##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140114_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4999335
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.14088813812237	25.0	18.0	33.0	18.0	33.0
2	29.051138201380784	30.0	27.0	32.0	25.0	33.0
3	28.01112948022087	29.0	27.0	31.0	18.0	33.0
4	31.579083218068003	33.0	31.0	33.0	29.0	33.0
5	32.14846154538554	33.0	33.0	33.0	31.0	33.0
6	36.4850809157618	38.0	37.0	38.0	34.0	38.0
7	36.73400642285424	38.0	37.0	38.0	34.0	38.0
8	36.95398087945697	38.0	38.0	38.0	35.0	38.0
9	37.31593521938418	38.0	38.0	38.0	36.0	38.0
10-11	37.32019148546756	38.0	38.0	38.0	36.5	38.0
12-13	37.4221323436017	38.0	38.0	38.0	37.0	38.0
14-15	37.36534489087049	38.0	38.0	38.0	37.0	38.0
16-17	37.45032609337042	38.0	38.0	38.0	37.0	38.0
18-19	37.466223607739835	38.0	38.0	38.0	37.0	38.0
20-21	37.48639849250978	38.0	38.0	38.0	37.0	38.0
22-23	37.50226266037159	38.0	38.0	38.0	37.0	38.0
24-25	37.508553016184806	38.0	38.0	38.0	37.0	38.0
26-27	37.47586409886104	38.0	38.0	38.0	37.0	38.0
28-29	37.45407270561645	38.0	38.0	38.0	37.0	38.0
30-31	37.45407429322447	38.0	38.0	38.0	37.0	38.0
32-33	37.44004661203077	38.0	38.0	38.0	37.0	38.0
34-35	37.405454559454	38.0	38.0	38.0	37.0	38.0
36-37	37.38218750278547	38.0	38.0	38.0	37.0	38.0
38-39	37.354026708781795	38.0	38.0	38.0	37.0	38.0
40-41	37.3707138857932	38.0	38.0	38.0	37.0	38.0
42-43	37.333440243777886	38.0	38.0	38.0	37.0	38.0
44-45	37.287158632979285	38.0	38.0	38.0	37.0	38.0
46-47	37.249973357850905	38.0	38.0	38.0	37.0	38.0
48-49	37.20116353921824	38.0	38.0	38.0	37.0	38.0
50-51	37.16311903992748	38.0	38.0	38.0	37.0	38.0
52-53	37.12567192204594	38.0	38.0	38.0	36.0	38.0
54-55	37.103146306351476	38.0	38.0	38.0	36.0	38.0
56-57	37.056901501033565	38.0	38.0	38.0	36.0	38.0
58-59	37.022737696900116	38.0	38.0	38.0	36.0	38.0
60-61	37.04219926321066	38.0	38.0	38.0	36.0	38.0
62-63	37.05298650401027	38.0	38.0	38.0	36.0	38.0
64-65	37.05838415199452	38.0	38.0	38.0	36.0	38.0
66-67	37.04861301060795	38.0	38.0	38.0	36.0	38.0
68-69	37.00774162759717	38.0	38.0	38.0	36.0	38.0
70-71	37.0026762616947	38.0	38.0	38.0	36.0	38.0
72-73	37.0154616175694	38.0	38.0	38.0	36.0	38.0
74-75	37.030853904548906	38.0	38.0	38.0	36.0	38.0
76-77	37.011318835851654	38.0	38.0	38.0	36.0	38.0
78-79	36.97314369069309	38.0	38.0	38.0	36.0	38.0
80-81	36.92482526686338	38.0	38.0	38.0	36.0	38.0
82-83	36.88516399564576	38.0	38.0	38.0	35.0	38.0
84-85	36.85603948442767	38.0	38.0	38.0	35.0	38.0
86-87	36.88074002426832	38.0	38.0	38.0	35.0	38.0
88-89	36.83859486824096	38.0	38.0	38.0	35.0	38.0
90-91	36.849105522749184	38.0	38.0	38.0	35.0	38.0
92-93	36.83554951918604	38.0	38.0	38.0	35.0	38.0
94-95	36.8500851505226	38.0	38.0	38.0	35.0	38.0
96-97	36.85071999968907	38.0	38.0	38.0	35.0	38.0
98-99	36.83279766265697	38.0	38.0	38.0	35.0	38.0
100	35.22855572320033	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	39.0
21	86.0
22	279.0
23	950.0
24	2124.0
25	4281.0
26	7691.0
27	12880.0
28	20684.0
29	30033.0
30	40252.0
31	51448.0
32	67166.0
33	91359.0
34	141418.0
35	279993.0
36	983872.0
37	3264775.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.48205511333008	18.677304081442834	11.892161657500449	21.948479147726648
