##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140109_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3948187
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.814449518221906	34.0	31.0	34.0	31.0	34.0
2	33.00648905434317	34.0	33.0	34.0	31.0	34.0
3	33.1124009577054	34.0	33.0	34.0	31.0	34.0
4	36.323852441639666	37.0	37.0	37.0	35.0	37.0
5	36.194689866513414	37.0	35.0	37.0	35.0	37.0
6	36.4004078327597	37.0	37.0	37.0	35.0	37.0
7	36.40745562456895	37.0	37.0	37.0	35.0	37.0
8	36.40965840777045	37.0	37.0	37.0	35.0	37.0
9	38.22463956241181	39.0	38.0	39.0	37.0	39.0
10-11	38.13749513384244	39.0	38.0	39.0	37.0	39.0
12-13	38.11631161340635	39.0	38.0	39.0	37.0	39.0
14-15	39.56108309966068	41.0	39.0	41.0	37.0	41.0
16-17	39.53593902720414	41.0	39.0	41.0	37.0	41.0
18-19	39.575319887330565	41.0	39.0	41.0	37.0	41.0
20-21	39.59050769853229	41.0	39.0	41.0	37.0	41.0
22-23	39.54185848079568	41.0	39.0	41.0	37.0	41.0
24-25	39.5226726327753	41.0	39.0	41.0	37.0	41.0
26-27	39.407943594857855	41.0	39.0	41.0	37.0	41.0
28-29	39.34465838176353	40.0	39.0	41.0	37.0	41.0
30-31	39.290444349194374	40.0	39.0	41.0	36.5	41.0
32-33	39.20748548745873	40.0	39.0	41.0	36.0	41.0
34-35	39.17198582328993	40.0	38.0	41.0	36.0	41.0
36-37	39.13409522685304	40.0	38.0	41.0	36.0	41.0
38-39	39.07898987149186	40.0	38.0	41.0	35.0	41.0
40-41	39.01270840324924	40.0	38.0	41.0	35.0	41.0
42-43	39.00389675432265	40.0	38.0	41.0	35.0	41.0
44-45	38.94225634936323	40.0	38.0	41.0	35.0	41.0
46-47	38.83034214108748	40.0	38.0	41.0	35.0	41.0
48-49	38.815249442526266	40.0	38.0	41.0	35.0	41.0
50-51	38.71204194487386	40.0	38.0	41.0	35.0	41.0
52-53	38.54890692179151	40.0	38.0	41.0	35.0	41.0
54-55	38.3504329634186	40.0	37.5	41.0	34.5	41.0
56-57	38.200897633583025	40.0	37.0	41.0	34.0	41.0
58-59	38.0354720921853	39.0	37.0	41.0	34.0	41.0
60-61	37.80257007531447	39.0	36.0	41.0	34.0	41.0
62-63	37.63720433578598	39.0	36.0	41.0	34.0	41.0
64-65	37.35908045060364	38.5	35.0	40.0	33.5	41.0
66-67	37.115258384438135	38.0	35.0	40.0	33.0	41.0
68-69	36.84668120235507	37.5	35.0	40.0	33.0	41.0
70-71	36.42164088603646	37.0	35.0	39.0	33.0	41.0
72-73	36.161628524056475	36.5	35.0	39.0	33.0	41.0
74-75	35.82326560587906	36.0	35.0	38.5	32.5	40.0
76-77	34.145403400140076	34.5	33.0	36.5	30.0	39.0
78-79	35.09934392159043	35.0	34.0	37.0	32.0	39.0
80-81	35.036735478010684	35.0	34.0	37.0	32.0	39.0
82-83	34.80027206467413	35.0	34.0	36.5	32.0	38.0
84-85	34.58635217546733	35.0	34.0	36.0	32.0	37.5
86-87	34.42423627766824	35.0	34.0	36.0	32.0	37.0
88-89	34.28219748198821	35.0	34.0	35.5	31.5	37.0
90-91	34.227976028298016	35.0	34.0	35.0	32.0	36.5
92-93	34.12427928365884	35.0	34.0	35.0	31.5	36.0
94-95	33.98768925358297	35.0	34.0	35.0	31.0	36.0
96-97	34.013652468163016	35.0	34.0	35.0	31.0	36.0
98-99	34.029210381905926	35.0	34.0	35.0	31.0	36.0
100-101	32.72032876818463	34.0	31.5	35.0	28.5	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	2.0
18	6.0
19	15.0
20	47.0
21	105.0
22	271.0
23	777.0
24	1773.0
25	3579.0
26	6216.0
27	9910.0
28	14955.0
29	21298.0
30	29439.0
31	39777.0
32	54620.0
33	78193.0
34	125200.0
35	212251.0
36	447406.0
37	1069646.0
38	1209499.0
39	608281.0
40	14919.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.1401298874648	19.62006865429626	12.676780507103642	24.563020951135293
2	15.484337501978787	20.90853253126484	40.039943010922904	23.567186955833467
3	18.50643602461598	26.130910875857076	29.805198209700855	25.557454889826086
4	12.57726647699311	17.288137567952074	38.63656913920238	31.49802681585244
