FastQCFastQC Report
Fri 27 May 2016
SRR2140109_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140109_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3948187
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42450.043.7086451
GTATCAA76350.033.31421
ATCAACG90150.027.2142853
CAACGCA90050.027.1990225
TCAACGC91150.026.9606554
TATCAAC94600.026.4976372
AACGCAG94250.026.2042546
ACGCAGA104100.023.6854487
GTACATG120300.023.4244631
TACATGG118800.023.3754562
ACATGGG118300.022.8504643
CGCAGAG112050.021.9684018
TAACGCC2659.094947E-1221.6380424
CATGGGG85750.020.3952774
TATACCG2553.1159288E-919.2742235
GTACTAG10400.018.9040431
GCAGAGT134050.018.3629959
GAGTACT69250.017.97999812-13
AGAGTAC119250.016.84676410-11
CAGAGTA129350.016.44958510-11
Produced by FastQC (version 0.11.5)