##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140119_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6081308
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.542807731494605	33.0	32.0	33.0	28.0	34.0
2	32.40601627149949	33.0	33.0	34.0	31.0	34.0
3	32.59692585871329	33.0	33.0	34.0	31.0	34.0
4	32.979332406778276	33.0	33.0	34.0	31.0	34.0
5	33.12960517704415	34.0	33.0	34.0	33.0	34.0
6	36.61036161957263	38.0	37.0	38.0	34.0	38.0
7	37.14637722674135	38.0	38.0	38.0	36.0	38.0
8	37.094993379713706	38.0	38.0	38.0	36.0	38.0
9	37.24480095400529	38.0	38.0	38.0	36.0	38.0
10-11	37.352183609184074	38.0	38.0	38.0	37.0	38.0
12-13	37.38737102281286	38.0	38.0	38.0	37.0	38.0
14-15	37.408235777566276	38.0	38.0	38.0	37.0	38.0
16-17	37.415629992758134	38.0	38.0	38.0	37.0	38.0
18-19	37.43216812896173	38.0	38.0	38.0	37.0	38.0
20-21	37.44170973597593	38.0	38.0	38.0	37.0	38.0
22-23	37.4591797557557	38.0	38.0	38.0	37.0	38.0
24-25	37.46867754038807	38.0	38.0	38.0	37.0	38.0
26-27	37.43958174656736	38.0	38.0	38.0	37.0	38.0
28-29	37.41395187701049	38.0	38.0	38.0	37.0	38.0
30-31	37.41022287688307	38.0	38.0	38.0	37.0	38.0
32-33	37.39942033167654	38.0	38.0	38.0	37.0	38.0
34-35	37.37003784800096	38.0	38.0	38.0	37.0	38.0
36-37	37.34131205111842	38.0	38.0	38.0	37.0	38.0
38-39	37.308897117031286	38.0	38.0	38.0	37.0	38.0
40-41	37.33428117095433	38.0	38.0	38.0	37.0	38.0
42-43	37.301128800550494	38.0	38.0	38.0	37.0	38.0
44-45	37.25551206450119	38.0	38.0	38.0	37.0	38.0
46-47	37.22359576598586	38.0	38.0	38.0	37.0	38.0
48-49	37.18003584394499	38.0	38.0	38.0	37.0	38.0
50-51	37.14549288204205	38.0	38.0	38.0	37.0	38.0
52-53	37.10648950021782	38.0	38.0	38.0	36.0	38.0
54-55	37.088587886244284	38.0	38.0	38.0	36.0	38.0
56-57	37.04429261417708	38.0	38.0	38.0	36.0	38.0
58-59	37.007733968908084	38.0	38.0	38.0	36.0	38.0
60-61	37.024126716900795	38.0	38.0	38.0	36.0	38.0
62-63	37.03479308970681	38.0	38.0	38.0	36.0	38.0
64-65	37.040763220827486	38.0	38.0	38.0	36.0	38.0
66-67	37.03150035432141	38.0	38.0	38.0	36.0	38.0
68-69	36.98581746693674	38.0	38.0	38.0	36.0	38.0
70-71	36.9819118962547	38.0	38.0	38.0	36.0	38.0
72-73	36.98935731233408	38.0	38.0	38.0	36.0	38.0
74-75	37.003669733211524	38.0	38.0	38.0	36.0	38.0
76-77	36.98101593207532	38.0	38.0	38.0	36.0	38.0
78-79	36.94103371221181	38.0	38.0	38.0	36.0	38.0
80-81	36.894261992884495	38.0	38.0	38.0	35.0	38.0
82-83	36.8463812668017	38.0	38.0	38.0	35.0	38.0
84-85	36.815171136183295	38.0	38.0	38.0	35.0	38.0
86-87	36.837513816189286	38.0	38.0	38.0	35.0	38.0
88-89	36.78985380009731	38.0	38.0	38.0	35.0	38.0
90-91	36.79984919431805	38.0	38.0	38.0	35.0	38.0
92-93	36.78953310634107	38.0	38.0	38.0	35.0	38.0
94-95	36.80268269973224	38.0	38.0	38.0	35.0	38.0
96-97	36.794214856998124	38.0	38.0	38.0	35.0	38.0
98-99	36.77511947682874	38.0	38.0	38.0	35.0	38.0
100	35.156012695636605	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	5.0
19	10.0
20	16.0
21	101.0
22	311.0
23	1006.0
24	2540.0
25	5149.0
26	9545.0
27	15670.0
28	24423.0
29	35894.0
30	48691.0
31	63759.0
32	82543.0
33	110106.0
34	161868.0
35	280801.0
36	733283.0
