##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140108_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7081441
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79618103151604	34.0	31.0	34.0	31.0	34.0
2	32.98528604559439	34.0	33.0	34.0	31.0	34.0
3	33.0915742996376	34.0	33.0	34.0	31.0	34.0
4	36.46119102030222	37.0	37.0	37.0	35.0	37.0
5	36.33197424083601	37.0	37.0	37.0	35.0	37.0
6	36.363734725742965	37.0	37.0	37.0	35.0	37.0
7	36.365461069293666	37.0	37.0	37.0	35.0	37.0
8	36.37728140360133	37.0	37.0	37.0	35.0	37.0
9	38.27697385320304	39.0	39.0	39.0	37.0	39.0
10-11	38.18638261619351	39.0	38.5	39.0	37.0	39.0
12-13	38.167816620939156	39.0	39.0	39.0	37.0	39.0
14-15	39.67303547399463	41.0	40.0	41.0	37.5	41.0
16-17	39.65105852043391	41.0	40.0	41.0	37.0	41.0
18-19	39.6882611886479	41.0	40.0	41.0	37.5	41.0
20-21	39.7039205674259	41.0	40.0	41.0	37.5	41.0
22-23	39.65320527971007	41.0	40.0	41.0	37.0	41.0
24-25	39.62615276409974	41.0	40.0	41.0	37.0	41.0
26-27	39.50453885380318	41.0	39.0	41.0	37.0	41.0
28-29	39.44125417303705	41.0	39.0	41.0	37.0	41.0
30-31	39.36895427086077	40.0	39.0	41.0	36.0	41.0
32-33	39.27855292028801	40.0	39.0	41.0	36.0	41.0
34-35	39.2414185550733	40.0	39.0	41.0	35.5	41.0
36-37	39.199486440218934	40.0	39.0	41.0	35.0	41.0
38-39	39.13381128934557	40.0	39.0	41.0	35.0	41.0
40-41	39.05958666659933	40.0	39.0	41.0	35.0	41.0
42-43	39.04354018782104	40.0	39.0	41.0	35.0	41.0
44-45	38.97606878175124	40.0	38.0	41.0	35.0	41.0
46-47	38.85103737849302	40.0	38.0	41.0	35.0	41.0
48-49	38.82724096368352	40.0	38.0	41.0	35.0	41.0
50-51	38.70392831096977	40.0	38.0	41.0	35.0	41.0
52-53	38.51961006583994	40.0	38.0	41.0	35.0	41.0
54-55	38.29470216284555	40.0	37.0	41.0	34.0	41.0
56-57	38.1180768696117	40.0	37.0	41.0	34.0	41.0
58-59	37.92541568700163	39.0	36.0	41.0	34.0	41.0
60-61	37.66610961049639	39.0	36.0	41.0	34.0	41.0
62-63	37.4661446652611	39.0	35.0	40.5	33.5	41.0
64-65	37.16542902528778	38.0	35.0	40.0	33.0	41.0
66-67	36.90029302226888	37.0	35.0	40.0	33.0	41.0
68-69	36.618461598076635	37.0	35.0	39.5	33.0	41.0
70-71	36.1645176491437	36.0	35.0	39.0	32.5	41.0
72-73	35.90789455652822	36.0	35.0	39.0	32.0	40.5
74-75	35.55897575330523	35.0	35.0	38.5	32.0	40.0
76-77	33.81754925264184	34.5	32.0	36.0	29.0	39.0
78-79	34.84506199476542	35.0	34.0	37.0	31.5	39.0
80-81	34.80164162749717	35.0	34.0	36.0	32.0	39.0
82-83	34.57108615982398	35.0	34.0	36.0	32.0	37.0
84-85	34.3629821716764	35.0	34.0	35.5	31.0	37.0
86-87	34.209280364856845	35.0	34.0	35.0	31.0	37.0
88-89	34.0716281293777	35.0	34.0	35.0	31.0	36.0
90-91	34.03359965024389	35.0	34.0	35.0	31.0	36.0
92-93	33.93231994746502	35.0	34.0	35.0	31.0	36.0
94-95	33.79515670215696	35.0	34.0	35.0	31.0	36.0
96-97	33.832140042576654	35.0	34.0	35.0	31.0	35.0
98-99	33.87055626121746	35.0	34.0	35.0	31.0	35.0
100-101	32.62111594944853	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	9.0
20	24.0
21	81.0
22	301.0
23	967.0
24	2610.0
25	5817.0
26	10984.0
27	18365.0
28	27963.0
29	39142.0
30	53485.0
31	72011.0
32	98435.0
33	141568.0
34	233245.0
35	406652.0
36	868112.0
37	1859242.0
38	2159821.0
39	1056509.0
40	26097.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.64490221693579	19.46870700469015	12.539128688638371	24.34726208973569
2	15.86829049027786	20.678614454300906	39.55829647928187	23.89479857613936
3	18.476334515301332	26.328386578379163	29.6440115739662	25.5512673323533
4	12.801815336737254	17.078953280836483	38.0221765598273	32.097054822598956
