##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140110_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7487874
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51907230276578	33.0	31.0	34.0	31.0	34.0
2	32.68008916816709	34.0	31.0	34.0	31.0	34.0
3	32.85878501694874	34.0	31.0	34.0	31.0	34.0
4	36.2036869210139	37.0	35.0	37.0	35.0	37.0
5	36.01083244723402	37.0	35.0	37.0	35.0	37.0
6	36.11340575442375	37.0	35.0	37.0	35.0	37.0
7	36.14538452436566	37.0	35.0	37.0	35.0	37.0
8	36.22353354236463	37.0	36.0	37.0	35.0	37.0
9	38.003799476326655	39.0	38.0	39.0	35.0	39.0
10-11	37.968323452023895	39.0	38.0	39.0	35.0	39.0
12-13	37.97565230130742	39.0	38.0	39.0	35.0	39.0
14-15	39.1968933371475	40.0	39.0	41.0	36.0	41.0
16-17	39.26121085905025	40.0	39.0	41.0	36.5	41.0
18-19	39.33550751521727	40.0	39.0	41.0	36.5	41.0
20-21	39.3596323459866	40.0	39.0	41.0	37.0	41.0
22-23	39.3564601709394	40.0	39.0	41.0	37.0	41.0
24-25	39.29598406998831	40.0	39.0	41.0	36.5	41.0
26-27	39.12886987990859	40.0	39.0	41.0	36.0	41.0
28-29	39.0493385179018	40.0	39.0	41.0	36.0	41.0
30-31	39.12687925617411	40.0	39.0	41.0	36.0	41.0
32-33	39.07168045904146	40.0	39.0	41.0	36.0	41.0
34-35	38.95946103751318	40.0	38.0	41.0	35.0	41.0
36-37	38.846865218317454	40.0	38.0	41.0	35.0	41.0
38-39	38.7484005918453	40.0	38.0	41.0	35.0	41.0
40-41	38.83784976201103	40.0	38.0	41.0	35.0	41.0
42-43	38.81880440599757	40.0	38.0	41.0	35.0	41.0
44-45	38.78510006937967	40.0	38.0	41.0	35.0	41.0
46-47	38.70777789685586	40.0	38.0	41.0	35.0	41.0
48-49	38.659450577370464	40.0	38.0	41.0	35.0	41.0
50-51	38.54856426508937	40.0	38.0	41.0	34.0	41.0
52-53	38.5003027895318	40.0	38.0	41.0	34.0	41.0
54-55	38.394363335569	40.0	38.0	41.0	34.0	41.0
56-57	38.21975927933378	40.0	37.5	41.0	34.0	41.0
58-59	37.997240276301156	39.5	37.0	41.0	33.5	41.0
60-61	37.66069402518955	39.0	36.0	41.0	33.0	41.0
62-63	37.561842362129866	39.0	36.0	41.0	33.0	41.0
64-65	37.254729373271104	39.0	36.0	40.0	32.5	41.0
66-67	36.85596073263024	38.0	35.0	39.5	32.0	41.0
68-69	36.490516430658225	37.0	35.0	39.0	32.0	41.0
70-71	35.81025032013258	36.5	35.0	39.0	31.0	40.0
72-73	35.42961437186514	36.0	34.0	37.5	31.0	39.5
74-75	35.10634938559547	35.5	34.0	37.0	31.0	39.0
76-77	33.36135703648919	34.5	32.0	35.5	29.0	37.5
78-79	34.360104167972764	35.0	34.0	36.0	31.0	37.5
80-81	34.31280175485185	35.0	34.0	36.0	31.0	37.0
82-83	34.0406448591389	35.0	34.0	35.5	31.0	37.0
84-85	33.897006938542674	35.0	34.0	35.0	31.0	36.0
86-87	33.907833851423455	35.0	34.0	35.0	31.0	36.0
88-89	33.76032536835485	35.0	34.0	35.0	31.0	36.0
90-91	33.7721399681627	35.0	34.0	35.0	31.0	36.0
92-93	33.72372968157538	35.0	34.0	35.0	31.0	35.5
94-95	33.61159067253381	35.0	34.0	35.0	31.0	35.0
96-97	33.71952815637148	35.0	34.0	35.0	31.0	35.0
98-99	33.79330593355989	35.0	34.0	35.0	31.0	35.0
100-101	32.62217505621924	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	12.0
19	36.0
20	59.0
21	176.0
22	468.0
23	1434.0
24	3488.0
25	7478.0
26	13696.0
27	22524.0
28	33996.0
29	48444.0
30	65934.0
31	87532.0
32	117996.0
33	164087.0
34	246611.0
