##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140116_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4937470
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.3135449936911	25.0	18.0	33.0	18.0	33.0
2	29.256570267768716	30.0	27.0	32.0	25.0	33.0
3	28.1658254126101	29.0	27.0	31.0	18.0	33.0
4	31.604328532629058	33.0	31.0	33.0	29.0	33.0
5	32.13806585153935	33.0	33.0	33.0	31.0	33.0
6	36.51771798107128	38.0	37.0	38.0	34.0	38.0
7	36.70093610695356	38.0	37.0	38.0	34.0	38.0
8	36.959021118102996	38.0	38.0	38.0	35.0	38.0
9	37.3079226810492	38.0	38.0	38.0	36.0	38.0
10-11	37.314764545404834	38.0	38.0	38.0	36.5	38.0
12-13	37.41939363682209	38.0	38.0	38.0	37.0	38.0
14-15	37.37132337006605	38.0	38.0	38.0	37.0	38.0
16-17	37.453070094603106	38.0	38.0	38.0	37.0	38.0
18-19	37.46907535640723	38.0	38.0	38.0	37.0	38.0
20-21	37.48736819361234	38.0	38.0	38.0	37.0	38.0
22-23	37.50588027290022	38.0	38.0	38.0	37.0	38.0
24-25	37.51392226259985	38.0	38.0	38.0	37.0	38.0
26-27	37.47907983387603	38.0	38.0	38.0	37.0	38.0
28-29	37.45850808400537	38.0	38.0	38.0	37.0	38.0
30-31	37.46365114074439	38.0	38.0	38.0	37.0	38.0
32-33	37.45641688313376	38.0	38.0	38.0	37.0	38.0
34-35	37.42607773471626	38.0	38.0	38.0	37.0	38.0
36-37	37.40094879695701	38.0	38.0	38.0	37.0	38.0
38-39	37.39382847685135	38.0	38.0	38.0	37.0	38.0
40-41	37.3831774804185	38.0	38.0	38.0	37.0	38.0
42-43	37.342371911313116	38.0	38.0	38.0	37.0	38.0
44-45	37.299534578292494	38.0	38.0	38.0	37.0	38.0
46-47	37.26550307301342	38.0	38.0	38.0	37.0	38.0
48-49	37.217662462650495	38.0	38.0	38.0	37.0	38.0
50-51	37.18402565072537	38.0	38.0	38.0	37.0	38.0
52-53	37.15119988512721	38.0	38.0	38.0	37.0	38.0
54-55	37.136263771358756	38.0	38.0	38.0	36.0	38.0
56-57	37.09373689076816	38.0	38.0	38.0	36.0	38.0
58-59	37.0670060454336	38.0	38.0	38.0	36.0	38.0
60-61	37.091014120774844	38.0	38.0	38.0	36.0	38.0
62-63	37.10553602058956	38.0	38.0	38.0	36.0	38.0
64-65	37.114775453790834	38.0	38.0	38.0	36.0	38.0
66-67	37.111357615090455	38.0	38.0	38.0	36.0	38.0
68-69	37.076101722789716	38.0	38.0	38.0	36.0	38.0
70-71	37.07301507642309	38.0	38.0	38.0	36.0	38.0
72-73	37.085204361517256	38.0	38.0	38.0	36.0	38.0
74-75	37.09801128237989	38.0	38.0	38.0	36.0	38.0
76-77	37.080425230096274	38.0	38.0	38.0	36.0	38.0
78-79	37.04298969153821	38.0	38.0	38.0	36.0	38.0
80-81	36.995588892049156	38.0	38.0	38.0	36.0	38.0
82-83	36.955383834462	38.0	38.0	38.0	36.0	38.0
84-85	36.928976106508074	38.0	38.0	38.0	35.5	38.0
86-87	36.95325747034737	38.0	38.0	38.0	36.0	38.0
88-89	36.90811753760369	38.0	38.0	38.0	35.0	38.0
90-91	36.92006517576292	38.0	38.0	38.0	35.0	38.0
92-93	36.90838068871799	38.0	38.0	38.0	35.0	38.0
94-95	36.923835983326825	38.0	38.0	38.0	35.0	38.0
96-97	36.919622261847344	38.0	38.0	38.0	35.0	38.0
98-99	36.90517251814481	38.0	38.0	38.0	35.0	38.0
100	35.37267624439791	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	14.0
21	58.0
22	254.0
23	836.0
24	2000.0
25	3927.0
26	7310.0
27	12224.0
28	19376.0
29	28556.0
30	38126.0
31	49395.0
32	63866.0
33	87357.0
34	135991.0
35	269192.0
36	920501.0
