0	38.0	38.0	36.0	38.0
66-67	37.07249913928783	38.0	38.0	38.0	36.0	38.0
68-69	37.03006991737786	38.0	38.0	38.0	36.0	38.0
70-71	37.025579591707235	38.0	38.0	38.0	36.0	38.0
72-73	37.03631631428084	38.0	38.0	38.0	36.0	38.0
74-75	37.04845819416997	38.0	38.0	38.0	36.0	38.0
76-77	37.0268180329771	38.0	38.0	38.0	36.0	38.0
78-79	36.98354397117669	38.0	38.0	38.0	36.0	38.0
80-81	36.933116260963004	38.0	38.0	38.0	36.0	38.0
82-83	36.88693186446586	38.0	38.0	38.0	35.0	38.0
84-85	36.85542325137685	38.0	38.0	38.0	35.0	38.0
86-87	36.87706395932872	38.0	38.0	38.0	35.0	38.0
88-89	36.831995681887385	38.0	38.0	38.0	35.0	38.0
90-91	36.84170001982896	38.0	38.0	38.0	35.0	38.0
92-93	36.82882109221755	38.0	38.0	38.0	35.0	38.0
94-95	36.84399444679803	38.0	38.0	38.0	35.0	38.0
96-97	36.838194649700995	38.0	38.0	38.0	35.0	38.0
98-99	36.820571543396866	38.0	38.0	38.0	35.0	38.0
100	35.25281533452693	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	19.0
21	68.0
22	316.0
23	961.0
24	2416.0
25	5050.0
26	9440.0
27	15133.0
28	23066.0
29	33960.0
30	46767.0
31	61106.0
32	78837.0
33	104111.0
34	150885.0
35	260064.0
36	670326.0
37	4439074.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.436717882121535	19.722519504866813	14.176400178663293	24.66436243434836
2	14.387555323878445	20.420719909885438	42.68669039285821	22.505034373377907
3	18.130252716683593	25.65680192232973	30.196392869784734	26.016552491201946
4	12.426902492317677	17.843239089491124	39.225049076214916	30.50480934197628
5	13.623516589727133	37.09813467660656	35.47908331362118	13.799265420045126
6	29.989169046245735	37.62312754295273	18.916264120737345	13.471439290064193
7	26.75938609232337	32.56392668840539	22.98981768346368	17.686869535807553
8	24.291429923119207	36.640072766658015	21.19235719645032	17.87614011377246
9	25.62325428815624	17.042925957078786	21.332810537609774	36.0010092171552
10-11	24.201589940633088	26.637139665758664	28.900431137026477	20.260839256581768
12-13	25.158694144846045	24.177088127227783	28.959228711380835	21.70498901654533
14-15	22.306901649110987	25.554188318972265	26.713440956051947	25.425469075864797
16-17	21.828955314521274	28.64423807493692	27.381505095902742	22.145301514639073
18-19	21.808282968494666	27.750184526105105	29.15972505102003	21.2818074543802
20-21	22.62536428044144	26.95768886432276	29.04305433259146	21.373892522644333
22-23	22.34052783475876	26.824419658906024	28.914573658792996	21.92047884754222
24-25	22.122084675727116	26.923843091194204	28.954161878984973	21.999910354093704
26-27	22.024191559731918	27.12371438909702	28.917498036151756	21.93459601501931
28-29	21.9678469652595	27.140561090876204	28.98458109245453	21.907010851409765
30-31	22.277738406836896	26.96247219229927	28.85236502368379	21.907424377180043
32-33	22.054451517327326	27.099750712683594	28.825423821163916	22.020373948825164
34-35	22.188535215079327	27.130504374595883	28.825321563218015	21.855638847106775
36-37	22.193052063217916	27.03761599328582	28.765325771497686	22.00400617199858
38-39	22.015678672089336	27.21672056921365	28.855628563486018	21.911972195211
40-41	22.259670301568974	27.070788721779277	28.75800256968088	21.911538406970873
42-43	22.173067240672122	27.11331408065928	28.594477611628484	22.11914106704011
44-45	22.21963552875215	27.261048491208008	28.44356198739079	22.075753992649055
46-47	22.276969576118947	27.203674334351867	28.22960875749367	22.289747332035514
48-49	22.190606945243967	27.30125025806443	28.267782367076194	22.240360429615407
