##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140103_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5594631
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79724346431427	34.0	31.0	34.0	31.0	34.0
2	32.99375812274304	34.0	33.0	34.0	31.0	34.0
3	33.102993745253265	34.0	33.0	34.0	31.0	34.0
4	36.4686684430126	37.0	37.0	37.0	35.0	37.0
5	36.34152815440375	37.0	37.0	37.0	35.0	37.0
6	36.369557706308065	37.0	37.0	37.0	35.0	37.0
7	36.37461612749795	37.0	37.0	37.0	35.0	37.0
8	36.38476514358141	37.0	37.0	37.0	35.0	37.0
9	38.28602190207004	39.0	39.0	39.0	37.0	39.0
10-11	38.19769176912651	39.0	39.0	39.0	37.0	39.0
12-13	38.18269533772647	39.0	39.0	39.0	37.0	39.0
14-15	39.69875913174613	41.0	40.0	41.0	37.5	41.0
16-17	39.67891197828776	41.0	40.0	41.0	37.5	41.0
18-19	39.71852862145868	41.0	40.0	41.0	38.0	41.0
20-21	39.738747067942235	41.0	40.0	41.0	38.0	41.0
22-23	39.691252160573214	41.0	40.0	41.0	37.5	41.0
24-25	39.676297850413164	41.0	40.0	41.0	37.0	41.0
26-27	39.56790707891105	41.0	39.5	41.0	37.0	41.0
28-29	39.504244374675636	41.0	39.0	41.0	37.0	41.0
30-31	39.438208555031665	40.5	39.0	41.0	37.0	41.0
32-33	39.357077619752204	40.0	39.0	41.0	36.0	41.0
34-35	39.32368323374566	40.0	39.0	41.0	36.0	41.0
36-37	39.28857481364595	40.0	39.0	41.0	36.0	41.0
38-39	39.231170375073035	40.0	39.0	41.0	36.0	41.0
40-41	39.174462100685616	40.0	39.0	41.0	35.0	41.0
42-43	39.17138874000172	40.0	39.0	41.0	35.0	41.0
44-45	39.12038306520708	40.0	39.0	41.0	35.0	41.0
46-47	39.02003714208564	40.0	39.0	41.0	35.0	41.0
48-49	39.02437486274542	40.0	39.0	41.0	35.0	41.0
50-51	38.93505889728184	40.0	38.5	41.0	35.0	41.0
52-53	38.79055120770333	40.0	38.0	41.0	35.0	41.0
54-55	38.61512769900102	40.0	38.0	41.0	35.0	41.0
56-57	38.49446079775134	40.0	38.0	41.0	35.0	41.0
58-59	38.36019648158548	40.0	37.0	41.0	34.0	41.0
60-61	38.15250466875182	40.0	37.0	41.0	34.0	41.0
62-63	38.016438416326366	39.5	36.5	41.0	34.0	41.0
64-65	37.76942173224894	39.0	36.0	41.0	34.0	41.0
66-67	37.554160930425525	39.0	35.5	41.0	34.0	41.0
68-69	37.305674271527536	38.5	35.0	40.0	33.5	41.0
70-71	36.86854052389694	37.5	35.0	40.0	33.0	41.0
72-73	36.60680596851334	37.0	35.0	39.5	33.0	41.0
74-75	36.23963659712513	36.5	35.0	39.0	32.5	41.0
76-77	34.40025430070883	34.5	33.0	37.0	30.0	39.0
78-79	35.407387913214954	35.0	34.5	37.5	32.0	39.0
80-81	35.315259712327865	35.0	35.0	37.0	32.0	39.0
82-83	35.015368664483546	35.0	34.5	37.0	32.0	39.0
84-85	34.74751771111151	35.0	34.0	36.0	32.0	37.5
86-87	34.53376369842324	35.0	34.0	36.0	32.0	37.0
88-89	34.34351628454251	35.0	34.0	36.0	32.0	37.0
90-91	34.25391375646089	35.0	34.0	35.0	32.0	36.5
92-93	34.115446594383975	35.0	34.0	35.0	31.5	36.0
94-95	33.95391764030348	35.0	34.0	35.0	31.0	36.0
96-97	33.971063742225624	35.0	34.0	35.0	31.0	36.0
98-99	33.989370351722926	35.0	34.0	35.0	31.0	35.5
100-101	32.7912954967705	34.5	32.0	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	10.0
20	16.0
21	55.0
22	220.0
23	666.0
24	1784.0
25	3889.0
26	7583.0
27	12977.0
28	20140.0
29	28809.0
30	39765.0
31	54223.0
32	73802.0
33	104612.0
34	165612.0
