FastQCFastQC Report
Fri 27 May 2016
SRR2140113_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140113_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5436895
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75350.050.3138541
GTATCAA134750.037.64991
TCAACGC159000.031.3695744
TATCAAC160250.031.3132462
ATCAACG160500.031.050153
CTTATAC47150.030.6266021
CAACGCA163000.030.5736395
GTGGTAT32650.030.00721
AACGCAG168650.029.5493816
TGGTATC32800.027.792062
TATACAC66900.027.7596023
ACGCAGA198150.025.1286567
CGCAGAG203150.024.5101838
GATCAAC22450.023.338321
TTATACA73650.022.4408742
GCAGAGT238000.020.8675029
GAGTACT134500.020.37863512-13
GTACATG182000.019.9645021
TACACAT91150.019.9068165
TACATGG178200.019.673112
Produced by FastQC (version 0.11.5)