FastQCFastQC Report
Fri 27 May 2016
SRR2140108_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140108_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7081441
Sequences flagged as poor quality0
Sequence length19-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91500.044.7979351
GTATCAA160900.034.1203651
ATCAACG198100.026.8842353
CAACGCA200600.026.5285475
TCAACGC202250.026.4541284
TATCAAC207100.026.1323412
AACGCAG213450.025.123896
GTACATG251250.024.3492261
TACATGG252800.023.6643222
ACATGGG254850.023.0711383
ACGCAGA233500.022.8084247
GTACTAG18450.022.016711
CGCAGAG245950.021.653868
CATGGGG187400.021.5879674
GCAGAGT289450.018.3854359
GAGTACT148600.017.93342412-13
AGAGTAC257550.017.10198210-11
CAGAGTA274250.016.99564610-11
CTAGTAC22100.016.8949383
AGTACTT159750.016.51478612-13
Produced by FastQC (version 0.11.5)