##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140118_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4510750
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.308805852685254	25.0	18.0	33.0	18.0	33.0
2	29.28538203181289	30.0	27.0	33.0	25.0	33.0
3	28.208690794213823	29.0	27.0	31.0	18.0	33.0
4	31.604987418943633	33.0	31.0	33.0	29.0	33.0
5	32.13249370947182	33.0	33.0	33.0	31.0	33.0
6	36.48645546749432	38.0	37.0	38.0	34.0	38.0
7	36.65297034861165	38.0	37.0	38.0	34.0	38.0
8	36.911599844815164	38.0	38.0	38.0	35.0	38.0
9	37.280243861885495	38.0	38.0	38.0	36.0	38.0
10-11	37.28580457795267	38.0	38.0	38.0	36.5	38.0
12-13	37.396544920467775	38.0	38.0	38.0	37.0	38.0
14-15	37.34915712464668	38.0	38.0	38.0	36.5	38.0
16-17	37.43656753311534	38.0	38.0	38.0	37.0	38.0
18-19	37.45363354209389	38.0	38.0	38.0	37.0	38.0
20-21	37.472605804313105	38.0	38.0	38.0	37.0	38.0
22-23	37.49386262156027	38.0	38.0	38.0	37.0	38.0
24-25	37.501972334317344	38.0	38.0	38.0	37.0	38.0
26-27	37.46378189079462	38.0	38.0	38.0	37.0	38.0
28-29	37.43795578706688	38.0	38.0	38.0	37.0	38.0
30-31	37.44600340932021	38.0	38.0	38.0	37.0	38.0
32-33	37.435535926494794	38.0	38.0	38.0	37.0	38.0
34-35	37.40339616136171	38.0	38.0	38.0	37.0	38.0
36-37	37.37812211245251	38.0	38.0	38.0	37.0	38.0
38-39	37.35551380443701	38.0	38.0	38.0	37.0	38.0
40-41	37.36766531888561	38.0	38.0	38.0	37.0	38.0
42-43	37.33203121550149	38.0	38.0	38.0	37.0	38.0
44-45	37.28907698666691	38.0	38.0	38.0	37.0	38.0
46-47	37.25694565767933	38.0	38.0	38.0	37.0	38.0
48-49	37.2054630090215	38.0	38.0	38.0	37.0	38.0
50-51	37.17090708703297	38.0	38.0	38.0	37.0	38.0
52-53	37.137429703175016	38.0	38.0	38.0	36.5	38.0
54-55	37.119880949977684	38.0	38.0	38.0	36.0	38.0
56-57	37.07563663392011	38.0	38.0	38.0	36.0	38.0
58-59	37.042534786648964	38.0	38.0	38.0	36.0	38.0
60-61	37.06369440762893	38.0	38.0	38.0	36.0	38.0
62-63	37.07799485475108	38.0	38.0	38.0	36.0	38.0
64-65	37.08632149680133	38.0	38.0	38.0	36.0	38.0
66-67	37.078831402027824	38.0	38.0	38.0	36.0	38.0
68-69	37.03998728940489	38.0	38.0	38.0	36.0	38.0
70-71	37.03517662842246	38.0	38.0	38.0	36.0	38.0
72-73	37.05059303285192	38.0	38.0	38.0	36.0	38.0
74-75	37.06273348089007	38.0	38.0	38.0	36.0	38.0
76-77	37.043491692721894	38.0	38.0	38.0	36.0	38.0
78-79	37.004855262464034	38.0	38.0	38.0	36.0	38.0
80-81	36.95625218845362	38.0	38.0	38.0	36.0	38.0
82-83	36.91215054531434	38.0	38.0	38.0	35.5	38.0
84-85	36.88893222370159	38.0	38.0	38.0	35.0	38.0
86-87	36.91275140948602	38.0	38.0	38.0	35.0	38.0
88-89	36.86901762541344	38.0	38.0	38.0	35.0	38.0
90-91	36.88361894818594	38.0	38.0	38.0	35.0	38.0
92-93	36.8697122204406	38.0	38.0	38.0	35.0	38.0
94-95	36.88001498837549	38.0	38.0	38.0	35.0	38.0
96-97	36.87504387256389	38.0	38.0	38.0	35.0	38.0
98-99	36.86221956343043	38.0	38.0	38.0	35.0	38.0
100	35.30906692650919	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	4.0
20	24.0
21	65.0
22	269.0
23	720.0
24	1838.0
25	3912.0
26	6982.0
27	11556.0
28	18156.0
29	26625.0
30	36407.0
31	46865.0
32	60233.0
33	82582.0
34	128822.0
35	252895.0
36	849348.0
37	2983444.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.725943579227405	18.581499750595796	11.450113617469379	22.242443052707422
