##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140106_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9054472
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.868248860894372	31.0	31.0	34.0	30.0	34.0
2	32.052689654349805	33.0	31.0	34.0	30.0	34.0
3	32.11919170990865	34.0	31.0	34.0	30.0	34.0
4	35.80597731154285	37.0	35.0	37.0	35.0	37.0
5	35.56317728963102	37.0	35.0	37.0	33.0	37.0
6	35.59376670445278	37.0	35.0	37.0	33.0	37.0
7	35.55206189825315	37.0	35.0	37.0	33.0	37.0
8	35.54450750965932	37.0	35.0	37.0	33.0	37.0
9	37.33257289878416	39.0	37.0	39.0	34.0	39.0
10-11	37.18322984487665	39.0	37.0	39.0	33.5	39.0
12-13	37.1881569129597	39.0	37.0	39.0	33.0	39.0
14-15	38.318292607233204	40.0	38.0	41.0	33.5	41.0
16-17	38.25031536902428	40.0	38.0	41.0	33.0	41.0
18-19	38.30830091473031	40.0	38.0	41.0	33.5	41.0
20-21	38.34670065209523	40.0	38.0	41.0	34.0	41.0
22-23	38.33443690933378	40.0	38.0	41.0	34.0	41.0
24-25	38.288266028350336	40.0	38.0	41.0	34.0	41.0
26-27	38.17414384912176	40.0	38.0	41.0	34.0	41.0
28-29	38.001617684315235	40.0	37.0	41.0	33.0	41.0
30-31	37.90539892372828	40.0	37.0	41.0	33.0	41.0
32-33	37.81850102213667	40.0	37.0	41.0	33.0	41.0
34-35	37.75399287000199	40.0	37.0	41.0	33.0	41.0
36-37	37.713880140019185	39.5	37.0	41.0	33.0	41.0
38-39	37.62616030391739	39.0	37.0	41.0	33.0	41.0
40-41	37.609748726775216	39.0	37.0	41.0	33.0	41.0
42-43	37.55920627097437	39.0	37.0	41.0	32.5	41.0
44-45	37.48780805019588	39.0	37.0	41.0	32.0	41.0
46-47	37.435389225472655	39.0	36.0	40.5	32.0	41.0
48-49	37.450786890260616	39.0	36.0	40.0	32.0	41.0
50-51	37.34589016953504	39.0	36.0	40.0	31.5	41.0
52-53	37.15758740543902	39.0	36.0	40.0	31.0	41.0
54-55	36.96524859617265	39.0	35.0	40.0	31.0	41.0
56-57	36.84686967767636	39.0	35.0	40.0	31.0	41.0
58-59	36.65907930208959	38.5	35.0	40.0	31.0	41.0
60-61	36.475851708369305	38.0	35.0	40.0	30.0	41.0
62-63	36.33123613609533	38.0	35.0	40.0	30.0	41.0
64-65	36.07297969421224	37.5	34.0	40.0	30.0	41.0
66-67	35.937383809838	37.0	34.0	40.0	30.0	41.0
68-69	35.66837831084064	37.0	34.0	39.0	30.0	41.0
70-71	35.349087505363045	36.0	34.0	39.0	29.0	40.5
72-73	35.05948315794977	36.0	34.0	39.0	29.0	40.0
74-75	34.71169673290967	35.0	33.5	38.5	29.0	40.0
76-77	32.58542867696796	33.5	30.5	35.5	26.5	38.5
78-79	33.855046220801455	35.0	32.5	37.0	28.5	39.0
80-81	33.85967142672335	35.0	33.0	36.0	29.0	39.0
82-83	33.69164926286008	35.0	33.0	36.0	29.0	38.0
84-85	33.493922548074636	35.0	33.0	36.0	29.0	37.0
86-87	33.25764473441214	35.0	33.0	35.0	29.0	37.0
88-89	33.09832228665346	35.0	32.0	35.0	29.0	36.0
90-91	32.93283902094515	34.0	32.0	35.0	29.0	36.0
92-93	32.896125967939284	34.0	32.0	35.0	29.0	36.0
94-95	32.85429121273597	34.0	32.0	35.0	29.0	35.5
96-97	32.87363984700441	34.0	32.0	35.0	29.0	35.0
98-99	33.0080334746788	34.0	32.0	35.0	29.0	35.0
100-101	31.825798971020333	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	14.0
20	54.0
21	290.0
22	1304.0
23	4652.0
24	12086.0
25	25937.0
26	47466.0
27	76808.0
28	112863.0
29	154094.0
30	202382.0
31	262562.0
32	338608.0
33	447713.0
34	617272.0
35	884449.0
36	1369896.0
37	2019372.0
38	1876255.0
39	594847.0
40	5544.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.71502214596279	20.47531871543697	13.375434812764345	24.434224325835896