35	275024.0
36	563480.0
37	1284210.0
38	1932893.0
39	1000846.0
40	24010.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.17430961934755	20.414286482879746	13.194328634006425	24.21707526376628
2	14.539412946003653	21.324343624012318	41.56052011378941	22.575723316194615
3	17.937992416266667	26.80321009200648	30.514642139231967	24.744155352494886
4	11.876636725460536	18.027819886601996	40.10602307819765	29.98952030973982
5	12.563491676216001	38.430202099119676	36.30604055924332	12.700265665421009
6	29.19288986061922	38.83652492170131	19.29486673046626	12.675718487213212
7	25.87831083050875	32.84733166494805	24.155963101051707	17.11839440349149
8	24.110776921659355	36.90674148125229	21.81117932532101	17.171302271767342
9	25.467108805089456	16.427186937183315	21.735404034012628	36.37030022371461
10-11	23.8406174793766	26.929506868746667	29.619307205356005	19.610568446520727
12-13	24.626142227135684	24.519539574492043	29.586338273060264	21.26797992531201
14-15	21.67679155247236	25.92753659714108	27.218077474635948	25.177594375750605
16-17	21.32058396702124	28.91795723435558	27.869523477062202	21.891935321560975
18-19	21.193998317315295	28.410658361561293	29.430457522578347	20.96488579854507
20-21	22.160481689954544	27.707289519829402	29.079912842503404	21.052315947712653
22-23	21.851804976289806	27.68552130878635	28.853968184165662	21.608705530758186
24-25	21.51847552967166	27.72409336939152	29.12900027211702	21.628430828819802
26-27	21.659748640426542	27.993849292097195	28.814408666360315	21.53199340111595
28-29	21.441309463325698	28.22906624465785	28.839056407290283	21.49056788472617
30-31	21.788248424273245	27.973077887625376	28.79340945484452	21.445264233256857
32-33	21.503897620435637	28.1058549032528	28.76357132872382	21.626676147587737
34-35	21.57213212810019	28.2046499809748	28.823336689023193	21.399881201901817
36-37	21.680690869851375	28.08049927403146	28.69874052056548	21.540069335551685
38-39	21.46758994840493	28.19406293198807	28.8579807274159	21.480366392191097
40-41	21.7301390024077	28.00185711905638	28.762712889116976	21.505290989418942
42-43	21.695690068597155	28.12215055750792	28.559606936700504	21.62255243719442
44-45	21.766839118232745	28.394357010424017	28.321655640480518	21.51714823086272
46-47	21.74044315643025	28.237998871208557	28.103828608210822	21.917729364150368
48-49	21.84995093204993	28.249617221765433	28.14756553042816	21.752866315756478
50-51	21.658709668986166	28.37414452763996	28.036774459256797	21.93037134411708
52-53	21.79194100803052	28.342922958872652	27.89346840825623	21.9716676248406
54-55	21.8738371041373	28.31875040340285	27.876620882601603	21.93079160985825
56-57	21.928316326967405	28.349091109797946	27.860197328166276	21.86239523506837
58-59	21.853563044275166	28.453147481263088	27.95963329223347	21.733656182228273
60-61	21.903103969128825	28.429401206214433	27.833127453360067	21.834367371296672
62-63	21.865013033575856	28.350927713971565	27.83133576198707	21.952723490465516
64-65	21.79610230067247	28.396051534506928	27.852596465987883	21.955249698832727