37	4505586.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.02877209968645	19.880262601400883	14.178693136410786	24.912272162501882
2	14.202340261496726	20.156090889715113	43.19744739411067	22.444121454677497
3	18.19377885591107	25.655053949875462	30.24606773592392	25.905099458289545
4	12.372770288880941	17.94513440959675	38.824513153188704	30.857582148333602
5	13.484796362887721	37.03772280568588	35.66722816867687	13.810252662749528
6	29.88062765444539	37.572607735046475	18.74475030700632	13.802014303501812
7	26.507077095914234	32.51382761734811	22.655076835443953	18.324018451293703
8	24.05954442695552	36.92990060690891	21.113582801594657	17.896972164540916
9	25.122720309512363	17.30994384760647	21.18787602930159	36.37945981357958
10-11	24.090738374047163	26.758947252794957	28.76918912839146	20.38112524476642
12-13	25.047744662825828	24.033324738691082	28.91797290977533	22.00095768870776
14-15	22.157938719762264	25.532073034288018	26.44145798897211	25.86853025697761
16-17	21.827236837864486	28.874840741498375	27.112695163606247	22.18522725703089
18-19	21.81776519130424	27.72077980592333	28.98384689609538	21.477608106677053
20-21	22.484045802198285	26.914329018448797	28.89748655557139	21.704138623781528
22-23	22.194942525030882	27.044064924107946	28.79241761090643	21.968574939954745
24-25	22.115681189754092	27.176208009947615	28.900126902520228	21.80798389777806
26-27	22.022982701912785	27.35912743528688	28.9372833832853	21.68060647951503
28-29	21.79682710788248	27.16214273045882	29.14692781346319	21.89410234819551
30-31	22.145330001748786	27.333088964439057	28.78905991418107	21.732521119631087
32-33	22.306735273611107	27.087987944289527	28.840390193747307	21.764886588352063
34-35	22.141750391332597	27.099939331869887	28.895057697574995	21.86325257922252
36-37	21.94282023357594	27.30461543518204	28.866176358659178	21.886387972582835
38-39	21.752563184493287	27.112011531628482	29.111770691301032	22.023654592577195
40-41	22.06399301047053	26.921146852310933	29.039965495577984	21.974894641640553
42-43	22.16482865956798	26.93889270311604	28.93621063992035	21.96006799739563
44-45	21.971612714915572	27.27444676584606	28.743801571360024	22.010138947878342
46-47	22.279201526823197	26.994303050808337	28.349573849885996	22.376921572482473
48-49	22.121019761231917	27.04927852513454	28.443452271363544	22.386249442269996
50-51	21.933227054769226	27.288051952938293	28.542187201701203	22.23653379059128
52-53	22.117023865642025	27.288867450860018	28.582624233986344	22.011484449511613
54-55	22.075365837630205	27.207374059923247	28.35458038111134	22.362679721335212
56-57	22.307877913706218	27.490214933133895	28.0195256156846	22.182381537475294
58-59	21.94356421380382	27.760712697356286	28.41773449950118	21.87798858933871
60-61	22.189368514429184	27.984777041677432	27.979042291251695	21.84681215264169
62-63	21.847616137387384	28.290284304455586	27.947762105647765	21.914337452509265
64-65	21.830573422040928	28.179504479726138	27.970336318837052	22.01958577939588
66-67	21.951375202470338	28.11207741014622	27.853173307928785	22.08337407945466
