FastQCFastQC Report
Fri 27 May 2016
SRR2140104_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140104_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6869993
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80750.045.66511
GTATCAA142500.034.718651
ATCAACG168900.028.5952783
TCAACGC172200.028.047084
CAACGCA172550.027.9658495
TATCAAC179750.027.3135412
AACGCAG182250.026.8232616
ACGCAGA201450.023.9330237
TACATGG213500.023.133532
ACATGGG210300.023.0857873
CGCAGAG209800.022.9804948
GTACATG220950.022.7336731
GCAGAGT243900.019.733129
CATGGGG154950.019.6503094
GAGTACT134500.018.68801912-13
CAGAGTA237400.017.64635810-11
AGAGTAC221500.017.47207810-11
AGTACTT145250.016.45195412-13
GTACTTT158100.015.97827414-15
GAGTACA153000.015.8935011
Produced by FastQC (version 0.11.5)