FastQCFastQC Report
Fri 27 May 2016
SRR2140102_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140102_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6145512
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91150.038.394841
GTATCAA153400.032.7161181
ATCAACG182350.026.8550343
CAACGCA182700.026.8038065
TATCAAC193100.025.9060082
TCAACGC190600.025.9053544
AACGCAG192750.025.7006636
ACGCAGA218850.022.4133267
CGCAGAG219600.022.3183338
ACATGGG219600.022.0599253
TACATGG226450.021.9655382
GTACATG237700.021.4030781
GTACTAG21350.019.9208181
ACGGTAC6350.019.7739373
CATGGGG161050.019.6677534
GCAGAGT262850.018.6922479
CGACGAG9550.017.46040224-25
GAGTACT140300.017.33639312-13
CAATGCG9600.017.30520818-19
AGAGTAC236500.017.15247310-11
Produced by FastQC (version 0.11.5)