2	14.544858067890967	20.21264061435734	41.89540202178616	23.347099295965528
3	19.09997939723923	25.6029908007673	30.117695771233343	25.17933403076012
4	12.792938218412358	18.146822408314755	38.74984622862578	30.310393144647108
5	13.670218139012489	37.6069817285699	35.05982295645321	13.662977175964402
6	29.989008538135568	37.59035951781587	18.675603855312755	13.745028088735802
7	26.715893213797436	32.816264563186905	22.396638752954143	18.071203470061516
8	24.21590071479507	36.881805280102256	21.02253599728764	17.879758007815038
9	25.374374791847316	17.612382446865432	21.101386484402425	35.911856276884826
10-11	24.228422380176564	26.883965567420464	28.530014491927425	20.357597560475543
12-13	25.24227722287064	24.214420517928883	28.733651575659565	21.80965068354091
14-15	22.348462345491953	25.66968406798104	26.40722216054735	25.574631425979653
16-17	21.86532808863579	29.01300873016111	27.042646672007375	22.07901650919572
18-19	21.842525055832425	27.82431063331423	28.997316643113535	21.33584766773981
20-21	22.533417065399124	26.96253530133692	28.95935304277346	21.54469459049049
22-23	22.25565821395194	27.05087751725109	28.857291226587805	21.836173042209165
24-25	22.128542304199904	27.16718289479449	29.000357888828006	21.703916912177604
26-27	21.98297150115828	27.26833655769269	29.091550964701845	21.657140976447188
28-29	21.75708396846339	27.146115806092126	29.271566534943894	21.82523369050059
30-31	22.187276119223576	26.92465345058876	28.88880407797582	21.99926635221184
32-33	22.10989654223076	27.12345166890503	29.104634979122064	21.662016809742145
34-35	22.163802155792027	27.374845470376354	28.957440139110624	21.503912234721
36-37	21.986377054232683	27.21765813054863	28.88223700685107	21.913727808367614
38-39	21.86998151898652	27.192608894942673	29.129051763091933	21.80835782297887
40-41	22.148210795511293	27.04961463295799	29.05689884852473	21.74527572300598
42-43	22.266308852324627	27.0490749146686	28.90134439644139	21.783271836565376
44-45	22.072526913847078	27.316554364084293	28.767689781964766	21.843228940103863
46-47	22.3574921343163	27.137560464632767	28.29683109477014	22.208116306280793
48-49	22.203898367310767	27.207316507083146	28.39911845753278	22.189666668073308
50-51	22.071311544108482	27.433865889183057	28.40368536079088	22.091137205917576
52-53	22.22966250307407	27.487522884757265	28.44181123510984	21.841003377058822
54-55	22.219687288240635	27.412032272288904	28.234549367437396	22.133731072033058
56-57	22.39381272423436	27.760715637869538	27.914519894975378	21.93095174292073
58-59	22.062446406230233	28.054625230189632	28.225927611510183	21.657000752069948
60-61	22.300443331292684	28.279723529250205	27.788373539394378	21.631459600062726
62-63	22.003372202839287	28.50502172272763	27.789040173223324	21.70256590120976
64-65	22.030666065592246	28.44289417333484	27.7628197903753	21.763619970697622
66-67	22.21269699121639	28.337798854755462	27.673311502232682	21.776192651795462
68-69	22.12957082843816	28.126990299806465	27.909473461344543	21.833965410410837
70-71	22.14992077145249	27.792737793212385	28.030907354314564	22.02643408102056