50-51	22.223080215290135	27.46012706947254	28.110873360133283	22.205919355104044
52-53	22.39511781727912	27.420049811463738	27.93405767820476	22.250774693052385
54-55	22.317506177442596	27.293423755452572	28.023949287254663	22.365120779850173
56-57	22.34747055480522	27.410125143515845	27.978804521640694	22.26359978003824
58-59	22.287573126408606	27.470234009148943	28.00749456051192	22.234698303930532
60-61	22.336822537633587	27.392841576497766	28.058140489304723	22.21219539656392
62-63	22.271592008114546	27.40910055905456	28.057934092892694	22.2613733399382
64-65	22.222974893149537	27.34618389972825	28.041740634820634	22.389100572301583
66-67	22.327898185532256	27.380800162804853	27.91484039172997	22.37646125993292
68-69	22.25963379846911	27.33551408017631	28.040276219320955	22.364575902033625
70-71	22.31726066262534	27.126619198638817	28.07974581725054	22.476374321485302
72-73	22.34766256357268	27.241899667739446	28.02577507714038	22.3846626915475
74-75	22.240086508539996	27.381553221694777	27.9523445721561	22.426015697609127
76-77	22.37729358271142	27.330817609556757	27.835860239818295	22.456028567913528
78-79	22.336590272705724	27.3535766611422	27.81783097855191	22.492002087600167
80-81	22.412911352937208	27.38357450657866	27.75891002344218	22.44460411704195
82-83	22.42409252218786	27.347267247834107	27.847523401219163	22.38111682875887
84-85	22.539887128692136	27.316971530882554	27.767064194247542	22.376077146177767
86-87	22.318769947701213	27.362352272042507	27.856151371534953	22.462726408721327
88-89	22.435958371312527	27.388983081803715	27.752152130683612	22.422906416200146
90-91	22.480890830891884	27.39062062219314	27.764420208995656	22.364068337919317
92-93	22.454585574641385	27.355181363523783	27.790356889340412	22.399876172494423
94-95	22.36591511899735	27.48267686762218	27.743253872968292	22.40815414041218
96-97	22.4544404429641	27.58659136914116	27.782429096737037	22.1765390911577
98-99	23.583505809399867	29.507520433607436	27.978773768855667	18.930199988137026
100	22.46151707004595	30.298045652326167	21.916395140413343	25.324042137214548
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	907.0
1	873.0
2	1153.0
3	1613.5
4	2177.5
5	3132.0
6	3917.5
7	4920.0
8	6060.0
9	6719.0
10	6732.0
11	6648.5
12	7433.0
13	9332.5
14	12985.5
15	17240.0
16	20430.5
17	22226.5
18	22197.0
19	20646.0
20	18800.0
21	17628.5
22	18285.5
23	21131.5
24	26050.5
25	33669.5
26	44170.0
27	55703.0
28	66429.5
29	77934.0
30	90072.0
31	103095.5
32	117376.0
33	131150.5
34	144961.0
35	158790.5
36	174980.5
37	189629.5
38	199635.5
39	208320.5
40	218428.0
41	227244.5
42	232988.5
43	239780.0
44	245467.0
45	250443.0
46	253571.5
47	255423.5
48	255519.5
49	251628.0
50	246735.5
51	239793.5
52	229475.5
53	216873.0
54	203193.5
55	188008.0
56	171898.0
57	155128.5
58	137730.5
59	119927.0
60	100413.0
61	81160.5
62	63924.0
63	49078.0
64	37147.5
65	27761.0
66	20449.5
67	15289.0
68	11581.5
69	8371.5
70	5909.5
71	4061.5
72	2657.0
73	1663.0
74	1046.5
75	660.5
76	428.5
77	267.0
78	163.5
79	113.0
80	85.0
81	58.0
82	40.5
83	28.5
84	16.0
85	8.0
86	5.0
87	4.5
88	4.0
89	2.0
90	1.5
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.011369790314633107
3	2.541681888517088E-4
4	0.0011691736687178605
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	8.472657716828867E-6
24-25	5.083886241266836E-5
26-27	6.270716668344507E-4
28-29	0.0014833721609528302
30-31	7.211433634703387E-4
32-33	7.56517997563162E-4
