##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140112_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5631146
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.25141436574367	25.0	18.0	33.0	18.0	33.0
2	29.320070195303053	31.0	27.0	33.0	25.0	33.0
3	28.171381278340146	29.0	27.0	31.0	18.0	33.0
4	31.578284953009565	33.0	31.0	33.0	29.0	33.0
5	32.12805723737229	33.0	33.0	33.0	31.0	33.0
6	36.51981745811599	38.0	37.0	38.0	34.0	38.0
7	36.6470618946836	38.0	37.0	38.0	34.0	38.0
8	36.94060143352703	38.0	38.0	38.0	35.0	38.0
9	37.302686167256184	38.0	38.0	38.0	36.0	38.0
10-11	37.3061421245338	38.0	38.0	38.0	36.5	38.0
12-13	37.42067440623987	38.0	38.0	38.0	37.0	38.0
14-15	37.37611606944661	38.0	38.0	38.0	37.0	38.0
16-17	37.46566089744432	38.0	38.0	38.0	37.0	38.0
18-19	37.47864644248258	38.0	38.0	38.0	37.0	38.0
20-21	37.49745762713141	38.0	38.0	38.0	37.0	38.0
22-23	37.51753796586455	38.0	38.0	38.0	37.5	38.0
24-25	37.527930448796766	38.0	38.0	38.0	37.5	38.0
26-27	37.488847338861675	38.0	38.0	38.0	37.0	38.0
28-29	37.46113699040238	38.0	38.0	38.0	37.0	38.0
30-31	37.472156822429525	38.0	38.0	38.0	37.0	38.0
32-33	37.46233221201632	38.0	38.0	38.0	37.0	38.0
34-35	37.42895734356337	38.0	38.0	38.0	37.0	38.0
36-37	37.40274146985983	38.0	38.0	38.0	37.0	38.0
38-39	37.38458965777391	38.0	38.0	38.0	37.0	38.0
40-41	37.40621511151403	38.0	38.0	38.0	37.0	38.0
42-43	37.37082950936192	38.0	38.0	38.0	37.0	38.0
44-45	37.334385256414535	38.0	38.0	38.0	37.0	38.0
46-47	37.30826872507893	38.0	38.0	38.0	37.0	38.0
48-49	37.26511271405895	38.0	38.0	38.0	37.0	38.0
50-51	37.23420418352849	38.0	38.0	38.0	37.0	38.0
52-53	37.203142144805206	38.0	38.0	38.0	37.0	38.0
54-55	37.185006255824234	38.0	38.0	38.0	37.0	38.0
56-57	37.14471269201047	38.0	38.0	38.0	36.5	38.0
58-59	37.113312836346424	38.0	38.0	38.0	36.0	38.0
60-61	37.13358976910977	38.0	38.0	38.0	36.0	38.0
62-63	37.146411240051336	38.0	38.0	38.0	36.0	38.0
64-65	37.151584718586065	38.0	38.0	38.0	36.0	38.0
66-67	37.14656347122823	38.0	38.0	38.0	36.0	38.0
68-69	37.108002166472886	38.0	38.0	38.0	36.0	38.0
70-71	37.107317921763375	38.0	38.0	38.0	36.0	38.0
72-73	37.117529050027734	38.0	38.0	38.0	36.0	38.0
74-75	37.13449924482882	38.0	38.0	38.0	36.0	38.0
76-77	37.119154840592856	38.0	38.0	38.0	36.0	38.0
78-79	37.08264755329885	38.0	38.0	38.0	36.0	38.0
80-81	37.04028660650093	38.0	38.0	38.0	36.0	38.0
82-83	37.0050321242256	38.0	38.0	38.0	36.0	38.0
84-85	36.98168727461611	38.0	38.0	38.0	36.0	38.0
86-87	37.00277852614272	38.0	38.0	38.0	36.0	38.0
88-89	36.96309593822267	38.0	38.0	38.0	36.0	38.0
90-91	36.978282149682414	38.0	38.0	38.0	36.0	38.0
92-93	36.964151178669916	38.0	38.0	38.0	36.0	38.0
94-95	36.98065019193534	38.0	38.0	38.0	36.0	38.0
96-97	36.9812701578591	38.0	38.0	38.0	36.0	38.0
98-99	36.96360734200579	38.0	38.0	38.0	35.0	38.0
100	35.45407515415067	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	7.0
20	24.0
21	76.0
22	311.0
23	882.0
24	2112.0
25	4323.0
26	7927.0
27	13149.0
28	20888.0
29	30577.0
