FastQCFastQC Report
Fri 27 May 2016
SRR2140116_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140116_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4937470
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67700.051.736771
GTATCAA123800.038.5270651
TCAACGC143800.032.573974
TATCAAC146050.032.2487072
ATCAACG146100.032.061173
CAACGCA147750.031.7319975
AACGCAG153850.030.5847726
ACGCAGA175450.026.6978427
CGCAGAG180450.025.9344448
GATCAAC21200.025.3559271
GTGGTAT32550.022.8056811
TGGTATC28850.022.772972
GCAGAGT209900.022.2957139
GAGTACT124000.020.98627512-13
TACATGG155450.020.665652
GTACATG156600.020.5683751
ACATGGG153400.020.19013
CAGAGTA202850.019.9475510-11
GTACTTT137550.018.85708414-15
AGAGTAC190450.018.32312610-11
Produced by FastQC (version 0.11.5)