34-35	0.0010566133429132942
36-37	0.0
38-39	2.4935580218277473E-4
40-41	9.169932517947548E-4
42-43	4.178281709502179E-4
44-45	0.0
46-47	0.0
48-49	0.001863825851246721
50-51	0.0037214938291969166
52-53	0.0
54-55	0.00398749550608344
56-57	0.04019358422446438
58-59	0.05744607124815784
60-61	0.030305270160396188
62-63	0.007057270885417659
64-65	0.0
66-67	0.0
68-69	2.906714238597832E-5
70-71	0.003355105982222438
72-73	0.007652229756075549
74-75	0.005500148314687797
76-77	0.00661865226737625
78-79	0.010331273241555326
80-81	0.01105224561722533
82-83	0.04313338041620136
84-85	0.025593665750912396
86-87	0.0013003168438709565
88-89	0.0015486940930777968
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	39.0
20-21	162.0
22-23	311.0
24-25	461.0
26-27	1256.0
28-29	3447.0
30-31	10653.0
32-33	13419.0
34-35	18386.0
36-37	29578.0
38-39	35614.0
40-41	34979.0
42-43	39662.0
44-45	47183.0
46-47	48178.0
48-49	45406.0
50-51	41643.0
52-53	40734.0
54-55	44133.0
56-57	45627.0
58-59	45192.0
60-61	45180.0
62-63	43703.0
64-65	46005.0
66-67	48101.0
68-69	48634.0
70-71	48524.0
72-73	45866.0
74-75	46806.0
76-77	48206.0
78-79	49698.0
80-81	49569.0
82-83	47104.0
84-85	46706.0
86-87	47701.0
88-89	50065.0
90-91	50592.0
92-93	50536.0
94-95	58607.0
96-97	183743.0
98-99	402168.0
100-101	3898027.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.34784117496113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.7715116018234	79.28695578607213
2	6.759893397352003	11.809241896631423
3	1.4765613465838268	3.869233379594725
4	0.49393743437575255	1.7257747427288406
5	0.22005037283596007	0.9610462508483211
6	0.10448601733834686	0.5475976828484495
7	0.05432039304107064	0.33213383447390316
8	0.03511337144689277	0.24536617538084837
9	0.017812212034433346	0.14002724409825734
>10	0.06400882344115989	0.830694577600725
>50	0.0015630693972978158	0.09198535626829378
>100	6.861101902822401E-4	0.11769622375991942
>500	2.79250701422769E-5	0.01714753797885838
>1k	2.792506943893975E-5	0.025099311715266862
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.6944545923447252E-5	0.0	0.0	0.0
3	0.0	1.6944545923447252E-5	0.0	1.6944545923447252E-5	0.0
4	0.0	1.6944545923447252E-5	0.0	3.3889091846894504E-5	0.0
5	0.0	1.6944545923447252E-5	0.0	5.083363777034176E-5	0.0
6	0.0	1.6944545923447252E-5	0.0	5.083363777034176E-5	0.0
7	0.0	1.6944545923447252E-5	0.0	5.083363777034176E-5	0.0
8	0.0	3.3889091846894504E-5	0.0	8.472272961723626E-5	0.0
9	0.0	8.472272961723626E-5	0.0	1.0166727554068352E-4	0.0
10-11	2.5416818885170876E-5	8.472272961723626E-5	0.0	1.0166727554068352E-4	0.0
12-13	5.083363777034176E-5	8.472272961723626E-5	0.0	1.270840944258544E-4	0.0
14-15	6.777818369378901E-5	9.319500257895989E-5	0.0	1.3555636738757802E-4	2.5416818885170876E-5
16-17	8.472272961723626E-5	1.0166727554068352E-4	0.0	1.4402864034930164E-4	3.3889091846894504E-5
18-19	8.472272961723626E-5	1.1861182146413076E-4	0.0	1.609731862727489E-4	3.3889091846894504E-5
20-21	8.472272961723626E-5	1.5250091331102527E-4	0.0	1.6944545923447253E-4	3.3889091846894504E-5
22-23	8.472272961723626E-5	1.5250091331102527E-4	0.0	1.7791773219619615E-4	5.083363777034175E-5
24-25	8.472272961723626E-5	1.5250091331102527E-4	0.0	2.202790970048143E-4	6.777818369378901E-5
26-27	8.472272961723626E-5	1.5250091331102527E-4	0.0	2.965295536603269E-4	6.777818369378901E-5
28-29	8.472272961723626E-5	1.609731862727489E-4	0.0	4.829195588182467E-4	6.777818369378901E-5
30-31	1.1861182146413077E-4	2.0333455108136704E-4	0.0	0.0012708409442585438	6.777818369378901E-5