72-73	22.34903482452203	27.630882687336793	28.026263313650684	21.993819174490493
74-75	22.154276459659382	27.722892040084997	28.019334610072256	22.103496890183372
76-77	22.27344305720124	27.610365565481548	27.951119008269238	22.165072369047977
78-79	22.262204441903474	27.67047520705353	27.91825639440027	22.149063956642728
80-81	22.29875337989304	27.6515805048494	27.869561271801302	22.18010484345626
82-83	22.293766250817	27.59894872966123	28.012242070147312	22.095042949374452
84-85	22.486638269399805	27.549617426290425	27.880385649836516	22.08335865447326
86-87	22.20579756098209	27.630419057454443	27.969841437138598	22.193941944424868
88-89	22.34340723337543	27.58636296571512	27.899207130734826	22.17102267017462
90-91	22.39065359679871	27.603297453236962	27.92174623362389	22.08430271634044
92-93	22.40448205686181	27.59841253516472	27.863512733133906	22.133592674839566
94-95	22.246929166494848	27.707815888503657	27.869975035765155	22.175279909236345
96-97	22.35323756020512	27.791673705648705	27.971755262047537	21.88333347209864
98-99	23.4001097579331	29.723905530380318	28.153098527992555	18.722886183694023
100	22.388506334148914	30.58478085526663	22.093651364613475	24.93306144597098
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	933.0
1	922.0
2	1099.0
3	1394.5
4	1786.5
5	2517.5
6	3245.5
7	4119.0
8	5427.0
9	6273.5
10	6165.5
11	6071.5
12	6684.0
13	8235.0
14	11241.5
15	14866.5
16	18154.5
17	20883.0
18	21680.0
19	20371.0
20	18383.0
21	16692.5
22	16415.5
23	18220.0
24	22110.0
25	28115.5
26	36805.5
27	46864.5
28	56581.0
29	67014.5
30	77607.0
31	88461.5
32	100290.5
33	112655.5
34	125162.0
35	136319.5
36	148474.5
37	160355.5
38	169169.0
39	176798.5
40	185346.0
41	192788.0
42	197978.0
43	204188.5
44	213352.0
45	226399.0
46	229642.0
47	225910.0
48	222439.5
49	213420.5
50	205418.0
51	197896.0
52	188312.5
53	177762.0
54	167090.5
55	154959.5
56	141814.5
57	128021.5
58	113291.0
59	98540.0
60	82766.5
61	67019.5
62	52620.5
63	40636.0
64	30903.0
65	23105.5
66	17008.0
67	12876.5
68	9863.0
69	7184.0
70	5217.0
71	3722.0
72	2606.5
73	1739.5
74	1172.5
75	783.5
76	505.0
77	329.0
78	212.5
79	130.5
80	89.5
81	56.0
82	40.0
83	26.5
84	14.5
85	10.0
86	6.0
87	3.0
88	1.5
89	1.0
90	1.0
91	1.5
92	1.5
93	1.5
94	2.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0058007715026098475
3	1.000133017691353E-4
4	6.000798106148118E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	8.00197968977525E-5
26-27	6.102219987631501E-4
28-29	0.0013809885115765865
30-31	7.713058980159307E-4
32-33	6.624208168752707E-4
34-35	0.0010867899374924718
36-37	0.0
38-39	3.0476512462963417E-4
40-41	8.280469521022884E-4
42-43	3.49801940083866E-4
44-45	0.0
46-47	0.0
48-49	0.0015086006589525478
50-51	0.003531055419702098
52-53	0.0
54-55	0.0035737332977903422
56-57	0.039021104703256745
58-59	0.057349764745228196
60-61	0.030345781377299916
62-63	0.006545152448998352
64-65	0.0
66-67	0.0
68-69	4.607673319647775E-5
70-71	0.0039178378106096215
72-73	0.007933193070072524
74-75	0.005481652551411346
76-77	0.007170456180572299
78-79	0.010305429600304885
80-81	0.011922375634642421