2	14.717394673288824	20.083835253329276	41.78546874823329	23.413301325148602
3	19.385094787024048	25.246899402847955	29.683019031893632	25.684986778234364
4	12.921401869946203	17.67162123635306	38.57139816765313	30.8355787260476
5	13.803979382586046	37.43446211827302	34.93095383251122	13.830604666629718
6	30.49206894640581	37.15637089175858	18.51853904561326	13.833021116222358
7	27.024175580557557	32.54993072105526	22.320501025328383	18.105392673058805
8	24.339721775757912	36.88242531729757	20.77477137948235	18.00308152746217
9	25.39327162888655	17.50292080031037	21.123604722052875	35.98020284875021
10-11	24.478190988194868	26.766480075375494	28.241002050656768	20.514326885772878
12-13	25.286116499473483	24.081327938812834	28.620174028709194	22.012381533004486
14-15	22.38582275674777	25.548013079864766	26.32501247021005	25.74115169317741
16-17	21.974394502022946	28.822036246743888	26.987862328881008	22.215706922352158
18-19	21.97143490550352	27.74040902288976	28.839184171146705	21.448971900460013
20-21	22.73553844238738	26.90264896191637	28.802280093861437	21.55953250183481
22-23	22.395539056369373	26.85197101756769	28.74934417286855	22.00314575319439
24-25	22.26143442765614	26.94368142375411	28.861682264955324	21.933201883634425
26-27	22.117824976065467	27.21825576992692	28.761020001895915	21.9028992521117
28-29	21.990248782484187	27.18191810175116	28.806363135251562	22.021469980513093
30-31	22.47772261893316	27.00217761108492	28.727690699733255	21.792409070248663
32-33	22.232421598139098	27.165574146607092	28.743246993205435	21.858757262048375
34-35	22.268966356051635	27.22513024741709	28.782586960863842	21.723316435667428
36-37	22.30967329025966	27.055359050849404	28.73126133910836	21.903706319782575
38-39	21.98388278256161	27.131611168325247	28.89043405262346	21.99407199648968
40-41	22.26350332010735	26.992835736923176	28.809287226150694	21.93437371681878
42-43	22.27107378801252	26.991329218111225	28.667432262834442	22.07016473104182
44-45	22.176936491229	27.206788918203074	28.5734891216668	22.04278546890113
46-47	22.355911910137387	27.076274627641432	28.225974490044525	22.341838972176653
48-49	22.254306885270918	27.177009787154923	28.283273418862358	22.285409908711806
50-51	22.14559518932843	27.412132669021815	28.23247663317155	22.209795508478205
52-53	22.30113152198763	27.38114565487837	28.226007488056815	22.09171533507718
54-55	22.288767866612698	27.290229659997024	28.09985394023567	22.321148533154613
56-57	22.358083326787714	27.512566385839488	27.909989933398517	22.21936035397428
58-59	22.167457357921464	27.631471900002552	28.145362525970167	22.055708216105817
60-61	22.310264611805756	27.742176539065174	27.986440933518576	21.9611179156105
62-63	22.088182830574585	27.8717908133437	27.963143923772154	22.076882432309567
64-65	22.04917914535204	27.805509110961758	27.976565061206536	22.16874668247966
66-67	22.200643764976093	27.888497557146312	27.758173546509585	22.152685131368006
68-69	22.121471024365743	27.758544469277467	27.900506178894812	22.219478327461974
70-71	22.114581858576436	27.521555878874892	27.991178212247736	22.372684050300933
