##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140117_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4666887
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.08238296748989	25.0	18.0	33.0	18.0	33.0
2	29.091323616792092	30.0	27.0	32.0	25.0	33.0
3	27.955450817643538	29.0	25.0	31.0	18.0	33.0
4	31.569679960110456	33.0	31.0	33.0	29.0	33.0
5	32.156405758270985	33.0	33.0	33.0	31.0	33.0
6	36.499778117618874	38.0	37.0	38.0	34.0	38.0
7	36.72666983366	38.0	37.0	38.0	34.0	38.0
8	36.9650666493532	38.0	38.0	38.0	35.0	38.0
9	37.338223316741974	38.0	38.0	38.0	36.0	38.0
10-11	37.33680459801148	38.0	38.0	38.0	36.5	38.0
12-13	37.44362494742212	38.0	38.0	38.0	37.0	38.0
14-15	37.38730721356656	38.0	38.0	38.0	37.0	38.0
16-17	37.46899228543566	38.0	38.0	38.0	37.0	38.0
18-19	37.48651145828043	38.0	38.0	38.0	37.0	38.0
20-21	37.506963992133876	38.0	38.0	38.0	37.0	38.0
22-23	37.52167913057632	38.0	38.0	38.0	37.5	38.0
24-25	37.52726775366048	38.0	38.0	38.0	37.5	38.0
26-27	37.49377275341398	38.0	38.0	38.0	37.0	38.0
28-29	37.46410763283281	38.0	38.0	38.0	37.0	38.0
30-31	37.474054549277355	38.0	38.0	38.0	37.0	38.0
32-33	37.459980053727236	38.0	38.0	38.0	37.0	38.0
34-35	37.4159925302809	38.0	38.0	38.0	37.0	38.0
36-37	37.39827938698211	38.0	38.0	38.0	37.0	38.0
38-39	37.35188185234919	38.0	38.0	38.0	37.0	38.0
40-41	37.41303517098781	38.0	38.0	38.0	37.0	38.0
42-43	37.385279004763305	38.0	38.0	38.0	37.0	38.0
44-45	37.34750311844333	38.0	38.0	38.0	37.0	38.0
46-47	37.319730055271265	38.0	38.0	38.0	37.0	38.0
48-49	37.273625237119404	38.0	38.0	38.0	37.0	38.0
50-51	37.23299255406398	38.0	38.0	38.0	37.0	38.0
52-53	37.194988408879865	38.0	38.0	38.0	37.0	38.0
54-55	37.164012889606454	38.0	38.0	38.0	36.0	38.0
56-57	37.1091338723055	38.0	38.0	38.0	36.0	38.0
58-59	37.06192082830519	38.0	38.0	38.0	36.0	38.0
60-61	37.063096313946005	38.0	38.0	38.0	36.0	38.0
62-63	37.072448196836646	38.0	38.0	38.0	36.0	38.0
64-65	37.07739978762304	38.0	38.0	38.0	36.0	38.0
66-67	37.06386888663869	38.0	38.0	38.0	36.0	38.0
68-69	37.01475545683701	38.0	38.0	38.0	36.0	38.0
70-71	37.0110094338927	38.0	38.0	38.0	36.0	38.0
72-73	37.01920287536326	38.0	38.0	38.0	36.0	38.0
74-75	37.036280157953	38.0	38.0	38.0	36.0	38.0
76-77	37.02397073185777	38.0	38.0	38.0	36.0	38.0
78-79	36.99244743130455	38.0	38.0	38.0	36.0	38.0
80-81	36.95289253861509	38.0	38.0	38.0	36.0	38.0
82-83	36.91509410929609	38.0	38.0	38.0	35.5	38.0
84-85	36.89371272617545	38.0	38.0	38.0	35.0	38.0
86-87	36.91806786509207	38.0	38.0	38.0	35.0	38.0
88-89	36.880410421991115	38.0	38.0	38.0	35.0	38.0
90-91	36.89027215266796	38.0	38.0	38.0	35.0	38.0
92-93	36.88009359843857	38.0	38.0	38.0	35.0	38.0
94-95	36.89389916645037	38.0	38.0	38.0	35.0	38.0
96-97	36.891659052540064	38.0	38.0	38.0	35.0	38.0
98-99	36.87920350901746	38.0	38.0	38.0	35.0	38.0
100	35.38265417518509	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	8.0
20	45.0
21	99.0
22	348.0
23	871.0
24	1986.0
25	3818.0
26	6825.0
27	11462.0
28	17533.0
29	25666.0
30	34257.0
31	44119.0
32	57676.0
33	80406.0
34	127939.0
35	264715.0
36	947583.0
37	3041526.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.70404490187999	19.004017024624765	11.565803928828789	22.726134144666453
