##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140111_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5584097
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.202269229922045	25.0	18.0	33.0	18.0	33.0
2	29.040801404416865	30.0	27.0	32.0	25.0	33.0
3	28.03848016966754	29.0	27.0	31.0	18.0	33.0
4	31.58774319285643	33.0	31.0	33.0	29.0	33.0
5	32.14731029923012	33.0	33.0	33.0	31.0	33.0
6	36.49206433914024	38.0	37.0	38.0	34.0	38.0
7	36.72789387433635	38.0	37.0	38.0	34.0	38.0
8	36.94570366524793	38.0	38.0	38.0	35.0	38.0
9	37.29998655109322	38.0	38.0	38.0	36.0	38.0
10-11	37.30628004850202	38.0	38.0	38.0	36.5	38.0
12-13	37.409074824452375	38.0	38.0	38.0	37.0	38.0
14-15	37.35182608754826	38.0	38.0	38.0	37.0	38.0
16-17	37.43886683916844	38.0	38.0	38.0	37.0	38.0
18-19	37.455875408324744	38.0	38.0	38.0	37.0	38.0
20-21	37.476193186832845	38.0	38.0	38.0	37.0	38.0
22-23	37.491473579348515	38.0	38.0	38.0	37.0	38.0
24-25	37.498798803186574	38.0	38.0	38.0	37.0	38.0
26-27	37.46747804849304	38.0	38.0	38.0	37.0	38.0
28-29	37.44562690972025	38.0	38.0	38.0	37.0	38.0
30-31	37.44940949099802	38.0	38.0	38.0	37.0	38.0
32-33	37.442679301652774	38.0	38.0	38.0	37.0	38.0
34-35	37.413871293571525	38.0	38.0	38.0	37.0	38.0
36-37	37.39025941589298	38.0	38.0	38.0	37.0	38.0
38-39	37.37933563544648	38.0	38.0	38.0	37.0	38.0
40-41	37.37382605443509	38.0	38.0	38.0	37.0	38.0
42-43	37.3350607976486	38.0	38.0	38.0	37.0	38.0
44-45	37.291173057785535	38.0	38.0	38.0	37.0	38.0
46-47	37.2604196828065	38.0	38.0	38.0	37.0	38.0
48-49	37.21390417016124	38.0	38.0	38.0	37.0	38.0
50-51	37.17635537600358	38.0	38.0	38.0	37.0	38.0
52-53	37.14040003057836	38.0	38.0	38.0	36.0	38.0
54-55	37.119349359822294	38.0	38.0	38.0	36.0	38.0
56-57	37.072050682744695	38.0	38.0	38.0	36.0	38.0
58-59	37.03826368038732	38.0	38.0	38.0	36.0	38.0
60-61	37.05969202756705	38.0	38.0	38.0	36.0	38.0
62-63	37.06793560886788	38.0	38.0	38.0	36.0	38.0
64-65	37.071810935313636	38.0	38.0	38.0	36.0	38.0
66-67	37.06182586160477	38.0	38.0	38.0	36.0	38.0
68-69	37.020623220329334	38.0	38.0	38.0	36.0	38.0
70-71	37.01265436190268	38.0	38.0	38.0	36.0	38.0
72-73	37.02295188758298	38.0	38.0	38.0	36.0	38.0
74-75	37.03693974224517	38.0	38.0	38.0	36.0	38.0
76-77	37.0119041163729	38.0	38.0	38.0	36.0	38.0
78-79	36.96773547825951	38.0	38.0	38.0	36.0	38.0
80-81	36.91627191480775	38.0	38.0	38.0	35.5	38.0
82-83	36.872696570554346	38.0	38.0	38.0	35.0	38.0
84-85	36.844424329614625	38.0	38.0	38.0	35.0	38.0
86-87	36.862876535467265	38.0	38.0	38.0	35.0	38.0
88-89	36.81503822143569	38.0	38.0	38.0	35.0	38.0
90-91	36.8252792898092	38.0	38.0	38.0	35.0	38.0
92-93	36.808445074423815	38.0	38.0	38.0	35.0	38.0
94-95	36.82278369688672	38.0	38.0	38.0	35.0	38.0
96-97	36.822182893259196	38.0	38.0	38.0	35.0	38.0
98-99	36.799588089591154	38.0	38.0	38.0	35.0	38.0
100	35.1522755671965	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	8.0
20	19.0
21	89.0
22	313.0
23	919.0
24	2334.0
25	4738.0
26	8651.0
27	14209.0
28	22359.0
29	32825.0
30	44288.0
31	57032.0
32	74637.0
33	102906.0
34	159171.0
35	313121.0
36	1082166.0
