##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140107_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5938725
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76671490934502	34.0	31.0	34.0	31.0	34.0
2	32.96818088057622	34.0	33.0	34.0	31.0	34.0
3	33.07528602519901	34.0	33.0	34.0	31.0	34.0
4	36.45479863101929	37.0	37.0	37.0	35.0	37.0
5	36.31648055769546	37.0	37.0	37.0	35.0	37.0
6	36.34593418621	37.0	37.0	37.0	35.0	37.0
7	36.35386854922563	37.0	37.0	37.0	35.0	37.0
8	36.36959381011918	37.0	37.0	37.0	35.0	37.0
9	38.280552475489266	39.0	39.0	39.0	37.0	39.0
10-11	38.18763926600407	39.0	39.0	39.0	37.0	39.0
12-13	38.1727536634547	39.0	39.0	39.0	37.0	39.0
14-15	39.66883868170356	41.0	40.0	41.0	37.5	41.0
16-17	39.64991399669121	41.0	40.0	41.0	37.0	41.0
18-19	39.69843788018472	41.0	40.0	41.0	37.5	41.0
20-21	39.72101240886413	41.0	40.0	41.0	37.5	41.0
22-23	39.66984961460419	41.0	40.0	41.0	37.0	41.0
24-25	39.64446225991031	41.0	40.0	41.0	37.0	41.0
26-27	39.52787338125505	41.0	39.0	41.0	37.0	41.0
28-29	39.463596327771356	41.0	39.0	41.0	37.0	41.0
30-31	39.38419653585902	40.0	39.0	41.0	36.5	41.0
32-33	39.28968561755346	40.0	39.0	41.0	36.0	41.0
34-35	39.25373287136097	40.0	39.0	41.0	35.5	41.0
36-37	39.21301227899663	40.0	39.0	41.0	35.0	41.0
38-39	39.15188663582473	40.0	39.0	41.0	35.0	41.0
40-41	39.08730984252776	40.0	39.0	41.0	35.0	41.0
42-43	39.07595180810393	40.0	39.0	41.0	35.0	41.0
44-45	39.01483244045225	40.0	39.0	41.0	35.0	41.0
46-47	38.90232438905386	40.0	38.0	41.0	35.0	41.0
48-49	38.89300058720471	40.0	38.0	41.0	35.0	41.0
50-51	38.78507385227267	40.0	38.0	41.0	35.0	41.0
52-53	38.62430477603897	40.0	38.0	41.0	35.0	41.0
54-55	38.42326199867146	40.0	37.5	41.0	34.5	41.0
56-57	38.27194373235911	40.0	37.0	41.0	34.0	41.0
58-59	38.108958323018385	40.0	36.5	41.0	34.0	41.0
60-61	37.87395804374427	39.0	36.0	41.0	34.0	41.0
62-63	37.705169772537516	39.0	35.5	41.0	34.0	41.0
64-65	37.434608230802304	39.0	35.0	40.5	33.5	41.0
66-67	37.194621817801774	38.0	35.0	40.0	33.0	41.0
68-69	36.93766218007579	37.0	35.0	40.0	33.0	41.0
70-71	36.497379143828624	37.0	35.0	39.5	33.0	41.0
72-73	36.24236620587902	36.0	35.0	39.0	33.0	41.0
74-75	35.88828097916301	36.0	35.0	39.0	32.5	40.5
76-77	34.09968197967673	34.5	33.0	36.5	30.0	39.0
78-79	35.104065374918875	35.0	34.0	37.0	32.0	39.0
80-81	35.046046075377674	35.0	34.0	37.0	32.0	39.0
82-83	34.78841186153643	35.0	34.0	36.0	32.0	38.0
84-85	34.55496207624836	35.0	34.0	36.0	32.0	37.0
86-87	34.37964395903341	35.0	34.0	36.0	32.0	37.0
88-89	34.22482563581394	35.0	34.0	35.0	32.0	36.5
90-91	34.16293958479593	35.0	34.0	35.0	32.0	36.0
92-93	34.04593554255528	35.0	34.0	35.0	31.5	36.0
94-95	33.902441151860415	35.0	34.0	35.0	31.0	36.0
96-97	33.93294239566381	35.0	34.0	35.0	31.0	35.5
98-99	33.958962793386206	35.0	34.0	35.0	31.0	35.0
100-101	32.73382614743633	34.0	32.0	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	3.0
19	11.0
20	18.0
21	79.0
22	216.0
23	700.0
24	1943.0
25	4297.0
26	8013.0
27	13538.0
28	20732.0
29	30202.0
30	41577.0
31	56878.0