30	40909.0
31	53453.0
32	69432.0
33	96362.0
34	150499.0
35	304634.0
36	1053475.0
37	3782101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.55866745419139	18.970312614874484	11.17115414872923	22.2998657822049
2	14.483067512750846	20.29774438921056	41.999480358307686	23.219707739730904
3	19.19485219689086	25.5176038954812	29.927510237713857	25.360033669914085
4	12.811280653912243	17.95882574818525	38.82593287412104	30.403960723781466
5	13.726726318230783	37.53452671978315	35.12336565239118	13.615381309594884
6	30.13883852416542	37.50858173451727	18.697615014776744	13.654964726540566
7	26.886196877154312	32.706468630008885	22.37937002521334	18.027964467623462
8	24.21611870834107	36.992043893019286	21.009275909379724	17.782561489259912
9	25.281621183325736	17.713623479128405	21.18250885343765	35.822246484108206
10-11	24.23520008183059	26.90874113368753	28.54012309394926	20.31593569053262
12-13	25.149525513989513	24.205410408467475	28.75622475425073	21.888839323292274
14-15	22.294351806896856	25.7897859512078	26.341636320564234	25.574225921331113
16-17	21.950966996771175	29.059271416510956	27.020201571758218	21.969560014959654
18-19	21.927481546385053	27.97520256089968	28.751731885481213	21.34558400723405
20-21	22.6828524380991	27.113913811344275	28.725070823479165	21.478162927077456
22-23	22.289056169107763	27.18700179776806	28.621833105393463	21.90210892773072
24-25	22.183531250485636	27.269871081895296	28.792201420385798	21.754396247233267
26-27	22.137651491064624	27.48536822342257	28.711968482398348	21.66501180311446
28-29	21.818228499990404	27.357399835537887	28.871824740531526	21.952546923940183
30-31	22.381954579131897	27.102980004190023	28.945017124682415	21.570048291995665
32-33	21.97844648710811	27.51373755361339	28.71280116019273	21.795014799085774
34-35	22.064545390723765	27.528477667759976	28.90907314295167	21.49790379856459
36-37	22.30308308603931	27.28924600718666	28.68104087539528	21.72663003137875
38-39	21.940527160052163	27.33303016052531	28.86090030062433	21.865542378798196
40-41	22.262400110016976	27.16922267761261	28.773912115048294	21.794465097322117
42-43	22.277261658818315	27.189561801478856	28.707912105626303	21.825264434076526
44-45	22.163519706286557	27.44407036749673	28.562800944005357	21.82960898221135
46-47	22.422284832778256	27.213652216907004	28.129090503781303	22.23497244653344
48-49	22.257186171669158	27.28591664459795	28.30015171305585	22.156745470677038
50-51	22.110122351885604	27.584167579294995	28.28108889596791	22.024621172851493
52-53	22.29259595241196	27.585561372445007	28.275493740504626	21.846348934638407
54-55	22.260915938019323	27.533707271987197	28.089249863043886	22.116126926949597
56-57	22.421008519665346	27.838130698523827	27.821001671912594	21.919859109898233
58-59	22.08695696158795	28.0644601492654	28.126511398792164	21.72207149035448
60-61	22.338649989914522	28.21697358703219	27.779962310916883	21.664414112136402
62-63	22.12970573144312	28.4022129527574	27.732380626607377	21.7357006891921
