FastQCFastQC Report
Fri 27 May 2016
SRR2140114_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140114_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4999335
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67800.047.868211
GTATCAA124400.037.8666271
CTTATAC43850.037.4920851
TCAACGC143450.032.2425544
ATCAACG143950.032.012223
TATCAAC146150.031.7067662
CAACGCA147950.031.290665
AACGCAG154750.029.9432146
TATACAC67300.028.7303523
ACGCAGA174650.026.4826477
TTATACA69200.025.9733642
CGCAGAG178550.025.9041988
GTGGTAT34350.024.1785221
TGGTATC32600.023.3860742
GATCAAC20350.022.3944931
GCAGAGT207250.022.2553339
TACACAT90650.021.6587475
ACACATC87200.020.9527476
ACATCTC88700.020.6464318
GTACATG151100.020.1541041
Produced by FastQC (version 0.11.5)