2	14.17012489923161	19.941641120103064	42.29572356311026	23.592510417555065
3	18.852710527251837	25.893241691769102	30.336940455696503	24.917107325282554
4	12.600999560303178	18.377055882637805	37.69832426655056	31.323620290508465
5	13.603093453944782	37.782466127849254	34.96971321568317	13.644727202522795
6	29.98529855126126	37.141010699423404	18.576022946345176	14.29766780297016
7	26.673283497114888	32.75946042833263	21.595980361212945	18.971275713339534
8	24.433953511194936	36.749914879018924	21.18720680402161	17.62892480576453
9	25.096065107211725	17.51396594775061	20.315040839857488	37.07492810518018
10-11	24.340904333016848	26.576998757415808	28.25781939866982	20.82427751089752
12-13	25.377773235135116	23.77732737047201	28.24800557630815	22.59689381808473
14-15	22.594493931393668	25.43487768184659	25.387512061037686	26.58311632572205
16-17	22.510594321225263	29.18355426218805	25.876113992046516	22.429737424540168
18-19	22.524907931132677	27.539149758714963	27.644851910920494	22.291090399231862
20-21	22.983177976220894	26.579303267733106	27.690014749845638	22.747504006200355
22-23	22.486754459555165	27.15054541090908	27.778809921003543	22.583890208532218
24-25	22.420348082426454	27.31941729388565	28.190609137817308	22.06962548587059
26-27	22.22776969589807	27.278331900027595	28.31680611258014	22.1770922914942
28-29	21.527387519240744	26.558090160424758	28.536796534940162	23.377725785394336
30-31	21.948915954781043	26.44120080175944	28.82410189505606	22.785781348403457
32-33	21.81492543093225	26.97630215518563	28.800446193505774	22.40832622037635
34-35	21.8831448674626	27.589688269449113	28.998238947575654	21.528927915512632
36-37	22.315786509064438	27.044752417761448	28.050094890637457	22.589366182536658
38-39	22.3333900496353	26.692254039183517	28.528107323956647	22.44624858722453
40-41	22.469488882603688	26.549300976331615	28.767479158688978	22.213730982375722
42-43	22.83721909826798	26.374728943806836	28.784808565596702	22.003243392328482
44-45	22.213981896003297	26.940521659419076	28.643117818736652	22.202378625840975
46-47	23.00679203063912	26.26837086183472	27.869623116156472	22.855213991369688
48-49	22.640378453474625	26.394030228831937	28.18146996823676	22.784121349456676
50-51	22.247611089110812	26.840565619534868	28.486175390759787	22.425647900594534
52-53	22.379459419528246	27.132756105691413	28.802981973325036	21.684802501455305
54-55	22.30996235287736	27.280400187719835	28.080736219670687	22.328901239732115
56-57	22.828772725148387	28.063922512501755	27.168060943122867	21.939243819226995
58-59	21.890062333579895	28.94922569260744	27.961145445059028	21.199566528753635
60-61	22.5556391209073	29.695481012301393	26.65754676999959	21.09133309679171
62-63	21.858987226123652	30.251142871308623	26.625712474421682	21.264157428146042
64-65	21.951419559050606	29.989083170035197	26.707499415582703	21.351997855331497
66-67	22.25491267978689	29.661600701513468	26.66318838662514	21.420298232074497
68-69	22.196407368261056	29.104478173475485	27.066322576373146	21.632791881890313
70-71	22.358829492168113	28.40110301837488	27.311619785239017	21.92844770421799
