##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140113_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5436895
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.259125475110334	25.0	18.0	33.0	18.0	33.0
2	29.166461923579543	30.0	27.0	32.0	25.0	33.0
3	28.01033494301435	29.0	27.0	31.0	18.0	33.0
4	31.553840381320587	33.0	31.0	33.0	29.0	33.0
5	32.11844131622921	33.0	33.0	33.0	31.0	33.0
6	36.473806832760246	38.0	37.0	38.0	34.0	38.0
7	36.67805870814132	38.0	37.0	38.0	34.0	38.0
8	36.93601090328211	38.0	38.0	38.0	35.0	38.0
9	37.30810508571528	38.0	38.0	38.0	36.0	38.0
10-11	37.31320533135181	38.0	38.0	38.0	36.5	38.0
12-13	37.41854394833816	38.0	38.0	38.0	37.0	38.0
14-15	37.370945364955546	38.0	38.0	38.0	37.0	38.0
16-17	37.456199172505634	38.0	38.0	38.0	37.0	38.0
18-19	37.47418646120626	38.0	38.0	38.0	37.0	38.0
20-21	37.49368224575183	38.0	38.0	38.0	37.0	38.0
22-23	37.511749896114765	38.0	38.0	38.0	37.0	38.0
24-25	37.519120356452206	38.0	38.0	38.0	37.5	38.0
26-27	37.483921327925415	38.0	38.0	38.0	37.0	38.0
28-29	37.46358654036091	38.0	38.0	38.0	37.0	38.0
30-31	37.46678536306524	38.0	38.0	38.0	37.0	38.0
32-33	37.454301543719204	38.0	38.0	38.0	37.0	38.0
34-35	37.42016237963841	38.0	38.0	38.0	37.0	38.0
36-37	37.39488939898573	38.0	38.0	38.0	37.0	38.0
38-39	37.37343679191011	38.0	38.0	38.0	37.0	38.0
40-41	37.38570120014969	38.0	38.0	38.0	37.0	38.0
42-43	37.3487635632991	38.0	38.0	38.0	37.0	38.0
44-45	37.30469598144367	38.0	38.0	38.0	37.0	38.0
46-47	37.26758464844367	38.0	38.0	38.0	37.0	38.0
48-49	37.21687411342326	38.0	38.0	38.0	37.0	38.0
50-51	37.18279107332378	38.0	38.0	38.0	37.0	38.0
52-53	37.148992693903345	38.0	38.0	38.0	37.0	38.0
54-55	37.132756603093625	38.0	38.0	38.0	36.0	38.0
56-57	37.091458214217894	38.0	38.0	38.0	36.0	38.0
58-59	37.05753390325421	38.0	38.0	38.0	36.0	38.0
60-61	37.08052346806416	38.0	38.0	38.0	36.0	38.0
62-63	37.09326998187089	38.0	38.0	38.0	36.0	38.0
64-65	37.10430767223352	38.0	38.0	38.0	36.0	38.0
66-67	37.097919394171	38.0	38.0	38.0	36.0	38.0
68-69	37.06111755436155	38.0	38.0	38.0	36.0	38.0
70-71	37.057560394855884	38.0	38.0	38.0	36.0	38.0
72-73	37.07105213241414	38.0	38.0	38.0	36.0	38.0
74-75	37.086549168684385	38.0	38.0	38.0	36.0	38.0
76-77	37.07087538570099	38.0	38.0	38.0	36.0	38.0
78-79	37.03468617591777	38.0	38.0	38.0	36.0	38.0
80-81	36.990329466947436	38.0	38.0	38.0	36.0	38.0
82-83	36.951452238686485	38.0	38.0	38.0	36.0	38.0
84-85	36.92882474585753	38.0	38.0	38.0	35.5	38.0
86-87	36.95321048456505	38.0	38.0	38.0	36.0	38.0
88-89	36.910614878289365	38.0	38.0	38.0	35.0	38.0
90-91	36.92283826047429	38.0	38.0	38.0	35.0	38.0
92-93	36.91281720900736	38.0	38.0	38.0	35.0	38.0
94-95	36.92945269711941	38.0	38.0	38.0	35.0	38.0
96-97	36.932331559483664	38.0	38.0	38.0	35.0	38.0
98-99	36.91708205136447	38.0	38.0	38.0	35.0	38.0
100	35.39135766834454	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	6.0
20	18.0
21	75.0
22	302.0
23	871.0
24	2130.0
25	4212.0
26	7744.0
27	13364.0
28	21319.0
29	31319.0
30	42002.0
31	55086.0
32	71070.0
33	96456.0
34	149866.0
35	298946.0
36	1036679.0
37	3605429.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.05034859050984	18.523421916369546	11.505445663379557	21.920783829741055