5	13.462643191215346	37.85208940554464	35.115739171693896	13.569528231546126
6	31.098450716178906	37.020936958661146	18.2951367956708	13.58547552948915
7	27.447464435557677	31.86242743532001	23.140459688924896	17.549648440197412
8	26.000654386586007	34.978883535144895	20.819858557036625	18.200603521232473
9	26.777223276621704	15.416596059558508	20.648808716871144	37.15737194694864
10-11	25.20032360754917	25.878831877857216	28.266235415877922	20.654609098715696
12-13	25.883949831188318	23.45144043669825	28.3970293210612	22.267580411052233
14-15	23.072366203432324	24.553371270056477	25.90673282457624	26.46752970193496
16-17	22.668790151608974	27.637256315487203	26.519630679687932	23.17432285321589
18-19	22.70147417736023	26.9913920062315	28.196662233011615	22.110471583396656
20-21	23.472285319238264	26.230302374708863	27.874454880616366	22.42295742543651
22-23	23.482680761442737	26.10789635820221	27.609272470169525	22.800150410185523
24-25	23.069199164591815	26.359358723713484	27.713630514731197	22.857811596963508
26-27	22.9899739252539	26.59938485415296	27.679620298343476	22.731020922249666
28-29	22.797381006976387	26.671709446508245	27.70811666243489	22.822792884080478
30-31	23.092739165539175	26.585161322550512	27.50164473859019	22.820454773320126
32-33	22.90051214121695	26.785923378805414	27.427036565945865	22.886527914031767
34-35	22.891173932680406	26.79293570772649	27.579545183566246	22.73634517602686
36-37	23.04355399633156	26.682017073646396	27.449666719462872	22.824762210559168
38-39	23.0132517303919	26.733382271513527	27.464330489593003	22.78903550850157
40-41	22.93785959517715	26.549540709061397	27.68124043670264	22.831359259058807
42-43	23.03291630484953	26.729572270709586	27.197955842963122	23.039555581477767
44-45	23.026092829573333	27.100881946569018	26.879945836081333	22.993079387776323
46-47	23.15530887664982	26.930644765890747	26.78051741745408	23.133528940005352
48-49	23.288126326003333	26.79907896915394	26.609474450250087	23.30332025459264
50-51	22.932515581534425	26.872688418854114	26.70688068545098	23.48791531416048
52-53	23.05427181538141	26.99960053619726	26.474802991715467	23.47132465670586
54-55	23.205672454426047	26.8615195826031	26.450208840546598	23.482599122424258
56-57	23.388226126830165	26.79017666401002	26.52138474177812	23.3002124673817
58-59	23.19604564318239	26.798541296982233	26.73228348067521	23.27312957916017
60-61	23.318929506578712	26.96269202730532	26.313168583796497	23.40520988231947
62-63	23.298505879825008	26.94503942848617	26.32335632632124	23.433098365367588
64-65	23.133549109477062	26.87263171229167	26.406460021475592	23.58735915675567
66-67	23.169360473894404	26.884112587904124	26.243271295624254	23.70325564257722
68-69	23.158271648874525	26.992952700830212	26.368095783337765	23.480679866957495
70-71	23.20523843677568	26.62334482310641	26.330112518775344	23.84130422134257
72-73	23.254932178061328	26.837613515532947	26.333196827312698	23.574257479093024
74-75	23.050322361288362	27.016918336566064	26.45832500996302	23.47443429218256
76-77	23.28600183799454	26.85437490292607	26.18396067532834	23.675662583751052
78-79	23.2420729297106	26.789959557056775	26.299732995593438	23.66823451763919
80-81	23.134007307277805	26.98722716077169	26.26575066025675	23.613014871693757
82-83	23.435197237103374	26.762807273363116	26.46501095456777	23.336984534965744
84-85	23.519128841597755	26.757931438162192	26.1590539733714	23.56388574686865
86-87	23.129843602582778	27.04600744161139	26.42771164508409	23.396437310721733