37	3664311.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.6615646182364	18.649479047373283	11.908317495201103	21.78063883918922
2	14.528688146592984	20.5431546726263	41.85849137870051	23.0696658020802
3	19.34000622841745	25.499800056696863	29.769614814106454	25.390578900779232
4	12.786785371344905	17.92864109122154	39.35303992404085	29.93153361339271
5	13.755652883536944	37.618365153757175	35.113161537129464	13.512820425576418
6	29.99627334553823	37.62363010527933	19.054737050592067	13.325359498590373
7	26.88354088404983	32.682258205758245	22.838983635133843	17.59521727505808
8	23.973276252185446	37.14403600080729	21.21479981454477	17.66788793246249
9	25.442323082854756	17.69457085004075	21.623639417438486	35.23946664966601
10-11	24.134716857533096	27.040361225816817	28.761812339577915	20.063109577072176
12-13	25.032767160026054	24.426769449026406	29.045403401839188	21.495059989108356
14-15	22.11864335451193	25.9902093391286	26.8056500451192	25.08549726124027
16-17	21.65562310253565	29.07224749140282	27.53700374474154	21.735125661319994
18-19	21.581134783296207	28.248497474166367	29.261848424194635	20.908519318342787
20-21	22.427662572574263	27.37778677965334	29.197828124408186	20.99672252336421
22-23	22.06149010758754	27.268279360474228	29.06860440647764	21.601626125460594
24-25	21.813196663661973	27.313424388737715	29.236649068942043	21.636729878658265
26-27	21.793079112805973	27.536483169760423	29.12575179577933	21.54468592165427
28-29	21.680340961804845	27.64831030434034	29.139910695872338	21.531438037982475
30-31	22.04878359330447	27.44142131105795	29.03897953108356	21.47081556455402
32-33	21.8174134910438	27.542257543419996	29.043682942162423	21.596646023373772
34-35	21.925702251554718	27.631051759687068	29.030408123842488	21.412837864915723
36-37	21.86995844691445	27.533598905029695	29.032196252259308	21.564246395796552
38-39	21.73777125129123	27.69518747144801	29.071808022665113	21.49523325459565
40-41	22.053922516378	27.522121102839648	28.936399915350414	21.487556465431933
42-43	21.89419613636161	27.576815815222844	28.856696960642115	21.672291087773434
44-45	21.95617534973187	27.707824920001606	28.693797111888074	21.642202618378445
46-47	21.98770919749828	27.68746191696769	28.430648439861216	21.894180445672813
48-49	21.972053103980652	27.722884260402864	28.541774515547612	21.76328812006887
50-51	21.969759302634454	27.947624320067355	28.344245565995017	21.73837081130317
52-53	22.10422276693149	27.87666164627639	28.262586228022567	21.75652935876955
54-55	22.02546659555172	27.78985918855794	28.343862581279954	21.840811634610386
56-57	22.072963025343054	27.889454138795845	28.234888113038764	21.802694722822338
58-59	22.042413025046667	27.94622646561094	28.27439394056569	21.7369665687767
60-61	22.13751689246696	27.894005844707227	28.31030925240935	21.658168010416468
62-63	22.08486098278143	27.881252025636567	28.286274918126743	21.747612073455265
64-65	22.01544451082359	27.878354429872214	28.28150748369145	21.82469357561275
66-67	22.133951489063154	27.876744249524098	28.16993688705482	21.819367374357928
68-69	22.051976651453103	27.843770548359526	28.287763026652605	21.816489773534762
