##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140105_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6974677
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.762102245021524	34.0	31.0	34.0	31.0	34.0
2	32.95010966099218	34.0	33.0	34.0	31.0	34.0
3	33.053630870648206	34.0	33.0	34.0	31.0	34.0
4	36.43422340561434	37.0	37.0	37.0	35.0	37.0
5	36.30368245009769	37.0	37.0	37.0	35.0	37.0
6	36.336168112157736	37.0	37.0	37.0	35.0	37.0
7	36.33075352450013	37.0	37.0	37.0	35.0	37.0
8	36.337322144093555	37.0	37.0	37.0	35.0	37.0
9	38.21528452141941	39.0	39.0	39.0	37.0	39.0
10-11	38.12040213475119	39.0	38.0	39.0	37.0	39.0
12-13	38.100578707802526	39.0	38.0	39.0	37.0	39.0
14-15	39.586696057752924	41.0	39.0	41.0	37.0	41.0
16-17	39.55631672979265	41.0	39.0	41.0	37.0	41.0
18-19	39.589728814682026	41.0	39.5	41.0	37.0	41.0
20-21	39.60181045964051	41.0	39.5	41.0	37.0	41.0
22-23	39.549886320362376	41.0	39.0	41.0	37.0	41.0
24-25	39.52406524446481	41.0	39.0	41.0	37.0	41.0
26-27	39.40243354260492	41.0	39.0	41.0	36.5	41.0
28-29	39.331210655384986	40.0	39.0	41.0	36.0	41.0
30-31	39.25952805072174	40.0	39.0	41.0	36.0	41.0
32-33	39.16803940086264	40.0	39.0	41.0	35.5	41.0
34-35	39.126036998686715	40.0	39.0	41.0	35.0	41.0
36-37	39.08701797931627	40.0	39.0	41.0	35.0	41.0
38-39	39.02084839546319	40.0	39.0	41.0	35.0	41.0
40-41	38.95121403129533	40.0	38.0	41.0	35.0	41.0
42-43	38.93577082466882	40.0	38.0	41.0	35.0	41.0
44-45	38.865623401554714	40.0	38.0	41.0	35.0	41.0
46-47	38.74714877091409	40.0	38.0	41.0	35.0	41.0
48-49	38.72716365758242	40.0	38.0	41.0	35.0	41.0
50-51	38.61112739989436	40.0	38.0	41.0	35.0	41.0
52-53	38.431017033817355	40.0	37.5	41.0	34.0	41.0
54-55	38.21860202111222	40.0	37.0	41.0	34.0	41.0
56-57	38.0550108036394	40.0	37.0	41.0	34.0	41.0
58-59	37.8694207494058	39.0	36.0	41.0	34.0	41.0
60-61	37.621281751752804	39.0	36.0	41.0	33.0	41.0
62-63	37.4344723818495	39.0	35.0	40.5	33.0	41.0
64-65	37.13934826329673	38.5	35.0	40.0	33.0	41.0
66-67	36.88454755897207	37.5	35.0	40.0	33.0	41.0
68-69	36.60631813767484	37.0	35.0	40.0	33.0	41.0
70-71	36.15655834129799	36.5	35.0	39.0	32.0	41.0
72-73	35.89762661350643	36.0	35.0	39.0	32.0	40.5
74-75	35.54298772315444	35.5	34.5	38.5	31.5	40.0
76-77	33.756111360402755	34.5	32.0	36.0	29.0	39.0
78-79	34.81999786902573	35.0	34.0	37.0	31.0	39.0
80-81	34.78401659562536	35.0	34.0	36.5	31.5	39.0
82-83	34.54068693418826	35.0	34.0	36.0	31.0	37.5
84-85	34.3177179641326	35.0	34.0	36.0	31.0	37.0
86-87	34.153962314452116	35.0	34.0	35.0	31.0	37.0
88-89	34.006193650334566	35.0	34.0	35.0	31.0	36.0
90-91	33.95408395640192	35.0	34.0	35.0	31.0	36.0
92-93	33.84698281204336	35.0	34.0	35.0	31.0	36.0
94-95	33.70310399695002	35.0	34.0	35.0	31.0	36.0
96-97	33.74061848339258	35.0	34.0	35.0	31.0	35.0
98-99	33.77783487706413	35.0	34.0	35.0	31.0	35.0
100-101	32.52640342266778	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	10.0
20	33.0
21	111.0
22	386.0
23	1105.0
24	2999.0
25	6396.0
26	11965.0
27	20095.0
28	30480.0
29	43464.0