35	402365.0
36	785950.0
37	1755416.0
38	2516501.0
39	1183456.0
40	30212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	54.15654697181069	15.871688011844217	10.67265554949242	19.299109466852673
2	12.3359464729293	25.174310211277014	33.11407890168007	29.375664414113622
3	31.047107819019732	24.48155971198118	29.61199512169324	14.859337347305846
4	11.240266596366338	15.945447265806024	46.26303807996769	26.55124805785995
5	10.941597040762172	41.357279783286955	34.35218060560314	13.348942570347738
6	24.597407370508083	36.07905989469329	17.40355899603651	21.919973738762117
7	21.27764169108615	34.416017149861226	26.695841837082195	17.61049932197043
8	21.150569574220935	24.05692189799134	19.88547082923671	34.90703769855102
9	19.244994804262735	23.99153884770153	29.247293188489277	27.516173159546458
10-11	26.562571782974224	21.621272226679817	23.89354057400738	27.92261541633858
12-13	33.31064769067859	21.475953908792768	25.902498835112723	19.310899565415916
14-15	26.93805344480957	22.902502098726554	32.07794361924359	18.081500837220286
16-17	17.766771983609768	29.82907431401757	31.149402353725502	21.25475134864716
18-19	17.994546916788398	33.561969659211684	24.891037162217206	23.552446261782716
20-21	22.227570326813915	26.861724870930637	22.555728806211455	28.35497599604399
22-23	21.855626091688904	23.643967532549755	27.415185516179623	27.085220859581717
24-25	28.268541059737718	23.88013007552829	24.256905479067086	23.594423385666904
26-27	23.175401107775766	22.97185372746814	35.147848380374924	18.704896784381177
28-29	19.554691049790573	32.20200805223332	26.406697541460616	21.836603356515493
30-31	19.339316774029438	26.57688526506115	26.29328942574094	27.790508535168467
32-33	24.313473619545768	24.64479872852845	26.486072608506127	24.555655043419655
34-35	27.933014478773405	24.237225102145654	25.08432849268915	22.74543192639179
36-37	23.82549844213732	22.349067485831682	33.594560428922776	20.23087364310822
38-39	22.33546798340932	28.67211481772201	28.79349796064253	20.198919238226143
40-41	20.20316128470177	30.21065615977149	28.74394496543105	20.842237590095696
42-43	19.874521743718105	27.840544851820454	21.97309360313597	30.311839801325473
44-45	22.273674317501598	24.821798455009464	24.813688029285018	28.09083919820392
46-47	29.370753438633514	24.995519923374385	25.975158169221736	19.658568468770373
48-49	23.53255384385531	25.52057804425133	29.430193581960268	21.516674529933088
50-51	18.28659141290398	28.224957544173506	32.560880358832755	20.927570684089762
52-53	20.358349718089237	29.755721052103855	25.58957730317874	24.29635192662817
54-55	20.420947020374374	25.048320009567593	27.088203734063317	27.442529235994716
56-57	25.227151559645854	23.574398909252995	23.462142863385687	27.73630666771546
58-59	27.739913339264294	24.273054649635004	27.094205721411456	20.892826289689246
60-61	25.730887912606203	24.900422550684723	30.509873679363313	18.85881585734576
62-63	19.565674481288138	30.019232457918232	29.32209330475676	21.09299975603687
64-65	19.82061972201719	30.431070671969213	24.62749544313607	25.12081416287753
