##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140100_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6549236
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73585926663812	34.0	31.0	34.0	31.0	34.0
2	32.94699305384628	34.0	31.0	34.0	31.0	34.0
3	33.05666325049212	34.0	33.0	34.0	31.0	34.0
4	36.44263056026688	37.0	37.0	37.0	35.0	37.0
5	36.29452519347295	37.0	37.0	37.0	35.0	37.0
6	36.318350720603135	37.0	37.0	37.0	35.0	37.0
7	36.32751621715877	37.0	37.0	37.0	35.0	37.0
8	36.33706053652671	37.0	37.0	37.0	35.0	37.0
9	38.2451146973479	39.0	39.0	39.0	37.0	39.0
10-11	38.149203968218586	39.0	38.5	39.0	37.0	39.0
12-13	38.13778798015524	39.0	38.5	39.0	37.0	39.0
14-15	39.645541479952776	41.0	40.0	41.0	37.0	41.0
16-17	39.62251658055993	41.0	39.5	41.0	37.0	41.0
18-19	39.65343461435807	41.0	40.0	41.0	37.5	41.0
20-21	39.66759313031805	41.0	40.0	41.0	37.5	41.0
22-23	39.62039819005004	41.0	40.0	41.0	37.0	41.0
24-25	39.60215081930994	41.0	39.5	41.0	37.0	41.0
26-27	39.49172737709442	41.0	39.0	41.0	37.0	41.0
28-29	39.42323838009279	40.5	39.0	41.0	37.0	41.0
30-31	39.3652550376992	40.0	39.0	41.0	36.5	41.0
32-33	39.28380546214025	40.0	39.0	41.0	36.0	41.0
34-35	39.24597407449406	40.0	39.0	41.0	36.0	41.0
36-37	39.20893712665277	40.0	39.0	41.0	36.0	41.0
38-39	39.14490166214759	40.0	39.0	41.0	35.0	41.0
40-41	39.075368212502106	40.0	39.0	41.0	35.0	41.0
42-43	39.064673021663566	40.0	39.0	41.0	35.0	41.0
44-45	39.003160696650546	40.0	39.0	41.0	35.0	41.0
46-47	38.88873405647462	40.0	38.0	41.0	35.0	41.0
48-49	38.87602758201217	40.0	38.0	41.0	35.0	41.0
50-51	38.76175687042319	40.0	38.0	41.0	35.0	41.0
52-53	38.588648452382586	40.0	38.0	41.0	35.0	41.0
54-55	38.387977780813834	40.0	37.5	41.0	34.5	41.0
56-57	38.23188645920406	40.0	37.0	41.0	34.0	41.0
58-59	38.054952322444535	39.5	37.0	41.0	34.0	41.0
60-61	37.81248450979515	39.0	36.0	41.0	34.0	41.0
62-63	37.632541593851265	39.0	36.0	41.0	34.0	41.0
64-65	37.33594587328341	39.0	35.0	40.0	33.0	41.0
66-67	37.07639539603424	38.0	35.0	40.0	33.0	41.0
68-69	36.79225439463431	37.0	35.0	40.0	33.0	41.0
70-71	36.33082616354503	37.0	35.0	39.0	32.5	41.0
72-73	36.05897541507022	36.0	35.0	39.0	32.0	41.0
74-75	35.70303848127776	36.0	35.0	39.0	32.0	40.0
76-77	33.92884841108558	34.5	32.0	36.5	29.0	39.0
78-79	34.9280005902174	35.0	34.0	37.0	31.0	39.0
80-81	34.870647168199255	35.0	34.0	36.5	32.0	39.0
82-83	34.62323263323664	35.0	34.0	36.0	31.5	37.5
84-85	34.40263036725073	35.0	34.0	36.0	31.0	37.0
86-87	34.23159776496732	35.0	34.0	35.5	31.0	37.0
88-89	34.084523886186375	35.0	34.0	35.0	31.0	36.0
90-91	34.03284728542445	35.0	34.0	35.0	31.0	36.0
92-93	33.92650246378966	35.0	34.0	35.0	31.0	36.0
94-95	33.78746506862025	35.0	34.0	35.0	31.0	36.0
96-97	33.8199263400975	35.0	34.0	35.0	31.0	35.0
98-99	33.85775108427781	35.0	34.0	35.0	31.0	35.0
100-101	32.63674973523466	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	8.0
20	35.0
21	74.0
22	260.0
23	887.0
24	2467.0
25	5623.0
26	10460.0
27	17525.0
28	26691.0
29	38370.0
30	53039.0
31	71061.0
32	96355.0
33	135804.0
34	217047.0
35	368191.0
36	765269.0
37	1684673.0
38	2092143.0
39	944254.0
40	18998.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.5161597474881	19.431289390090694	12.637122864407392	24.415427998013815
2	15.846708258940534	20.612774240498705	39.63127638421757	23.909241116343185
3	18.877674808568425	25.413336301631634	29.49315848468372	26.215830405116225