2	14.718046025554058	20.141366934528584	41.73591296964739	23.404674070269976
3	19.30640786156188	25.492506369457214	29.83446032690784	25.366625442073072
4	12.946505674142248	17.80011870829047	38.58731248587189	30.666063131695388
5	13.794472764325963	37.59040776031172	34.82046278252569	13.794656692836629
6	30.230747513056627	37.16945057794936	18.678694365074183	13.921107543919828
7	27.05045802797369	32.71913472671442	22.2296365848522	18.000770660459693
8	24.51505868698954	36.56267042126066	20.91009666362878	18.012174228121015
9	25.642485278821823	17.43736084658615	20.97068271504232	35.94947115954971
10-11	24.485860035921238	26.688459129705468	28.31268582527343	20.512995009099864
12-13	25.43909713172684	24.0761960641138	28.574250560292224	21.91045624386713
14-15	22.581694147118895	25.49765077309751	26.267538733045242	25.653116346738347
16-17	22.050628161846053	28.786835132920537	26.915344144038094	22.247192561195313
18-19	22.086153953681283	27.6419813147026	28.849977790632337	21.42188694098378
20-21	22.882903131823827	26.745556971412764	28.81694707506136	21.55459282170205
22-23	22.56028707579517	26.7847003272074	28.73325726881792	21.92175532817951
24-25	22.404286997732996	26.94202733795269	28.81625060795563	21.837435056358682
26-27	22.31855830517667	27.09788768903415	28.765891820272728	21.817662185516454
28-29	22.121171131379608	27.14618162143062	28.82536273492193	21.90728451226784
30-31	22.746244796727307	26.870601459776296	28.733855036843497	21.649298706652903
32-33	22.083233316140067	27.263805533537163	28.7323024940758	21.920658656246975
34-35	22.342463577149157	27.184304681834913	28.821839822267574	21.651391918748352
36-37	22.231537897196745	27.10506623184848	28.776240737846038	21.887155133108735
38-39	22.07707709016649	27.016817648406015	28.95867993378149	21.947425327646002
40-41	22.36618519070217	26.80780918646554	28.886603082830813	21.939402540001478
42-43	22.385454766922425	26.871529956849184	28.71452786985832	22.028487406370072
44-45	22.28238878884593	27.104289834068005	28.587133803802516	22.026187573283543
46-47	22.529893371299575	26.880215573431382	28.199295869056822	22.39059518621222
48-49	22.371585121115785	27.008813585085058	28.268073045404922	22.35152824839424
50-51	22.248909336052122	27.231990033799512	28.302459487146812	22.21664114300155
52-53	22.493142193619832	27.17164196884958	28.27901205056041	22.056203786970176
54-55	22.402552923776845	27.11238540824193	28.13763401009674	22.347427657884484
56-57	22.54099418566609	27.41680451956057	27.86009820555452	22.18210308921882
58-59	22.289808870596225	27.64430827544553	28.12447555340941	21.94140730054883
60-61	22.49340782886976	27.78093354423939	27.823612713304257	21.902045913586587
62-63	22.304406332692118	27.963225689390043	27.796497035706984	21.935870942210858
64-65	22.26531227699732	27.881193334090458	27.834948019831963	22.018546369080262
66-67	22.42583461904125	27.848731517937807	27.675883849462313	22.049550013558626
68-69	22.336925391350192	27.644088806815052	27.881731196363525	22.13725460547123
70-71	22.371762003492403	27.35677599139982	28.00741614235861	22.26404586274916
72-73	22.553351052970342	27.285184762613223	27.900807559069847	22.260656625346588