72-73	22.657294528908533	27.802772165114455	27.480612504609887	22.059320801367125
74-75	22.459568729619143	27.73981422570177	27.584608920921877	22.216008123757216
76-77	22.546793868672665	27.558179560300477	27.55558468174344	22.339441889283414
78-79	22.52797575022669	27.69923883814937	27.466307612835383	22.306477798788553
80-81	22.579618836888738	27.641708782974373	27.458729305884432	22.31994307425246
82-83	22.584666627437073	27.48928479222129	27.624176332187073	22.30187224815456
84-85	22.666042324241037	27.525905753700496	27.54524529792685	22.262806624131613
86-87	22.45056971348973	27.49435464205424	27.642698392019444	22.41237725243658
88-89	22.56269536490522	27.49521449147046	27.600641930336046	22.341448213288274
90-91	22.604228351567187	27.454861222655257	27.61508678932243	22.325823636455127
92-93	22.59796688099429	27.476303028126008	27.594223930457275	22.33150616042243
94-95	22.482095522990882	27.554537919572052	27.622719853320945	22.340646704116125
96-97	22.564111646358743	27.671584254274112	27.670757768961934	22.093546330405214
98-99	23.674822098705373	29.630636946009687	27.806645904599193	18.88789505068575
100	30.245619290100343	40.71935487945492	29.035025830444738	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	684.0
1	674.5
2	743.0
3	931.0
4	1275.0
5	1884.5
6	2400.0
7	2852.0
8	3614.0
9	4117.0
10	4095.5
11	4110.5
12	4578.0
13	5866.5
14	8067.0
15	10535.0
16	12937.0
17	15012.0
18	15551.0
19	14444.0
20	12986.0
21	11926.5
22	12006.0
23	13762.0
24	17346.5
25	22961.5
26	30623.0
27	39222.0
28	47770.0
29	57237.0
30	67072.5
31	76935.0
32	87473.5
33	98905.5
34	110520.5
35	121253.0
36	133274.5
37	143590.0
38	151918.5
39	160691.0
40	170710.0
41	179618.0
42	188499.5
43	211989.5
44	241601.5
45	256551.0
46	249568.0
47	232339.5
48	215444.5
49	202134.5
50	195291.0
51	188445.5
52	179512.5
53	169567.0
54	158740.5
55	146415.0
56	133442.0
57	120159.0
58	106825.5
59	92314.5
60	77397.5
61	63104.5
62	49973.5
63	38988.5
64	29825.0
65	22427.5
66	16504.0
67	12452.0
68	9517.0
69	7061.0
70	5158.0
71	3675.0
72	2607.0
73	1752.0
74	1149.0
75	773.5
76	534.5
77	356.0
78	224.0
79	136.5
80	84.0
81	55.5
82	41.5
83	29.0
84	19.0
85	15.0
86	13.0
87	7.0
88	1.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005249752136702688
3	1.9284803767479264E-4
4	7.499645909575269E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.2144917861698636E-5
26-27	6.644064701616661E-4
28-29	0.0013400533255460154
30-31	7.834651504505301E-4
32-33	6.669193071741047E-4
34-35	0.0011434574117668889
36-37	0.0
38-39	2.6159917099222716E-4
40-41	7.024155962602955E-4
42-43	2.8729884936810825E-4
44-45	0.0
46-47	0.0
48-49	0.0016993275534294072
50-51	0.004191747670953062
52-53	0.0
54-55	0.003697234723408595
56-57	0.04090021585316659
58-59	0.06004391682716641
60-61	0.033127052233370924
62-63	0.007195828825912684
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.004196865923400436
72-73	0.008258157267515992
74-75	0.005939829916313959
76-77	0.007678132678132678
78-79	0.01137849002757579
80-81	0.012535710903812598
82-83	0.04728524040007271
84-85	0.027630129988392593
86-87	0.0012944070342253747