30	59636.0
31	80815.0
32	109389.0
33	157220.0
34	253401.0
35	430496.0
36	883953.0
37	1812764.0
38	2086081.0
39	960486.0
40	23390.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.450771985570086	19.432756527649957	12.485309355544349	24.631162131235612
2	15.786895902865577	20.705498910185586	39.6023589657416	23.905246221207236
3	18.595743155570528	25.9661058260988	29.693862774476138	25.74428824385453
4	12.658421314707477	16.939350739826374	38.35774760608986	32.04448033937629
5	13.346897641281451	37.92754847285401	35.34129250716557	13.38426137869897
6	31.156486266539197	37.28055510790228	18.170948940189636	13.392009685368889
7	27.346413317778012	32.01285163456315	23.041468443628286	17.599266604030554
8	25.866230077751272	35.16614461142788	20.815946028755167	18.15167928206568
9	26.511826499180753	15.50340116157367	20.644471513802216	37.340300825443364
10-11	25.13823636691056	25.921236455230428	28.489337383047864	20.45118979481115
12-13	25.78866855048866	23.49593717993468	28.437023475226464	22.278370794350195
14-15	22.839236856416434	24.69502171928535	25.982880640924304	26.482860783373912
16-17	22.565969721608614	27.680557823681294	26.656453051517655	23.097019403192434
18-19	22.55191889172789	27.083992563383223	28.22272629972685	22.141362245162032
20-21	23.423629774411598	26.464361746666988	27.78425377576969	22.327754703151722
22-23	23.270205595024382	26.310063259180243	27.659883521891572	22.759847623903802
24-25	22.910440451412466	26.460593193418664	27.824615663939767	22.804350691229107
26-27	22.775774650190662	26.718118143778902	27.758621567688955	22.74748563834148
28-29	22.67344469402297	26.889692392491916	27.72899260502486	22.707870308460254
30-31	23.059748853956954	26.66221277908562	27.50692444080399	22.77111392615344
32-33	22.8418987336933	26.900915289371103	27.481777415456747	22.775408561478848
34-35	22.816905426326056	26.969055827172657	27.58371672107295	22.63032202542834
36-37	22.930942559045523	26.766204451210264	27.54485904811434	22.757993941629877
38-39	22.82093634630887	26.8629173254119	27.600868582106212	22.71527774617301
40-41	22.9636762795462	26.68953775010663	27.698396608564956	22.64838936178222
42-43	22.914838561909292	26.82131220514857	27.349945683269166	22.913903549672973
44-45	22.916400755178547	27.226382371746983	27.029580145168016	22.827636727906462
46-47	23.04646663882086	26.94469340196011	26.900191155982593	23.10864880323644
48-49	23.07524917131535	26.876089492205736	26.879469505381586	23.16919183109733
50-51	22.826580579346306	27.037889471606174	26.87059217797683	23.264937771070684
52-53	23.02235805063424	27.118747300123484	26.619872375890534	23.239022273351736
54-55	23.08895913416353	26.90038383738793	26.670923065392632	23.339733963055902
56-57	23.19533057206169	26.932340735244324	26.74243336556844	23.129895327125542
58-59	23.113704341182384	27.049496615442404	26.84674410295208	22.990054940423136
60-61	23.206923489402627	27.1035218652099	26.546532567325205	23.143022078062263