5	13.271610488560952	37.910767651076306	35.5158202992918	13.301801561070942
6	30.640120967741936	37.54141169557465	18.584302561193063	13.234164775490356
7	27.11533166995383	32.030372421569695	23.307229368821687	17.547066539654782
8	25.361792640520825	35.6183736991181	21.141247868958587	17.878585791402486
9	26.23673915989246	15.902015736344675	20.87789705902839	36.983348044734484
10-11	24.769140055412972	26.155604123730143	28.80867935624908	20.26657646460781
12-13	25.495723586230373	23.649462231597035	28.777980065691228	22.076834116481365
14-15	22.678155821900027	24.89966913927836	26.150711706411066	26.271463332410548
16-17	22.40416930606377	27.937493842110317	26.839825469259686	22.818511382566225
18-19	22.375700543059384	27.344512810563433	28.32535540996412	21.95443123641307
20-21	23.238873410887557	26.687552786781104	27.962515417317775	22.111058385013564
22-23	23.072404464844688	26.587308874911674	27.799333523229354	22.540953137014284
24-25	22.716713457224547	26.771562977751696	27.890128178218504	22.62159538680525
26-27	22.706543436353854	26.92055739998941	27.840996202079673	22.531902961577067
28-29	22.57739080683644	27.09554452532827	27.78828557400464	22.538779093830648
30-31	22.877558584535464	27.005333970002752	27.55660870865678	22.560498736805005
32-33	22.672641083371776	27.10608516833457	27.59519782158711	22.626075926706545
34-35	22.738438560642127	27.2044005676136	27.651879022805193	22.40528184893908
36-37	22.798829131122833	26.982528449026518	27.59717276242953	22.621469657421127
38-39	22.65041427926231	27.109067523045	27.683007161692746	22.55751103599994
40-41	22.83538171467142	26.944367057594793	27.738336413980015	22.48191481375377
42-43	22.749852512078064	27.00813442394672	27.450930369756204	22.791082694219007
44-45	22.770261094745166	27.323883063328818	27.21336250072269	22.692493341203328
46-47	22.8920846797034	27.198633449147962	27.034929205513087	22.87435266563555
48-49	22.914703601707966	27.116871184540674	26.995544446829356	22.972880766922
50-51	22.68769887319471	27.236133873617902	26.99578978145895	23.08037747172844
52-53	22.863426495405406	27.262737653026935	26.792477881993705	23.081357969573958
54-55	22.959489361117143	27.142082227592528	26.82752963805402	23.07089877323631
56-57	22.94603488142138	27.139913089319194	26.95411294676851	22.959939082490912
58-59	22.915765029035732	27.18868664629571	27.008420310310694	22.887128014357867
60-61	23.037126364244152	27.34186133766071	26.68428978076584	22.936722517329297
62-63	23.01018684776618	27.24209635666593	26.68630493167336	23.06141186389453
64-65	22.863450992150977	27.241671881503454	26.75613155755876	23.13874556878681
66-67	22.9234457002882	27.177579533625142	26.66426755547044	23.23470721061622
68-69	22.963496190624483	27.223032931126085	26.742058682457625	23.071412195791808
70-71	22.969322805926005	26.972433373368194	26.743995127919867	23.314248692785934
72-73	23.032759919771625	27.124847610144382	26.675934671180396	23.166457798903597
74-75	22.804523927900775	27.23932362884171	26.83190369399177	23.124248749265746
76-77	22.99605454510959	27.093239933645748	26.670367678780742	23.24033784246392
78-79	22.966198041526827	27.150642022738136	26.57761669003357	23.305543245701475
80-81	22.888850575940246	27.24589742064007	26.64735083760413	23.21790116581556
82-83	23.237881845923827	27.04818720918777	26.81602743209417	22.897903512794233
84-85	23.24889179519856	26.99681159708163	26.616517143306677	23.13777946441313
86-87	22.877032006166544	27.221302479420572	26.83086062320595	23.070804891206937
