FastQCFastQC Report
Fri 27 May 2016
SRR2140111_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140111_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5584097
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72300.046.6580851
GTATCAA127100.036.4349821
TCAACGC139700.032.285984
ATCAACG141100.031.9656353
CAACGCA144650.031.1509085
TATCAAC146150.031.0104272
AACGCAG148850.030.2431146
ACGCAGA174600.025.7582827
CGCAGAG176150.025.5316268
GCAGAGT212100.021.2243639
GTACATG163950.021.0206811
TACATGG159850.020.7275182
GTGGTAT38950.020.60521
TGGTATC36700.020.1143042
GAGTACT123850.020.07970412-13
ACATGGG161800.019.8129793
CAGAGTA204400.019.02160510-11
GATCAAC22450.018.1614251
CATGGGG112300.018.113664
GTACTTT137050.018.00497814-15
Produced by FastQC (version 0.11.5)