37	3298481.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.00229672281553	18.50609725223647	11.556485406493609	21.93512061845439
2	14.824924010557403	20.34959498290427	41.38678631345425	23.438694693084074
3	19.192872121646403	25.544703367603326	29.819230867381556	25.443193643368712
4	12.972175644975318	17.655131599653828	38.88252143774189	30.490171317628967
5	13.816104199114122	37.607438627475204	34.866702987562455	13.709754185848219
6	30.462888888438815	37.32857111030548	18.59284208309114	13.615697918164566
7	27.09420006602572	32.74314578113892	22.60212213947629	17.56053201335907
8	24.6149546225091	36.5851336818249	20.80306310721888	17.99684858844712
9	25.947519681132242	17.26392261623868	21.119156167024812	35.669401535604266
10-11	24.511521082659744	26.728668731151785	28.42557018067958	20.334240005508892
12-13	25.421724081361507	24.199914126060513	28.688822413098208	21.689539379479776
14-15	22.58165619234142	25.559547703580982	26.534135903610554	25.324660200467044
16-17	22.000761523614322	28.690614829052123	27.171010659305274	22.137612988028284
18-19	21.985146238863223	27.703672123577462	29.059346183369218	21.2518354541901
20-21	22.86700341473887	26.887990083889967	29.007700377930174	21.237306123440987
22-23	22.566760859149678	26.666680844655723	28.900250859262087	21.86630743693251
24-25	22.299176900592347	26.80980135565144	28.96694952701484	21.924072216741376
26-27	22.146047008471257	27.01247427086042	28.91663064933202	21.924848071336303
28-29	22.08819604254763	27.111659659501424	28.957396637979343	21.842747659971607
30-31	22.455411481674084	26.88491401253143	28.822536043438184	21.8371384623563
32-33	22.21521117066946	27.05380619932658	28.788243181774963	21.942739448229002
34-35	22.3449834248407	27.132649927392837	28.772969021521444	21.749397626245024
36-37	22.343869727875827	26.9417787029335	28.76711276291473	21.947238806275944
38-39	22.149531891336316	27.149086024470613	28.796319253046715	21.905062831146353
40-41	22.45887559308958	27.01658761573733	28.61823570616224	21.906301085010856
42-43	22.34256398292799	27.10384162005562	28.479105352154605	22.07448904486179
44-45	22.395596828411712	27.237644289293115	28.36259558793627	22.004163294358904
46-47	22.458252908196002	27.191689759822324	28.070663195738348	22.279394136243326
48-49	22.37104448490848	27.344645241324205	28.11728091116599	22.16702936260132
50-51	22.363656912096392	27.51819485023147	27.96422621258896	22.15392202508318
52-53	22.569684171068918	27.463092996495632	27.779205240859877	22.18801759157557
54-55	22.51902282783258	27.310591616725443	27.887553987632458	22.28283156780952
56-57	22.52336913236051	27.46517619762755	27.805638443666332	22.20581622634561
58-59	22.48936848493443	27.47170093633262	27.863005454077495	22.175925124655457
60-61	22.539615506781114	27.4154411839191	27.940691489900534	22.104251819399256
62-63	22.498597197934775	27.450797575350066	27.879651776151015	22.17095345056414
64-65	22.44408301959525	27.43459628416285	27.90133657967892	22.219984116562983
66-67	22.617773343270624	27.43241459490835	27.753601259799677	22.19621080202135