70-71	22.029526298339583	27.70839639951318	28.349035525050404	21.913041777096833
72-73	22.08860282714848	27.735605664271834	28.277209966350398	21.89858154222929
74-75	21.944497515789436	27.8472631028784	28.27225191248108	21.935987468851078
76-77	22.044636229608212	27.726180053174982	28.220588283505275	22.008595433711534
78-79	21.989097465955208	27.83283094324344	28.174036952791276	22.004034638010072
80-81	22.0892082967451	27.85151013803181	28.10962970694334	21.949651858279754
82-83	22.111953889706594	27.81832920752725	28.195989655501364	21.873727247264792
84-85	22.265282921322232	27.729760372375033	28.14596983509005	21.85898687121268
86-87	21.995355444275813	27.837819221699174	28.188684272143856	21.978141061881157
88-89	22.10218268004839	27.827268976440156	28.11168046434309	21.95886787916836
90-91	22.152160516606003	27.831663851118076	28.133748594678277	21.882427037597648
92-93	22.185563533628116	27.79591010752152	28.117338715720088	21.901187643130278
94-95	22.053979168329015	27.94973292581247	28.084860091056168	21.91142781480235
96-97	22.1606187925232	28.037257190633095	28.144139108542333	21.657984908301373
98-99	23.198489370842186	29.933474937905842	28.388512146204715	18.479523545047265
100	22.13322061682803	30.847593096989623	22.331216057063	24.687970229119347
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	659.0
1	659.0
2	929.5
3	1324.0
4	1758.0
5	2559.5
6	3268.0
7	4044.5
8	5263.5
9	6064.0
10	5891.5
11	5690.0
12	6296.0
13	7863.5
14	10922.0
15	14453.0
16	17608.5
17	20242.0
18	21138.0
19	20136.0
20	18128.5
21	16595.5
22	16992.0
23	19542.5
24	24456.5
25	32003.5
26	42352.5
27	54303.5
28	65758.0
29	77997.0
30	90038.0
31	102594.0
32	118061.5
33	133040.0
34	147444.0
35	161859.5
36	176810.5
37	190109.0
38	198968.5
39	206817.0
40	216290.0
41	224389.0
42	230298.0
43	235872.5
44	239732.5
45	242637.0
46	244070.0
47	244293.5
48	242631.0
49	236922.0
50	229735.5
51	220193.5
52	207754.5
53	194355.0
54	179607.5
55	164078.5
56	149378.0
57	133631.0
58	117093.0
59	101194.5
60	84019.0
61	67127.0
62	52215.0
63	40001.5
64	30081.5
65	21880.5
66	15936.5
67	12061.5
68	9047.0
69	6469.0
70	4563.0
71	3141.0
72	2104.0
73	1366.0
74	873.5
75	542.5
76	348.5
77	238.5
78	160.0
79	104.0
80	68.5
81	42.0
82	25.5
83	14.5
84	11.0
85	11.5
86	8.5
87	4.0
88	1.0
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005551479496147721
3	2.5071197724538095E-4
4	7.700439301108129E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	8.954434553873504E-6
24-25	3.581989684227909E-5
26-27	6.537798596039097E-4
28-29	0.001585668708297249
30-31	9.235495542169766E-4
32-33	8.983336091217513E-4
34-35	0.0012877954454907708
36-37	0.0
38-39	2.1810106130702698E-4
40-41	9.78122775799085E-4
42-43	4.322568054235537E-4
44-45	0.0
46-47	0.0
48-49	0.0018269427535021973
50-51	0.003729484277803875
52-53	0.0
54-55	0.003706440301039969
56-57	0.039455383733688384
58-59	0.056931639961921646
60-61	0.030531564903004328
62-63	0.006622814881286044
64-65	0.0
66-67	0.0
68-69	7.140597653742423E-5
70-71	0.003891932764699305
72-73	0.008093536765436215
74-75	0.005522841192640264
76-77	0.007169573851316362
78-79	0.010771967901884347
80-81	0.011331004960953314
82-83	0.042811494989814174