32	78796.0
33	113350.0
34	185632.0
35	318871.0
36	668893.0
37	1458751.0
38	1909779.0
39	1002050.0
40	24393.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.126647554820266	19.933672631751765	12.882478983283448	24.057200830144517
2	15.404683427625374	21.079280414613308	40.306605449528696	23.209430708232624
3	18.11554400044454	26.73192235998175	30.29314545800505	24.85938818156866
4	12.179314583517506	17.64688548467895	39.250243107737774	30.923556824065773
5	12.931883527188075	38.39031105161461	35.60398907172836	13.073816349468952
6	29.88045856546911	37.9992469724825	18.926518187332096	13.193776274716292
7	26.646611856922153	32.70644793284754	23.47744675835301	17.169493451877297
8	25.478078207022552	35.38857246294449	21.340641299268782	17.792708030764178
9	26.611040791092634	15.582541903368789	21.12327852300907	36.68313878252951
10-11	24.666798064594403	26.292893726719978	28.900314689898227	20.139993518787392
12-13	25.469635531406613	24.113253049783108	28.815821843867877	21.6012895749424
14-15	22.559160425848983	25.32167258123587	26.64360616125515	25.475560831659994
16-17	21.971315391771803	28.07805547487045	27.29875520418945	22.651873929168296
18-19	21.658967202556102	27.617712556146312	29.177634930056534	21.54568531124105
20-21	22.781336258401037	26.800615705646763	28.81923068114921	21.59881735480299
22-23	22.559844986683807	26.82525409558543	28.570029608102498	22.04487130962827
24-25	22.133682234465166	26.903876654601603	28.96021157745515	22.00222953347808
26-27	22.240721151015087	27.133236178153698	28.61726062661805	22.00878204421316
28-29	21.94410180735595	27.434669911687465	28.625903060343877	21.995325220612706
30-31	22.377265257535534	27.14679424892772	28.454563692904085	22.021376800632662
32-33	22.002072831379262	27.290091470745875	28.4712185776855	22.236617120189358
34-35	22.242667830626168	27.43545657657854	28.548424675009702	21.77345091778559
36-37	22.36932963920478	27.25506773784384	28.40082238542389	21.97478023752749
38-39	22.11944371161226	27.275331273853396	28.54295665835918	22.062268356175164
40-41	22.339559038627854	27.16510539576032	28.5296555541146	21.965680011497223
42-43	22.350684510522985	27.26640089200355	28.103319604560234	22.279594992913225
44-45	22.261721623761126	27.739658737040905	27.8309244163884	22.167695222809567
46-47	22.416288366927958	27.526584193807107	27.547619367302534	22.509508071962397
48-49	22.49521852513365	27.599298030705683	27.46146950197167	22.444013942188995
50-51	22.377462046291285	27.551915786866093	27.334378618580157	22.736243548262465
52-53	22.439037277444587	27.586403894411553	27.181643672329912	22.792915155813944
54-55	22.57308134624862	27.62037844052098	27.1416250081037	22.664915205126697
56-57	22.678154302886906	27.665060153148453	27.01130293745491	22.64548260650973
58-59	22.511053294540705	27.707150258614195	27.300439905519003	22.481356541326093
60-61	22.683530398820988	27.765392443533045	27.077260919654183	22.473816237991787
62-63	22.696563221787404	27.56773863395643	27.053015129835174	22.68268301442099
