FastQCFastQC Report
Fri 27 May 2016
SRR2140115_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140115_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5169372
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC91950.057.004311
GGTATCA108650.053.1991651
TATACAC126750.046.444673
TTATACA123650.046.3133542
GTATCAA210300.039.0184971
TATCAAC245750.033.122332
GATCAAC28050.031.8035871
ATCAACG255100.031.79493
TGGTATC41250.031.6922042
GTGGTAT44000.031.5800861
TCAACGC258500.031.4400184
TACACAT189350.031.3714775
CAACGCA264200.030.7302935
AACGCAG275050.029.6376326
ATACACA212100.028.8402424
ACACATC209700.027.7781876
GCAACGC6200.027.1857571
ACATCTC220600.026.4429158
ACGCAGA312150.026.049267
CGCAGAG315550.025.7555548
Produced by FastQC (version 0.11.5)