FastQCFastQC Report
Fri 27 May 2016
SRR2140117_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140117_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4666887
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC82850.051.0471231
GGTATCA52250.045.389161
TATACAC108050.042.7650153
TTATACA108500.041.6581542
GTATCAA97900.040.8332181
GCTCCGA15950.040.1432881
TCAACGC115750.033.80664
TATCAAC117300.033.6130452
ATCAACG116700.033.6035843
CAACGCA119600.032.717995
AACGCAG124950.031.3508136
TACACAT160050.028.8704195
ACGCAGA143750.027.2506737
ATACACA176900.027.0255664
CGCAGAG147850.026.4949878
ACACATC175000.025.8023266
CACATCT191650.023.7145427
GCAGAGT176600.022.1339769
CATCTCC207700.021.7806059
GATCAAC20400.021.4750021
Produced by FastQC (version 0.11.5)