68-69	22.508097222259373	27.399774304721657	27.86077139031478	22.23135708270419
70-71	22.480832658375977	27.218059300646384	27.928166856499647	22.37294118447799
72-73	22.5812095997136	27.28078081402156	27.84091682641999	22.29709275984485
74-75	22.341732422214967	27.4062686465241	27.86746680426245	22.384532126998483
76-77	22.512314363210216	27.320224267801375	27.76868279098829	22.39877857800012
78-79	22.48222406412647	27.427092742147707	27.685571143673027	22.405112050052793
80-81	22.52213774849908	27.386270890560038	27.663462052834163	22.428129308106712
82-83	22.557010893376805	27.361388806755254	27.773533805192617	22.308066494675323
84-85	22.680654037980027	27.307662606690275	27.640182145383925	22.37150120994577
86-87	22.413874606807692	27.350692181081058	27.773492488109213	22.461940724002037
88-89	22.553537933897612	27.38305627130655	27.662604300750665	22.400801494045176
90-91	22.601395326060008	27.38695317821056	27.665226628339905	22.346424867389526
92-93	22.60015549892788	27.399448548717782	27.66408752390481	22.336308428449527
94-95	22.51966597325892	27.494270827253064	27.607378620961132	22.378684578526887
96-97	22.57731161320755	27.57658668889857	27.719960607786128	22.126141090107758
98-99	23.656000135797523	29.536392441026614	27.843828822621102	18.963778600554758
100	22.624918829564137	30.432701154407077	21.790692051152202	25.151687964876583
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	684.0
1	704.5
2	986.0
3	1376.5
4	1801.5
5	2605.5
6	3416.0
7	4249.0
8	5550.5
9	6460.0
10	6325.5
11	6159.0
12	6764.5
13	8285.5
14	11169.5
15	14651.5
16	17882.0
17	20492.5
18	21055.5
19	19635.0
20	17601.5
21	15812.5
22	15453.5
23	17184.5
24	20877.0
25	26643.0
26	34840.5
27	44723.5
28	53938.5
29	63734.5
30	73672.5
31	82937.5
32	94167.5
33	106198.0
34	118555.5
35	130515.0
36	143666.5
37	155170.0
38	162277.5
39	169490.0
40	178905.0
41	186779.0
42	191597.5
43	196926.5
44	202245.0
45	206932.5
46	209539.0
47	210313.5
48	211193.0
49	210161.0
50	206972.0
51	202212.5
52	195256.0
53	185921.5
54	174846.0
55	162748.5
56	150046.0
57	136707.0
58	123006.5
59	108523.0
60	91666.5
61	74249.0
62	58747.0
63	45510.5
64	34448.5
65	25851.5
66	19143.0
67	14557.5
68	11200.5
69	8125.0
70	5864.0
71	4261.5
72	3022.5
73	2082.0
74	1403.5
75	945.5
76	618.5
77	377.0
78	244.5
79	162.0
80	106.0
81	69.5
82	49.0
83	34.0
84	24.0
85	15.0
86	9.0
87	5.0
88	3.5
89	4.5
90	3.5
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005488640943641177
3	2.430394513789451E-4
4	6.68358491292099E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.0127134014997475E-5
24-25	5.063915731580867E-5
26-27	6.887729701987181E-4
28-29	0.0013781075057611987
30-31	6.89850933358168E-4
32-33	6.81327200981762E-4
34-35	0.0010607663036975967
36-37	0.0
38-39	1.5463566805701314E-4
40-41	7.992917237286564E-4
42-43	4.3898541690445044E-4
44-45	0.0
46-47	0.0
48-49	0.001716366563548794
50-51	0.0038499842810332794
52-53	0.0
54-55	0.0037658307620284806
56-57	0.04150525273876561
58-59	0.060182491436856146
60-61	0.03287782949616133
62-63	0.00741754194228676
64-65	0.0
66-67	0.0
68-69	9.3041355603247E-5
70-71	0.0032152831561407216
