##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140101_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5477393
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74671618414089	34.0	31.0	34.0	31.0	34.0
2	32.95223128959343	34.0	33.0	34.0	31.0	34.0
3	33.06046361836735	34.0	33.0	34.0	31.0	34.0
4	36.43992132753666	37.0	37.0	37.0	35.0	37.0
5	36.303482149263345	37.0	37.0	37.0	35.0	37.0
6	36.32882504505337	37.0	37.0	37.0	35.0	37.0
7	36.331409303659605	37.0	37.0	37.0	35.0	37.0
8	36.34065950717796	37.0	37.0	37.0	35.0	37.0
9	38.24414297093526	39.0	39.0	39.0	37.0	39.0
10-11	38.14847218375603	39.0	38.5	39.0	37.0	39.0
12-13	38.13371178588062	39.0	38.5	39.0	37.0	39.0
14-15	39.629091065767966	41.0	39.5	41.0	37.0	41.0
16-17	39.59958953100499	41.0	39.0	41.0	37.0	41.0
18-19	39.638914899843776	41.0	39.5	41.0	37.0	41.0
20-21	39.657070848914316	41.0	40.0	41.0	37.0	41.0
22-23	39.60318068867349	41.0	39.5	41.0	37.0	41.0
24-25	39.58060545224446	41.0	39.0	41.0	37.0	41.0
26-27	39.46280743937045	41.0	39.0	41.0	37.0	41.0
28-29	39.40068591486819	40.5	39.0	41.0	37.0	41.0
30-31	39.34866109092417	40.0	39.0	41.0	36.0	41.0
32-33	39.25805915835074	40.0	39.0	41.0	36.0	41.0
34-35	39.20674422873871	40.0	39.0	41.0	36.0	41.0
36-37	39.171576335124996	40.0	39.0	41.0	35.5	41.0
38-39	39.09502883003981	40.0	39.0	41.0	35.0	41.0
40-41	39.04476925339195	40.0	39.0	41.0	35.0	41.0
42-43	39.03812679498178	40.0	39.0	41.0	35.0	41.0
44-45	38.97377943183921	40.0	38.0	41.0	35.0	41.0
46-47	38.86382668040552	40.0	38.0	41.0	35.0	41.0
48-49	38.8545648897929	40.0	38.0	41.0	35.0	41.0
50-51	38.714662258993044	40.0	38.0	41.0	35.0	41.0
52-53	38.551961912941564	40.0	38.0	41.0	35.0	41.0
54-55	38.337908261848625	40.0	37.5	41.0	34.0	41.0
56-57	38.16124503491706	40.0	37.0	41.0	34.0	41.0
58-59	37.986062337400185	39.5	36.5	41.0	34.0	41.0
60-61	37.71687087978381	39.0	36.0	41.0	33.5	41.0
62-63	37.53854389708526	39.0	35.5	41.0	33.0	41.0
64-65	37.27124607494241	38.5	35.0	40.0	33.0	41.0
66-67	37.031872246464474	38.0	35.0	40.0	33.0	41.0
68-69	36.767835547215896	37.0	35.0	40.0	33.0	41.0
70-71	36.316234341501264	37.0	35.0	39.0	32.5	41.0
72-73	36.05273210456231	36.0	35.0	39.0	32.0	41.0
74-75	35.69752520965863	36.0	35.0	38.5	32.0	40.0
76-77	33.93460284484899	34.5	32.0	36.5	29.0	39.0
78-79	34.942637922975024	35.0	34.0	37.0	31.5	39.0
80-81	34.88592208388806	35.0	34.0	36.5	32.0	39.0
82-83	34.63767492794533	35.0	34.0	36.0	32.0	37.5
84-85	34.41546844091444	35.0	34.0	36.0	31.0	37.0
86-87	34.247325589344655	35.0	34.0	35.5	31.0	37.0
88-89	34.09587210358727	35.0	34.0	35.0	31.0	36.0
90-91	34.048685051406295	35.0	34.0	35.0	31.0	36.0
92-93	33.94312957003682	35.0	34.0	35.0	31.0	36.0
94-95	33.80299997575235	35.0	34.0	35.0	31.0	36.0
96-97	33.837552585259346	35.0	34.0	35.0	31.0	35.0
98-99	33.87685645415857	35.0	34.0	35.0	31.0	35.0
100-101	32.65216730314023	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	7.0
20	25.0
21	61.0
22	234.0
23	816.0
24	2151.0
25	4800.0
26	9049.0
27	14755.0
28	22625.0
29	32368.0
30	43367.0
31	57830.0
32	78372.0
33	112020.0
34	179061.0
35	304589.0
36	634658.0
37	1387562.0
38	1737109.0
39	836022.0
40	19911.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.487950015637	19.83085749005047	12.747652030811008	24.933540463501526
2	15.41447558559215	20.575668497671945	40.348097308190376	23.66175860854553
3	18.360123891688794	26.019755849756226	30.17586010791685	25.444260150638126
4	12.48294581016918	17.504641350365034	37.81171079015145	32.20070204931433
5	13.199290246290527	37.878950807437036	35.59030728669643	13.331451659576008