62-63	23.190613023356182	27.074059425724066	26.57322321299949	23.162104337920262
64-65	23.02362493288081	27.056977639187668	26.609652504660065	23.309744923271456
66-67	23.096824505282846	27.099941422210865	26.419564361700115	23.383669710806174
68-69	23.08293415622145	27.043205288022175	26.604175853799607	23.269684701956766
70-71	23.144390459363958	26.73475265017668	26.589849823321554	23.53100706713781
72-73	23.176833108404004	26.89385811394745	26.631647368559513	23.29766140908903
74-75	22.904636091413245	27.146367553981836	26.673860289778244	23.275136064826672
76-77	23.073386607532214	27.003745885097764	26.516281004203517	23.40658650316651
78-79	23.18319166843382	27.03798354415177	26.405690783463605	23.373134003950806
80-81	23.050129887149946	27.08574510183469	26.525128964495504	23.33899604651986
82-83	23.399025193742766	26.86770967875265	26.699909212683725	23.03335591482086
84-85	23.419440284668873	26.89030974035425	26.42144165114911	23.26880832382777
86-87	22.953689181895022	27.155190362595093	26.647941507900125	23.24317894760976
88-89	23.25317884463462	26.872026018215173	26.62491416347169	23.24988097367852
90-91	23.25715049559807	26.89213781178766	26.620164738321563	23.230546954292716
92-93	23.420824495368144	26.901960487153215	26.54884465258477	23.128370364893872
94-95	23.074944928252965	27.056054384982808	26.580887223614862	23.288113463149358
96-97	22.967480342679053	26.98444363625802	26.76395431553706	23.284121705525866
98-99	23.38644922551525	27.54441715493674	26.721561894025324	22.347571725522688
100-101	27.916555012050082	34.72019137673087	27.00897171770611	10.354281893512946
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1123.0
1	1150.0
2	1931.5
3	2877.0
4	4138.5
5	6989.0
6	9186.0
7	10403.5
8	10865.5
9	9989.0
10	8383.0
11	7129.0
12	8018.5
13	11168.0
14	17713.0
15	26033.0
16	32093.5
17	34983.0
18	34682.5
19	31885.5
20	28471.5
21	25626.5
22	24595.0
23	26901.5
24	32319.0
25	40394.0
26	51788.0
27	64691.5
28	76123.5
29	88578.0
30	100164.5
31	110382.0
32	124653.0
33	140523.5
34	156160.5
35	172872.5
36	191240.5
37	206504.5
38	213254.0
39	216049.5
40	221889.0
41	229529.0
42	236403.0
43	245740.5
44	258068.0
45	274264.5
46	286177.0
47	293295.0
48	303092.0
49	310114.5
50	314378.0
51	317524.0
52	312169.5
53	305847.0
54	299424.0
55	284138.5
56	266648.0
57	249682.5
58	229655.0
59	201374.5
60	166875.0
61	133760.0
62	105912.0
63	83058.0
64	63657.5
65	47547.0
66	36239.5
67	28044.5
68	20967.5
69	15672.5
70	11891.5
71	8793.0
72	6499.5
73	4716.5
74	3365.5
75	2394.5
76	1569.0
77	997.5
78	677.0
79	475.5
80	319.0
81	212.5
82	161.5
83	118.0
84	83.5
85	59.5
86	44.5
87	34.0
88	21.5
89	10.5
90	7.0
91	5.5
92	3.0
93	2.5
94	3.0
95	2.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0013620702435395934
3	3.871147007954633E-4
4	0.0
5	0.0
6	4.731401898611219E-4
7	0.0
8	0.0
9	1.4337581510943087E-5
10-11	4.1578986381734953E-4
12-13	3.7994591003999185E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	8.607197008195701E-5
28-29	7.177984146417095E-6
30-31	2.1574262422712003E-5