72-73	0.008949252411888632
74-75	0.006613519753413602
76-77	0.007562859950887174
78-79	0.010731809764755813
80-81	0.01176500753070894
82-83	0.04449957846740431
84-85	0.026708387383047743
86-87	0.0011723305780623418
88-89	0.0018708304820137466
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	27.0
20-21	136.0
22-23	353.0
24-25	413.0
26-27	1460.0
28-29	3842.0
30-31	11218.0
32-33	13779.0
34-35	18650.0
36-37	28942.0
38-39	33765.0
40-41	32671.0
42-43	36161.0
44-45	42277.0
46-47	42750.0
48-49	38762.0
50-51	35318.0
52-53	33954.0
54-55	36234.0
56-57	37017.0
58-59	36829.0
60-61	35825.0
62-63	34601.0
64-65	35896.0
66-67	37923.0
68-69	38262.0
70-71	37677.0
72-73	35474.0
74-75	36193.0
76-77	37531.0
78-79	38263.0
80-81	37944.0
82-83	36027.0
84-85	35746.0
86-87	37241.0
88-89	38550.0
90-91	38775.0
92-93	38664.0
94-95	45551.0
96-97	151907.0
98-99	337027.0
100-101	3287835.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.36436106197382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.5134993429341	79.95018398640799
2	6.106421796755005	10.669672772968225
3	1.316948239522268	3.4516302449266263
4	0.47514736687786513	1.6604378447025583
5	0.21888106475901054	0.9561202185617738
6	0.1173571108005201	0.6151697400700024
7	0.07500128933684794	0.4586707805216566
8	0.04362835079010654	0.30492503927722553
9	0.02785541295554847	0.21902133194810278
>10	0.10239717743159013	1.3656631880561214
>50	0.0017284118828782475	0.10246432110459552
>100	0.0010644469169111771	0.1785664994794971
>500	3.499451866907478E-5	0.025253081292250974
>1k	3.4994518668885914E-5	0.042220950683305544
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.0253287614912092E-5	0.0	0.0	0.0
5	2.0253287614912092E-5	2.0253287614912092E-5	0.0	0.0	0.0
6	8.101315045964837E-5	2.0253287614912092E-5	0.0	0.0	0.0
7	8.101315045964837E-5	2.0253287614912092E-5	0.0	0.0	0.0
8	8.101315045964837E-5	2.0253287614912092E-5	0.0	0.0	0.0
9	8.101315045964837E-5	2.0253287614912092E-5	0.0	0.0	0.0
10-11	8.101315045964837E-5	2.0253287614912092E-5	0.0	0.0	0.0
12-13	1.0126643807456045E-4	2.0253287614912092E-5	0.0	0.0	0.0
14-15	1.1139308188201649E-4	1.0126643807456046E-4	0.0	0.0	0.0
16-17	1.4177301330438463E-4	1.2151972568947254E-4	0.0	3.0379931422368136E-5	0.0
18-19	1.4177301330438463E-4	1.620263009192967E-4	0.0	4.0506575229824183E-5	0.0
20-21	1.4177301330438463E-4	1.8227958853420882E-4	0.0	5.063321903728023E-5	0.0
22-23	1.4177301330438463E-4	1.8227958853420882E-4	0.0	7.088650665219233E-5	0.0
24-25	1.6202630091929673E-4	2.0253287614912092E-4	0.0	8.101315045964837E-5	0.0
26-27	1.6202630091929673E-4	2.22786163764033E-4	0.0	1.8227958853420882E-4	0.0
28-29	1.6202630091929673E-4	2.22786163764033E-4	0.0	4.962055465653463E-4	0.0
30-31	1.6202630091929673E-4	2.22786163764033E-4	0.0	0.0014278567768513024	0.0
32-33	1.6202630091929673E-4	2.430394513789451E-4	0.0	0.0034329322507275994	2.0253287614912092E-5
34-35	1.6202630091929673E-4	3.0379931422368136E-4	0.0	0.005883580052131963	2.0253287614912092E-5
36-37	1.6202630091929673E-4	3.6455917706841763E-4	0.0	0.009863351068462188	2.0253287614912092E-5