6	30.70348879879679	37.338914126117004	18.255023681125095	13.702573393961103
7	27.064481223092812	31.98386531694914	22.66591058921644	18.28574287074161
8	25.41141378754455	35.620303308526516	21.090106187377827	17.878176716551103
9	25.918794930141935	15.80011070962239	20.34008155706219	37.94101280317348
10-11	24.753632121062306	26.060954712272917	28.53889214876169	20.64652101790309
12-13	25.49749866564769	23.346386723644066	28.42874686448887	22.727367746219382
14-15	22.71349709615505	24.759689509224554	25.54376689786546	26.983046496754937
16-17	22.708595129106126	28.14564337450316	26.13972559573505	23.006035900655657
18-19	22.69951234099872	27.107996450136042	27.65237951704397	22.540111691821274
20-21	23.208446947324795	26.40293614764357	27.431080771446233	22.957536133585407
22-23	22.949499497607817	26.663595528554374	27.3235706928703	23.063334280967506
24-25	22.851757475960042	26.838309180230485	27.58836366863708	22.721569675172397
26-27	22.72236059392973	26.992200950426394	27.566968709727213	22.718469745916664
28-29	22.25365447118765	26.672098403027466	27.64371441192786	23.430532713857026
30-31	22.63950432486827	26.526414264478444	27.691217125080943	23.142864285572347
32-33	22.44657661340327	26.880412266963067	27.714554732389317	22.95845638724434
34-35	22.45689489013479	27.34471674786215	27.856254757511913	22.342133604491146
36-37	22.74004737923355	26.912621456469505	27.275139461570614	23.072191702726332
38-39	22.773896780044776	26.710899718384546	27.58259689284626	22.932606608724416
40-41	22.739871196071835	26.575441099702218	27.862630177951353	22.82205752627459
42-43	23.04233001587974	26.482107672779243	27.69297855156833	22.78258375977268
44-45	22.627885228008797	27.15183014262336	27.354727560972197	22.865557068395646
46-47	23.182002075291805	26.567095907458516	26.88094935465381	23.369952662595868
48-49	23.063463828786187	26.540471522351762	27.06716286575252	23.328901783109533
50-51	22.60722967388423	26.85249214371619	27.23291063610487	23.307367546294717
52-53	22.732380391343384	27.042426594755074	27.32552580487283	22.899667209028706
54-55	22.780626324201915	26.945150435073696	26.94588795228755	23.328335288436836
56-57	23.253605929171226	27.098937366994413	26.6219181784834	23.02553852535096
58-59	22.674633753684777	27.397956649099864	27.274278263842174	22.65313133337319
60-61	23.211570884629378	27.867706594876402	26.259087663496807	22.661634856997416
62-63	22.80282796543598	28.032136955873987	26.344177479210117	22.820857599479915
64-65	22.677683393026708	27.78620164707278	26.494119853117652	23.04199510678286
66-67	22.879075356597376	27.50844788466848	26.428871290085134	23.18360546864901
68-69	22.94394132832967	27.32998446669439	26.582256451508695	23.14381775346724
70-71	23.00573426965715	26.944707392266455	26.60564225170649	23.443916086369903
72-73	23.06235144195197	27.02444927931651	26.639151887105477	23.27404739162604
74-75	22.8229406422648	27.145634339818674	26.723612464593533	23.307812553322993
76-77	23.038356548826478	27.10354358416101	26.474820903822767	23.38327896318975
78-79	23.05144112942594	27.0710864084025	26.486141341734015	23.391331120437545
80-81	22.972855104967195	27.112066375818667	26.527775498866664	23.387303020347474
82-83	23.244015592927045	26.971794351662275	26.66487633241101	23.119313722999667
84-85	23.29180545263175	26.956401749556075	26.444565155769823	23.30722764204236
86-87	22.933918216389504	27.195146612220196	26.638083296983833	23.232851874406467
88-89	23.173961528647773	26.90132766883549	26.633861637634247	23.290849164882495