2	15.098777560388916	21.700746650674503	40.15390247983447	23.046573309102115
3	18.264691362387858	26.333777681079066	30.429257593210867	24.972273363322202
4	12.545966236352601	17.953205885445335	38.93273953467414	30.568088343527926
5	12.865101355440714	38.12995390565016	36.091049814942274	12.913894923966854
6	30.13816818915559	38.346366303855156	18.658989723531093	12.856475783458162
7	26.454963520232162	32.45716950222713	23.664750817579513	17.423116159961193
8	24.612811443397412	36.835368187795204	21.311589293749076	17.24023107505831
9	25.65608463972278	16.135706201311354	21.194863709336115	37.01334544962975
10-11	24.192522960388825	26.670966576941012	29.345589918330234	19.790920544339926
12-13	24.852122175720755	24.175398484322706	29.459019746568366	21.513459593388166
14-15	21.933748424742404	25.63715618299754	26.733172665019712	25.69592272724035
16-17	21.76087803689039	28.719014687936234	27.437239158574855	22.082868116598522
18-19	21.749023973159684	28.192024276270693	28.8818020024102	21.177149748159422
20-21	22.436333988141875	27.387595556726797	28.75933035904611	21.41674009608522
22-23	22.15156969401437	27.323402139878528	28.632847897056777	21.89218026905032
24-25	21.936397435399527	27.426068193596542	28.705716697685485	21.93181767331844
26-27	21.99153126895671	27.580091275182028	28.553239287413362	21.875138168447897
28-29	21.95603805675948	27.739170147068755	28.460893934623066	21.8438978615487
30-31	22.190158928793508	27.592347464615013	28.398261779507244	21.81923182708423
32-33	21.944302641183842	27.704412176982913	28.423438013018647	21.927847168814594
34-35	22.043222406735268	27.727730951231916	28.47376635671372	21.7552802853191
36-37	22.04909355082934	27.70493157650163	28.380160448718172	21.865814423950862
38-39	21.931228562502795	27.7992956268027	28.423170157318733	21.846305653375772
40-41	22.17408741932774	27.636504624169206	28.28701742264656	21.90239053385649
42-43	22.009421541579684	27.787518120826814	28.136647339120813	22.06641299847269
44-45	22.08908508962697	27.845574284537634	28.0083984191551	22.056942206680297
46-47	22.107635205392796	27.833929700621734	27.819358173910764	22.23907692007471
48-49	22.115632695172106	27.919796210125796	27.790334106476156	22.174236988225935
50-51	22.09625556642123	27.988954363386263	27.72116707023612	22.193622999956382
52-53	22.263103416734825	27.931620713836637	27.575048268277257	22.23022760115128
54-55	22.187249127224415	27.831372357148876	27.651309905802062	22.330068609824647
56-57	22.190985111818822	27.935047258755958	27.619375843906358	22.254591785518862
58-59	22.217841682512084	27.983226334262078	27.597400173872455	22.201531809353384
60-61	22.293680750474785	27.95468992943362	27.588754757682622	22.162874562408973
62-63	22.257886929316868	27.997145776966004	27.5624959471522	22.182471346564924
64-65	22.196984409569673	27.945298424586497	27.549942490991413	22.307774674852418
66-67	22.22792805005363	28.001487899326015	27.445439893281705	22.32514415733865
68-69	22.182592881500828	27.980838314509597	27.543366875821235	22.293201928168337
70-71	22.15229864806234	27.780316559891972	27.587363923635245	22.480020868410445
72-73	22.22252522737849	27.875868366349653	27.529580840786977	22.372025565484876