32-33	2.308180299176232E-4
34-35	3.620165567548199E-5
36-37	8.225874656160258E-4
38-39	1.9819206264278547E-4
40-41	6.668814024782646E-5
42-43	0.0
44-45	7.5614635454659085E-6
46-47	0.0
48-49	0.0
50-51	2.35342662839472E-5
52-53	0.0
54-55	6.9737119914661E-4
56-57	1.0542288850929441E-4
58-59	0.0
60-61	9.223655702039026E-4
62-63	8.409641014290166E-6
64-65	8.506867040815693E-6
66-67	0.0
68-69	0.0
70-71	0.0
72-73	6.263775272208015E-5
74-75	2.7175365350140993E-5
76-77	0.0
78-79	5.573149679474229E-5
80-81	0.0
82-83	0.0
84-85	9.670772102839763E-6
86-87	3.8211797608372574E-4
88-89	9.933336386178717E-5
90-91	0.0
92-93	2.0479976931353984E-5
94-95	1.040709430804791E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	114.0
20-21	455.0
22-23	917.0
24-25	1648.0
26-27	3730.0
28-29	10288.0
30-31	20024.0
32-33	24580.0
34-35	32347.0
36-37	52636.0
38-39	64750.0
40-41	62493.0
42-43	68789.0
44-45	80390.0
46-47	82129.0
48-49	77721.0
50-51	70547.0
52-53	66024.0
54-55	70922.0
56-57	74536.0
58-59	73338.0
60-61	73546.0
62-63	68081.0
64-65	68815.0
66-67	72593.0
68-69	74588.0
70-71	73937.0
72-73	68612.0
74-75	67411.0
76-77	68022.0
78-79	71631.0
80-81	73708.0
82-83	68514.0
84-85	66748.0
86-87	68576.0
88-89	72537.0
90-91	76944.0
92-93	78084.0
94-95	82180.0
96-97	125654.0
98-99	551033.0
100-101	4065085.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.35772082051977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.72152402387891	71.3682326148526
2	7.929138517631755	12.901932757294288
3	2.3089714229695786	5.635579572375518
4	0.9295044498475813	3.024894541285214
5	0.42864537469472885	1.7436805362710421
6	0.22719518868968286	1.1090449639908317
7	0.1474997394156259	0.8400169834333137
8	0.08531378782221215	0.5552748265424476
9	0.053085327346906894	0.388701111776
>10	0.16541504531984605	1.997071689706383
>50	0.0026218649381674303	0.14604380657578256
>100	8.977342595646329E-4	0.12994403743740093
>500	1.2501545916617312E-4	0.07623144057600224
>1k	6.250772626953529E-5	0.08335111788315246
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4337581510943087E-5
2	0.0	0.0	0.0	0.0	1.4337581510943087E-5
3	0.0	0.0	0.0	0.0	1.4337581510943087E-5
4	0.0	0.0	0.0	0.0	1.4337581510943087E-5
5	0.0	0.0	0.0	0.0	1.4337581510943087E-5
6	0.0	0.0	0.0	0.0	4.301274453282926E-5
7	0.0	0.0	0.0	0.0	4.301274453282926E-5
8	0.0	0.0	0.0	0.0	4.301274453282926E-5
9	0.0	0.0	0.0	1.4337581510943087E-5	7.168790755471544E-5
10-11	0.0	0.0	0.0	3.584395377735772E-5	7.168790755471544E-5
12-13	0.0	0.0	0.0	5.0181535288300806E-5	7.168790755471544E-5
14-15	0.0	0.0	0.0	6.45191167992439E-5	1.147006520875447E-4
16-17	0.0	0.0	0.0	7.168790755471544E-5	1.2186944284301625E-4
18-19	0.0	0.0	0.0	1.5771339662037397E-4	1.4337581510943088E-4
20-21	0.0	0.0	0.0	2.580764671969756E-4	1.5054460586490242E-4
22-23	0.0	0.0	0.0	3.0108921172980485E-4	2.1506372266414634E-4
24-25	0.0	0.0	0.0	3.29764374751691E-4	2.580764671969756E-4
26-27	0.0	0.0	0.0	4.1578986381734953E-4	2.580764671969756E-4