90-91	23.206560863202917	26.848323922432684	26.63001591473451	23.31509929962989
92-93	23.272837759481707	26.96141653605464	26.59472547532445	23.17102022913921
94-95	23.013315293775367	27.048249072774745	26.682677321235015	23.255758312214876
96-97	22.899427271630795	27.006436474114686	26.791659853777528	23.30247640047699
98-99	23.332878504499618	27.564973892245543	26.75300302910209	22.349144574152742
100-101	27.8903034293443	34.65967979392855	27.108698652782547	10.34131812394461
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	610.0
1	628.5
2	1202.0
3	1853.0
4	2724.0
5	4757.5
6	6259.5
7	6957.0
8	7100.0
9	6422.0
10	5434.5
11	4693.0
12	5202.0
13	7187.5
14	11680.5
15	17436.5
16	21687.0
17	23806.0
18	23751.5
19	22219.5
20	20264.5
21	18683.5
22	18414.5
23	20461.5
24	24949.0
25	31672.0
26	40803.0
27	51088.5
28	60444.5
29	70390.5
30	80034.5
31	87745.5
32	97255.0
33	108140.5
34	119408.0
35	132184.0
36	146836.5
37	159585.5
38	167860.0
39	173389.5
40	179114.5
41	185776.0
42	192122.0
43	200774.0
44	212661.5
45	228483.0
46	245539.0
47	262194.0
48	270181.5
49	271096.0
50	273648.5
51	270546.5
52	259404.0
53	248373.5
54	236326.0
55	220235.5
56	205032.5
57	189365.5
58	169486.0
59	145799.5
60	120154.5
61	95698.0
62	75104.0
63	57829.5
64	43671.5
65	31937.0
66	23474.0
67	17861.5
68	13622.0
69	10233.0
70	7494.0
71	5511.5
72	4016.5
73	2822.5
74	2029.0
75	1442.5
76	929.0
77	585.5
78	403.5
79	282.0
80	199.5
81	147.0
82	107.5
83	67.0
84	43.0
85	28.0
86	21.0
87	17.0
88	10.0
89	4.5
90	3.0
91	3.0
92	3.0
93	2.5
94	1.5
95	1.0
96	0.5
97	0.0
98	1.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0013510076782878278
3	5.111920944872862E-4
4	0.0
5	0.0
6	6.389901181091078E-4
7	0.0
8	0.0
9	1.8256860517403078E-5
10-11	2.738529077610462E-4
12-13	3.0123819853715075E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.4800646209220104E-5
28-29	9.140233112505301E-6
30-31	5.4943932921516344E-5
32-33	1.9290529241181313E-4
34-35	1.8444433377784415E-5
36-37	5.379126891655759E-4
38-39	2.1518027616423754E-4
40-41	8.502026930453703E-5
42-43	0.0
44-45	9.646163296425827E-6
46-47	3.907901317285146E-5
48-49	2.967775889392971E-5
50-51	2.002940115795977E-5
52-53	0.0
54-55	7.579980574763294E-4
56-57	8.303921880024522E-5
58-59	0.0
60-61	9.181530603856456E-4
62-63	1.0835115438945153E-5
64-65	0.0
66-67	5.5959195450024963E-5
68-69	0.0
70-71	0.0
72-73	1.05297973961683E-4
74-75	2.3699102917857248E-5
76-77	2.400712243308345E-5
78-79	6.082276659409295E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	5.527921660865349E-4
88-89	1.3038202323955258E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	87.0
20-21	346.0
22-23	764.0
24-25	1317.0
26-27	2988.0
28-29	7882.0
30-31	16332.0
32-33	20182.0
34-35	26364.0
36-37	43090.0
38-39	52842.0
40-41	50756.0
42-43	55413.0
44-45	64412.0
46-47	64526.0
48-49	62700.0
50-51	57675.0
52-53	53554.0
54-55	61795.0
56-57	66979.0
58-59	66451.0
60-61	69645.0
62-63	70064.0
64-65	73389.0
66-67	75645.0
68-69	65186.0
70-71	59793.0
72-73	54510.0
74-75	53824.0
76-77	54400.0
78-79	57081.0
80-81	58226.0
82-83	53714.0
84-85	52758.0
86-87	53776.0
88-89	57158.0
90-91	59595.0
92-93	59891.0
94-95	63330.0
96-97	96412.0
98-99	422458.0
100-101	3090083.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.79651358428694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.3154427808607	73.1221075790785
2	7.775740761628719	12.876084511961716
3	2.0937979285474393	5.200775059011897
4	0.8436291679130653	2.793982154448592
5	0.3935334040455016	1.6291596916962021
6	0.2083464897097537	1.0350217779295272
7	0.11895478056104819	0.6894328773247088