84-85	0.024920196038875506
86-87	0.0010204297806220162
88-89	0.0015011252277873516
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	44.0
20-21	152.0
22-23	333.0
24-25	454.0
26-27	1257.0
28-29	3188.0
30-31	10001.0
32-33	12680.0
34-35	17605.0
36-37	28175.0
38-39	32558.0
40-41	32480.0
42-43	36706.0
44-45	43598.0
46-47	44818.0
48-49	41568.0
50-51	38002.0
52-53	36990.0
54-55	40225.0
56-57	42294.0
58-59	42006.0
60-61	40987.0
62-63	39565.0
64-65	41738.0
66-67	43948.0
68-69	45122.0
70-71	44719.0
72-73	41454.0
74-75	42269.0
76-77	44357.0
78-79	45716.0
80-81	44946.0
82-83	42928.0
84-85	42447.0
86-87	44075.0
88-89	45726.0
90-91	46818.0
92-93	45958.0
94-95	53824.0
96-97	170512.0
98-99	380960.0
100-101	3730894.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.6875632562008
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.62853522527944	83.07627612346474
2	5.509509443544564	9.882689534570774
3	1.1308692142428678	3.0427471256069194
4	0.35819693854737356	1.2850324233658015
5	0.15142996711916193	0.6790692377442122
6	0.07416595367124132	0.3991058196807548
7	0.04493770393674547	0.28212472150906986
8	0.027585751688137133	0.19792790795997178
9	0.018442365695968955	0.1488645755956083
>10	0.054253915932548885	0.7495136974747783
>50	0.0012124723205921351	0.07402220904878717
>100	8.067900767831538E-4	0.1374190427508875
>500	2.712897326654174E-5	0.01610733056918208
>1k	2.712897126008245E-5	0.02910025065853301
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.790799837467007E-5
2	0.0	0.0	0.0	0.0	3.581599674934014E-5
3	0.0	0.0	0.0	0.0	3.581599674934014E-5
4	0.0	7.163199349868028E-5	0.0	0.0	3.581599674934014E-5
5	0.0	7.163199349868028E-5	0.0	0.0	3.581599674934014E-5
6	0.0	7.163199349868028E-5	0.0	0.0	3.581599674934014E-5
7	0.0	7.163199349868028E-5	0.0	0.0	3.581599674934014E-5
8	0.0	8.953999187335034E-5	0.0	0.0	3.581599674934014E-5
9	0.0	1.611719853720306E-4	0.0	0.0	3.581599674934014E-5
10-11	0.0	1.611719853720306E-4	0.0	1.790799837467007E-5	3.581599674934014E-5
12-13	0.0	1.611719853720306E-4	0.0	1.790799837467007E-5	5.3723995124010207E-5
14-15	0.0	2.59665976432716E-4	0.0	3.581599674934014E-5	7.163199349868028E-5
16-17	0.0	2.865279739947211E-4	0.0	5.37239951240102E-5	8.953999187335034E-5
18-19	0.0	3.0443597236939115E-4	0.0	5.37239951240102E-5	1.074479902480204E-4
20-21	0.0	3.223439707440612E-4	0.0	9.849399106068538E-5	1.074479902480204E-4
22-23	8.953999187335034E-6	3.671139666807364E-4	0.0	1.4326398699736055E-4	1.2535598862269048E-4
24-25	3.581599674934014E-5	4.1188396261741155E-4	0.0	1.4326398699736055E-4	1.4326398699736055E-4
26-27	3.581599674934014E-5	4.476999593667517E-4	0.0	1.5221798618469558E-4	1.4326398699736055E-4
28-29	3.581599674934014E-5	4.566539585540867E-4	0.0	3.492059683060663E-4	1.4326398699736055E-4
30-31	3.581599674934014E-5	5.551479496147721E-4	0.0	0.001343099878100255	1.4326398699736055E-4
32-33	3.581599674934014E-5	5.551479496147721E-4	0.0	0.003205531709065942	1.7907998374670067E-4
34-35	3.581599674934014E-5	6.536419406754574E-4	0.0	0.005730559479894422	2.148959804960408E-4
36-37	3.581599674934014E-5	7.521359317361428E-4	0.0	0.009607641128010491	2.148959804960408E-4