66-67	21.893050524203208	28.36684505291481	27.770983261475816	21.969121161406164
68-69	21.91319092285253	28.33470158638264	27.83520653862464	21.916900952140193
70-71	21.868732141550833	28.11676818982337	27.85784865534135	22.15665101328445
72-73	21.967838003547914	28.202805444145962	27.97925932278389	21.850097229522234
74-75	21.896730788364856	28.290145187742382	27.81761146208428	21.995512561808482
76-77	21.803245326654277	28.286523047072652	27.7868269507158	22.123404675557268
78-79	21.889370452189276	28.253154093323037	27.780430796567778	22.07704465791991
80-81	21.78931671948359	28.27468973681983	27.839329880231396	22.096663663465186
82-83	21.92786582864185	28.200542904960855	27.993562003653118	21.878029262744178
84-85	22.160230186006626	28.11718837033258	27.810789689662403	21.911791753998394
86-87	21.791824008081047	28.242793911675673	27.869543886037228	22.09583819420605
88-89	21.98999859896006	28.167706797579967	27.860270592147273	21.982024011312696
90-91	22.063745145024637	28.156563830262016	27.900063136159858	21.87962788855349
92-93	22.006319129194946	28.157579599666395	27.90782736376863	21.92827390737003
94-95	21.824733587816485	28.323654086425552	27.893305003291918	21.95830732246604
96-97	21.81411709824254	28.268762616670408	27.997556411499367	21.91956387358768
98-99	22.145688371408824	28.864989692715987	27.9723365796744	21.01698535620079
100-101	26.058371389296596	35.869851451110485	28.627655488810678	9.444121670782247
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	664.0
1	676.5
2	1256.5
3	1931.5
4	2866.0
5	5043.0
6	6694.5
7	7449.5
8	7501.5
9	6764.0
10	5827.5
11	5096.0
12	5790.0
13	8212.5
14	13326.5
15	19938.5
16	24963.5
17	27926.0
18	28696.5
19	27498.5
20	25724.0
21	24449.5
22	25123.5
23	28688.0
24	34858.5
25	43899.5
26	55920.5
27	69128.0
28	82440.5
29	96890.5
30	110083.5
31	122150.5
32	135959.0
33	149728.0
34	163036.0
35	176582.5
36	189609.5
37	201499.5
38	208952.0
39	213027.5
40	220013.0
41	225434.0
42	227756.0
43	232628.0
44	237949.5
45	242854.5
46	245868.0
47	247664.5
48	246705.0
49	240525.5
50	232756.5
51	223613.5
52	211741.0
53	200060.0
54	186810.5
55	169655.5
56	152787.5
57	134834.5
58	116513.5
59	99788.5
60	82900.0
61	65903.5
62	50718.5
63	38379.0
64	28448.0
65	20800.0
66	15579.5
67	12009.5
68	9100.5
69	6763.5
70	5059.0
71	3878.0
72	2933.5
73	2150.5
74	1509.5
75	995.0
76	663.5
77	419.5
78	262.5
79	168.0
80	113.5
81	87.5
82	75.5
83	56.0
84	40.5
85	26.5
86	15.5
87	11.0
88	6.0
89	3.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0015371880647713854
3	4.4685699557307714E-4
4	0.0
5	0.0
6	5.183541148647694E-4
7	0.0
8	0.0
9	5.3622839468769254E-5
10-11	2.6811419734384625E-4
12-13	4.200455758386925E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	6.259439458254457E-5
28-29	8.947941324769557E-6
30-31	4.481459708226499E-5
32-33	2.4271578467046658E-4
34-35	9.023096419906034E-6
36-37	5.443033438822345E-4
38-39	6.401936238178368E-5
40-41	4.615104869027939E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	6.828361619496182E-5
52-53	0.0
54-55	5.17946652291655E-4