8	0.07216491124783657	0.47800024435522886
9	0.04715689389225151	0.35139837651683214
>10	0.12868219919126533	1.540623136783854
>50	0.0014670268545422952	0.07706752584496411
>100	0.0010836555478069194	0.20634706504790798
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.8256860517403078E-5	0.0	0.0	0.0
5	0.0	1.8256860517403078E-5	0.0	0.0	0.0
6	0.0	1.8256860517403078E-5	0.0	0.0	0.0
7	0.0	1.8256860517403078E-5	0.0	0.0	0.0
8	0.0	1.8256860517403078E-5	0.0	0.0	0.0
9	0.0	1.2779802362182155E-4	0.0	0.0	0.0
10-11	0.0	1.2779802362182155E-4	0.0	9.128430258701539E-6	0.0
12-13	0.0	1.2779802362182155E-4	0.0	3.6513721034806156E-5	0.0
14-15	0.0	1.825686051740308E-4	0.0	4.564215129350769E-5	0.0
16-17	0.0	1.825686051740308E-4	0.0	1.0954116310441848E-4	0.0
18-19	0.0	1.9169703543273233E-4	0.0	1.369264538805231E-4	0.0
20-21	0.0	2.0082546569143386E-4	0.0	1.643117446566277E-4	0.0
22-23	0.0	2.1908232620883693E-4	0.0	2.099538959501354E-4	0.0
24-25	0.0	2.2821075646753847E-4	0.0	2.9210976827844925E-4	0.0
26-27	0.0	2.4646761698494154E-4	0.0	4.8380680371118163E-4	0.0
28-29	0.0	2.555960472436431E-4	0.0	0.0014422919808748432	0.0
30-31	0.0	3.103666287958523E-4	0.0	0.004454673966246351	0.0
32-33	0.0	3.286234893132554E-4	0.0	0.008589852873438148	0.0
34-35	0.0	4.290362221589723E-4	0.0	0.0142403512035744	0.0
36-37	0.0	5.294489550046892E-4	0.0	0.021753049306485767	0.0
38-39	0.0	5.477058155220923E-4	0.0	0.037663903247402555	0.0
40-41	0.0	5.842195365568985E-4	0.0	0.07446060562022846	0.0
42-43	0.0	5.842195365568985E-4	0.0	0.08868269996328546	0.0
44-45	0.0	6.116048273330031E-4	0.0	0.10731382612129531	0.0
46-47	0.0	6.389901181091078E-4	0.0	0.12767935402845842	0.0
48-49	0.0	6.389901181091078E-4	0.0	0.1469494703045774	0.0
50-51	0.0	7.1201756017872E-4	0.0	0.16737889722355143	0.0
52-53	0.0	7.1201756017872E-4	0.0	0.19293850194791573	0.0
54-55	0.0	7.1201756017872E-4	0.0	0.24034244028135282	0.0
56-57	0.0	7.1201756017872E-4	0.0	0.29167160362603156	0.0
58-59	0.0	7.1201756017872E-4	0.0	0.34311943656407345	0.0
60-61	0.0	7.1201756017872E-4	0.0	0.37644733543859277	0.0
62-63	0.0	7.394028509548247E-4	0.0	0.39247685897287266	0.0
64-65	0.0	7.485312812135262E-4	0.0	0.4074748698879193	0.0
66-67	0.0	7.576597114722277E-4	0.0	0.41605559433109873	0.0
68-69	0.0	7.850450022483324E-4	0.0	0.4239151727838408	0.0
70-71	0.0	8.033018627657355E-4	0.0	0.43199383356279164	0.0
72-73	0.0	8.033018627657355E-4	0.0	0.4376625887534453	0.0
74-75	0.0	8.12430293024437E-4	0.0	0.44233634504590047	0.0
76-77	0.0	8.215587232831386E-4	0.0	0.4462980837781769	0.0
78-79	0.0	8.215587232831386E-4	0.0	0.4503876205340752	0.0
80-81	0.0	8.215587232831386E-4	0.0	0.4520489948411589	0.0
82-83	0.0	8.306871535418401E-4	0.0	0.45273362711056153	0.0
84-85	0.0	8.945861653527509E-4	0.0	0.4533726172286706	0.0
86-87	0.0	9.128430258701539E-4	0.0	0.4535643142641034	0.0
88-89	0.0	9.493567469049601E-4	0.0	0.45357344269436206	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6050	0.0	40.000492	1
CTTATAC	8535	0.0	34.11138	1
GTATCAA	10545	0.0	33.705936	1
TATACAC	9420	0.0	32.210728	3
TTATACA	10175	0.0	29.01577	2
ATCAACG	12330	0.0	27.896484	3
CAACGCA	12460	0.0	27.605932	5
TCAACGC	12650	0.0	27.22367	4
TATCAAC	13290	0.0	26.405176	2
AACGCAG	13070	0.0	26.317757	6
TACACAT	12550	0.0	24.14511	5
ACGCAGA	14630	0.0	23.48307	7
ATACACA	13915	0.0	22.747698	4
GTACATG	16960	0.0	22.502113	1
TACATGG	16720	0.0	22.433264	2
ACACATC	13060	0.0	22.230448	6
ACATGGG	16570	0.0	22.191523	3
CGCAGAG	15570	0.0	22.012737	8
CACATCT	14860	0.0	19.509748	7
CATGGGG	12550	0.0	19.120316	4
>>END_MODULE