74-75	22.289578889525195	27.397624002939757	27.974561011580374	22.338236095954677
76-77	22.515240729110346	27.24277087878671	27.881237209299247	22.360751182803696
78-79	22.439556379056818	27.371801757671033	27.794371120244506	22.39427074302764
80-81	22.52258763841585	27.36774472174524	27.728811437408275	22.380856202430635
82-83	22.513315121333626	27.369242851907682	27.82914685265039	22.288295174108303
84-85	22.635945619454734	27.308776897228455	27.74426513341724	22.311012349899578
86-87	22.415436803281207	27.358533570964184	27.82309686047732	22.402932765277285
88-89	22.541658955246884	27.355671231084848	27.733355962614333	22.36931385105393
90-91	22.552803533222548	27.35116849827614	27.773020880632398	22.32300708786891
92-93	22.599820619894132	27.349451663818776	27.701682209667695	22.349045506619404
94-95	22.461085651553304	27.46119470624302	27.69445057035603	22.383269071847653
96-97	22.559797732119655	27.50570097280351	27.776991191409127	22.157510103667704
98-99	23.618618744565246	29.51312456154156	27.916852901245143	18.95140379264805
100	22.603963532683306	30.344580942659015	21.854989615966772	25.196465908690907
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	735.0
1	765.5
2	1103.0
3	1573.0
4	2046.0
5	2859.5
6	3689.5
7	4666.0
8	6306.0
9	7447.5
10	7331.5
11	7153.0
12	7804.0
13	9488.5
14	12627.5
15	16274.5
16	19498.5
17	22255.0
18	22955.0
19	21328.5
20	18994.0
21	17187.5
22	16905.0
23	18740.0
24	23048.0
25	29746.0
26	39135.0
27	49958.0
28	60030.0
29	70431.5
30	80724.0
31	91206.5
32	103655.0
33	116447.5
34	129310.0
35	141397.5
36	154087.5
37	167132.0
38	176360.0
39	183433.0
40	192901.5
41	202049.5
42	208692.5
43	220283.0
44	234442.5
45	242785.0
46	242167.0
47	238381.0
48	235358.0
49	230217.5
50	225539.5
51	220311.5
52	211255.0
53	200667.5
54	190688.0
55	178953.5
56	165153.5
57	149387.5
58	133360.5
59	117567.0
60	99404.0
61	80752.0
62	63425.0
63	48784.0
64	37395.0
65	27772.0
66	20320.5
67	15483.0
68	11846.0
69	8640.0
70	6152.0
71	4400.0
72	3070.0
73	2043.0
74	1337.0
75	855.0
76	564.5
77	369.0
78	236.0
79	166.5
80	116.0
81	68.5
82	42.5
83	33.5
84	29.0
85	20.0
86	15.5
87	13.5
88	7.5
89	3.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00483731983052827
3	2.0232136173312158E-4
4	6.989283405326018E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	9.196901710964004E-6
24-25	1.287650202096699E-4
26-27	7.818679487583983E-4
28-29	0.0012698321557940509
30-31	7.092233856555979E-4
32-33	7.013843943633428E-4
34-35	0.001036286784595486
36-37	0.0
38-39	2.148248780285054E-4
40-41	7.048461461832111E-4
42-43	2.9316896452816297E-4
44-45	0.0
46-47	0.0
48-49	0.001483159020141784
50-51	0.003654713246215002
52-53	0.0
54-55	0.003967830024510278
56-57	0.03960430102709154
58-59	0.05709579385241388
60-61	0.03073298630118591
62-63	0.006834525065158884
64-65	0.0
66-67	0.0
68-69	8.459275198726879E-5
70-71	0.00376548354531829
72-73	0.008269538462337772
74-75	0.006053101433262972
76-77	0.007236347378649708
78-79	0.010428871752063824
80-81	0.011515971233711739
82-83	0.04421532636494634
84-85	0.026138542544267847
86-87	0.0012063119344035407
88-89	0.0016526290867407577
