FastQCFastQC Report
Fri 27 May 2016
SRR2140110_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140110_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7487874
Sequences flagged as poor quality0
Sequence length19-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGT1050.001923753336.67574794-95
GAGTTGC10900.031.01337694-95
CCCGGTT5853.6379788E-1223.03989294-95
GTTTACG2100.002033323722.92234294-95
CGGGGGC5750.022.12978290-91
GAGTACG49500.022.1151921
AGTACGC49750.021.7164252
GTCGGGG5650.021.32267688-89
TCGGGGG5850.020.94588590-91
GTACGCA50350.020.8889473
TACATGG102400.020.4026872
GTACATG107350.020.2283821
ACATGGG102800.019.8706323
GCTCGAG7000.018.85129790-91
GTACTAG8800.018.7003461
CCCGCAG7801.2732926E-1118.514294-95
GGTCGGG6600.018.25350288-89
CGCATCG3200.00108973418.05134494-95
ATCGATC3800.00881211617.79188594-95
TCGAGTT7150.017.65936392-93
Produced by FastQC (version 0.11.5)