FastQCFastQC Report
Fri 27 May 2016
SRR2140103_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140103_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5594631
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67550.038.6047021
GTATCAA114000.033.98361
ATCAACG130800.028.885713
CAACGCA131500.028.7319455
TCAACGC133000.028.5018634
AACGCAG136450.027.873066
TATCAAC141100.027.1613962
ACGCAGA152600.024.8412957
CGCAGAG155400.024.2864828
TACATGG153200.022.0794662
GTACATG160200.021.8947941
ACATGGG154350.021.2668463
CATGGGG103450.020.4155464
GCAGAGT187650.020.0237489
GAGTACT105900.018.56656312-13
AGAGTAC168150.017.81227710-11
CAGAGTA186450.016.9353510-11
TATACCG4301.8189894E-1216.4688765
CTGTGCG16300.016.35619
GGCGTCG6900.016.3005438
Produced by FastQC (version 0.11.5)