56-57	3.0215922985655493E-5
58-59	0.0
60-61	7.9362363604956E-4
62-63	1.0423107278372427E-5
64-65	0.0
66-67	2.13142675788889E-5
68-69	0.0
70-71	0.0
72-73	7.735918721575333E-5
74-75	2.2358929686979458E-5
76-77	1.1312250465895035E-5
78-79	4.579651532025102E-5
80-81	0.0
82-83	0.0
84-85	1.1889280363260317E-5
86-87	4.8147083562633815E-4
88-89	9.754837759367419E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	96.0
20-21	363.0
22-23	736.0
24-25	1371.0
26-27	2840.0
28-29	6893.0
30-31	15799.0
32-33	19408.0
34-35	26166.0
36-37	41071.0
38-39	50750.0
40-41	49055.0
42-43	54036.0
44-45	63390.0
46-47	63303.0
48-49	59737.0
50-51	54472.0
52-53	51713.0
54-55	54963.0
56-57	56957.0
58-59	55955.0
60-61	55303.0
62-63	51897.0
64-65	52895.0
66-67	55520.0
68-69	56379.0
70-71	56158.0
72-73	52099.0
74-75	52394.0
76-77	52224.0
78-79	54725.0
80-81	55848.0
82-83	52047.0
84-85	51004.0
86-87	52886.0
88-89	55311.0
90-91	58213.0
92-93	58668.0
94-95	62673.0
96-97	96240.0
98-99	435570.0
100-101	3347503.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.63757937068273
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.61946368456593	74.11917427705218
2	7.634688132879348	12.770936693682122
3	2.1089119670713083	5.291528760951275
4	0.8034979310402904	2.688104879262466
5	0.35654334908072904	1.4910211328914256
6	0.1873770130930579	0.9403055882887251
7	0.09725838890857877	0.5694119355264191
8	0.05824417826458865	0.38971216659877816
9	0.035736514035108316	0.26900239761386
>10	0.0963079642226842	1.121066387486111
>50	8.35201139279752E-4	0.04794266929446639
>100	8.979273394804525E-4	0.16089583746235148
>500	1.6982027361865995E-4	0.07272118567309795
>1k	6.792808600486071E-5	0.06817608821676065
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	3.574855964584617E-5	0.0	0.0	0.0
5	0.0	3.574855964584617E-5	0.0	0.0	0.0
6	0.0	3.574855964584617E-5	0.0	0.0	0.0
7	0.0	3.574855964584617E-5	0.0	1.0724567893753851E-4	0.0
8	0.0	3.574855964584617E-5	0.0	1.6086851840630775E-4	0.0
9	0.0	1.251199587604616E-4	0.0	3.0386275698969246E-4	0.0
10-11	0.0	1.4299423858338468E-4	0.0	3.574855964584617E-4	0.0
12-13	0.0	1.4299423858338468E-4	0.0	3.8429701619284635E-4	0.0
14-15	0.0	1.6086851840630775E-4	0.0	4.647312753960002E-4	0.0
16-17	0.0	1.6086851840630775E-4	0.0	5.183541148647694E-4	0.0
18-19	0.0	1.6086851840630775E-4	0.0	5.362283946876925E-4	0.0
20-21	0.0	1.7874279822923086E-4	1.7874279822923085E-5	6.43474073625231E-4	0.0
22-23	0.0	1.7874279822923086E-4	1.7874279822923085E-5	6.43474073625231E-4	0.0
24-25	0.0	2.2342849778653857E-4	1.7874279822923085E-5	6.702854933596157E-4	0.0
26-27	0.0	2.323656376980001E-4	1.7874279822923085E-5	7.41782612651308E-4	0.0
28-29	0.0	2.323656376980001E-4	1.7874279822923085E-5	0.0010009596700836928	0.0
30-31	0.0	2.502399175209232E-4	1.7874279822923085E-5	0.0022164106980424626	0.0
32-33	0.0	2.502399175209232E-4	1.7874279822923085E-5	0.004432821396084925	0.0
34-35	0.0	2.8598847716676935E-4	1.7874279822923085E-5	0.007113963369523388	0.0
36-37	0.0	3.217370368126155E-4	1.7874279822923085E-5	0.011305481987998852	0.0
