FastQCFastQC Report
Fri 27 May 2016
SRR2140119_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140119_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6081308
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78700.049.3742181
CTTATAC62050.038.181331
GTATCAA142450.037.353121
TCAACGC168200.030.9943314
ATCAACG169200.030.7860893
CAACGCA171250.030.392295
TATACAC87400.030.0448683
TATCAAC178050.029.5477282
AACGCAG178050.029.2796786
TTATACA95200.026.0560952
ACGCAGA201800.025.833737
CGCAGAG206900.025.1969418
GTGGTAT38600.023.4187031
ACATCTC102700.023.232858
ACACATC103750.023.1628766
TACACAT116100.022.3592955
TGGTATC37500.021.7065122
GCAGAGT244000.021.3657679
CACATCT113250.021.2955047
GTACATG177450.020.3767151
Produced by FastQC (version 0.11.5)