28-29	0.0	0.0	0.0	9.176052167003577E-4	2.6524525795244716E-4
30-31	0.0	0.0	0.0	0.002623777416502585	2.795828394633902E-4
32-33	0.0	0.0	0.0	0.004953634412030837	2.8675163021886176E-4
34-35	0.0	0.0	0.0	0.0077566315974202105	2.8675163021886176E-4
36-37	0.0	0.0	0.0	0.01198621814314842	2.8675163021886176E-4
38-39	0.0	0.0	0.0	0.018696206290269787	2.8675163021886176E-4
40-41	0.0	0.0	0.0	0.02739194947665677	2.8675163021886176E-4
42-43	0.0	0.0	0.0	0.03501954284047849	2.9392042097433333E-4
44-45	0.0	0.0	0.0	0.04259695466901191	3.0825800248527636E-4
46-47	0.0	0.0	0.0	0.050948595899136265	3.29764374751691E-4
48-49	7.1687907554715436E-6	0.0	0.0	0.0596801830393006	3.441019562626341E-4
50-51	1.4337581510943087E-5	0.0	0.0	0.06889924795083702	3.441019562626341E-4
52-53	1.4337581510943087E-5	0.0	0.0	0.07699281271376437	3.584395377735772E-4
54-55	1.4337581510943087E-5	0.0	0.0	0.08494300166158232	3.7994591003999185E-4
56-57	1.4337581510943087E-5	0.0	0.0	0.09282867149260102	3.942834915509349E-4
58-59	1.4337581510943087E-5	0.0	0.0	0.1007430164866416	4.0145228230640645E-4
60-61	1.4337581510943087E-5	0.0	0.0	0.10948177241756141	4.0145228230640645E-4
62-63	1.4337581510943087E-5	0.0	0.0	0.11708069061836125	4.0145228230640645E-4
64-65	1.4337581510943087E-5	0.0	0.0	0.1240989367679679	4.0145228230640645E-4
66-67	1.4337581510943087E-5	0.0	0.0	0.13104549501001983	4.08621073061878E-4
68-69	1.4337581510943087E-5	0.0	0.0	0.13830748004531251	4.1578986381734953E-4
70-71	1.4337581510943087E-5	0.0	0.0	0.14473071656221498	4.1578986381734953E-4
72-73	1.4337581510943087E-5	0.0	0.0	0.15016465995486245	4.444650268392357E-4
74-75	1.4337581510943087E-5	0.0	0.0	0.15407165091659442	4.444650268392357E-4
76-77	1.4337581510943087E-5	7.1687907554715436E-6	0.0	0.1575986959682864	4.516338175947072E-4
78-79	1.4337581510943087E-5	1.4337581510943087E-5	0.0	0.1611544161830003	4.588026083501788E-4
80-81	1.4337581510943087E-5	1.4337581510943087E-5	0.0	0.16303980815168934	4.6597139910565036E-4
82-83	1.4337581510943087E-5	1.4337581510943087E-5	0.0	0.16374234964572554	5.01815352883008E-4
84-85	1.4337581510943087E-5	1.4337581510943087E-5	0.0	0.16415097071878743	5.01815352883008E-4
86-87	1.4337581510943087E-5	1.4337581510943087E-5	0.0	0.16431585290616324	5.01815352883008E-4
88-89	1.4337581510943087E-5	1.4337581510943087E-5	0.0	0.16440904718598437	5.161529343939512E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8265	0.0	44.250412	1
GTATCAA	14015	0.0	34.705357	1
ATCAACG	16915	0.0	27.97022	3
CAACGCA	17385	0.0	27.214443	5
TCAACGC	17720	0.0	26.77015	4
TATCAAC	18195	0.0	26.34495	2
AACGCAG	18380	0.0	25.786495	6
ACGCAGA	20240	0.0	23.396133	7
TACATGG	22535	0.0	22.908855	2
GTACATG	23255	0.0	22.770164	1
ACATGGG	22450	0.0	22.348648	3
CGCAGAG	21295	0.0	22.237038	8
CATGGGG	17805	0.0	19.888607	4
GCAGAGT	24980	0.0	18.956675	9
GTACTAG	1925	0.0	18.740454	1
AGAGTAC	22580	0.0	17.656748	10-11
GAGTACT	13520	0.0	17.374165	12-13
CAGAGTA	24190	0.0	17.184381	10-11
GAGTACA	16220	0.0	16.386974	1
CTAGTAC	2685	0.0	16.215384	3
>>END_MODULE