72-73	22.304891189752706	27.40230491706154	27.985002127954072	22.307801765231687
74-75	22.106799800018162	27.511867398263412	27.9762954721231	22.405037329595327
76-77	22.38168896172513	27.372547946078075	27.80417813708062	22.441584955116173
78-79	22.343781792565448	27.530535472467736	27.705067525748824	22.420615209217992
80-81	22.336905991648976	27.496924841449182	27.66875833309313	22.49741083380871
82-83	22.417434123258673	27.427734554766893	27.826527739427508	22.328303582546926
84-85	22.55036456248406	27.411584518368475	27.73632119290109	22.301729726246368
86-87	22.335409608618413	27.494673883567632	27.795462254846804	22.37445425296715
88-89	22.456073124397875	27.52031113546453	27.689229373281222	22.334386366856375
90-91	22.503712116680248	27.47099746477948	27.714029821068532	22.311260597471744
92-93	22.52187350846324	27.49133889204483	27.70404222861921	22.28274537087272
94-95	22.396646993482385	27.643788056992285	27.695637786787547	22.263927162737787
96-97	22.48771828007194	27.664258685930516	27.81368294508189	22.034340088915652
98-99	23.54134586098195	29.629888072802185	27.96069267939108	18.868073386824783
100	22.528499482552686	30.44019246248424	21.90181329224773	25.129494762715343
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	663.0
1	667.0
2	911.0
3	1266.0
4	1613.0
5	2227.0
6	2869.0
7	3615.5
8	4918.0
9	5803.0
10	5617.5
11	5347.5
12	5819.0
13	7288.0
14	9878.5
15	12812.5
16	15369.5
17	17423.5
18	17976.0
19	16747.5
20	14946.0
21	13536.5
22	13317.0
23	14904.5
24	18527.5
25	24062.0
26	31694.0
27	40615.5
28	49079.0
29	58188.0
30	67614.5
31	76838.5
32	86978.0
33	97083.0
34	107455.5
35	118403.5
36	130031.5
37	140286.5
38	147619.5
39	154347.5
40	162700.5
41	170051.0
42	175285.0
43	180551.0
44	187762.0
45	196842.0
46	199342.0
47	197837.5
48	197300.5
49	194302.0
50	189541.0
51	183917.5
52	176502.5
53	166939.0
54	157133.0
55	146884.0
56	135263.0
57	121386.0
58	107890.5
59	95027.0
60	80566.5
61	65564.5
62	51517.5
63	39809.0
64	30083.0
65	22451.5
66	16638.5
67	12603.0
68	9609.5
69	6946.5
70	4913.5
71	3492.5
72	2397.0
73	1571.0
74	1020.5
75	688.5
76	468.0
77	283.5
78	180.5
79	121.5
80	81.0
81	51.5
82	36.0
83	22.0
84	11.5
85	6.5
86	6.0
87	3.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005254115169317741
3	1.3301557390677825E-4
4	7.09416394169484E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.108518163956931E-5
24-25	4.43437447272516E-5
26-27	7.539251505327977E-4
28-29	0.0013641758331703901
30-31	7.327484372474239E-4
32-33	5.674529673283948E-4
34-35	0.0010712030927418625
36-37	0.0
38-39	1.6906852933598222E-4
40-41	8.620634896148573E-4
42-43	3.881623719734894E-4
44-45	0.0
46-47	0.0
48-49	0.0015562971174803058
50-51	0.003573764745317955
52-53	0.0
54-55	0.003431658930886389
56-57	0.04013704639748634
58-59	0.05883310935167321
60-61	0.030696489772587827
62-63	0.006599687853268338
64-65	0.0
66-67	0.0
68-69	6.335309696546268E-5
70-71	0.003468891911760094
72-73	0.008136013449127732
74-75	0.006014227758152899
76-77	0.007014354026686656
78-79	0.009960124971680044
80-81	0.011166522203416232
82-83	0.04378134438492082
84-85	0.025551890812621103
