FastQCFastQC Report
Fri 27 May 2016
SRR2140106_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140106_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9054472
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93350.043.802551
GTATCAA159300.032.9572141
TCAACGC186700.027.2703324
ATCAACG188050.027.0079293
CAACGCA190800.026.6407015
AACGCAG197350.025.904796
TATCAAC200400.025.593762
ACGCAGA233950.021.7271797
CGCAGAG239450.021.2106788
GTACATG233700.021.1961521
TACATGG232500.020.7487752
ACATGGG235000.020.0302833
CATGGGG182150.017.6330814
GCAGAGT287900.017.5540439
AGAGTAC253200.016.98228310-11
GAGTACT164950.016.96503312-13
CAGAGTA280700.016.11459710-11
GTGGTAT54900.015.2155861
AGTACTT183050.015.00231412-13
GAGTACA171450.014.7383521
Produced by FastQC (version 0.11.5)