38-39	3.581599674934014E-5	7.521359317361428E-4	0.0	0.015714268573772984	2.2384997968337585E-4
40-41	3.581599674934014E-5	8.953999187335034E-4	0.0	0.021695540030912785	2.5071197724538095E-4
42-43	3.581599674934014E-5	9.222619162955085E-4	0.0	0.028187189441730688	2.5071197724538095E-4
44-45	3.581599674934014E-5	0.001056571904105534	0.0	0.03488478083385729	2.5071197724538095E-4
46-47	3.581599674934014E-5	0.0011282038976042142	0.0	0.042164382173160674	2.5071197724538095E-4
48-49	3.581599674934014E-5	0.0011282038976042142	0.0	0.04954247750352474	2.865279739947211E-4
50-51	3.581599674934014E-5	0.0012087898902902296	0.0	0.056589274863957414	2.865279739947211E-4
52-53	3.581599674934014E-5	0.0012177438894775646	0.0	0.06360025622764075	2.954819731820561E-4
54-55	3.581599674934014E-5	0.0012446058870395696	0.0	0.06998445764821062	3.0443597236939115E-4
56-57	3.581599674934014E-5	0.0012714678846015748	0.0	0.07591200511022642	3.0443597236939115E-4
58-59	3.581599674934014E-5	0.0013072838813509148	0.0	0.08140976061125013	3.0443597236939115E-4
60-61	3.581599674934014E-5	0.0013610078764749252	0.0	0.08697019410658519	3.0443597236939115E-4
62-63	3.581599674934014E-5	0.0013789158748495952	0.0	0.09273656958322896	3.402519691187313E-4
64-65	3.581599674934014E-5	0.0015400878602216258	0.0	0.09810001509644262	3.402519691187313E-4
66-67	3.581599674934014E-5	0.0015579958585962958	0.0	0.10362463259502835	3.5815996749340135E-4
68-69	3.581599674934014E-5	0.0015848578561583008	0.0	0.10858514814481196	3.5815996749340135E-4
70-71	3.581599674934014E-5	0.0016206738529076412	0.0	0.11281143576123409	3.671139666807364E-4
72-73	3.581599674934014E-5	0.0016296278520949762	0.0	0.11660793141666415	5.014239544907619E-4
74-75	3.581599674934014E-5	0.0016923058464063214	0.0	0.11948216515579868	5.014239544907619E-4
76-77	3.581599674934014E-5	0.0017728918390923368	0.0	0.12267874286567729	5.014239544907619E-4
78-79	3.581599674934014E-5	0.0017907998374670068	0.0	0.12477397867551371	5.014239544907619E-4
80-81	3.581599674934014E-5	0.0017907998374670068	0.0	0.12546343661293852	5.19331952865432E-4
82-83	3.581599674934014E-5	0.0018266158342163468	0.0	0.1259559065682419	5.551479496147721E-4
84-85	3.581599674934014E-5	0.0018266158342163468	0.0	0.12616184854955062	5.551479496147721E-4
86-87	3.581599674934014E-5	0.001844523832591017	0.0	0.12628720453817333	5.551479496147721E-4
88	3.581599674934014E-5	0.001880339829340357	0.0	0.126305112536548	5.551479496147721E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7230	0.0	46.658085	1
GTATCAA	12710	0.0	36.434982	1
TCAACGC	13970	0.0	32.28598	4
ATCAACG	14110	0.0	31.965635	3
CAACGCA	14465	0.0	31.150908	5
TATCAAC	14615	0.0	31.010427	2
AACGCAG	14885	0.0	30.243114	6
ACGCAGA	17460	0.0	25.758282	7
CGCAGAG	17615	0.0	25.531626	8
GCAGAGT	21210	0.0	21.224363	9
GTACATG	16395	0.0	21.020681	1
TACATGG	15985	0.0	20.727518	2
GTGGTAT	3895	0.0	20.6052	1
TGGTATC	3670	0.0	20.114304	2
GAGTACT	12385	0.0	20.079704	12-13
ACATGGG	16180	0.0	19.812979	3
CAGAGTA	20440	0.0	19.021605	10-11
GATCAAC	2245	0.0	18.161425	1
CATGGGG	11230	0.0	18.11366	4
GTACTTT	13705	0.0	18.004978	14-15
>>END_MODULE