38-39	1.6202630091929673E-4	3.8481246468332974E-4	0.0	0.015179839067376612	2.0253287614912092E-5
40-41	1.8227958853420882E-4	4.050657522982418E-4	0.0	0.020577340216750683	2.0253287614912092E-5
42-43	1.8227958853420882E-4	4.151923961056978E-4	0.0	0.026005221297547124	2.0253287614912092E-5
44-45	1.8227958853420882E-4	4.3544568372061E-4	0.0	0.03236475360862952	2.0253287614912092E-5
46-47	1.8227958853420882E-4	4.6582561514297807E-4	0.0	0.03906859180916542	2.0253287614912092E-5
48-49	1.8227958853420882E-4	4.759522589504341E-4	0.0	0.0455698971335522	4.0506575229824183E-5
50-51	1.8227958853420882E-4	6.177252722548187E-4	0.0	0.05187879622559732	4.0506575229824183E-5
52-53	1.8227958853420882E-4	6.278519160622748E-4	0.0	0.057549716757772706	5.063321903728023E-5
54-55	1.8227958853420882E-4	6.278519160622748E-4	0.0	0.06366620961747615	6.075986284473627E-5
56-57	1.8227958853420882E-4	6.278519160622748E-4	0.0	0.06990422220286907	6.075986284473627E-5
58-59	2.025328761491209E-4	6.278519160622748E-4	0.0	0.076142234788262	6.075986284473627E-5
60-61	2.025328761491209E-4	6.379785598697309E-4	0.0	0.08222834771654308	6.075986284473627E-5
62-63	2.025328761491209E-4	6.78485135099555E-4	0.0	0.0875954689344948	6.075986284473627E-5
64-65	2.025328761491209E-4	7.493716417517473E-4	0.0	0.09318537631621053	6.075986284473627E-5
66-67	2.025328761491209E-4	7.493716417517473E-4	0.0	0.09848161102751005	6.075986284473627E-5
68-69	2.025328761491209E-4	7.493716417517473E-4	0.0	0.1037474658073872	7.088650665219233E-5
70-71	2.025328761491209E-4	7.493716417517473E-4	0.0	0.10872977456065555	8.101315045964837E-5
72-73	2.025328761491209E-4	7.493716417517473E-4	0.0	0.11245637948179939	1.0126643807456045E-4
74-75	2.025328761491209E-4	7.797515731741156E-4	0.0	0.11526145981646471	1.0126643807456045E-4
76-77	2.025328761491209E-4	8.101315045964836E-4	0.0	0.11792476713782565	1.0126643807456045E-4
78-79	2.025328761491209E-4	8.101315045964836E-4	0.0	0.1199197159678945	1.0126643807456045E-4
80-81	2.025328761491209E-4	8.101315045964836E-4	0.0	0.12064883432203133	1.1139308188201649E-4
82-83	2.025328761491209E-4	8.101315045964836E-4	0.0	0.12100326685529228	1.2151972568947254E-4
84-85	2.025328761491209E-4	8.202581484039397E-4	0.0	0.1211855464438265	1.2151972568947254E-4
86-87	2.025328761491209E-4	8.506380798263078E-4	0.0	0.12132731945713088	1.2151972568947254E-4
88	2.025328761491209E-4	9.316512302859561E-4	0.0	0.12135769938855324	1.6202630091929673E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6770	0.0	51.73677	1
GTATCAA	12380	0.0	38.527065	1
TCAACGC	14380	0.0	32.57397	4
TATCAAC	14605	0.0	32.248707	2
ATCAACG	14610	0.0	32.06117	3
CAACGCA	14775	0.0	31.731997	5
AACGCAG	15385	0.0	30.584772	6
ACGCAGA	17545	0.0	26.697842	7
CGCAGAG	18045	0.0	25.934444	8
GATCAAC	2120	0.0	25.355927	1
GTGGTAT	3255	0.0	22.805681	1
TGGTATC	2885	0.0	22.77297	2
GCAGAGT	20990	0.0	22.295713	9
GAGTACT	12400	0.0	20.986275	12-13
TACATGG	15545	0.0	20.66565	2
GTACATG	15660	0.0	20.568375	1
ACATGGG	15340	0.0	20.1901	3
CAGAGTA	20285	0.0	19.94755	10-11
GTACTTT	13755	0.0	18.857084	14-15
AGAGTAC	19045	0.0	18.323126	10-11
>>END_MODULE