86-87	8.676512350533303E-4
88-89	0.001779664964166863
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	35.0
20-21	128.0
22-23	306.0
24-25	375.0
26-27	1094.0
28-29	3028.0
30-31	9351.0
32-33	11859.0
34-35	16235.0
36-37	25209.0
38-39	28172.0
40-41	28132.0
42-43	31051.0
44-45	36488.0
46-47	37466.0
48-49	34626.0
50-51	30328.0
52-53	27867.0
54-55	29773.0
56-57	30835.0
58-59	32840.0
60-61	34085.0
62-63	32776.0
64-65	34848.0
66-67	38048.0
68-69	38540.0
70-71	43237.0
72-73	33376.0
74-75	32902.0
76-77	34057.0
78-79	35002.0
80-81	34796.0
82-83	32695.0
84-85	32369.0
86-87	33801.0
88-89	35316.0
90-91	35966.0
92-93	35239.0
94-95	41620.0
96-97	138550.0
98-99	309327.0
100-101	3009002.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.4059333700414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.75914479334766	81.12052840692672
2	6.142339788580971	10.860385641708868
3	1.220370937324208	3.236640953154567
4	0.4116016037345699	1.4555209581904225
5	0.1864670000549278	0.8242394591283725
6	0.09801578499463784	0.5199106174468954
7	0.05524389404260485	0.3418721611082505
8	0.03158084788566577	0.22335474671596645
9	0.02018033050478918	0.16056548585926256
>10	0.07306966998032735	0.9933644207614118
>50	0.0011242201684429681	0.0693444600574917
>100	8.256309152476411E-4	0.151749062544058
>500	0.0	0.0
>1k	3.5498465897766795E-5	0.042523626397649714
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.2169262317796375E-5	0.0	0.0	0.0
5	0.0	2.2169262317796375E-5	0.0	0.0	0.0
6	2.2169262317796375E-5	2.2169262317796375E-5	0.0	0.0	2.2169262317796375E-5
7	2.2169262317796375E-5	2.2169262317796375E-5	0.0	2.2169262317796375E-5	2.2169262317796375E-5
8	2.2169262317796375E-5	2.2169262317796375E-5	0.0	2.2169262317796375E-5	2.2169262317796375E-5
9	2.2169262317796375E-5	4.433852463559275E-5	0.0	2.2169262317796375E-5	4.433852463559275E-5
10-11	2.2169262317796375E-5	4.433852463559275E-5	0.0	2.2169262317796375E-5	4.433852463559275E-5
12-13	2.2169262317796375E-5	4.433852463559275E-5	0.0	2.2169262317796375E-5	4.433852463559275E-5
14-15	2.2169262317796375E-5	6.650778695338912E-5	0.0	2.2169262317796375E-5	6.650778695338912E-5
16-17	2.2169262317796375E-5	6.650778695338912E-5	0.0	2.2169262317796375E-5	1.1084631158898187E-4
18-19	2.2169262317796375E-5	7.75924181122873E-5	0.0	2.2169262317796375E-5	1.1084631158898187E-4
20-21	2.2169262317796375E-5	1.3301557390677825E-4	0.0	2.2169262317796375E-5	1.1084631158898187E-4
22-23	2.2169262317796375E-5	1.3301557390677825E-4	0.0	3.325389347669456E-5	1.1084631158898187E-4
24-25	2.2169262317796375E-5	1.4410020506567644E-4	0.0	6.650778695338912E-5	1.1084631158898187E-4
26-27	2.2169262317796375E-5	1.5518483622457464E-4	0.0	1.9952336086016738E-4	1.1084631158898187E-4
28-29	4.433852463559275E-5	1.6626946738347283E-4	0.0	6.872471318516877E-4	1.1084631158898187E-4
30-31	4.433852463559275E-5	1.9952336086016738E-4	0.0	0.002150418444826248	1.1084631158898187E-4
32-33	6.650778695338912E-5	1.9952336086016738E-4	0.0	0.005243030538158843	1.3301557390677825E-4
34-35	6.650778695338912E-5	2.660311478135565E-4	0.0	0.009011805132184227	1.3301557390677825E-4
