##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140115_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5169372
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.128208029911562	25.0	18.0	33.0	18.0	33.0
2	29.0083749051142	30.0	27.0	32.0	25.0	33.0
3	27.852313588575168	29.0	25.0	31.0	18.0	33.0
4	31.48179933655384	33.0	31.0	33.0	29.0	33.0
5	32.11922995675297	33.0	33.0	33.0	31.0	33.0
6	36.45693384031948	38.0	37.0	38.0	34.0	38.0
7	36.66368932241673	38.0	37.0	38.0	34.0	38.0
8	36.92743934853208	38.0	38.0	38.0	35.0	38.0
9	37.31993673506182	38.0	38.0	38.0	36.0	38.0
10-11	37.31177626605321	38.0	38.0	38.0	36.5	38.0
12-13	37.42317645160766	38.0	38.0	38.0	37.0	38.0
14-15	37.37396824991508	38.0	38.0	38.0	37.0	38.0
16-17	37.46384647883727	38.0	38.0	38.0	37.0	38.0
18-19	37.47881125599009	38.0	38.0	38.0	37.0	38.0
20-21	37.49710902307362	38.0	38.0	38.0	37.0	38.0
22-23	37.51278635161569	38.0	38.0	38.0	37.5	38.0
24-25	37.515703006091634	38.0	38.0	38.0	37.5	38.0
26-27	37.473920028844454	38.0	38.0	38.0	37.0	38.0
28-29	37.45909439078294	38.0	38.0	38.0	37.0	38.0
30-31	37.45220424214837	38.0	38.0	38.0	37.0	38.0
32-33	37.44036989141421	38.0	38.0	38.0	37.0	38.0
34-35	37.397462093168286	38.0	38.0	38.0	37.0	38.0
36-37	37.36839237723205	38.0	38.0	38.0	37.0	38.0
38-39	37.31556995909975	38.0	38.0	38.0	37.0	38.0
40-41	37.37578237399421	38.0	38.0	38.0	37.0	38.0
42-43	37.33495891368186	38.0	38.0	38.0	37.0	38.0
44-45	37.269906098869804	38.0	38.0	38.0	37.0	38.0
46-47	37.20926858896073	38.0	38.0	38.0	37.0	38.0
48-49	37.14271431706898	38.0	38.0	38.0	37.0	38.0
50-51	37.09941613768564	38.0	38.0	38.0	36.5	38.0
52-53	37.06472961706014	38.0	38.0	38.0	36.0	38.0
54-55	37.04065173515696	38.0	38.0	38.0	36.0	38.0
56-57	36.99533637223657	38.0	38.0	38.0	36.0	38.0
58-59	36.95366526378838	38.0	38.0	38.0	36.0	38.0
60-61	36.962213372580926	38.0	38.0	38.0	36.0	38.0
62-63	36.98015947867876	38.0	38.0	38.0	36.0	38.0
64-65	36.997422753633934	38.0	38.0	38.0	36.0	38.0
66-67	36.990370861778885	38.0	38.0	38.0	36.0	38.0
68-69	36.950522176148866	38.0	38.0	38.0	36.0	38.0
70-71	36.959254216599845	38.0	38.0	38.0	36.0	38.0
72-73	36.98222173080306	38.0	38.0	38.0	36.0	38.0
74-75	37.011799903611944	38.0	38.0	38.0	36.0	38.0
76-77	37.00494768181104	38.0	38.0	38.0	36.0	38.0
78-79	36.9792205343267	38.0	38.0	38.0	36.0	38.0
80-81	36.940723484794056	38.0	38.0	38.0	36.0	38.0
82-83	36.90870273438377	38.0	38.0	38.0	35.5	38.0
84-85	36.890512890961205	38.0	38.0	38.0	35.0	38.0
86-87	36.9204774000998	38.0	38.0	38.0	35.0	38.0
88-89	36.88666035684447	38.0	38.0	38.0	35.0	38.0
90-91	36.90399124084716	38.0	38.0	38.0	35.0	38.0
92-93	36.89902758279243	38.0	38.0	38.0	35.0	38.0
94-95	36.92023037055142	38.0	38.0	38.0	35.0	38.0
96-97	36.923721317095655	38.0	38.0	38.0	35.0	38.0
98-99	36.91331770950831	38.0	38.0	38.0	35.0	38.0
100	35.413264631705964	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	5.0
19	16.0
20	48.0
21	155.0
22	380.0
23	1002.0
24	2211.0
25	4219.0
26	7648.0
27	13460.0
28	22524.0