88-89	23.35743660709065	26.66061723857941	26.392371451495034	23.589574702834906
90-91	23.438733342619678	26.569996841622416	26.38536186384076	23.605907951917146
92-93	23.438012951433578	26.733270027387846	26.34286145917161	23.485855562006968
94-95	23.234954540265047	26.767767789103463	26.46141077016808	23.53586690046341
96-97	23.17493905754888	26.653419858405115	26.547380154757107	23.624260929288905
98-99	23.526947492638037	27.282286957101327	26.537680772381016	22.65308477787962
100-101	28.277691271216156	34.24690200962133	26.847122777806415	10.628283941356097
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1267.0
1	1302.5
2	2322.5
3	3513.5
4	5046.0
5	8717.5
6	11570.0
7	12903.0
8	13217.0
9	11963.5
10	9940.5
11	8291.5
12	9158.0
13	13012.5
14	21072.5
15	31030.0
16	38086.5
17	41425.5
18	41056.0
19	37434.0
20	32920.5
21	29010.5
22	27256.0
23	29215.0
24	34426.5
25	42375.0
26	52243.0
27	63500.5
28	74439.0
29	86939.5
30	99850.5
31	111631.0
32	125889.5
33	139990.5
34	152329.0
35	168805.5
36	189658.0
37	205956.0
38	213331.5
39	216169.0
40	220283.0
41	224656.5
42	229535.0
43	240296.5
44	255895.5
45	274152.0
46	285829.0
47	293960.5
48	308358.0
49	318923.5
50	327642.5
51	335072.5
52	330665.5
53	326377.5
54	322223.5
55	306965.5
56	287236.0
57	265938.0
58	239985.5
59	209362.5
60	175471.5
61	142249.5
62	113362.0
63	88808.0
64	68602.5
65	51033.0
66	37544.0
67	28356.0
68	21319.0
69	15778.5
70	11509.5
71	8298.5
72	5998.0
73	4305.5
74	3025.0
75	1991.5
76	1270.5
77	810.0
78	540.0
79	394.5
80	289.5
81	190.5
82	125.0
83	79.0
84	52.5
85	35.5
86	23.0
87	20.5
88	16.5
89	10.5
90	6.5
91	3.5
92	1.5
93	1.0
94	1.0
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0018357845528897295
3	4.377640087660125E-4
4	0.0
5	1.4121419637613306E-5
6	5.648567855045322E-4
7	0.0
8	0.0
9	1.4121419637613306E-5
10-11	3.389140713027193E-4
12-13	4.1658187930959244E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.945354189446204E-5
28-29	0.0
30-31	5.668455040613417E-5
32-33	2.7015719238426876E-4
34-35	2.8558279523585068E-5
36-37	7.757749614752309E-4
38-39	2.5372782355390186E-4
40-41	8.05599472932883E-5
42-43	0.0
44-45	1.4982272226388126E-5
46-47	1.5193535636779692E-5
48-49	7.700926213498477E-6
50-51	1.4043548889065559E-4
52-53	0.0
54-55	8.142487788663167E-4
56-57	3.231933572130132E-5
58-59	8.183007395965745E-6
60-61	0.0010445055526246767
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	7.18452224004782E-5
74-75	1.820144430280687E-5
76-77	2.766952077773489E-5
78-79	4.6755906860737344E-5
80-81	0.0
82-83	0.0
84-85	2.9291159791282912E-5
86-87	5.842193250444823E-4
88-89	1.6074628068269748E-4
90-91	0.0
92-93	2.0751507545144386E-5
94-95	1.055166310565623E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	110.0
20-21	480.0
22-23	1075.0
24-25	1736.0
26-27	4228.0
28-29	11639.0
30-31	22781.0
32-33	27841.0
34-35	37288.0
36-37	58645.0
38-39	71235.0
40-41	70760.0
42-43	77122.0
44-45	90919.0
46-47	90985.0
48-49	86086.0
50-51	77701.0
52-53	69152.0
54-55	74126.0
56-57	77839.0
58-59	77541.0
60-61	79383.0
62-63	73180.0
64-65	74656.0
66-67	78365.0
68-69	80162.0
70-71	80309.0
72-73	74065.0
74-75	72972.0
76-77	73071.0
78-79	76998.0
80-81	77858.0
82-83	72373.0
84-85	71469.0
86-87	71849.0
88-89	75919.0
90-91	80526.0
92-93	80162.0
94-95	84124.0
96-97	125185.0
98-99	544400.0
100-101	4005126.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.89198993886282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.00726797952358	68.64176510048516