74-75	22.10424723653738	27.965272758221726	27.546679472873294	22.383800532367605
76-77	22.212034195395898	27.851429830445362	27.4569428603023	22.479593113856442
78-79	22.15203873818841	27.87581075287876	27.46740547473377	22.50474503419906
80-81	22.190872797145694	27.87207532345932	27.509166871333957	22.427885008061025
82-83	22.206060072784666	27.918447250798845	27.547910156169443	22.32758252024705
84-85	22.291404517852513	27.868862323741883	27.514366556123555	22.32536660228205
86-87	22.120448249554265	27.926051763586802	27.576871695329203	22.376628291529734
88-89	22.207729611798342	27.84727492376949	27.542965393269576	22.40203007116259
90-91	22.25509626502724	27.85834379587819	27.52998794951735	22.35657198957722
92-93	22.258795883798577	27.864012611007215	27.57049729274573	22.306694212448473
94-95	22.106123692113826	27.884931823373314	27.6399592109135	22.368985273599357
96-97	22.06962725087884	27.88804968612987	27.66567760042477	22.37664546256652
98-99	22.316235023343655	28.4540935593211	27.726164290302435	21.503507127032805
100-101	26.38079695426518	35.515780127939976	28.339464898514212	9.763958019280635
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1751.0
1	1603.5
2	2271.5
3	3270.0
4	4328.0
5	6943.5
6	9290.5
7	10928.5
8	11990.0
9	11639.5
10	10355.0
11	9107.0
12	9704.0
13	12789.5
14	19981.0
15	29735.5
16	37418.0
17	41842.0
18	43326.5
19	42719.5
20	41452.0
21	40634.0
22	41826.5
23	47061.5
24	56881.0
25	71241.5
26	88957.5
27	107613.5
28	127601.5
29	149038.5
30	168681.5
31	187338.5
32	206925.0
33	224171.0
34	240419.0
35	257099.5
36	275220.0
37	293531.0
38	309324.0
39	321917.0
40	333259.0
41	344914.0
42	354488.0
43	362685.5
44	370361.0
45	377672.5
46	382516.5
47	384949.5
48	384465.5
49	379888.5
50	374843.5
51	366079.5
52	352153.5
53	337441.5
54	319387.0
55	296442.0
56	272878.0
57	248056.0
58	220166.0
59	190248.5
60	158439.5
61	128873.5
62	103334.0
63	80534.5
64	60764.5
65	45521.5
66	34311.5
67	26021.0
68	19964.5
69	14936.0
70	10972.5
71	8077.0
72	5823.5
73	4047.0
74	2751.5
75	1881.0
76	1292.0
77	871.0
78	593.5
79	388.5
80	269.0
81	179.5
82	130.0
83	103.5
84	78.0
85	57.5
86	40.0
87	28.0
88	19.0
89	12.0
90	9.5
91	9.0
92	6.0
93	3.5
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.0863785320668065E-4
3	2.208853260576652E-5
4	0.0
5	0.0
6	0.0
7	1.104426630288326E-5
8	0.0010823380976825595
9	0.0
10-11	2.153631929062236E-4
12-13	4.1968211950956386E-4
14-15	5.52213315144163E-6
16-17	5.52213315144163E-6
18-19	3.699829211465892E-4
20-21	5.5223926671903E-6
22-23	0.0
24-25	2.265015923061939E-4
26-27	0.0
28-29	4.811911239153427E-4
30-31	3.43454969757405E-4
32-33	0.0010718434446582517
34-35	0.00121455477876049
36-37	3.918132743314784E-5
38-39	0.0017527582835863713
40-41	3.975980759433909E-4
42-43	1.4887729058447906E-4
44-45	1.6184126343000708E-4
46-47	4.6741042181511944E-5
48-49	0.0
50-51	0.0
52-53	5.423060480079442E-5
54-55	3.346987622900625E-4
56-57	5.781832277410837E-4
58-59	6.033745807246454E-4
60-61	6.103286739458113E-4
62-63	1.0181825123971675E-4
64-65	5.791437591178945E-5
66-67	1.2370931306497312E-4
68-69	1.1867909377435188E-4
70-71	1.6032043780305155E-4
72-73	0.0
74-75	6.852635780695097E-6
76-77	8.607570649933364E-4
78-79	2.8134343459422787E-5
80-81	8.559995474615726E-5
82-83	0.0
84-85	7.338392812719372E-6