88-89	0.0015749882860246229
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	38.0
20-21	130.0
22-23	293.0
24-25	414.0
26-27	1326.0
28-29	3448.0
30-31	10217.0
32-33	12337.0
34-35	16689.0
36-37	26867.0
38-39	31890.0
40-41	30537.0
42-43	33247.0
44-45	38583.0
46-47	38052.0
48-49	36614.0
50-51	33230.0
52-53	30858.0
54-55	32741.0
56-57	33567.0
58-59	37854.0
60-61	51082.0
62-63	46233.0
64-65	54336.0
66-67	63357.0
68-69	61639.0
70-71	79999.0
72-73	39255.0
74-75	36011.0
76-77	37042.0
78-79	37956.0
80-81	37992.0
82-83	35925.0
84-85	35570.0
86-87	36638.0
88-89	37797.0
90-91	37964.0
92-93	37438.0
94-95	43985.0
96-97	139908.0
98-99	1051064.0
100-101	2216764.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.67831656646952
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.10270482884665	81.67512815440564
2	5.8384801027761375	10.354931736420317
3	1.1636592544266136	3.095740312186354
4	0.4153231215518484	1.4732062100139647
5	0.18544937406574724	0.8222669150227978
6	0.10098454157134906	0.5373083487470337
7	0.05531688818844633	0.34337859655729297
8	0.03420836291517881	0.24268320286503214
9	0.02258803851865771	0.18027623073358218
>10	0.07929778895653296	1.049403199812323
>50	0.0011176931225757416	0.0653801856913881
>100	8.01119418175513E-4	0.11936886771955595
>500	6.888564203218466E-5	0.04092803982472144
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1427559741643626E-5
2	0.0	0.0	0.0	0.0	2.1427559741643626E-5
3	0.0	0.0	0.0	2.1427559741643626E-5	2.1427559741643626E-5
4	0.0	4.285511948328725E-5	0.0	2.1427559741643626E-5	2.1427559741643626E-5
5	0.0	6.428267922493088E-5	0.0	2.1427559741643626E-5	2.1427559741643626E-5
6	0.0	6.428267922493088E-5	0.0	2.1427559741643626E-5	2.1427559741643626E-5
7	0.0	6.428267922493088E-5	0.0	2.1427559741643626E-5	2.1427559741643626E-5
8	0.0	6.428267922493088E-5	0.0	2.1427559741643626E-5	2.1427559741643626E-5
9	0.0	8.57102389665745E-5	0.0	6.428267922493088E-5	2.1427559741643626E-5
10-11	0.0	8.57102389665745E-5	0.0	6.428267922493088E-5	2.1427559741643626E-5
12-13	0.0	8.57102389665745E-5	0.0	6.428267922493088E-5	2.1427559741643626E-5
14-15	0.0	1.1785157857903994E-4	0.0	7.499645909575269E-5	8.57102389665745E-5
16-17	3.214133961246544E-5	1.4999291819150538E-4	0.0	8.57102389665745E-5	1.1785157857903994E-4
18-19	4.285511948328725E-5	1.607066980623272E-4	0.0	8.57102389665745E-5	1.2856535844986175E-4
20-21	4.285511948328725E-5	1.71420477933149E-4	0.0	8.57102389665745E-5	1.2856535844986175E-4
22-23	4.285511948328725E-5	2.2498937728725809E-4	0.0	9.642401883739632E-5	1.2856535844986175E-4
24-25	4.285511948328725E-5	2.7855827664136713E-4	0.0	1.2856535844986175E-4	1.2856535844986175E-4
26-27	4.285511948328725E-5	3.214133961246544E-4	0.0	2.3570315715807987E-4	1.2856535844986175E-4
28-29	4.285511948328725E-5	3.214133961246544E-4	0.0	9.963815279864286E-4	1.2856535844986175E-4
30-31	4.285511948328725E-5	3.856960753495853E-4	0.0	0.0021427559741643626	1.2856535844986175E-4
32-33	4.285511948328725E-5	3.856960753495853E-4	0.0	0.005003335199673787	1.2856535844986175E-4
34-35	4.285511948328725E-5	4.821200941869816E-4	0.0	0.008624592796011559	1.4999291819150538E-4