88-89	23.187394462918096	26.95870556698594	26.700098307288165	23.153801662807794
90-91	23.158593786349044	27.05455700693249	26.688176819196947	23.098672387521518
92-93	23.246372006271717	27.049647765179497	26.653059363276416	23.050920865272374
94-95	23.000123598799423	27.072319551848384	26.762778643552878	23.164778205799315
96-97	22.980713939639084	27.080636995540146	26.803989634604335	23.134659430216438
98-99	23.38389089413135	27.657572588882346	26.829587397254933	22.128949119731367
100-101	27.951870111741854	34.87078047800166	27.15015661805874	10.027192792197742
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	502.0
1	527.5
2	969.5
3	1480.0
4	2146.0
5	3664.5
6	4799.0
7	5284.5
8	5352.5
9	4916.5
10	4253.5
11	3648.0
12	3990.5
13	5526.5
14	8849.5
15	13093.5
16	16248.5
17	17944.0
18	18071.5
19	17036.5
20	15584.5
21	14441.0
22	14478.0
23	16250.5
24	20038.5
25	25362.5
26	32062.5
27	39970.0
28	47484.0
29	55043.0
30	62394.0
31	68722.0
32	76463.5
33	84701.0
34	91616.0
35	99908.0
36	110634.0
37	119378.0
38	123874.0
39	127510.0
40	132786.5
41	137742.0
42	141670.0
43	147860.5
44	155288.5
45	163337.0
46	169112.5
47	172852.0
48	177727.5
49	181411.0
50	183864.5
51	183328.5
52	178221.0
53	173024.0
54	166906.5
55	157524.5
56	147316.5
57	135959.0
58	123317.5
59	107569.0
60	88689.5
61	70355.0
62	55102.0
63	42691.0
64	32616.0
65	24557.5
66	18244.0
67	13765.5
68	10345.0
69	7597.0
70	5496.5
71	3932.0
72	2780.0
73	1948.5
74	1363.0
75	931.0
76	627.5
77	408.5
78	273.0
79	184.5
80	130.5
81	86.5
82	57.0
83	33.0
84	16.5
85	13.0
86	11.5
87	8.5
88	4.5
89	3.0
90	3.0
91	2.5
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0015703410198149176
3	5.065616192951347E-4
4	0.0
5	0.0
6	6.838581860484318E-4
7	0.0
8	0.0
9	5.065616192951347E-5
10-11	3.545931335065943E-4
12-13	4.8123353833037796E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	8.869493137609806E-5
28-29	0.0
30-31	3.811883062609163E-5
32-33	2.677616844505052E-4
34-35	2.5609703004274262E-5
36-37	7.73082902575835E-4
38-39	2.6015962353861835E-4
40-41	3.9442078650134304E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	7.446331994139164E-4
56-57	5.799454851243983E-5
58-59	0.0
60-61	8.330965454759138E-4
62-63	0.0
64-65	0.0
66-67	3.0889834186459074E-5
68-69	0.0
70-71	0.0
72-73	8.039553315986121E-5
74-75	0.0
76-77	1.6497822452414504E-5
78-79	3.343301386416936E-5
80-81	0.0
82-83	0.0
84-85	1.7423915857819453E-5
86-87	4.2381568679596935E-4
88-89	1.0743954376872133E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	58.0
20-21	247.0
22-23	515.0
24-25	911.0
26-27	2172.0
28-29	5996.0
30-31	13307.0
32-33	15521.0
34-35	21131.0
36-37	34001.0
38-39	41473.0
40-41	39724.0
42-43	42379.0
44-45	49465.0
46-47	49452.0
48-49	46861.0
50-51	42731.0
52-53	40129.0
54-55	42676.0
56-57	43810.0
58-59	43410.0
60-61	43218.0
62-63	40738.0
64-65	40348.0
66-67	42248.0
68-69	42844.0
70-71	43198.0
72-73	39596.0
74-75	39642.0
76-77	39262.0
78-79	41261.0
80-81	41944.0
82-83	38729.0
84-85	38240.0
86-87	38627.0
88-89	40704.0
90-91	42740.0
92-93	42363.0
94-95	44806.0
96-97	70490.0
98-99	330520.0
100-101	2230700.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.2661615920207
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.4410739483272	75.36855990293611
2	7.152073057126583	12.053554878995323
3	1.8939220744188208	4.787806306970142
4	0.683651885575053	2.3043488105022822
5	0.3220512874221144	1.35690129134151
6	0.16837292315772598	0.851288397031789
7	0.09988872204719382	0.5892067435274493
