FastQCFastQC Report
Fri 27 May 2016
SRR2140107_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140107_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5938725
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87450.044.8989941
GTATCAA154150.033.6736151
ATCAACG184750.027.5491963
CAACGCA186650.027.182655
TCAACGC192400.026.4752564
TATCAAC195800.026.221832
AACGCAG195850.026.0913836
ACGCAGA221700.022.8851687
CGCAGAG224350.022.5968468
TACATGG217300.022.5306152
ACATGGG217750.021.94573
GTACATG229900.021.7877751
CATGGGG150500.019.1642514
GTACTAG18650.019.0604881
GCAGAGT268250.018.8837369
GAGTACT147950.018.51117112-13
CAGAGTA253100.017.5958110-11
TATACCG6700.016.8815925
AGAGTAC247200.016.83106410-11
GTACTTT164750.016.70956414-15
Produced by FastQC (version 0.11.5)