64-65	22.099369512589877	28.368029477727656	27.761664855793455	21.77093615388901
66-67	22.318596613520402	28.30581323242008	27.597149329613224	21.778440824446292
68-69	22.211692631630903	28.142628148420386	27.76277767177937	21.882901548169343
70-71	22.236527064111993	27.78150817972595	27.935333952254478	22.04663080390758
72-73	22.411298501923294	27.66102406245579	27.91575817589495	22.01191925972596
74-75	22.183820026117193	27.78392859137214	27.91399828006675	22.11825310244392
76-77	22.2826394475745	27.65147051253198	27.89013178858859	22.17575825130493
78-79	22.289016978414917	27.769742641458684	27.747108908538447	22.19413147158795
80-81	22.349598590263852	27.688742013640272	27.75701281442171	22.20464658167417
82-83	22.361221049039226	27.637835842550025	27.911425012682617	22.089518095728135
84-85	22.523658174420163	27.58031202352067	27.81219955369539	22.08383024836378
86-87	22.273048349556866	27.6093259491184	27.94250095771746	22.17512474360727
88-89	22.39095133650694	27.647829327579316	27.798467951991267	22.162751383922476
90-91	22.421229806510603	27.664903506484436	27.81935889595909	22.09450779104588
92-93	22.403297842932325	27.65581563382078	27.802459531535938	22.138426991710954
94-95	22.293194724336278	27.743594208730975	27.80392479090884	22.15928627602391
96-97	22.37943555678076	27.825278116190766	27.899039302345574	21.896247024682904
98-99	23.44498808918871	29.76603777258542	28.072277751639508	18.716696386586364
100	22.443996680643426	30.608320524083872	21.999314374832416	24.94836842044029
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	612.0
1	651.5
2	861.0
3	1149.5
4	1609.5
5	2419.0
6	3099.5
7	3733.0
8	4689.5
9	5427.5
10	5537.0
11	5588.5
12	6275.5
13	7903.0
14	10750.0
15	14204.5
16	17501.5
17	20373.5
18	21389.0
19	20112.5
20	18149.0
21	16546.5
22	16519.5
23	18816.5
24	23582.5
25	30823.5
26	40882.0
27	52628.0
28	63801.5
29	75817.5
30	87744.0
31	99116.0
32	112265.0
33	125870.5
34	140168.5
35	154307.0
36	169514.0
37	183912.0
38	193727.5
39	201848.0
40	211446.0
41	220631.0
42	228487.5
43	240744.5
44	253324.5
45	259933.0
46	258071.0
47	252429.0
48	246453.0
49	239015.0
50	232440.5
51	224586.0
52	212953.5
53	199608.0
54	187494.5
55	173097.5
56	157161.5
57	141847.5
58	126292.5
59	110300.0
60	92955.5
61	75224.5
62	58772.5
63	44883.0
64	33678.0
65	24963.5
66	18430.5
67	14012.5
68	10592.0
69	7618.5
70	5478.5
71	3862.0
72	2636.5
73	1777.5
74	1234.0
75	814.0
76	499.5
77	290.5
78	180.0
79	106.5
80	63.5
81	43.5
82	31.0
83	22.0
84	16.5
85	13.5
86	10.5
87	6.5
88	4.0
89	3.0
90	2.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006091122481995672
3	1.0655024749846657E-4
4	9.58952227486199E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.775923871116232E-5
24-25	5.328108944909218E-5
26-27	7.726584076291759E-4
28-29	0.001519154809781829
30-31	8.803243506162936E-4
32-33	7.750951072878629E-4
34-35	8.842392394113469E-4
36-37	0.0
38-39	1.2622166796885355E-4
40-41	9.07123852250207E-4
42-43	3.9249978549430327E-4