8	0.0606926064686381	0.4091466387301743
9	0.03913990748877902	0.29683527922315744
>10	0.13665220808965198	1.7182601393659105
>50	0.0014554436765107342	0.08458180318786995
>100	0.0010259362017346508	0.17950980818831477
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.5328080964756735E-5	0.0
2	0.0	0.0	0.0	2.5328080964756735E-5	0.0
3	0.0	0.0	0.0	2.5328080964756735E-5	0.0
4	0.0	0.0	0.0	2.5328080964756735E-5	0.0
5	0.0	0.0	0.0	5.065616192951347E-5	0.0
6	0.0	0.0	0.0	5.065616192951347E-5	0.0
7	0.0	0.0	0.0	5.065616192951347E-5	0.0
8	0.0	0.0	0.0	5.065616192951347E-5	0.0
9	0.0	0.0	0.0	5.065616192951347E-5	0.0
10-11	0.0	0.0	0.0	7.59842428942702E-5	0.0
12-13	0.0	0.0	0.0	8.864828337664857E-5	0.0
14-15	0.0	0.0	0.0	1.0131232385902694E-4	0.0
16-17	0.0	0.0	0.0	1.0131232385902694E-4	0.0
18-19	0.0	0.0	0.0	1.0131232385902694E-4	0.0
20-21	0.0	0.0	0.0	1.3930444530616204E-4	0.0
22-23	0.0	0.0	0.0	1.899606072356755E-4	0.0
24-25	0.0	0.0	0.0	2.5328080964756735E-4	0.0
26-27	0.0	0.0	0.0	4.305773764008645E-4	0.0
28-29	0.0	0.0	0.0	0.0012917321292025936	0.0
30-31	0.0	0.0	0.0	0.00419179739966724	0.0
32-33	0.0	0.0	0.0	0.009194093390206695	0.0
34-35	0.0	0.0	0.0	0.015171520497889284	2.5328080964756735E-5
36-37	0.0	0.0	0.0	0.023023225596963872	2.5328080964756735E-5
38-39	0.0	0.0	0.0	0.0350667280957057	2.5328080964756735E-5
40-41	0.0	0.0	0.0	0.047338183323130334	2.5328080964756735E-5
42-43	0.0	0.0	0.0	0.05811528177363433	2.5328080964756735E-5
44-45	0.0	0.0	0.0	0.06995615962465809	2.5328080964756735E-5
46-47	0.0	0.0	0.0	0.08210097444725895	2.5328080964756735E-5
48-49	0.0	0.0	0.0	0.09313135370741052	2.5328080964756735E-5
50-51	0.0	0.0	0.0	0.10503555176084617	2.5328080964756735E-5
52-53	0.0	0.0	0.0	0.11582531425183254	2.5328080964756735E-5
54-55	0.0	0.0	0.0	0.12607052300207666	2.5328080964756735E-5
56-57	0.0	0.0	0.0	0.135821834173508	2.5328080964756735E-5
58-59	0.0	0.0	0.0	0.1451805600899856	2.5328080964756735E-5
60-61	0.0	0.0	0.0	0.15474191065418127	2.5328080964756735E-5
62-63	0.0	0.0	0.0	0.16420194889451792	2.5328080964756735E-5
64-65	0.0	0.0	0.0	0.17296546490832373	2.5328080964756735E-5
66-67	0.0	0.0	0.0	0.1820582459746714	2.5328080964756735E-5
68-69	0.0	0.0	0.0	0.19159426845790234	2.5328080964756735E-5
70-71	0.0	0.0	0.0	0.1995599499213183	2.5328080964756735E-5
72-73	0.0	0.0	0.0	0.20482819076198772	2.5328080964756735E-5
74-75	0.0	0.0	0.0	0.20912130048551397	2.5328080964756735E-5
76-77	0.0	0.0	0.0	0.2132244496018046	2.5328080964756735E-5
78-79	0.0	0.0	0.0	0.21658042032963484	2.5328080964756735E-5
80-81	0.0	0.0	0.0	0.21798612882317883	2.5328080964756735E-5
82-83	0.0	0.0	0.0	0.2184800264019916	2.5328080964756735E-5
84-85	0.0	0.0	0.0	0.21892326781887483	2.5328080964756735E-5
86-87	0.0	0.0	0.0	0.21907523630466338	2.5328080964756735E-5
88-89	0.0	0.0	0.0	0.21911322842611053	2.5328080964756735E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4245	0.0	43.708645	1
GTATCAA	7635	0.0	33.3142	1
ATCAACG	9015	0.0	27.214285	3
CAACGCA	9005	0.0	27.199022	5
TCAACGC	9115	0.0	26.960655	4
TATCAAC	9460	0.0	26.497637	2
AACGCAG	9425	0.0	26.204254	6
ACGCAGA	10410	0.0	23.685448	7
GTACATG	12030	0.0	23.424463	1
TACATGG	11880	0.0	23.375456	2
ACATGGG	11830	0.0	22.850464	3
CGCAGAG	11205	0.0	21.968401	8
TAACGCC	265	9.094947E-12	21.638042	4
CATGGGG	8575	0.0	20.395277	4
TATACCG	255	3.1159288E-9	19.274223	5
GTACTAG	1040	0.0	18.904043	1
GCAGAGT	13405	0.0	18.362995	9
GAGTACT	6925	0.0	17.979998	12-13
AGAGTAC	11925	0.0	16.846764	10-11
CAGAGTA	12935	0.0	16.449585	10-11
>>END_MODULE