68-69	21.978782754944888	27.927174892332808	28.04152791770408	22.052514435018225
70-71	22.049162182428795	27.540982238263695	28.166815356134826	22.24304022317268
72-73	22.169345799200684	27.3856443902925	28.186782988824987	22.258226821681827
74-75	22.029288085041483	27.50823712261148	28.144944307125442	22.3175304852216
76-77	22.200920829817658	27.468726294846096	28.02677566136036	22.303577213975885
78-79	22.19152106243326	27.547582633763717	27.935076021366378	22.325820282436645
80-81	22.223065285887703	27.53463522942003	27.904584178595936	22.337715306096335
82-83	22.289978309247584	27.51505631852523	27.973045946785756	22.221919425441435
84-85	22.363844446992196	27.45799340313042	27.93760063472802	22.240561515149363
86-87	22.216333600078702	27.470998990926525	28.020978327288482	22.291689081706288
88-89	22.29525578820383	27.5385400474048	27.90470706276997	22.2614971016214
90-91	22.32909381877169	27.511912638804155	27.935533456616195	22.22346008580796
92-93	22.32476567490774	27.516299591814004	27.924844240374885	22.23409049290337
94-95	22.20546404117566	27.612706496342998	27.883267968964958	22.298561493516385
96-97	22.31182999397042	27.72205256885034	27.94852448475231	22.017592952426934
98-99	23.405778227562195	29.625430498151577	28.17064585921215	18.798145415074075
100	22.340843319086932	30.53667109012428	22.085476435072362	25.037009155716422
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	952.0
1	895.5
2	1169.0
3	1672.5
4	2220.5
5	3157.5
6	4049.0
7	5138.0
8	6449.5
9	7109.5
10	7063.5
11	7061.0
12	7937.0
13	10039.0
14	14008.5
15	18549.0
16	21844.5
17	23598.0
18	23400.5
19	21767.5
20	19748.0
21	18223.0
22	18589.5
23	21701.5
24	27290.0
25	35422.5
26	46610.5
27	59370.5
28	70962.5
29	83251.5
30	96047.0
31	108234.0
32	121587.5
33	135751.5
34	150622.5
35	164609.5
36	180165.0
37	194517.5
38	203686.5
39	212004.0
40	222977.5
41	232107.0
42	240295.0
43	256466.0
44	270370.5
45	274358.0
46	269909.0
47	263013.5
48	258948.5
49	253456.5
50	246412.0
51	237974.5
52	227708.5
53	215646.5
54	202452.5
55	188053.5
56	172626.5
57	155809.0
58	138525.0
59	120950.0
60	101451.5
61	82175.5
62	64794.5
63	49883.5
64	37445.5
65	27509.0
66	20053.0
67	14886.0
68	11152.0
69	8105.0
70	5748.0
71	4036.0
72	2803.0
73	1804.5
74	1155.5
75	741.0
76	477.0
77	290.0
78	172.0
79	109.5
80	75.0
81	52.0
82	32.0
83	20.5
84	16.0
85	13.0
86	10.0
87	7.5
88	4.5
89	2.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.010195175116932081
3	2.466574625064213E-4
4	0.001364837959202198
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	9.04507522789067E-5
26-27	7.401251880637544E-4
28-29	0.001554734425447893
30-31	9.303715681742116E-4
32-33	8.744075270319787E-4
34-35	0.0012900908124694223
36-37	0.0
38-39	2.086277070279498E-4
40-41	8.562234602206135E-4
42-43	4.1379267247490834E-4
44-45	0.0
46-47	0.0
48-49	0.0019620647317135785
50-51	0.004041023354328075
52-53	0.0
54-55	0.0039922740664349735
56-57	0.03965874963153675
58-59	0.05702525688725523
60-61	0.03060009000983923
62-63	0.0073926747253202025
64-65	0.0
66-67	0.0
68-69	4.688518727209697E-5