29	33599.0
30	44531.0
31	56164.0
32	70180.0
33	94917.0
34	149517.0
35	309063.0
36	1096790.0
37	3262941.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.12454975188476	19.080847731600667	10.952084701971536	21.842517814543044
2	14.430149068070646	20.07115337925351	42.625343022935255	22.873354529740585
3	18.808355847394708	27.21210083565438	30.225896348524245	23.753646968426665
4	12.210439284403673	18.89903138486371	37.8030150469915	31.087514283741115
5	12.895957187836357	38.55909383190067	34.70549227256232	13.839456707700664
6	28.575095775657083	37.29774138908943	19.05051522699469	15.076647608258797
7	26.125997509948984	33.55003276993801	21.41983591043554	18.90413380967746
8	24.489106220252673	35.77974268441118	21.397589494429884	18.33356160090626
9	26.03687643295936	16.86444697731175	20.17715111235949	36.9215254773694
10-11	24.23795385590358	26.748935847526546	27.93019152036263	21.082918776207247
12-13	25.712533359951657	24.234297705794823	27.994425628490273	22.058743305763254
14-15	22.927997830297375	25.22942825550183	25.82843718734113	26.014136726859665
16-17	21.858854421775025	29.1532994723537	26.339350311798025	22.648495794073245
18-19	21.982912044248316	27.14487369065333	28.863254569413847	22.008959695684503
20-21	23.225714282121647	25.999185382697476	28.6691995055035	22.10590082967737
22-23	22.687542107409016	26.822162002951206	28.64368801172744	21.846607877912334
24-25	22.769213058916225	26.885498686553216	28.975888783391802	21.369399471138752
26-27	22.548600057684496	26.944986775234526	28.984484883363056	21.521928283717926
28-29	21.85813832434496	26.352194030817373	29.43334072616733	22.356326918670337
30-31	22.00257073969995	26.518303746169074	29.79965702139946	21.67946849273151
32-33	21.01321238913097	26.105539634736317	30.55555825674647	22.32568971938624
34-35	21.221336059174597	26.317666188434057	29.93381140976192	22.527186342629424
36-37	21.54251944246881	26.574306822733107	30.237282373551942	21.64589136124614
38-39	21.5112821624411	26.22850119811669	30.123782801789524	22.136433837652692
40-41	21.857446777932054	26.094941797657533	30.218828671370545	21.828782753039864
42-43	22.392536566086733	25.886576727324417	29.966513846158183	21.754372860430667
44-45	21.68821198892719	26.667468012520928	29.718327949163758	21.925992049388125
46-47	22.539138474502295	25.90268594962649	28.81606236839394	22.742113207477267
48-49	22.220930931240527	25.998550630009415	28.814877088363772	22.96564135038629
50-51	21.714122931295744	26.353833673261633	29.29243961140584	22.639603784036783
52-53	21.87811726976885	26.57267704710524	29.82184934472033	21.72735633840557
54-55	21.976230409578033	26.723186967507583	28.8501424699453	22.450440152969087
56-57	22.600303551040057	27.578057336190632	27.726332928114616	22.09530618465469
58-59	21.43847085697895	28.594072370225327	28.855512997858	21.111943774937718
60-61	22.172460732347187	29.709825638733722	27.092719616485972	21.024994012433122
62-63	21.2989385309883	30.406386740774156	27.144263751275822	21.150410976961723
