FastQCFastQC Report
Fri 27 May 2016
SRR2140100_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140100_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6549236
Sequences flagged as poor quality0
Sequence length19-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76600.038.0589751
GTATCAA123200.032.184791
ATCAACG137800.027.924693
CAACGCA138050.027.8741235
TCAACGC140700.027.4085834
AACGCAG146050.026.5191276
TATCAAC148650.026.3087232
ACGCAGA163100.023.5162727
CGCAGAG171900.022.3124128
TACATGG177700.021.9842932
GTACATG184450.021.769361
ACATGGG177200.021.408853
CATGGGG133150.018.9734154
GCAGAGT212900.017.9172869
GTACTAG18400.017.0489081
GAGTACT118250.016.25314512-13
AGAGTAC184050.016.2375110-11
CAGAGTA204900.016.19787410-11
GTACTTT121300.015.86213814-15
GAGTACA132950.015.6358371
Produced by FastQC (version 0.11.5)