FastQCFastQC Report
Fri 27 May 2016
SRR2140118_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140118_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4510750
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61350.051.6098441
GTATCAA110600.036.6361851
TCAACGC124550.031.8793934
TATCAAC127100.031.6107982
ATCAACG125850.031.4820883
CAACGCA128250.030.8926095
AACGCAG134800.029.4867426
ACGCAGA156450.025.3515877
CGCAGAG157750.025.1697888
TGGTATC25600.024.9035742
GTGGTAT28650.024.044551
GATCAAC19050.022.0113411
CTTATAC45500.021.7228091
GCAGAGT184850.021.5492029
GAGTACT109950.020.35201512-13
TACATGG131400.020.0260432
GTACATG136200.020.0114121
CAGAGTA179300.019.69870810-11
ACATGGG133550.019.286723
TATACAC62150.019.2074133
Produced by FastQC (version 0.11.5)