4	12.756098573940534	16.891435886567532	38.227497069887235	32.124968469604696
5	13.501193116265775	37.62962580673532	35.41405440268147	13.455126674317434
6	31.421071701833096	37.1105988510642	18.111702651518332	13.35662679558437
7	27.748595408685837	31.678046111027303	22.77743541384064	17.795923066446225
8	25.667833622120202	35.514737902253025	20.659905979873074	18.157522495753703
9	26.136216846122768	15.954232204865484	20.58602578561096	37.32352516340079
10-11	25.06275183377051	26.08087447480315	28.446701442356947	20.40967224906939
12-13	25.640183887771535	23.408083293074036	28.577896088872983	22.373836730281443
14-15	22.701296761942917	24.744359494756335	25.901540271262174	26.65280347203857
16-17	22.54755363831751	28.01741302344273	26.528697393100508	22.90633594513925
18-19	22.670560352383088	27.301161540063607	28.031315408392675	21.996962699160637
20-21	23.40469342300381	26.588546018863447	27.690413925574003	22.31634663255874
22-23	23.13851371239307	26.53684376011919	27.652433328159127	22.67220919932861
24-25	22.874092458071228	26.6523839978618	27.681698341940226	22.791825202126745
26-27	22.83644028186763	26.891153822386542	27.672642702688126	22.599763193057694
28-29	22.729489009529598	26.982964436469675	27.602114970467227	22.6854315835335
30-31	22.97146933793916	26.804044148975958	27.471949043349277	22.752537469735607
32-33	22.854878775388404	26.89564286327904	27.44718761158162	22.802290749750938
34-35	22.829234570351122	27.1504244640689	27.444133986760693	22.57620697881928
36-37	22.913935053199495	26.8461113488746	27.426563081613025	22.813390516312882
38-39	22.85947844867906	26.952482247537198	27.47403824946857	22.714001054315176
40-41	22.935772235647864	26.819638707021028	27.62522440337741	22.619364653953703
42-43	22.978968889236064	26.824926715875748	27.284509427824165	22.91159496706402
44-45	22.975930346537137	27.206571975731354	27.0201490966123	22.79734858111921
46-47	22.97245952282918	27.12099596536028	26.94773410484224	22.9588104069683
48-49	23.00958359801791	26.978847515847242	26.923976301212623	23.087592584922227
50-51	22.885916614149725	27.178214866164712	26.86244983249859	23.073418687186972
52-53	22.94385695565922	27.250900078606378	26.675611963413065	23.12963100232134
54-55	23.117054262319918	26.989742610611472	26.719030366112488	23.17417276095612
56-57	23.171749884231	27.001264372780025	26.90617772454803	22.920808018440944
58-59	23.050712333404224	26.999886616147574	26.970628220056003	22.9787728303922
60-61	23.144025925450336	27.191885980641985	26.55304804934569	23.111040044561992
62-63	23.14167233085736	27.267979980689578	26.577125583730005	23.01322210472306
64-65	22.956652961187093	27.104593130659786	26.726802389138314	23.2119515190148
66-67	23.0872528229535	27.092680326528708	26.548917712296216	23.271149138221574
68-69	23.041608996286662	27.09727542203013	26.705814856865285	23.155300724817916
70-71	23.11812212697908	26.85762847967225	26.643743277040805	23.380506116307863
72-73	23.089948721368618	27.015984156712474	26.643158496940778	23.25090862497813
74-75	22.79585861734838	27.151363828273407	26.787135709123728	23.265641845254486
76-77	23.11668526218337	27.043985560181195	26.56787133608189	23.27145784155355
78-79	23.073836054307463	27.07664902628527	26.559567144386463	23.289947775020806
80-81	23.014956718703182	27.14197553408017	26.600198469966983	23.242869277249664