70-71	0.0035566596270097854
72-73	0.008052394502834702
74-75	0.0060712562135966704
76-77	0.007356483362575272
78-79	0.011172273198663232
80-81	0.011887775812079952
82-83	0.04326222072296559
84-85	0.025652419954357155
86-87	0.0012591505954349137
88-89	0.0016744689685240146
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	42.0
20-21	149.0
22-23	372.0
24-25	482.0
26-27	1344.0
28-29	3503.0
30-31	11006.0
32-33	14065.0
34-35	19353.0
36-37	30422.0
38-39	35618.0
40-41	34770.0
42-43	39506.0
44-45	47105.0
46-47	47469.0
48-49	44771.0
50-51	40419.0
52-53	39566.0
54-55	42478.0
56-57	43945.0
58-59	44143.0
60-61	44816.0
62-63	44224.0
64-65	49434.0
66-67	55649.0
68-69	57415.0
70-71	67265.0
72-73	47289.0
74-75	45208.0
76-77	46738.0
78-79	48553.0
80-81	48382.0
82-83	45433.0
84-85	44695.0
86-87	46443.0
88-89	48009.0
90-91	49348.0
92-93	48257.0
94-95	58162.0
96-97	186339.0
98-99	415527.0
100-101	4043594.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.80156088362455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.49393801150093	78.55015069904243
2	6.900329078212299	11.979186691989794
3	1.540704802172525	4.0120674506841345
4	0.5208207535319918	1.8083221738864956
5	0.22179372504619022	0.9626020764101382
6	0.11155686335562187	0.5809985919929517
7	0.06235234712598755	0.378859673870531
8	0.039662230233274864	0.2754194793899154
9	0.025464676810477035	0.19893363250917998
>10	0.08156765037343071	1.0526642953996475
>50	0.0014086695382407753	0.08038797518190077
>100	3.4535091621990183E-4	0.0572495228004871
>500	2.7920591407286523E-5	0.023799269984773507
>1k	2.7920591406496587E-5	0.039358466857615765
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.644383083376142E-5	0.0
5	0.0	0.0	0.0	1.644383083376142E-5	0.0
6	1.644383083376142E-5	0.0	0.0	1.644383083376142E-5	0.0
7	1.644383083376142E-5	0.0	0.0	1.644383083376142E-5	0.0
8	1.644383083376142E-5	1.644383083376142E-5	0.0	1.644383083376142E-5	0.0
9	1.644383083376142E-5	4.933149250128426E-5	0.0	3.288766166752284E-5	0.0
10-11	1.644383083376142E-5	4.933149250128426E-5	0.0	8.221915416880711E-5	0.0
12-13	1.644383083376142E-5	4.933149250128426E-5	0.0	8.221915416880711E-5	0.0
14-15	3.288766166752284E-5	1.1510681583632995E-4	0.0	8.221915416880711E-5	0.0
16-17	9.866298500256852E-5	1.1510681583632995E-4	0.0	8.221915416880711E-5	0.0
18-19	9.866298500256852E-5	1.3977256208697208E-4	0.0	1.1510681583632995E-4	0.0
20-21	9.866298500256852E-5	1.6443830833761422E-4	0.0	1.3155064667009136E-4	0.0
22-23	1.0688490041944924E-4	1.6443830833761422E-4	0.0	1.6443830833761422E-4	0.0
24-25	1.1510681583632995E-4	1.6443830833761422E-4	0.0	2.1376980083889847E-4	0.0
26-27	1.1510681583632995E-4	1.6443830833761422E-4	0.0	2.466574625064213E-4	0.0
28-29	1.1510681583632995E-4	1.7266022375449493E-4	0.0	5.015368404297233E-4	0.0
30-31	1.1510681583632995E-4	2.631012933401827E-4	0.0	0.0014141694517034822	0.0
32-33	1.1510681583632995E-4	2.631012933401827E-4	0.0	0.0034696483059236598	0.0
34-35	1.1510681583632995E-4	3.042108704245863E-4	0.0	0.006330874870998147	0.0
36-37	1.1510681583632995E-4	3.7820810917651266E-4	0.0	0.010614492803192998	0.0