64-65	21.33334768722049	30.183448343854163	27.25485874358537	21.228345225339975
66-67	21.70089155642939	29.76715753902453	27.24044926785045	21.291501636695624
68-69	21.773484719546598	29.069737803880365	27.61996145221481	21.536816024358235
70-71	21.879727313333944	28.19227538109972	28.018739651786667	21.90925765377967
72-73	22.26607214219244	27.40607735856715	28.298673380511236	22.029177118729176
74-75	22.146213222330793	27.408019601766515	28.237386209384784	22.20838096651791
76-77	22.205378604826308	27.339140803966483	28.233282078639345	22.222198512567857
78-79	22.2713942982406	27.39340158578358	28.080193453911818	22.25501066206401
80-81	22.251301562286223	27.371178509228976	28.1034198092585	22.274100119226294
82-83	22.358657341600964	27.296926856488753	28.208223916175985	22.136191885734302
84-85	22.51649523424181	27.28573054236709	27.998565952487827	22.19920827090327
86-87	22.218434512474463	27.365252669769212	28.13007252820301	22.286240289553312
88-89	22.41205872945583	27.31405943819637	27.999525888177573	22.27435594417023
90-91	22.46227322350044	27.30937636479692	28.023911993114936	22.204438418587706
92-93	22.460076958247072	27.347347582759856	27.92907888739098	22.26349657160209
94-95	22.276037131259578	27.515189920602378	27.944773403296143	22.2639995448419
96-97	22.43565593592085	27.56914062093531	27.963142487387476	22.03206095575636
98-99	23.466012637431177	29.51812681438143	28.14330234496681	18.87255820322059
100	30.09844993807343	40.565501469965284	29.336048591961283	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1184.0
1	1215.5
2	1697.0
3	2358.0
4	3082.0
5	4486.5
6	5814.0
7	7109.5
8	9275.5
9	10634.5
10	10229.0
11	9865.5
12	10784.5
13	13472.0
14	18723.5
15	24852.0
16	30380.0
17	34765.5
18	35143.5
19	31257.5
20	25929.0
21	20840.5
22	18068.5
23	18593.0
24	21414.5
25	26392.5
26	34541.0
27	44364.0
28	53196.5
29	63217.5
30	73822.5
31	84276.5
32	97149.5
33	112062.0
34	126022.0
35	139550.5
36	154568.0
37	166382.5
38	174382.5
39	180826.5
40	188613.0
41	195650.0
42	200624.0
43	207910.0
44	232659.5
45	273130.5
46	281173.5
47	268226.5
48	253361.0
49	230638.5
50	217749.0
51	209507.0
52	199768.5
53	189674.0
54	178360.5
55	164774.5
56	150857.0
57	135820.5
58	121050.5
59	105818.0
60	89307.5
61	72198.0
62	55687.0
63	42860.5
64	33309.0
65	25082.5
66	17802.0
67	12921.5
68	9710.0
69	7084.0
70	5107.5
71	3714.0
72	2663.0
73	1848.5
74	1244.5
75	808.5
76	546.5
77	364.0
78	248.5
79	171.5
80	108.5
81	68.5
82	52.5
83	40.0
84	26.0
85	17.5
86	12.0
87	9.0
88	7.5
89	7.5
90	7.5
91	5.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004971590359525296
3	1.9344709570137341E-4
4	6.383754158145322E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.9345826674741616E-5
24-25	2.9021095240656465E-5
26-27	7.353219341018028E-4
28-29	0.0015488892866522628
30-31	8.338303188266572E-4
32-33	7.973851988970412E-4
34-35	0.0011615665921734814
36-37	0.0
38-39	1.9786372472328017E-4
40-41	0.0010778834680221917
42-43	4.127364841636535E-4