64-65	22.590035341396604	27.642510405490405	27.05404696780272	22.713407285310268
66-67	22.55551227113362	27.698805612815413	26.93116778040814	22.81451433564283
68-69	22.555481488454177	27.5314352944893	27.083265296190497	22.829817920866024
70-71	22.65850677015127	27.29318261650282	27.05457208068149	22.993738532664423
72-73	22.638006696295207	27.439295913024086	27.216405164040967	22.70629222663974
74-75	22.550690561898413	27.559274936517152	27.189110407414276	22.700924094170162
76-77	22.687205057430326	27.431819824400065	27.01543098063498	22.865544137534627
78-79	22.503982596639208	27.4397688322884	27.061105889918867	22.99514268115352
80-81	22.431645661616432	27.378634696272137	27.267457607235357	22.922262034876073
82-83	22.853878895876736	27.17155825808744	27.382559004995894	22.592003841039933
84-85	22.943423330802485	27.26660746615963	27.09297220220341	22.696997000834482
86-87	22.61414870081353	27.405653638176315	27.247969479790413	22.732228181219742
88-89	22.960187202172	27.198851315931027	27.02416079587041	22.81680068602656
90-91	22.906514185893755	27.09505454633782	27.184168657944515	22.814262609823906
92-93	23.059757314525665	27.090109680046297	27.175473775884406	22.67465922954363
94-95	22.597955576272806	27.059844715922633	27.495658973638275	22.846540734166286
96-97	22.704142120246352	27.18704475921695	27.331397837699477	22.777415282837215
98-99	22.971791393710273	27.718056282651553	27.31100917711762	21.999143146520556
100-101	27.291118640743694	34.4899553191194	28.103547449573803	10.1153785905631
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	757.0
1	878.5
2	1929.5
3	3043.5
4	4508.0
5	7748.0
6	10044.5
7	11181.0
8	11566.0
9	10609.0
10	8970.5
11	7738.0
12	8705.0
13	12546.5
14	20891.5
15	31087.0
16	38311.0
17	41812.5
18	41512.0
19	37855.5
20	33010.5
21	28918.0
22	26801.5
23	28030.0
24	32959.5
25	41164.0
26	52084.5
27	63287.5
28	74313.0
29	88210.0
30	101761.0
31	113641.5
32	127249.5
33	141408.0
34	154577.5
35	168459.0
36	183346.0
37	198370.5
38	207912.0
39	211729.0
40	214430.5
41	214760.0
42	216835.5
43	225392.0
44	236855.5
45	248778.5
46	257553.5
47	263426.0
48	266630.5
49	265000.0
50	258944.0
51	252990.5
52	250655.0
53	248450.5
54	244143.0
55	232380.5
56	215769.5
57	194724.0
58	172334.5
59	147671.5
60	118191.5
61	93437.5
62	74061.0
63	57398.0
64	42633.5
65	30778.5
66	22677.5
67	17888.0
68	14185.0
69	10802.0
70	8126.0
71	5563.5
72	3967.0
73	2745.5
74	1876.5
75	1362.0
76	898.5
77	542.0
78	351.0
79	246.5
80	169.5
81	114.0
82	77.5
83	53.0
84	41.5
85	27.0
86	17.0
87	15.5
88	11.5
89	6.5
90	4.0
91	2.5
92	1.5
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0013976063885766726
3	5.893520915684763E-4
4	0.0
5	0.0
6	5.219975668177934E-4
7	0.0
8	0.0
9	3.367726237534151E-5
10-11	3.6203057053492123E-4
12-13	4.0412714850409807E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.897039046148879E-5
28-29	0.0
30-31	4.225397223254862E-5
32-33	2.2053153698521686E-4
34-35	1.7043527464792332E-5
36-37	6.691595433346433E-4
38-39	1.8200398346051802E-4