86-87	7.445362209426126E-6
88-89	6.049436600845772E-5
90-91	9.229952025016862E-5
92-93	9.399499648967853E-5
94-95	3.1159786426030025E-4
96-97	9.004898910595516E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	276.0
20-21	860.0
22-23	1747.0
24-25	3532.0
26-27	5655.0
28-29	11333.0
30-31	21807.0
32-33	26562.0
34-35	36794.0
36-37	56993.0
38-39	69011.0
40-41	69587.0
42-43	77674.0
44-45	91077.0
46-47	92257.0
48-49	88550.0
50-51	83175.0
52-53	80055.0
54-55	86285.0
56-57	90408.0
58-59	91142.0
60-61	90755.0
62-63	86967.0
64-65	89163.0
66-67	95035.0
68-69	97982.0
70-71	97576.0
72-73	92763.0
74-75	94097.0
76-77	92062.0
78-79	98809.0
80-81	100520.0
82-83	96300.0
84-85	97112.0
86-87	101569.0
88-89	109708.0
90-91	116294.0
92-93	121964.0
94-95	139451.0
96-97	209169.0
98-99	764214.0
100-101	5178182.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.72929987527242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.43626879944927	75.77872438834005
2	7.265176542566984	12.311466438439055
3	1.8579348000879374	4.722645444760655
4	0.6874521274109855	2.329893498131975
5	0.3002670036441302	1.2720706497206513
6	0.15808473806373072	0.8036645506263438
7	0.08883816285205187	0.5269036738461853
8	0.058308105638730234	0.3952323974258429
9	0.03257367258583635	0.24839500253078417
>10	0.11287639957685373	1.4494620554141744
>50	0.001958434840845155	0.10784203541129524
>100	2.4031818492187545E-4	0.04467068764719017
>500	2.0895097725849523E-5	0.009029177705796797
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.313279890864978E-5	0.0	2.208853260576652E-5	0.0
3	0.0	3.313279890864978E-5	0.0	2.208853260576652E-5	0.0
4	0.0	3.313279890864978E-5	0.0	2.208853260576652E-5	0.0
5	0.0	3.313279890864978E-5	0.0	2.208853260576652E-5	0.0
6	2.0984105975478196E-4	3.313279890864978E-5	0.0	2.208853260576652E-5	0.0
7	2.0984105975478196E-4	3.313279890864978E-5	0.0	3.313279890864978E-5	0.0
8	2.0984105975478196E-4	4.417706521153304E-5	0.0	3.313279890864978E-5	1.104426630288326E-5
9	2.0984105975478196E-4	1.104426630288326E-4	0.0	7.730986412018283E-5	1.104426630288326E-5
10-11	2.153631929062236E-4	1.104426630288326E-4	0.0	1.1596479618027423E-4	1.104426630288326E-5
12-13	2.208853260576652E-4	1.104426630288326E-4	0.0	1.3253119563459912E-4	1.104426630288326E-5
14-15	2.208853260576652E-4	1.656639945432489E-4	0.0	1.3253119563459912E-4	2.761066575720815E-5
16-17	4.196821195095639E-4	1.656639945432489E-4	0.0	1.4357546193748239E-4	3.313279890864978E-5
18-19	4.196821195095639E-4	1.822303939975738E-4	0.0	1.822303939975738E-4	3.313279890864978E-5
20-21	4.196821195095639E-4	1.987967934518987E-4	0.0	2.3192959236054847E-4	4.417706521153304E-5
22-23	4.3072638581244713E-4	2.208853260576652E-4	0.0	2.7058452442063987E-4	5.52213315144163E-5
24-25	4.3072638581244713E-4	2.3192959236054847E-4	0.0	3.202837227836145E-4	5.52213315144163E-5
26-27	4.3072638581244713E-4	2.3192959236054847E-4	0.0	4.252042526610055E-4	5.52213315144163E-5
28-29	4.3072638581244713E-4	2.3192959236054847E-4	0.0	9.056298368364273E-4	5.52213315144163E-5
30-31	4.3072638581244713E-4	2.4297385866343173E-4	0.0	0.0023634729888170176	5.52213315144163E-5
32-33	4.3624851896388876E-4	2.4297385866343173E-4	0.0	0.005085884632477741	5.52213315144163E-5