44-45	0.0
46-47	0.0
48-49	0.0016140876739101366
50-51	0.0037265448739164596
52-53	0.0
54-55	0.0036039885856532935
56-57	0.04063898962286866
58-59	0.0590301332691424
60-61	0.03212617791377798
62-63	0.00712765074198061
64-65	0.0
66-67	0.0
68-69	1.1745926219139048E-5
70-71	0.0034302955547447582
72-73	0.007994368629410865
74-75	0.005684010469004085
76-77	0.006903216899074968
78-79	0.010299039141555404
80-81	0.011275848790482
82-83	0.04577959513499709
84-85	0.02696672769293324
86-87	9.324872565625456E-4
88-89	0.001442810951717208
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	44.0
20-21	173.0
22-23	414.0
24-25	642.0
26-27	1986.0
28-29	5707.0
30-31	14493.0
32-33	18095.0
34-35	24380.0
36-37	37957.0
38-39	45146.0
40-41	42396.0
42-43	47249.0
44-45	57210.0
46-47	58130.0
48-49	53549.0
50-51	47193.0
52-53	42195.0
54-55	44464.0
56-57	46523.0
58-59	47072.0
60-61	52505.0
62-63	54641.0
64-65	69356.0
66-67	80778.0
68-69	79399.0
70-71	105127.0
72-73	52655.0
74-75	44955.0
76-77	45944.0
78-79	47864.0
80-81	48471.0
82-83	44680.0
84-85	43455.0
86-87	44983.0
88-89	46369.0
90-91	47389.0
92-93	45849.0
94-95	51042.0
96-97	145405.0
98-99	1091256.0
100-101	2292231.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.33864304098067
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.40204218779809	78.05189650627975
2	6.844105876787827	11.818216284613243
3	1.6149151896847518	4.182887583109481
4	0.5683938908614629	1.9629742899904785
5	0.23571725532734855	1.0175753983153821
6	0.12145018147423123	0.6291506319339584
7	0.06756084615157916	0.4083178245599464
8	0.03998695805702528	0.27619357583841375
9	0.02690275637919049	0.20904727318572536
>10	0.07573896146849735	0.990388989901639
>50	0.0018114160055220858	0.10696338236780109
>100	0.0012383304202573404	0.24047597788711558
>500	1.021121888568916E-4	0.06424091296296426
>1k	3.403739536534678E-5	0.04167136905403648
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.868941914027468E-5	0.0
2	0.0	0.0	0.0	3.868941914027468E-5	0.0
3	0.0	0.0	0.0	5.803412871041202E-5	0.0
4	0.0	0.0	0.0	5.803412871041202E-5	0.0
5	0.0	0.0	0.0	5.803412871041202E-5	0.0
6	0.0	0.0	0.0	7.737883828054936E-5	3.868941914027468E-5
7	0.0	0.0	0.0	7.737883828054936E-5	3.868941914027468E-5
8	0.0	0.0	0.0	1.1606825742082404E-4	3.868941914027468E-5
9	0.0	0.0	0.0	1.7410238613123607E-4	3.868941914027468E-5
10-11	0.0	0.0	0.0	3.191877079072661E-4	3.868941914027468E-5
12-13	0.0	0.0	0.0	4.6427302968329615E-4	3.868941914027468E-5
14-15	0.0	0.0	0.0	4.6427302968329615E-4	7.737883828054935E-5
16-17	0.0	0.0	0.0	4.8361773925343347E-4	1.1606825742082404E-4
18-19	0.0	0.0	0.0	5.609965775339829E-4	1.4508532177603007E-4
20-21	0.0	0.0	0.0	6.577201253846695E-4	1.7410238613123607E-4
22-23	1.934470957013734E-5	0.0	0.0	7.060818993100129E-4	2.1279180527151073E-4
24-25	1.934470957013734E-5	0.0	0.0	7.350989636652189E-4	2.514812244117854E-4
26-27	1.934470957013734E-5	0.0	0.0	9.575631237217982E-4	2.611535791968541E-4