32-33	1.1861182146413077E-4	2.0333455108136704E-4	0.0	0.003253352817301872	6.777818369378901E-5
34-35	1.1861182146413077E-4	2.3722364292826152E-4	0.0	0.006311843356484102	6.777818369378901E-5
36-37	1.1861182146413077E-4	2.7111273477515603E-4	0.0	0.010437840288843508	6.777818369378901E-5
38-39	1.1861182146413077E-4	2.880572806986033E-4	0.0	0.01665648864274865	6.777818369378901E-5
40-41	1.1861182146413077E-4	3.3889091846894505E-4	0.0	0.023264861552893076	6.777818369378901E-5
42-43	1.1861182146413077E-4	3.473631914306687E-4	0.0	0.029542815817530287	6.777818369378901E-5
44-45	1.1861182146413077E-4	3.558354643923923E-4	0.0	0.036701886470186754	6.777818369378901E-5
46-47	1.1861182146413077E-4	3.558354643923923E-4	0.0	0.044555683505704546	6.777818369378901E-5
48-49	1.1861182146413077E-4	3.558354643923923E-4	0.0	0.052206145990140986	6.777818369378901E-5
50-51	1.1861182146413077E-4	4.2361364808618133E-4	0.0	0.05987355302050087	6.777818369378901E-5
52-53	1.1861182146413077E-4	4.405581940096286E-4	0.0	0.06695637321650182	7.625045665551264E-5
54-55	1.1861182146413077E-4	4.405581940096286E-4	0.0	0.07459836342797653	9.319500257895989E-5
56-57	1.1861182146413077E-4	4.5750273993307584E-4	0.0	0.08221493682056608	1.0166727554068352E-4
58-59	1.1861182146413077E-4	4.5750273993307584E-4	0.0	0.08873858700109326	1.1861182146413077E-4
60-61	1.270840944258544E-4	4.5750273993307584E-4	0.0	0.09557571128120423	1.3555636738757802E-4
62-63	1.3555636738757802E-4	4.5750273993307584E-4	0.0	0.10161644190291316	1.3555636738757802E-4
64-65	1.3555636738757802E-4	5.422254695503121E-4	0.0	0.10814856435640209	1.3555636738757802E-4
66-67	1.3555636738757802E-4	5.422254695503121E-4	0.0	0.11464679771804412	1.3555636738757802E-4
68-69	1.3555636738757802E-4	5.591700154737593E-4	0.0	0.12092475198268132	1.3555636738757802E-4
70-71	1.3555636738757802E-4	5.591700154737593E-4	0.0	0.12599117121379205	1.4402864034930164E-4
72-73	1.3555636738757802E-4	5.591700154737593E-4	0.0	0.1301425849650366	1.5250091331102527E-4
74-75	1.3555636738757802E-4	5.676422884354829E-4	0.0	0.13342135460122367	1.5250091331102527E-4
76-77	1.3555636738757802E-4	6.100036532441011E-4	0.0	0.13670859651037243	1.5250091331102527E-4
78-79	1.3555636738757802E-4	6.100036532441011E-4	0.0	0.13868263611045406	1.5250091331102527E-4
80-81	1.3555636738757802E-4	6.100036532441011E-4	0.0	0.13934347340146847	1.6944545923447253E-4
82-83	1.3555636738757802E-4	6.100036532441011E-4	0.0	0.13991111568990394	1.8639000515791978E-4
84-85	1.3555636738757802E-4	6.269481991675483E-4	0.0	0.14029236797318154	1.8639000515791978E-4
86-87	1.3555636738757802E-4	6.269481991675483E-4	0.0	0.1403855629757605	1.8639000515791978E-4
88	1.3555636738757802E-4	6.777818369378901E-4	0.0	0.1403855629757605	1.8639000515791978E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7230	0.0	51.537067	1
GTATCAA	13300	0.0	37.92294	1
TCAACGC	15630	0.0	31.50091	4
ATCAACG	15830	0.0	31.021852	3
CAACGCA	16060	0.0	30.656965	5
TATCAAC	16450	0.0	30.192993	2
AACGCAG	16480	0.0	29.901615	6
ACGCAGA	19155	0.0	25.748178	7
CGCAGAG	19270	0.0	25.572319	8
GTGGTAT	3455	0.0	22.287651	1
TGGTATC	3295	0.0	22.201408	2
GCAGAGT	23010	0.0	21.267126	9
GAGTACT	13260	0.0	20.258883	12-13
GTACATG	16845	0.0	19.707455	1
TACATGG	16590	0.0	19.597788	2
CAGAGTA	22280	0.0	19.074451	10-11
ACATGGG	16995	0.0	18.776922	3
AGAGTAC	20565	0.0	18.158707	10-11
GTACTTT	15180	0.0	17.725048	14-15
CATGGGG	12405	0.0	16.034777	4
>>END_MODULE