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	33.0
20-21	165.0
22-23	375.0
24-25	426.0
26-27	1378.0
28-29	3649.0
30-31	10112.0
32-33	12939.0
34-35	17801.0
36-37	28472.0
38-39	33251.0
40-41	32628.0
42-43	37006.0
44-45	44123.0
46-47	45732.0
48-49	42284.0
50-51	38821.0
52-53	37314.0
54-55	40342.0
56-57	42151.0
58-59	41937.0
60-61	43400.0
62-63	41794.0
64-65	47335.0
66-67	51612.0
68-69	52333.0
70-71	58920.0
72-73	43877.0
74-75	42981.0
76-77	44684.0
78-79	45900.0
80-81	45630.0
82-83	43102.0
84-85	42884.0
86-87	44314.0
88-89	45693.0
90-91	46872.0
92-93	45852.0
94-95	53540.0
96-97	167856.0
98-99	366252.0
100-101	3551125.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.8374929110943
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.48277271639856	80.3561739996391
2	6.195768438705455	10.884415326271444
3	1.3554342498060117	3.571738389263699
4	0.455521618253646	1.6004750765681932
5	0.2064412786213764	0.9066642173731196
6	0.10516260988758225	0.5542332000307608
7	0.06339517316229144	0.38979311512682585
8	0.034528078197479224	0.24262878591238324
9	0.02551815562310741	0.20173057322840088
>10	0.07314698056573	0.9903033713731851
>50	0.001415073686112083	0.0848513199945383
>100	7.744304766546064E-4	0.1304172295817692
>500	9.089746479456309E-5	0.05208363237325691
>1k	3.02991512868575E-5	0.03449176326337489
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.8392851066647416E-5	0.0	0.0	0.0
3	0.0	1.8392851066647416E-5	0.0	0.0	0.0
4	0.0	3.678570213329483E-5	0.0	1.8392851066647416E-5	0.0
5	1.8392851066647416E-5	3.678570213329483E-5	0.0	1.8392851066647416E-5	0.0
6	1.8392851066647416E-5	3.678570213329483E-5	0.0	1.8392851066647416E-5	1.8392851066647416E-5
7	1.8392851066647416E-5	5.517855319994225E-5	0.0	1.8392851066647416E-5	1.8392851066647416E-5
8	1.8392851066647416E-5	5.517855319994225E-5	0.0	1.8392851066647416E-5	5.517855319994225E-5
9	1.8392851066647416E-5	1.2874995746653192E-4	0.0	5.517855319994225E-5	5.517855319994225E-5
10-11	5.517855319994225E-5	1.2874995746653192E-4	0.0	5.517855319994225E-5	5.517855319994225E-5
12-13	5.517855319994225E-5	1.2874995746653192E-4	0.0	5.517855319994225E-5	5.517855319994225E-5
14-15	6.437497873326596E-5	1.9312493619979786E-4	0.0	5.517855319994225E-5	9.196425533323707E-5
16-17	7.357140426658966E-5	2.0232136173312158E-4	0.0	7.357140426658966E-5	1.4714280853317933E-4
18-19	7.357140426658966E-5	2.1151778726644527E-4	0.0	7.357140426658966E-5	1.6553565959982674E-4
20-21	7.357140426658966E-5	2.20714212799769E-4	0.0	7.357140426658966E-5	1.6553565959982674E-4
22-23	7.357140426658966E-5	2.391070638664164E-4	0.0	7.357140426658966E-5	1.6553565959982674E-4
24-25	8.276782979991337E-5	2.7589276599971127E-4	0.0	9.196425533323707E-5	1.6553565959982674E-4
26-27	1.0116068086656079E-4	2.9428561706635865E-4	0.0	2.299106383330927E-4	1.6553565959982674E-4
28-29	1.103571063998845E-4	2.9428561706635865E-4	0.0	5.241962553994514E-4	1.6553565959982674E-4
30-31	1.103571063998845E-4	3.494641702663009E-4	0.0	0.0016185708938649727	1.7473208513315045E-4
32-33	1.2874995746653192E-4	3.494641702663009E-4	0.0	0.0044418735325953505	1.8392851066647415E-4
34-35	1.3794638299985564E-4	4.3223200006621424E-4	0.0	0.007743390299058562	2.0232136173312158E-4