28-29	1.934470957013734E-5	0.0	0.0	0.002021522150079352	2.7082593398192276E-4
30-31	1.934470957013734E-5	0.0	0.0	0.0033853241747740345	2.7082593398192276E-4
32-33	1.934470957013734E-5	0.0	0.0	0.006045221740667919	2.7082593398192276E-4
34-35	1.934470957013734E-5	0.0	0.0	0.009256443529310716	2.901706435520601E-4
36-37	1.934470957013734E-5	0.0	0.0	0.014547221596743279	2.901706435520601E-4
38-39	1.934470957013734E-5	0.0	0.0	0.02211100303866698	2.9984299833712877E-4
40-41	1.934470957013734E-5	0.0	0.0	0.029781180383226434	3.0951535312219745E-4
42-43	1.934470957013734E-5	0.0	0.0	0.0368613440858967	3.0951535312219745E-4
44-45	1.934470957013734E-5	0.0	0.0	0.044086593110343	3.0951535312219745E-4
46-47	1.934470957013734E-5	0.0	0.0	0.051476272166135456	3.191877079072661E-4
48-49	1.934470957013734E-5	0.0	0.0	0.05863381470708628	3.3853241747740345E-4
50-51	1.934470957013734E-5	0.0	0.0	0.06588808079588777	3.4820477226247214E-4
52-53	1.934470957013734E-5	0.0	0.0	0.07344218988302642	3.4820477226247214E-4
54-55	1.934470957013734E-5	0.0	0.0	0.08070612832661297	3.4820477226247214E-4
56-57	1.934470957013734E-5	0.0	0.0	0.0877379302553579	3.578771270475408E-4
58-59	1.934470957013734E-5	0.0	0.0	0.09473104276496255	3.6754948183260946E-4
60-61	1.934470957013734E-5	0.0	0.0	0.10146300169537034	3.772218366176781E-4
62-63	1.934470957013734E-5	0.0	0.0	0.10764363640302924	4.0623890097288414E-4
64-65	1.934470957013734E-5	0.0	0.0	0.11395968407767906	4.0623890097288414E-4
66-67	1.934470957013734E-5	0.0	0.0	0.1201209740757678	4.1591125575795283E-4
68-69	1.934470957013734E-5	0.0	0.0	0.1257696292702479	4.4492832011315883E-4
70-71	1.934470957013734E-5	0.0	0.0	0.131273199142952	4.4492832011315883E-4
72-73	1.934470957013734E-5	0.0	0.0	0.1358675676658596	4.8361773925343347E-4
74-75	1.934470957013734E-5	0.0	0.0	0.13934961538848434	4.8361773925343347E-4
76-77	1.934470957013734E-5	0.0	0.0	0.14268657778933302	4.8361773925343347E-4
78-79	1.934470957013734E-5	0.0	0.0	0.14493056409946894	4.8361773925343347E-4
80-81	1.934470957013734E-5	0.0	0.0	0.14560762893442375	5.029624488235708E-4
82-83	1.934470957013734E-5	0.0	0.0	0.1460235401901817	5.706689323190515E-4
84-85	1.934470957013734E-5	0.0	0.0	0.14626534905980842	5.803412871041202E-4
86-87	1.934470957013734E-5	0.0	0.0	0.1464587961555098	5.803412871041202E-4
88	1.934470957013734E-5	0.0	0.0	0.14647814086507993	5.996859966742575E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	9195	0.0	57.00431	1
GGTATCA	10865	0.0	53.199165	1
TATACAC	12675	0.0	46.44467	3
TTATACA	12365	0.0	46.313354	2
GTATCAA	21030	0.0	39.018497	1
TATCAAC	24575	0.0	33.12233	2
GATCAAC	2805	0.0	31.803587	1
ATCAACG	25510	0.0	31.7949	3
TGGTATC	4125	0.0	31.692204	2
GTGGTAT	4400	0.0	31.580086	1
TCAACGC	25850	0.0	31.440018	4
TACACAT	18935	0.0	31.371477	5
CAACGCA	26420	0.0	30.730293	5
AACGCAG	27505	0.0	29.637632	6
ATACACA	21210	0.0	28.840242	4
ACACATC	20970	0.0	27.778187	6
GCAACGC	620	0.0	27.185757	1
ACATCTC	22060	0.0	26.442915	8
ACGCAGA	31215	0.0	26.04926	7
CGCAGAG	31555	0.0	25.755554	8
>>END_MODULE