FastQCFastQC Report
Fri 27 May 2016
SRR2140101_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140101_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5477393
Sequences flagged as poor quality0
Sequence length19-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60500.040.0004921
CTTATAC85350.034.111381
GTATCAA105450.033.7059361
TATACAC94200.032.2107283
TTATACA101750.029.015772
ATCAACG123300.027.8964843
CAACGCA124600.027.6059325
TCAACGC126500.027.223674
TATCAAC132900.026.4051762
AACGCAG130700.026.3177576
TACACAT125500.024.145115
ACGCAGA146300.023.483077
ATACACA139150.022.7476984
GTACATG169600.022.5021131
TACATGG167200.022.4332642
ACACATC130600.022.2304486
ACATGGG165700.022.1915233
CGCAGAG155700.022.0127378
CACATCT148600.019.5097487
CATGGGG125500.019.1203164
Produced by FastQC (version 0.11.5)