38-39	1.1510681583632995E-4	3.946519400102741E-4	0.0	0.0164109431720939	0.0
40-41	1.1510681583632995E-4	4.4398343251155837E-4	0.0	0.023095360406017916	0.0
42-43	1.1510681583632995E-4	4.522053479284391E-4	0.0	0.029212465476177164	8.22191541688071E-6
44-45	1.1510681583632995E-4	4.8509300959596196E-4	0.0	0.03597910186426999	1.644383083376142E-5
46-47	1.1510681583632995E-4	5.097587558466041E-4	0.0	0.04338704765487951	1.644383083376142E-5
48-49	1.1510681583632995E-4	5.097587558466041E-4	0.0	0.05081965919173967	1.644383083376142E-5
50-51	1.1510681583632995E-4	6.330874870998148E-4	0.0	0.05798094751984277	1.644383083376142E-5
52-53	1.1510681583632995E-4	6.577532333504569E-4	0.0	0.06510934818627834	1.644383083376142E-5
54-55	1.1510681583632995E-4	6.577532333504569E-4	0.0	0.07154710795769595	2.466574625064213E-5
56-57	1.1510681583632995E-4	6.577532333504569E-4	0.0	0.07703112554075538	3.288766166752284E-5
58-59	1.1510681583632995E-4	6.577532333504569E-4	0.0	0.08259736227798362	3.288766166752284E-5
60-61	1.1510681583632995E-4	6.741970641842182E-4	0.0	0.08757162110519645	3.288766166752284E-5
62-63	1.1510681583632995E-4	6.82418979601099E-4	0.0	0.09303097294200524	3.288766166752284E-5
64-65	1.1510681583632995E-4	8.550792033555939E-4	0.0	0.09817789199297255	3.288766166752284E-5
66-67	1.1510681583632995E-4	8.550792033555939E-4	0.0	0.10310281932768411	4.1109577084403554E-5
68-69	1.1510681583632995E-4	8.879668650231167E-4	0.0	0.10803596857781253	4.933149250128426E-5
70-71	1.1510681583632995E-4	8.879668650231167E-4	0.0	0.11196604414708151	4.933149250128426E-5
72-73	1.1510681583632995E-4	8.879668650231167E-4	0.0	0.11538636096050389	4.933149250128426E-5
74-75	1.1510681583632995E-4	8.961887804399974E-4	0.0	0.1186257956347549	4.933149250128426E-5
76-77	1.1510681583632995E-4	9.619641037750431E-4	0.0	0.12136369346857617	4.933149250128426E-5
78-79	1.1510681583632995E-4	9.866298500256852E-4	0.0	0.12320540252195744	4.933149250128426E-5
80-81	1.1510681583632995E-4	9.866298500256852E-4	0.0	0.12385493383989102	6.577532333504568E-5
82-83	1.1510681583632995E-4	9.866298500256852E-4	0.0	0.12426602961073506	9.866298500256852E-5
84-85	1.1510681583632995E-4	9.866298500256852E-4	0.0	0.12445513366532332	9.866298500256852E-5
86-87	1.2332873125321065E-4	0.0010359613425269696	0.0	0.12451268707324148	9.866298500256852E-5
88	1.3155064667009136E-4	0.0011181804966957766	0.0	0.12451268707324148	9.866298500256852E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7870	0.0	49.374218	1
CTTATAC	6205	0.0	38.18133	1
GTATCAA	14245	0.0	37.35312	1
TCAACGC	16820	0.0	30.994331	4
ATCAACG	16920	0.0	30.786089	3
CAACGCA	17125	0.0	30.39229	5
TATACAC	8740	0.0	30.044868	3
TATCAAC	17805	0.0	29.547728	2
AACGCAG	17805	0.0	29.279678	6
TTATACA	9520	0.0	26.056095	2
ACGCAGA	20180	0.0	25.83373	7
CGCAGAG	20690	0.0	25.196941	8
GTGGTAT	3860	0.0	23.418703	1
ACATCTC	10270	0.0	23.23285	8
ACACATC	10375	0.0	23.162876	6
TACACAT	11610	0.0	22.359295	5
TGGTATC	3750	0.0	21.706512	2
GCAGAGT	24400	0.0	21.365767	9
CACATCT	11325	0.0	21.295504	7
GTACATG	17745	0.0	20.376715	1
>>END_MODULE