44-45	0.0
46-47	0.0
48-49	0.0018514933977428613
50-51	0.003920535273696714
52-53	0.0
54-55	0.004150592683595347
56-57	0.03969156080482955
58-59	0.05767952911416279
60-61	0.03167877292714412
62-63	0.007313817087075891
64-65	0.0
66-67	0.0
68-69	2.039519982655922E-5
70-71	0.0035697778071246988
72-73	0.008674823157831883
74-75	0.006100101721039925
76-77	0.007125614371534639
78-79	0.01096654344969219
80-81	0.011925077526567478
82-83	0.04432836930135464
84-85	0.02651970294390839
86-87	0.001307866672717879
88-89	0.0016490122585990062
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	39.0
20-21	151.0
22-23	367.0
24-25	462.0
26-27	1290.0
28-29	3486.0
30-31	10221.0
32-33	13006.0
34-35	18548.0
36-37	29134.0
38-39	34330.0
40-41	33504.0
42-43	37981.0
44-45	45039.0
46-47	45707.0
48-49	42641.0
50-51	38818.0
52-53	38039.0
54-55	41431.0
56-57	43093.0
58-59	43766.0
60-61	47029.0
62-63	43941.0
64-65	48382.0
66-67	53965.0
68-69	54465.0
70-71	61230.0
72-73	44564.0
74-75	43898.0
76-77	45756.0
78-79	46977.0
80-81	47174.0
82-83	44377.0
84-85	44029.0
86-87	45659.0
88-89	47314.0
90-91	47781.0
92-93	46965.0
94-95	54884.0
96-97	170975.0
98-99	374831.0
100-101	3695897.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.69971956298325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.16678934679481	79.95301859171474
2	6.441371081765866	11.298128749439531
3	1.4247874310444049	3.7486037441837294
4	0.4731530239119213	1.6598155002981205
5	0.20135736449603797	0.8829492199121965
6	0.10177251942557733	0.5355252847704838
7	0.057096123931717736	0.35051198398614875
8	0.03991972187776519	0.28007587309698356
9	0.024791989959142217	0.19568255101425616
>10	0.06710202853578615	0.8868667836390034
>50	0.0010796031911083433	0.06229761351308639
>100	7.505180081448967E-4	0.12561969049573507
>500	2.9247057777831828E-5	0.02090441393603949
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.775837458307776E-5	0.0	1.775837458307776E-5	0.0
5	0.0	1.775837458307776E-5	0.0	1.775837458307776E-5	0.0
6	0.0	1.775837458307776E-5	0.0	1.775837458307776E-5	0.0
7	0.0	1.775837458307776E-5	0.0	3.551674916615552E-5	0.0
8	1.775837458307776E-5	1.775837458307776E-5	0.0	3.551674916615552E-5	0.0
9	1.775837458307776E-5	3.551674916615552E-5	0.0	5.327512374923328E-5	0.0
10-11	1.775837458307776E-5	3.551674916615552E-5	0.0	7.103349833231104E-5	0.0
12-13	1.775837458307776E-5	5.327512374923328E-5	0.0	1.0655024749846656E-4	0.0
14-15	1.775837458307776E-5	1.0655024749846656E-4	0.0	1.0655024749846656E-4	0.0
16-17	1.775837458307776E-5	1.2430862208154432E-4	0.0	1.0655024749846656E-4	0.0
18-19	1.775837458307776E-5	1.4206699666462208E-4	0.0	1.1542943479000544E-4	0.0
20-21	1.775837458307776E-5	1.5982537124769984E-4	0.0	1.331878093730832E-4	0.0
22-23	1.775837458307776E-5	1.775837458307776E-4	0.0	1.5982537124769984E-4	0.0
24-25	1.775837458307776E-5	1.9534212041385536E-4	0.0	1.775837458307776E-4	0.0
26-27	1.775837458307776E-5	2.1310049499693312E-4	0.0	2.3973805687154976E-4	0.0