66-67	21.82901052560831	27.397376571793487	23.552005411423753	27.221607491174453
68-69	23.366146369333123	24.540936696821042	27.09228055246793	25.000636381377905
70-71	26.30148277349631	24.1135931056689	25.537397129765576	24.047526991069212
72-73	22.02093133895538	25.21160093860566	32.52678404964268	20.24068367279628
74-75	21.02538924566209	29.604970311429273	26.160882167188166	23.20875827572047
76-77	19.697442213200407	26.708107039239863	26.751110279282464	26.843340468277262
78-79	23.928975878335088	24.25432376534768	26.56167454111511	25.25502581520212
80-81	26.013603475661096	24.955678593726596	25.17106106793292	23.859656862679383
82-83	22.621051127993397	24.118116717560206	31.744911097783802	21.515921056662595
84-85	22.591438873439664	26.826980584365383	28.050572682317465	22.531007859877494
86-87	19.97946603628724	29.07048005614786	29.108638796787044	21.841415110777856
88-89	21.047565716798992	25.323972768825563	24.326899340920527	29.30156217345492
90-91	20.571428658668122	25.73346489348498	26.24884562989715	27.446260817949746
92-93	25.568405783831043	24.24181854117836	27.99757908994775	22.19219658504284
94-95	21.942567632355207	22.831119596486282	31.393462729560795	23.83285004159772
96-97	19.35165923514984	26.059581930444953	32.14564855944261	22.443110274962596
98-99	20.22394802044595	25.68157414493993	29.926653049910552	24.16782478470357
100-101	31.071307428962808	15.314414215800731	39.57423116423418	14.040047191002285
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	711.0
1	748.5
2	1278.0
3	1921.5
4	2967.0
5	5011.0
6	6439.5
7	7097.5
8	7167.0
9	6436.5
10	5273.0
11	4296.5
12	4536.0
13	6015.5
14	9377.0
15	13699.5
16	16710.0
17	18064.5
18	17923.0
19	16633.5
20	14883.5
21	13203.0
22	12110.0
23	12253.0
24	13868.0
25	16340.0
26	19592.5
27	23811.5
28	28564.5
29	34124.0
30	39110.0
31	43574.5
32	49470.5
33	56011.0
34	63003.5
35	71161.5
36	79707.0
37	89101.5
38	99783.5
39	116407.0
40	142378.0
41	182517.0
42	252140.0
43	384398.5
44	584485.5
45	860614.0
46	1187784.5
47	1414968.5
48	1377205.5
49	1064970.5
50	697546.0
51	434293.0
52	280539.5
53	205585.5
54	158365.0
55	127332.5
56	110594.5
57	96430.5
58	82324.5
59	70214.0
60	56765.0
61	45320.0
62	36324.0
63	27926.5
64	20423.0
65	14819.5
66	10951.0
67	8646.5
68	6973.5
69	5199.5
70	3803.0
71	2730.5
72	2002.0
73	1437.0
74	973.5
75	617.5
76	381.5
77	227.5
78	139.5
79	106.5
80	82.5
81	55.5
82	37.0
83	26.5
84	22.0
85	19.5
86	15.5
87	12.5
88	8.0
89	4.0
90	2.5
91	2.0
92	1.5
93	0.5
94	0.0
95	0.5
96	1.0
97	1.0
98	0.5
99	0.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	9.882644926984614E-4
3	4.0064776731018714E-4
4	0.0
5	0.0
6	4.273576184641996E-4
7	0.0
8	0.0
9	6.677462788503118E-5
10-11	3.4722806500216217E-4
12-13	3.3387313942515593E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.041844679049576E-5
28-29	0.0
30-31	2.0624647705236387E-5
32-33	1.803541850980551E-4
34-35	2.8025413444911848E-5
36-37	6.154027237866026E-4
38-39	1.9393683075183063E-4
40-41	2.9211236014299194E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	7.55956641045116E-4