82-83	0.042917917250534336
84-85	0.025204103759232162
86-87	0.001012208883980238
88-89	0.001495860876244617
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	34.0
20-21	123.0
22-23	342.0
24-25	463.0
26-27	1278.0
28-29	3334.0
30-31	9321.0
32-33	12003.0
34-35	16630.0
36-37	26121.0
38-39	31195.0
40-41	30465.0
42-43	34893.0
44-45	41576.0
46-47	42032.0
48-49	39327.0
50-51	35747.0
52-53	34421.0
54-55	36772.0
56-57	38287.0
58-59	38459.0
60-61	40116.0
62-63	39662.0
64-65	44504.0
66-67	49107.0
68-69	49842.0
70-71	57392.0
72-73	40385.0
74-75	39464.0
76-77	41011.0
78-79	42344.0
80-81	41734.0
82-83	39742.0
84-85	39346.0
86-87	40754.0
88-89	41812.0
90-91	42822.0
92-93	42178.0
94-95	49070.0
96-97	152428.0
98-99	334587.0
100-101	3258212.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.47037149600413
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.1881757121265	82.48110328502862
2	5.908779026052026	10.573213090973447
3	1.186350535376804	3.1842966937393817
4	0.36973166821265985	1.3232011883529609
5	0.14978758169514597	0.670077528987639
6	0.07186900793804392	0.3858088103559636
7	0.04041754130966668	0.253132070515168
8	0.023181415416446634	0.1659239879290142
9	0.015523193298735957	0.1249979284117969
>10	0.04438822642692547	0.6044169507247873
>50	9.967495905435244E-4	0.06280433521571067
>100	7.389145021886596E-4	0.12474431468995323
>500	3.0214027497215838E-5	0.017246800487933537
>1k	3.0214026816826807E-5	0.029033014587649127
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	6.0007981061481175E-5	0.0	0.0	0.0
5	0.0	1.2001596212296235E-4	0.0	0.0	0.0
6	1.2001596212296235E-4	1.2001596212296235E-4	0.0	0.0	0.0
7	1.2001596212296235E-4	1.2001596212296235E-4	0.0	0.0	0.0
8	1.2001596212296235E-4	1.2001596212296235E-4	0.0	0.0	0.0
9	1.2001596212296235E-4	2.000266035382706E-4	0.0	0.0	2.000266035382706E-5
10-11	1.2001596212296235E-4	2.000266035382706E-4	0.0	9.001197159222176E-5	2.000266035382706E-5
12-13	1.2001596212296235E-4	2.2002926389209766E-4	0.0	1.3001729229987588E-4	2.000266035382706E-5
14-15	1.2001596212296235E-4	2.700359147766653E-4	0.0	1.400186224767894E-4	2.000266035382706E-5
16-17	1.6002128283061648E-4	2.800372449535788E-4	0.0	2.4003192424592473E-4	2.000266035382706E-5
18-19	1.8002394318444352E-4	2.9003857513049233E-4	0.0	2.800372449535788E-4	2.000266035382706E-5
20-21	1.8002394318444352E-4	3.000399053074059E-4	0.0	2.9003857513049233E-4	2.000266035382706E-5
22-23	1.8002394318444352E-4	3.600478863688871E-4	0.0	3.600478863688871E-4	4.000532070765412E-5
24-25	1.8002394318444352E-4	4.000532070765412E-4	0.0	3.9005187689962765E-4	4.000532070765412E-5
26-27	1.8002394318444352E-4	4.200558674303682E-4	0.0	4.200558674303682E-4	4.000532070765412E-5
28-29	1.8002394318444352E-4	4.3005719760728174E-4	0.0	7.000931123839471E-4	4.000532070765412E-5
30-31	1.8002394318444352E-4	4.800638484918494E-4	0.0	0.001380183564414067	4.000532070765412E-5
32-33	1.8002394318444352E-4	5.000665088456765E-4	0.0	0.003270434967850724	6.0007981061481175E-5
34-35	1.9002527336135707E-4	5.900784804378982E-4	0.0	0.005370714305002565	8.001064141530824E-5
36-37	2.000266035382706E-4	7.00093112383947E-4	0.0	0.009021199819576003	8.001064141530824E-5