36-37	1.4714280853317933E-4	5.05803404332804E-4	0.0	0.012507138725320242	2.1151778726644527E-4
38-39	1.6553565959982674E-4	5.149998298661277E-4	0.0	0.019818297024312592	2.483034893997401E-4
40-41	1.8392851066647415E-4	5.425891064660987E-4	0.0	0.02803070502557066	2.5749991493306384E-4
42-43	1.8392851066647415E-4	5.793748085993936E-4	0.0	0.03617873804809547	2.5749991493306384E-4
44-45	1.9312493619979786E-4	6.345533617993359E-4	0.0	0.04533837787928588	2.5749991493306384E-4
46-47	2.0232136173312158E-4	6.89731914999278E-4	0.0	0.05451641056154294	2.7589276599971127E-4
48-49	2.0232136173312158E-4	6.989283405326018E-4	0.0	0.06382319320126653	2.9428561706635865E-4
50-51	2.0232136173312158E-4	8.276782979991337E-4	0.0	0.07263336886219066	3.0348204259968235E-4
52-53	2.20714212799769E-4	8.552675745991048E-4	0.0	0.08152631235291467	3.310713191996535E-4
54-55	2.20714212799769E-4	8.920532767323997E-4	0.0	0.08947202401370635	3.586605957996246E-4
56-57	2.20714212799769E-4	9.196425533323707E-4	0.0	0.09536693278056685	3.678570213329483E-4
58-59	2.20714212799769E-4	9.196425533323707E-4	0.0	0.1015561271644937	3.77053446866272E-4
60-61	2.20714212799769E-4	9.196425533323707E-4	0.0	0.10754300018668744	3.8624987239959573E-4
62-63	2.20714212799769E-4	9.288389788656944E-4	0.0	0.11366781959188103	3.8624987239959573E-4
64-65	2.20714212799769E-4	0.0010116068086656077	0.0	0.11947076410340829	3.8624987239959573E-4
66-67	2.20714212799769E-4	0.0010116068086656077	0.0	0.12558638708306855	3.954462979329195E-4
68-69	2.20714212799769E-4	0.0010208032341989315	0.0	0.13085593891366304	4.0464272346624316E-4
70-71	2.20714212799769E-4	0.0010299996597322553	0.0	0.13538977670159164	4.2303557453289055E-4
72-73	2.20714212799769E-4	0.001039196085265579	0.0	0.1393810253830541	4.2303557453289055E-4
74-75	2.20714212799769E-4	0.0010575889363322265	0.0	0.142673345723984	4.2303557453289055E-4
76-77	2.20714212799769E-4	0.0010759817873988737	0.0	0.14568057687338087	4.2303557453289055E-4
78-79	2.20714212799769E-4	0.0010851782129321975	0.0	0.1473727191715124	4.2303557453289055E-4
80-81	2.20714212799769E-4	0.0010943746384655213	0.0	0.14792450470351184	4.506248511328617E-4
82-83	2.20714212799769E-4	0.0011035710639988449	0.0	0.14831995100144477	4.966069787994802E-4
84-85	2.20714212799769E-4	0.0011035710639988449	0.0	0.14844870095891127	4.966069787994802E-4
86-87	2.20714212799769E-4	0.0011587496171987872	0.0	0.1486050401929778	5.149998298661277E-4
88	2.20714212799769E-4	0.001195535319332082	0.0	0.14863262946957775	5.149998298661277E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7535	0.0	50.313854	1
GTATCAA	13475	0.0	37.6499	1
TCAACGC	15900	0.0	31.369574	4
TATCAAC	16025	0.0	31.313246	2
ATCAACG	16050	0.0	31.05015	3
CTTATAC	4715	0.0	30.626602	1
CAACGCA	16300	0.0	30.573639	5
GTGGTAT	3265	0.0	30.0072	1
AACGCAG	16865	0.0	29.549381	6
TGGTATC	3280	0.0	27.79206	2
TATACAC	6690	0.0	27.759602	3
ACGCAGA	19815	0.0	25.128656	7
CGCAGAG	20315	0.0	24.510183	8
GATCAAC	2245	0.0	23.33832	1
TTATACA	7365	0.0	22.440874	2
GCAGAGT	23800	0.0	20.867502	9
GAGTACT	13450	0.0	20.378635	12-13
GTACATG	18200	0.0	19.964502	1
TACACAT	9115	0.0	19.906816	5
TACATGG	17820	0.0	19.67311	2
>>END_MODULE