56-57	1.1361935052906851E-4
58-59	0.0
60-61	7.683844908176681E-4
62-63	0.0
64-65	0.0
66-67	1.965217420355388E-5
68-69	0.0
70-71	0.0
72-73	1.0605223114804934E-4
74-75	0.0
76-77	1.1254445224502547E-5
78-79	8.196038948045426E-5
80-81	0.0
82-83	0.0
84-85	2.6522272343423114E-5
86-87	4.3109178583805076E-4
88-89	2.1845476748402732E-4
90-91	0.0
92-93	1.595222500039083E-5
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	4745.0
20-21	17868.0
22-23	14815.0
24-25	21505.0
26-27	96893.0
28-29	49675.0
30-31	56887.0
32-33	74034.0
34-35	58417.0
36-37	99233.0
38-39	122002.0
40-41	118657.0
42-43	137558.0
44-45	147496.0
46-47	119911.0
48-49	120815.0
50-51	169810.0
52-53	121976.0
54-55	172020.0
56-57	146205.0
58-59	121034.0
60-61	127869.0
62-63	107557.0
64-65	136900.0
66-67	134283.0
68-69	133554.0
70-71	114840.0
72-73	134902.0
74-75	133614.0
76-77	157973.0
78-79	176757.0
80-81	133184.0
82-83	174400.0
84-85	201712.0
86-87	158199.0
88-89	158635.0
90-91	145738.0
92-93	127223.0
94-95	176423.0
96-97	119586.0
98-99	772787.0
100-101	1970182.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.3431962193684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.25299489522173	76.17135856509194
2	7.563468836751837	12.9098121006798
3	1.854718249420451	4.748627504757989
4	0.6805108870033468	2.3230789663577225
5	0.2858109361020028	1.2196009400697292
6	0.14870581277662837	0.7614617615253871
7	0.07535080409544596	0.4501474921443382
8	0.04721783439270313	0.3223776724504083
9	0.027797697963760772	0.21351099525911751
>10	0.06227261786786392	0.7408632951008614
>50	6.042513904752361E-4	0.03595177122615901
>100	5.471770137927583E-4	0.10320893533659402
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	4.006477673101871E-5	2.6709851154012473E-5	0.0	0.0	0.0
3	4.006477673101871E-5	2.6709851154012473E-5	0.0	0.0	0.0
4	4.006477673101871E-5	2.6709851154012473E-5	0.0	0.0	0.0
5	9.348447903904366E-5	2.6709851154012473E-5	0.0	0.0	0.0
6	1.7361403250108109E-4	2.6709851154012473E-5	0.0	0.0	0.0
7	2.537435859631185E-4	2.6709851154012473E-5	0.0	0.0	0.0
8	3.0716328827114345E-4	2.6709851154012473E-5	0.0	1.3354925577006236E-5	0.0
9	3.205182138481497E-4	2.6709851154012473E-5	0.0	4.006477673101871E-5	0.0
10-11	3.4722806500216217E-4	2.6709851154012473E-5	0.0	6.677462788503118E-5	0.0
12-13	4.273576184641996E-4	2.6709851154012473E-5	0.0	1.3354925577006236E-4	0.0
14-15	5.07487171926237E-4	2.6709851154012473E-5	0.0	2.537435859631185E-4	0.0
16-17	5.141646347147401E-4	4.006477673101871E-5	0.0	4.807773207722245E-4	0.0
18-19	6.410364276962994E-4	4.674223951952183E-5	0.0	6.877786672158212E-4	0.0
20-21	7.478758323123493E-4	5.3419702308024946E-5	0.0	0.001128491211257027	0.0
22-23	8.012955346203743E-4	5.3419702308024946E-5	0.0	0.0018429797296268607	0.0
24-25	0.001014974343852474	5.3419702308024946E-5	0.0	0.002537435859631185	0.0
26-27	0.001014974343852474	5.3419702308024946E-5	0.0	0.0031717948245389813	0.0
28-29	0.0012286531530845738	5.3419702308024946E-5	0.0	0.0044338352915660705	0.0