38-39	2.000266035382706E-4	7.500997632685147E-4	0.0	0.01422189151157104	8.001064141530824E-5
40-41	2.000266035382706E-4	8.001064141530824E-4	0.0	0.019932651042588664	8.001064141530824E-5
42-43	2.000266035382706E-4	8.101077443299959E-4	0.0	0.025423381309714194	8.001064141530824E-5
44-45	2.100279337151841E-4	8.601143952145635E-4	0.0	0.0317842273022312	8.001064141530824E-5
46-47	2.2002926389209766E-4	9.001197159222177E-4	0.0	0.037975050681740674	8.001064141530824E-5
48-49	2.2002926389209766E-4	9.201223762760447E-4	0.0	0.04422588204231163	1.000133017691353E-4
50-51	2.3003059406901118E-4	0.001000133017691353	0.0	0.05046671207270567	1.000133017691353E-4
52-53	2.400319242459247E-4	0.001000133017691353	0.0	0.05621747692443095	1.3001729229987588E-4
54-55	2.400319242459247E-4	0.001000133017691353	0.0	0.06224827902110981	1.6002128283061648E-4
56-57	2.400319242459247E-4	0.001040138338399007	0.0	0.06869913698521904	1.6002128283061648E-4
58-59	2.400319242459247E-4	0.001040138338399007	0.0	0.07491996435525924	1.6002128283061648E-4
60-61	2.5003325442283825E-4	0.001040138338399007	0.0	0.0809407651217612	1.6002128283061648E-4
62-63	3.6004788636888704E-4	0.0010501396685759206	0.0	0.08699156987879388	1.8002394318444352E-4
64-65	3.6004788636888704E-4	0.0012201622815834507	0.0	0.09287235202281904	1.8002394318444352E-4
66-67	3.6004788636888704E-4	0.0012501662721141912	0.0	0.09873313150649035	2.000266035382706E-4
68-69	3.6004788636888704E-4	0.0012801702626449318	0.0	0.10387381521742392	2.000266035382706E-4
70-71	3.8005054672271413E-4	0.0013101742531756724	0.0	0.10850443108933489	2.2002926389209766E-4
72-73	4.200558674303682E-4	0.001320175583352586	0.0	0.1125149644902772	2.800372449535788E-4
74-75	4.200558674303682E-4	0.001340178243706413	0.0	0.11592541808060472	2.800372449535788E-4
76-77	4.200558674303682E-4	0.001400186224767894	0.0	0.11916584905792471	2.800372449535788E-4
78-79	4.200558674303682E-4	0.0014301902152986346	0.0	0.12115611376313049	2.800372449535788E-4
80-81	4.200558674303682E-4	0.0014501928756524619	0.0	0.12177619623409913	3.0003990530740586E-4
82-83	4.200558674303682E-4	0.0014601942058293754	0.0	0.1223362707240063	3.300438958381465E-4
84-85	4.200558674303682E-4	0.0014801968661832024	0.0	0.12259630530860605	3.4004522601506E-4
86-87	4.200558674303682E-4	0.001490198196360116	0.0	0.12267631595002135	3.4004522601506E-4
88	4.200558674303682E-4	0.0015602075075985106	0.0	0.12269631861037518	3.8005054672271413E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6780	0.0	47.86821	1
GTATCAA	12440	0.0	37.866627	1
CTTATAC	4385	0.0	37.492085	1
TCAACGC	14345	0.0	32.242554	4
ATCAACG	14395	0.0	32.01222	3
TATCAAC	14615	0.0	31.706766	2
CAACGCA	14795	0.0	31.29066	5
AACGCAG	15475	0.0	29.943214	6
TATACAC	6730	0.0	28.730352	3
ACGCAGA	17465	0.0	26.482647	7
TTATACA	6920	0.0	25.973364	2
CGCAGAG	17855	0.0	25.904198	8
GTGGTAT	3435	0.0	24.178522	1
TGGTATC	3260	0.0	23.386074	2
GATCAAC	2035	0.0	22.394493	1
GCAGAGT	20725	0.0	22.255333	9
TACACAT	9065	0.0	21.658747	5
ACACATC	8720	0.0	20.952747	6
ACATCTC	8870	0.0	20.646431	8
GTACATG	15110	0.0	20.154104	1
>>END_MODULE