2	8.047096944449986	12.697029823572045
3	2.4112256729676913	5.706791745962845
4	1.0561994235652943	3.333026971893837
5	0.503801599738089	1.9872955368860161
6	0.30001209015219105	1.4201130478694317
7	0.1887522596261911	1.0423728957156966
8	0.11984448266408017	0.7563815776450267
9	0.07917059588286465	0.562133326846026
>10	0.2799700160147408	3.405358088236179
>50	0.005628218097196975	0.29462848362803007
>100	0.0010307173182082185	0.1531034012597287
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	3.530354909403326E-5	0.0
22-23	0.0	0.0	0.0	7.060709818806652E-5	0.0
24-25	0.0	0.0	0.0	1.4121419637613304E-4	0.0
26-27	0.0	0.0	0.0	2.047605847453929E-4	0.0
28-29	0.0	0.0	0.0	0.0011720778299219043	0.0
30-31	0.0	0.0	0.0	0.004017543886900985	0.0
32-33	0.0	0.0	0.0	0.009129497795717001	0.0
34-35	0.0	0.0	0.0	0.01491221913731965	0.0
36-37	0.0	0.0	0.0	0.023250917433330305	0.0
38-39	0.0	0.0	0.0	0.039561157114773676	0.0
40-41	0.0	0.0	0.0	0.0788469465466139	0.0
42-43	7.060709818806652E-6	0.0	0.0	0.09458526873273392	7.060709818806652E-6
44-45	1.4121419637613304E-5	0.0	0.0	0.11426346699774806	1.4121419637613304E-5
46-47	4.2364258912839915E-5	0.0	0.0	0.13625757808333078	1.4121419637613304E-5
48-49	4.2364258912839915E-5	0.0	0.0	0.15608405125453984	1.4121419637613304E-5
50-51	4.2364258912839915E-5	0.0	0.0	0.1774568198760676	1.4121419637613304E-5
52-53	4.2364258912839915E-5	0.0	0.0	0.20338374633072562	1.4121419637613304E-5
54-55	4.2364258912839915E-5	0.0	0.0	0.2539807928922941	1.4121419637613304E-5
56-57	4.2364258912839915E-5	0.0	0.0	0.3083270763676489	1.4121419637613304E-5
58-59	4.2364258912839915E-5	0.0	0.0	0.3621579280262308	2.824283927522661E-5
60-61	4.2364258912839915E-5	0.0	0.0	0.398414672945803	2.824283927522661E-5
62-63	4.2364258912839915E-5	0.0	0.0	0.41579107980988617	2.824283927522661E-5
64-65	4.2364258912839915E-5	0.0	0.0	0.43221429084843044	2.824283927522661E-5
66-67	4.2364258912839915E-5	0.0	0.0	0.4403905928186085	2.824283927522661E-5
68-69	4.2364258912839915E-5	0.0	0.0	0.4481644343291147	2.824283927522661E-5
70-71	4.2364258912839915E-5	0.0	0.0	0.45569821170578134	2.824283927522661E-5
72-73	4.2364258912839915E-5	0.0	0.0	0.46129735459209503	2.824283927522661E-5
74-75	4.2364258912839915E-5	0.0	0.0	0.4656185090012047	2.824283927522661E-5
76-77	4.2364258912839915E-5	0.0	0.0	0.46972784211575014	2.824283927522661E-5
78-79	4.2364258912839915E-5	0.0	0.0	0.4734700183197177	2.824283927522661E-5
80-81	4.2364258912839915E-5	0.0	0.0	0.4751081029976808	2.824283927522661E-5
82-83	4.2364258912839915E-5	0.0	0.0	0.4756800204930042	2.824283927522661E-5
84-85	4.2364258912839915E-5	0.0	0.0	0.47617427018032066	2.824283927522661E-5
86-87	4.2364258912839915E-5	0.0	0.0	0.47640021289452245	2.824283927522661E-5
88-89	4.2364258912839915E-5	0.0	0.0	0.4764425771534353	2.824283927522661E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9150	0.0	44.797935	1
GTATCAA	16090	0.0	34.120365	1
ATCAACG	19810	0.0	26.884235	3
CAACGCA	20060	0.0	26.528547	5
TCAACGC	20225	0.0	26.454128	4
TATCAAC	20710	0.0	26.132341	2
AACGCAG	21345	0.0	25.12389	6
GTACATG	25125	0.0	24.349226	1
TACATGG	25280	0.0	23.664322	2
ACATGGG	25485	0.0	23.071138	3
ACGCAGA	23350	0.0	22.808424	7
GTACTAG	1845	0.0	22.01671	1
CGCAGAG	24595	0.0	21.65386	8
CATGGGG	18740	0.0	21.587967	4
GCAGAGT	28945	0.0	18.385435	9
GAGTACT	14860	0.0	17.933424	12-13
AGAGTAC	25755	0.0	17.101982	10-11
CAGAGTA	27425	0.0	16.995646	10-11
CTAGTAC	2210	0.0	16.894938	3
AGTACTT	15975	0.0	16.514786	12-13
>>END_MODULE