36-37	6.650778695338912E-5	3.325389347669456E-4	0.0	0.015884276450701104	1.3301557390677825E-4
38-39	6.650778695338912E-5	3.657928282436402E-4	0.0	0.034960926675164886	1.3301557390677825E-4
40-41	6.650778695338912E-5	4.2121598403813116E-4	0.0	0.0556891869423045	1.5518483622457464E-4
42-43	6.650778695338912E-5	4.2121598403813116E-4	0.0	0.06631934822368785	1.5518483622457464E-4
44-45	6.650778695338912E-5	4.544698775148257E-4	0.0	0.07868979659701823	1.5518483622457464E-4
46-47	6.650778695338912E-5	4.8772377099152027E-4	0.0	0.09253450091448207	1.77354098542371E-4
48-49	6.650778695338912E-5	5.431469267860112E-4	0.0	0.10544809621459847	1.77354098542371E-4
50-51	6.650778695338912E-5	5.653161891038077E-4	0.0	0.11778529069445215	1.77354098542371E-4
52-53	6.650778695338912E-5	5.764008202627058E-4	0.0	0.15569472925788394	1.77354098542371E-4
54-55	6.650778695338912E-5	5.764008202627058E-4	0.0	0.20668403258881562	1.77354098542371E-4
56-57	6.650778695338912E-5	5.985700825805021E-4	0.0	0.27667239372609875	1.77354098542371E-4
58-59	6.650778695338912E-5	5.985700825805021E-4	0.0	0.32034584049215764	1.884387297012692E-4
60-61	6.650778695338912E-5	5.985700825805021E-4	0.0	0.33674000997616804	1.9952336086016738E-4
62-63	8.86770492711855E-5	5.985700825805021E-4	0.0	0.3504406140885662	1.9952336086016738E-4
64-65	8.86770492711855E-5	6.429086072160949E-4	0.0	0.35691403868536276	1.9952336086016738E-4
66-67	8.86770492711855E-5	6.429086072160949E-4	0.0	0.3622124923793161	1.9952336086016738E-4
68-69	8.86770492711855E-5	6.650778695338912E-4	0.0	0.36796541595078425	1.9952336086016738E-4
70-71	8.86770492711855E-5	6.650778695338912E-4	0.0	0.3726209610375215	2.2169262317796375E-4
72-73	1.1084631158898187E-4	6.650778695338912E-4	0.0	0.37578008091780746	2.2169262317796375E-4
74-75	1.1084631158898187E-4	6.650778695338912E-4	0.0	0.3785844926010087	2.2169262317796375E-4
76-77	1.1084631158898187E-4	6.872471318516875E-4	0.0	0.3815440891204345	2.2169262317796375E-4
78-79	1.1084631158898187E-4	7.09416394169484E-4	0.0	0.38329546084354044	2.2169262317796375E-4
80-81	1.1084631158898187E-4	7.09416394169484E-4	0.0	0.38384969240148537	2.3277725433686194E-4
82-83	1.1084631158898187E-4	7.09416394169484E-4	0.0	0.38432633154131796	2.4386188549576013E-4
84-85	1.1084631158898187E-4	7.09416394169484E-4	0.0	0.3844815163775425	2.4386188549576013E-4
86-87	1.1084631158898187E-4	7.537549188050768E-4	0.0	0.3846145319514493	2.4386188549576013E-4
88	1.1084631158898187E-4	8.202627057584659E-4	0.0	0.3846367012137671	2.4386188549576013E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6135	0.0	51.609844	1
GTATCAA	11060	0.0	36.636185	1
TCAACGC	12455	0.0	31.879393	4
TATCAAC	12710	0.0	31.610798	2
ATCAACG	12585	0.0	31.482088	3
CAACGCA	12825	0.0	30.892609	5
AACGCAG	13480	0.0	29.486742	6
ACGCAGA	15645	0.0	25.351587	7
CGCAGAG	15775	0.0	25.169788	8
TGGTATC	2560	0.0	24.903574	2
GTGGTAT	2865	0.0	24.04455	1
GATCAAC	1905	0.0	22.011341	1
CTTATAC	4550	0.0	21.722809	1
GCAGAGT	18485	0.0	21.549202	9
GAGTACT	10995	0.0	20.352015	12-13
TACATGG	13140	0.0	20.026043	2
GTACATG	13620	0.0	20.011412	1
CAGAGTA	17930	0.0	19.698708	10-11
ACATGGG	13355	0.0	19.28672	3
TATACAC	6215	0.0	19.207413	3
>>END_MODULE