40-41	7.891621728265159E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	1.8549021353633385E-5
50-51	9.411895808054551E-6
52-53	0.0
54-55	5.41048739892654E-4
56-57	7.837282343974405E-5
58-59	0.0
60-61	8.37074619857185E-4
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	6.6018838475559E-5
74-75	2.2324615310600697E-5
76-77	1.13281496475756E-5
78-79	4.600167262081649E-5
80-81	0.0
82-83	0.0
84-85	2.4110979947380198E-5
86-87	4.896999571634963E-4
88-89	9.949613911450675E-5
90-91	2.530212636539762E-5
92-93	0.0
94-95	1.3115872172709804E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	98.0
20-21	399.0
22-23	838.0
24-25	1599.0
26-27	3777.0
28-29	10466.0
30-31	20779.0
32-33	25730.0
34-35	34422.0
36-37	54425.0
38-39	67906.0
40-41	65518.0
42-43	72334.0
44-45	84277.0
46-47	84386.0
48-49	80787.0
50-51	71885.0
52-53	66332.0
54-55	70975.0
56-57	72602.0
58-59	72828.0
60-61	71222.0
62-63	65918.0
64-65	66336.0
66-67	70439.0
68-69	71065.0
70-71	70935.0
72-73	65237.0
74-75	65364.0
76-77	65263.0
78-79	68154.0
80-81	68976.0
82-83	64177.0
84-85	63259.0
86-87	63373.0
88-89	66940.0
90-91	70776.0
92-93	69255.0
94-95	70801.0
96-97	100061.0
98-99	432964.0
100-101	3225847.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.35139892204771
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.0211034221599	71.60639898054949
2	7.561806848127935	12.303271309470556
3	2.2884404163241707	5.5850348765317355
4	0.9014360377396314	2.933323308354672
5	0.4726744367844749	1.9226363333554017
6	0.26278843052730194	1.2826923866355218
7	0.15044032665142995	0.8566971719168549
8	0.0955996025365473	0.622172912219189
9	0.0687976712914839	0.5037108121927274
>10	0.1746095037916144	2.0200868451292453
>50	0.001247695402376126	0.0676806208997367
>100	8.311319325798226E-4	0.16131155108926049
>500	2.2447673054469872E-4	0.1349828916555842
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.6838631187670754E-5	1.6838631187670754E-5	0.0	0.0	0.0
3	1.6838631187670754E-5	1.6838631187670754E-5	0.0	0.0	0.0
4	1.6838631187670754E-5	3.367726237534151E-5	0.0	0.0	0.0
5	5.0515893563012266E-5	3.367726237534151E-5	0.0	0.0	0.0
6	8.419315593835377E-5	3.367726237534151E-5	0.0	0.0	0.0
7	1.515476806890368E-4	5.0515893563012266E-5	0.0	0.0	0.0
8	1.6838631187670753E-4	5.0515893563012266E-5	0.0	0.0	0.0
9	1.6838631187670753E-4	5.0515893563012266E-5	0.0	0.0	0.0
10-11	1.6838631187670753E-4	5.0515893563012266E-5	0.0	0.0	0.0
12-13	2.862567301904028E-4	5.0515893563012266E-5	0.0	0.0	0.0
14-15	3.5361125494108586E-4	5.0515893563012266E-5	0.0	0.0	0.0
16-17	3.7886920172259197E-4	5.893520915684764E-5	0.0	0.0	0.0
18-19	4.546430420671104E-4	6.735452475068302E-5	0.0	1.6838631187670754E-5	0.0
20-21	5.051589356301227E-4	6.735452475068302E-5	0.0	1.6838631187670754E-5	0.0
22-23	5.051589356301227E-4	6.735452475068302E-5	0.0	3.367726237534151E-5	0.0
24-25	7.072225098821717E-4	8.419315593835377E-5	0.0	3.367726237534151E-5	0.0
26-27	7.156418254760071E-4	8.419315593835377E-5	0.0	8.419315593835377E-5	0.0
