FastQCFastQC Report
Fri 27 May 2016
SRR2140105_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140105_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6974677
Sequences flagged as poor quality0
Sequence length19-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82650.044.2504121
GTATCAA140150.034.7053571
ATCAACG169150.027.970223
CAACGCA173850.027.2144435
TCAACGC177200.026.770154
TATCAAC181950.026.344952
AACGCAG183800.025.7864956
ACGCAGA202400.023.3961337
TACATGG225350.022.9088552
GTACATG232550.022.7701641
ACATGGG224500.022.3486483
CGCAGAG212950.022.2370388
CATGGGG178050.019.8886074
GCAGAGT249800.018.9566759
GTACTAG19250.018.7404541
AGAGTAC225800.017.65674810-11
GAGTACT135200.017.37416512-13
CAGAGTA241900.017.18438110-11
GAGTACA162200.016.3869741
CTAGTAC26850.016.2153843
Produced by FastQC (version 0.11.5)