28-29	1.775837458307776E-5	2.21979682288472E-4	0.0	5.860263612415661E-4	0.0
30-31	1.775837458307776E-5	2.663756187461664E-4	0.0	0.001553857776019304	0.0
32-33	2.663756187461664E-5	2.663756187461664E-4	0.0	0.0036493459768224797	0.0
34-35	3.551674916615552E-5	2.9301318062078304E-4	0.0	0.006517323471989538	0.0
36-37	3.551674916615552E-5	3.196507424953997E-4	0.0	0.010175548636103557	0.0
38-39	3.551674916615552E-5	3.3740911707847744E-4	0.0	0.016053570623102295	0.0
40-41	5.327512374923328E-5	3.995634281192496E-4	0.0	0.022162451479681045	0.0
42-43	7.103349833231104E-5	4.2620098999386624E-4	0.0	0.02863537901521289	0.0
44-45	9.767106020692768E-5	4.7059692645156064E-4	0.0	0.0356499369755286	0.0
46-47	1.2430862208154432E-4	4.972344883261773E-4	0.0	0.04326827967166896	0.0
48-49	1.2430862208154432E-4	4.972344883261773E-4	0.0	0.05066464268552085	0.0
50-51	1.2430862208154432E-4	5.860263612415661E-4	0.0	0.05788342195354196	0.0
52-53	1.2430862208154432E-4	5.860263612415661E-4	0.0	0.06492461747573229	0.0
54-55	1.331878093730832E-4	6.215431104077216E-4	0.0	0.07228546374041803	0.0
56-57	1.5982537124769984E-4	6.925766087400326E-4	0.0	0.07940657194823221	0.0
58-59	1.5982537124769984E-4	7.014557960315715E-4	0.0	0.08611923754063561	0.0
60-61	1.5982537124769984E-4	7.36972545197727E-4	0.0	0.09279638638387283	0.0
62-63	1.5982537124769984E-4	7.636101070723437E-4	0.0	0.09931370985586238	0.0
64-65	1.5982537124769984E-4	8.16885230821577E-4	0.0	0.10566232876931267	0.0
66-67	1.5982537124769984E-4	8.16885230821577E-4	0.0	0.11148707563256219	0.0
68-69	1.5982537124769984E-4	8.346436054046547E-4	0.0	0.11755156055268323	0.0
70-71	1.775837458307776E-4	8.701603545708102E-4	0.0	0.12242623437573807	0.0
72-73	1.775837458307776E-4	9.056771037369658E-4	0.0	0.12590687579402132	0.0
74-75	1.775837458307776E-4	9.234354783200435E-4	0.0	0.12888140353668684	0.0
76-77	1.775837458307776E-4	9.500730401946602E-4	0.0	0.1317937769683116	0.0
78-79	1.775837458307776E-4	9.58952227486199E-4	0.0	0.13351633930287016	0.0
80-81	1.775837458307776E-4	9.58952227486199E-4	0.0	0.13405796972765402	0.0
82-83	1.775837458307776E-4	9.678314147777379E-4	0.0	0.13473278796181098	0.0
84-85	1.775837458307776E-4	9.944689766523546E-4	0.0	0.1349902843932656	0.0
86-87	1.775837458307776E-4	0.0010122273512354323	0.0	0.13509683464076405	0.0
88	1.775837458307776E-4	0.0010122273512354323	0.0	0.1351057138280556	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7535	0.0	47.000004	1
GTATCAA	12955	0.0	35.745262	1
TCAACGC	14455	0.0	31.146318	4
ATCAACG	14480	0.0	31.09282	3
CTTATAC	4955	0.0	31.03741	1
TATCAAC	14885	0.0	30.50781	2
CAACGCA	15015	0.0	29.955706	5
AACGCAG	15645	0.0	28.83134	6
TATACAC	7220	0.0	26.327576	3
ACGCAGA	17835	0.0	25.291075	7
CGCAGAG	18105	0.0	24.91391	8
GATCAAC	2540	0.0	22.873459	1
TTATACA	7695	0.0	22.483973	2
GCAGAGT	21705	0.0	20.762	9
GTGGTAT	3980	0.0	20.715761	1
GTACATG	16550	0.0	20.417622	1
GAGTACT	12235	0.0	20.318682	12-13
TACATGG	15940	0.0	20.207365	2
TGGTATC	3690	0.0	20.028406	2
ACATCTC	9120	0.0	19.764877	8
>>END_MODULE