28-29	8.587701905712085E-4	8.419315593835377E-5	0.0	6.314486695376532E-4	0.0
30-31	9.429633465095623E-4	8.419315593835377E-5	0.0	0.0024331822066184236	0.0
32-33	9.513826621033977E-4	8.419315593835377E-5	0.0	0.004706397416953976	0.0
34-35	9.850599244787392E-4	1.0103178712602453E-4	0.0	0.007501610194107321	0.0
36-37	0.0011113496583862697	1.0103178712602453E-4	0.0	0.011627074835086655	0.0
38-39	0.0011955428143246235	1.0103178712602453E-4	0.0	0.01897713734850494	0.0
40-41	0.0011955428143246235	1.0103178712602453E-4	0.0	0.027076518949774572	0.0
42-43	0.0012460587078876359	1.094511027198599E-4	0.0	0.034460258725568196	0.0
44-45	0.0013976063885766726	1.1787041831369528E-4	0.0	0.04258489827361934	0.0
46-47	0.0016080892784225572	1.1787041831369528E-4	0.0	0.051181019494925255	0.0
48-49	0.001650185856391734	1.515476806890368E-4	0.0	0.06018968718032911	0.0
50-51	0.001650185856391734	1.936442586582137E-4	0.0	0.0683732619375371	0.0
52-53	0.001650185856391734	2.0206357425204906E-4	0.0	0.07670838437543412	0.0
54-55	0.0016670244875794046	2.0206357425204906E-4	0.0	0.0849845716041743	0.0
56-57	0.0017512176435177584	2.0206357425204906E-4	0.0	0.09332811335766517	0.0
58-59	0.0017512176435177584	2.0206357425204906E-4	0.0	0.10187371868540807	0.0
60-61	0.0017512176435177584	2.189022054397198E-4	0.0	0.10995626165549002	0.0
62-63	0.0017764755902992646	2.189022054397198E-4	0.0	0.11771045131741241	0.0
64-65	0.0017848949058930999	2.189022054397198E-4	0.0	0.12560776934443	0.0
66-67	0.0018690880618314537	2.189022054397198E-4	0.0	0.13257896265612568	0.0
68-69	0.0018690880618314537	2.189022054397198E-4	0.0	0.14018160463735901	0.0
70-71	0.001944861902175972	2.525794678150613E-4	0.0	0.1473548615233068	0.0
72-73	0.0020122164269266553	3.030953613780736E-4	0.0	0.1536188323251203	0.0
74-75	0.0020458936893019966	3.1993399256574433E-4	0.0	0.1584767774227633	0.0
76-77	0.002054313004895832	3.283533081595797E-4	0.0	0.1630737237369974	0.0
78-79	0.002054313004895832	3.536112549410858E-4	0.0	0.16705606001288154	0.0
80-81	0.0021974413699910335	3.704498861287566E-4	0.0	0.16881569697199317	0.0
82-83	0.00223953794796021	3.704498861287566E-4	0.0	0.16940504906356163	0.0
84-85	0.002247957263554046	3.704498861287566E-4	0.0	0.16991862731478558	0.0
86-87	0.002256376579147881	3.704498861287566E-4	0.0	0.17013752952022532	0.0
88-89	0.002256376579147881	3.704498861287566E-4	0.0	0.17023856130735132	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8745	0.0	44.898994	1
GTATCAA	15415	0.0	33.673615	1
ATCAACG	18475	0.0	27.549196	3
CAACGCA	18665	0.0	27.18265	5
TCAACGC	19240	0.0	26.475256	4
TATCAAC	19580	0.0	26.22183	2
AACGCAG	19585	0.0	26.091383	6
ACGCAGA	22170	0.0	22.885168	7
CGCAGAG	22435	0.0	22.596846	8
TACATGG	21730	0.0	22.530615	2
ACATGGG	21775	0.0	21.9457	3
GTACATG	22990	0.0	21.787775	1
CATGGGG	15050	0.0	19.164251	4
GTACTAG	1865	0.0	19.060488	1
GCAGAGT	26825	0.0	18.883736	9
GAGTACT	14795	0.0	18.511171	12-13
CAGAGTA	25310	0.0	17.59581	10-11
TATACCG	670	0.0	16.881592	5
AGAGTAC	24720	0.0	16.831064	10-11
GTACTTT	16475	0.0	16.709564	14-15
>>END_MODULE