36-37	4.285511948328725E-5	5.571165532827343E-4	0.0	0.014517171724963557	1.4999291819150538E-4
38-39	4.285511948328725E-5	5.571165532827343E-4	0.0	0.02994501473894697	1.4999291819150538E-4
40-41	4.285511948328725E-5	5.999716727660215E-4	0.0	0.04733347946929077	1.607066980623272E-4
42-43	4.285511948328725E-5	6.32113012378487E-4	0.0	0.05819725225830409	1.8213425780397083E-4
44-45	4.285511948328725E-5	6.963956916034178E-4	0.0	0.07006812035517465	1.9284803767479264E-4
46-47	4.285511948328725E-5	7.071094714742397E-4	0.0	0.08234611208713646	2.0356181754561446E-4
48-49	4.285511948328725E-5	7.178232513450615E-4	0.0	0.09420626640413621	2.1427559741643627E-4
50-51	4.285511948328725E-5	9.106712890198541E-4	0.0	0.10662353727441869	2.1427559741643627E-4
52-53	4.285511948328725E-5	9.21385068890676E-4	0.0	0.13392224838527267	2.1427559741643627E-4
54-55	4.285511948328725E-5	9.642401883739632E-4	0.0	0.16874203296544354	2.1427559741643627E-4
56-57	4.285511948328725E-5	0.0010070953078572505	0.0	0.20954010671353301	2.1427559741643627E-4
58-59	4.285511948328725E-5	0.0010178090877280724	0.0	0.24261354517475997	2.1427559741643627E-4
60-61	6.428267922493088E-5	0.001028522867598894	0.0	0.2545701235105971	2.1427559741643627E-4
62-63	6.428267922493088E-5	0.0010392366474697159	0.0	0.26858374758163206	2.571307168997235E-4
64-65	6.428267922493088E-5	0.0011785157857903995	0.0	0.27580483521456595	2.571307168997235E-4
66-67	6.428267922493088E-5	0.0011785157857903995	0.0	0.28184740706170947	2.571307168997235E-4
68-69	6.428267922493088E-5	0.0011892295656612214	0.0	0.2877185584309198	2.571307168997235E-4
70-71	6.428267922493088E-5	0.001199943345532043	0.0	0.29238976645459813	2.571307168997235E-4
72-73	6.428267922493088E-5	0.001199943345532043	0.0	0.29609673428990246	2.7855827664136713E-4
74-75	6.428267922493088E-5	0.001210657125402865	0.0	0.2987001827985122	2.7855827664136713E-4
76-77	7.499645909575269E-5	0.0012320846851445084	0.0	0.3016678998227298	2.7855827664136713E-4
78-79	8.57102389665745E-5	0.0012535122448861522	0.0	0.3036285215390902	2.7855827664136713E-4
80-81	8.57102389665745E-5	0.001264226024756974	0.0	0.304303489670952	2.8927205651218895E-4
82-83	8.57102389665745E-5	0.001264226024756974	0.0	0.3049356026833304	3.321271759954762E-4
84-85	8.57102389665745E-5	0.0012856535844986176	0.0	0.30509630938139276	3.42840955866298E-4
86-87	8.57102389665745E-5	0.0013285087039819048	0.0	0.30516059206061774	3.42840955866298E-4
88	8.57102389665745E-5	0.0013927913832068358	0.0	0.3051713058404885	3.42840955866298E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	8285	0.0	51.047123	1
GGTATCA	5225	0.0	45.38916	1
TATACAC	10805	0.0	42.765015	3
TTATACA	10850	0.0	41.658154	2
GTATCAA	9790	0.0	40.833218	1
GCTCCGA	1595	0.0	40.143288	1
TCAACGC	11575	0.0	33.8066	4
TATCAAC	11730	0.0	33.613045	2
ATCAACG	11670	0.0	33.603584	3
CAACGCA	11960	0.0	32.71799	5
AACGCAG	12495	0.0	31.350813	6
TACACAT	16005	0.0	28.870419	5
ACGCAGA	14375	0.0	27.250673	7
ATACACA	17690	0.0	27.025566	4
CGCAGAG	14785	0.0	26.494987	8
ACACATC	17500	0.0	25.802326	6
CACATCT	19165	0.0	23.714542	7
GCAGAGT	17660	0.0	22.133976	9
CATCTCC	20770	0.0	21.780605	9
GATCAAC	2040	0.0	21.475002	1
>>END_MODULE