34-35	4.583370515696553E-4	2.9819519017784804E-4	0.0	0.008349465324979745	6.626559781729956E-5
36-37	4.749034510239802E-4	3.534165216922643E-4	0.0	0.013324907294428654	6.626559781729956E-5
38-39	4.749034510239802E-4	3.644607879951476E-4	0.0	0.02045950332609124	7.178773096874119E-5
40-41	4.8042558417542183E-4	3.865493206009141E-4	0.0	0.028300932401138353	7.730986412018283E-5
42-43	4.8594771732686346E-4	3.975935869037974E-4	0.0	0.03519255457413751	7.730986412018283E-5
44-45	4.914698504783051E-4	4.4177065211533045E-4	0.0	0.04261430152967506	8.283199727162445E-5
46-47	4.969919836297467E-4	4.749034510239802E-4	0.0	0.05143314817252734	8.835413042306608E-5
48-49	5.025141167811884E-4	4.749034510239802E-4	0.0	0.05952307323938934	1.104426630288326E-4
50-51	5.908682472042545E-4	5.466911819927214E-4	0.0	0.06748046711061673	1.104426630288326E-4
52-53	6.184789129614626E-4	5.52213315144163E-4	0.0	0.07469789513955094	1.4357546193748239E-4
54-55	6.240010461129042E-4	5.577354482956046E-4	0.0	0.0822963503559346	1.7670826084613216E-4
56-57	6.295231792643459E-4	5.963903803556961E-4	0.0	0.08941437998814288	1.822303939975738E-4
58-59	6.295231792643459E-4	6.019125135071377E-4	0.0	0.0961292939002959	1.987967934518987E-4
60-61	6.460895787186707E-4	6.12956779810021E-4	0.0	0.1033191112634729	1.987967934518987E-4
62-63	7.841429075047115E-4	6.240010461129042E-4	0.0	0.10965299798817646	1.987967934518987E-4
64-65	7.841429075047115E-4	7.178773096874119E-4	0.0	0.11585987565039685	1.987967934518987E-4
66-67	8.06231440110478E-4	7.178773096874119E-4	0.0	0.12234285997018932	1.987967934518987E-4
68-69	8.172757064133613E-4	7.399658422931784E-4	0.0	0.12920134934427982	1.987967934518987E-4
70-71	8.393642390191278E-4	7.399658422931784E-4	0.0	0.1355518024684377	1.987967934518987E-4
72-73	9.387626357450771E-4	7.510101085960617E-4	0.0	0.14086409456012455	2.3192959236054847E-4
74-75	9.387626357450771E-4	7.786207743532699E-4	0.0	0.14554686347254703	2.3192959236054847E-4
76-77	9.498069020479604E-4	7.896650406561531E-4	0.0	0.14941235667855618	2.3192959236054847E-4
78-79	9.498069020479604E-4	8.06231440110478E-4	0.0	0.15295204402863027	2.3192959236054847E-4
80-81	9.498069020479604E-4	8.06231440110478E-4	0.0	0.15461972824036563	2.429738586634317E-4
82-83	9.498069020479604E-4	8.06231440110478E-4	0.0	0.15526581781908433	2.6506239126919824E-4
84-85	9.498069020479604E-4	8.172757064133613E-4	0.0	0.15619905832167796	2.6506239126919824E-4
86-87	9.55329035199402E-4	8.614527716248943E-4	0.0	0.15660217404173318	2.6506239126919824E-4
88-89	9.829397009566102E-4	9.277183694421939E-4	0.0	0.15671813883791347	2.6506239126919824E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9335	0.0	43.80255	1
GTATCAA	15930	0.0	32.957214	1
TCAACGC	18670	0.0	27.270332	4
ATCAACG	18805	0.0	27.007929	3
CAACGCA	19080	0.0	26.640701	5
AACGCAG	19735	0.0	25.90479	6
TATCAAC	20040	0.0	25.59376	2
ACGCAGA	23395	0.0	21.727179	7
CGCAGAG	23945	0.0	21.210678	8
GTACATG	23370	0.0	21.196152	1
TACATGG	23250	0.0	20.748775	2
ACATGGG	23500	0.0	20.030283	3
CATGGGG	18215	0.0	17.633081	4
GCAGAGT	28790	0.0	17.554043	9
AGAGTAC	25320	0.0	16.982283	10-11
GAGTACT	16495	0.0	16.965033	12-13
CAGAGTA	28070	0.0	16.114597	10-11
GTGGTAT	5490	0.0	15.215586	1
AGTACTT	18305	0.0	15.002314	12-13
GAGTACA	17145	0.0	14.738352	1
>>END_MODULE