30-31	0.0013755573344316424	5.3419702308024946E-5	0.0	0.006704172639657131	0.0
32-33	0.0014222995739511643	5.3419702308024946E-5	0.0	0.011912593614689562	0.0
34-35	0.0016226234576062577	6.677462788503118E-5	0.0	0.01732801593616559	0.0
36-37	0.0018162698784728482	8.012955346203742E-5	0.0	0.022442952432158984	0.0
38-39	0.0019431416714544075	8.680701625054054E-5	0.0	0.029928388218070978	0.0
40-41	0.0019631740598199166	9.348447903904366E-5	0.0	0.03762082535042657	0.0
42-43	0.0020900458528014757	1.2019433019305613E-4	0.0	0.047029370419427466	0.0
44-45	0.0022970471992450727	1.469041813470686E-4	0.0	0.056250946530350274	0.0
46-47	0.002704372429343763	1.469041813470686E-4	0.0	0.06618033369685442	0.0
48-49	0.0027911794455943036	1.469041813470686E-4	0.0	0.07434687068719373	0.0
50-51	0.0027911794455943036	1.469041813470686E-4	0.0	0.0826603118588801	0.0
52-53	0.002797856908382807	1.6025910692407484E-4	0.0	0.09011903779363808	0.0
54-55	0.002817889296748316	1.6025910692407484E-4	0.0	0.0989065788233082	0.0
56-57	0.0030048582548264035	1.6025910692407484E-4	0.0	0.10896283778279389	0.0
58-59	0.0030182131804034097	1.6025910692407484E-4	0.0	0.11675543685697704	0.0
60-61	0.0030248906431919126	1.7361403250108109E-4	0.0	0.12367328830586626	0.0
62-63	0.003044923031557422	1.7361403250108109E-4	0.0	0.13026394407811884	0.0
64-65	0.003098342733865447	1.7361403250108109E-4	0.0	0.13624027327382915	0.0
66-67	0.003392151096559584	1.7361403250108109E-4	0.0	0.14368564428301012	0.0
68-69	0.0034589257244446153	1.8696895807808733E-4	0.0	0.15104420827594053	0.0
70-71	0.003565765129060665	2.070013464435967E-4	0.0	0.1573877979250185	0.0
72-73	0.003659249608099709	2.2703373480910602E-4	0.0	0.161641341721295	0.0
74-75	0.0037059918476192305	2.2703373480910602E-4	0.0	0.16538072088285674	0.0
76-77	0.003712669310407734	2.3371119759760915E-4	0.0	0.16894648601191742	0.0
78-79	0.003712669310407734	2.4038866038611227E-4	0.0	0.1732400945849249	0.0
80-81	0.003966412896370852	2.4038866038611227E-4	0.0	0.17498958983551272	0.0
82-83	0.004033187524255883	2.4038866038611227E-4	0.0	0.17553714178416996	0.0
84-85	0.004193446631179958	2.4038866038611227E-4	0.0	0.1759912092537882	0.0
86-87	0.00434702827531553	2.4038866038611227E-4	0.0	0.17610472612119274	0.0
88-89	0.00436706066368104	2.4038866038611227E-4	0.0	0.17615146836071227	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTCGT	105	0.0019237533	36.675747	94-95
GAGTTGC	1090	0.0	31.013376	94-95
CCCGGTT	585	3.6379788E-12	23.039892	94-95
GTTTACG	210	0.0020333237	22.922342	94-95
CGGGGGC	575	0.0	22.129782	90-91
GAGTACG	4950	0.0	22.115192	1
AGTACGC	4975	0.0	21.716425	2
GTCGGGG	565	0.0	21.322676	88-89
TCGGGGG	585	0.0	20.945885	90-91
GTACGCA	5035	0.0	20.888947	3
TACATGG	10240	0.0	20.402687	2
GTACATG	10735	0.0	20.228382	1
ACATGGG	10280	0.0	19.870632	3
GCTCGAG	700	0.0	18.851297	90-91
GTACTAG	880	0.0	18.700346	1
CCCGCAG	780	1.2732926E-11	18.5142	94-95
GGTCGGG	660	0.0	18.253502	88-89
CGCATCG	320	0.001089734	18.051344	94-95
ATCGATC	380	0.008812116	17.791885	94-95
TCGAGTT	715	0.0	17.659363	92-93
>>END_MODULE