FastQCFastQC Report
Fri 27 May 2016
SRR2140112_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140112_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5631146
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75350.047.0000041
GTATCAA129550.035.7452621
TCAACGC144550.031.1463184
ATCAACG144800.031.092823
CTTATAC49550.031.037411
TATCAAC148850.030.507812
CAACGCA150150.029.9557065
AACGCAG156450.028.831346
TATACAC72200.026.3275763
ACGCAGA178350.025.2910757
CGCAGAG181050.024.913918
GATCAAC25400.022.8734591
TTATACA76950.022.4839732
GCAGAGT217050.020.7629
GTGGTAT39800.020.7157611
GTACATG165500.020.4176221
GAGTACT122350.020.31868212-13
TACATGG159400.020.2073652
TGGTATC36900.020.0284062
ACATCTC91200.019.7648778
Produced by FastQC (version 0.11.5)