38-39	0.0	3.396113166355386E-4	1.7874279822923085E-5	0.017632977045313622	0.0
40-41	0.0	3.396113166355386E-4	1.7874279822923085E-5	0.02490780893324332	0.0
42-43	0.0	3.396113166355386E-4	1.7874279822923085E-5	0.03169109812604263	0.0
44-45	0.0	3.5748559645846167E-4	1.7874279822923085E-5	0.038751438656097245	0.0
46-47	0.0	3.7535987628138477E-4	1.7874279822923085E-5	0.04644631611986563	0.0
48-49	1.7874279822923085E-5	3.7535987628138477E-4	1.7874279822923085E-5	0.054346747801597636	0.0
50-51	1.7874279822923085E-5	4.379198556616156E-4	1.7874279822923085E-5	0.062479545121027644	0.0
52-53	1.7874279822923085E-5	4.4685699557307714E-4	1.7874279822923085E-5	0.07008505118568142	0.0
54-55	1.7874279822923085E-5	4.5579413548453866E-4	1.7874279822923085E-5	0.07778886578936126	0.0
56-57	1.7874279822923085E-5	4.647312753960002E-4	1.7874279822923085E-5	0.08606465734737465	0.0
58-59	1.7874279822923085E-5	4.647312753960002E-4	1.7874279822923085E-5	0.09395615188919519	0.0
60-61	1.7874279822923085E-5	4.647312753960002E-4	1.7874279822923085E-5	0.10140972657535413	0.0
62-63	1.7874279822923085E-5	4.647312753960002E-4	1.7874279822923085E-5	0.1084790042453202	0.0
64-65	1.7874279822923085E-5	5.45165534599154E-4	1.7874279822923085E-5	0.11460988222458282	0.0
66-67	1.7874279822923085E-5	5.541026745106157E-4	1.7874279822923085E-5	0.12039221174729844	0.0
68-69	1.7874279822923085E-5	5.809140942450002E-4	1.7874279822923085E-5	0.12714868952036334	0.0
70-71	1.7874279822923085E-5	5.987883740679234E-4	1.7874279822923085E-5	0.1335566188368813	0.0
72-73	1.7874279822923085E-5	6.077255139793849E-4	1.7874279822923085E-5	0.1388742170842009	0.0
74-75	1.7874279822923085E-5	6.434740736252311E-4	1.7874279822923085E-5	0.14357515267762969	0.0
76-77	1.7874279822923085E-5	6.702854933596157E-4	1.7874279822923085E-5	0.1477041113167249	0.0
78-79	1.7874279822923085E-5	6.792226332710772E-4	1.7874279822923085E-5	0.15162751573785654	0.0
80-81	1.7874279822923085E-5	6.792226332710772E-4	1.7874279822923085E-5	0.1533523837407686	0.0
82-83	2.6811419734384627E-5	6.792226332710772E-4	1.7874279822923085E-5	0.15401373209421676	0.0
84-85	3.574855964584617E-5	6.792226332710772E-4	1.7874279822923085E-5	0.15453208620908154	0.0
86-87	3.574855964584617E-5	6.792226332710772E-4	1.7874279822923085E-5	0.15469295472748784	0.0
88-89	3.574855964584617E-5	7.149711929169234E-4	1.7874279822923085E-5	0.1547018918673993	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6755	0.0	38.604702	1
GTATCAA	11400	0.0	33.9836	1
ATCAACG	13080	0.0	28.88571	3
CAACGCA	13150	0.0	28.731945	5
TCAACGC	13300	0.0	28.501863	4
AACGCAG	13645	0.0	27.87306	6
TATCAAC	14110	0.0	27.161396	2
ACGCAGA	15260	0.0	24.841295	7
CGCAGAG	15540	0.0	24.286482	8
TACATGG	15320	0.0	22.079466	2
GTACATG	16020	0.0	21.894794	1
ACATGGG	15435	0.0	21.266846	3
CATGGGG	10345	0.0	20.415546	4
GCAGAGT	18765	0.0	20.023748	9
GAGTACT	10590	0.0	18.566563	12-13
AGAGTAC	16815	0.0	17.812277	10-11
CAGAGTA	18645	0.0	16.93535	10-11
TATACCG	430	1.8189894E-12	16.468876	5
CTGTGCG	1630	0.0	16.3561	9
GGCGTCG	690	0.0	16.300543	8
>>END_MODULE