82-83	23.31868363257568	26.967173926680843	26.78421753309405	22.929924907649422
84-85	23.300405559282318	26.929440211071537	26.619880162449093	23.15027406719705
86-87	22.99504356057018	27.10826906338918	26.806845520536132	23.089841855504503
88-89	23.20481229313153	26.840746474791054	26.758891282979054	23.195549949098364
90-91	23.260318253058994	27.04072882903571	26.563930650731844	23.135022267173454
92-93	23.274847831872098	27.002250742855495	26.63153391330035	23.09136751197206
94-95	23.116024263167215	27.024192509507554	26.73152311734909	23.128260109976136
96-97	22.986700903303237	26.976966868799728	26.862003393345706	23.174328834551332
98-99	23.37129652732834	27.549362268676557	26.846428029270765	22.23291317472434
100-101	27.9297590281335	34.70684379885548	27.090494360993837	10.272902812017183
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	798.0
1	783.5
2	1325.5
3	2018.0
4	2965.5
5	5114.0
6	6791.5
7	7680.0
8	7994.5
9	7432.0
10	6418.0
11	5540.5
12	6031.5
13	8346.0
14	13517.5
15	20037.5
16	24918.5
17	27499.5
18	27936.0
19	26488.0
20	24137.0
21	22200.0
22	21881.5
23	24512.5
24	30104.5
25	37740.5
26	47723.5
27	59369.5
28	70781.0
29	82158.5
30	93159.0
31	103930.0
32	116476.0
33	128604.0
34	139603.5
35	153093.5
36	169562.0
37	183562.5
38	191387.5
39	197845.0
40	206623.0
41	215265.5
42	223899.5
43	235588.0
44	251586.0
45	268921.5
46	277661.0
47	283910.5
48	298252.0
49	308427.0
50	312836.5
51	312565.5
52	302881.5
53	293018.5
54	280275.5
55	263116.0
56	246035.0
57	228323.5
58	206617.5
59	175976.5
60	142351.0
61	112730.0
62	88308.0
63	67681.0
64	51650.5
65	39491.0
66	29630.0
67	22379.0
68	16943.5
69	12916.0
70	9775.0
71	7160.5
72	5200.5
73	3848.5
74	2797.0
75	1956.5
76	1272.5
77	783.0
78	516.5
79	371.5
80	255.0
81	164.0
82	114.5
83	82.5
84	58.0
85	39.0
86	32.0
87	24.5
88	12.5
89	3.5
90	2.0
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001572702525912946
3	4.4279974030558676E-4
4	0.0
5	0.0
6	6.871030453017726E-4
7	0.0
8	0.0
9	3.053791312452323E-5
10-11	3.8172391405654035E-4
12-13	3.6645495749427874E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.34690571510892E-5
28-29	7.643550074348812E-6
30-31	3.062518638297025E-5
32-33	2.3031199060265662E-4
34-35	5.39241364434721E-5
36-37	7.432204360845908E-4
38-39	1.3262789854371447E-4
40-41	8.656619374505738E-5
42-43	0.0
44-45	8.017498029098547E-6
46-47	0.0
48-49	8.201974608983164E-6
50-51	8.29214830573582E-6
52-53	0.0
54-55	6.006204155108953E-4
56-57	1.1117232240178737E-4
58-59	1.7297304777212877E-5
60-61	9.18452846062317E-4
62-63	8.843388280547297E-6
64-65	8.93777254620158E-6
66-67	3.6152012343020054E-5
68-69	0.0
70-71	0.0
72-73	9.366293444849672E-5
74-75	0.0
76-77	1.9165692490488787E-5
78-79	5.817531382915442E-5
80-81	0.0
82-83	0.0
84-85	3.0185265082973255E-5
86-87	5.907035482951074E-4
88-89	1.2377281829263238E-4
90-91	0.0
92-93	2.1221406542092768E-5
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	134.0
20-21	430.0
22-23	816.0
24-25	1464.0
26-27	3254.0
28-29	8430.0
30-31	17066.0
32-33	20807.0
34-35	27980.0
36-37	45745.0
38-39	56117.0
40-41	54549.0
42-43	59286.0
44-45	69831.0
46-47	70077.0
48-49	67675.0
50-51	61492.0
52-53	58212.0
54-55	62626.0
56-57	65788.0
58-59	64846.0
60-61	63511.0
62-63	59901.0
64-65	60921.0
66-67	64227.0
68-69	65475.0
70-71	65047.0
72-73	60546.0
74-75	60367.0
76-77	60008.0
78-79	63518.0
80-81	64399.0
82-83	60529.0
84-85	59775.0
86-87	60625.0
88-89	65348.0
90-91	69052.0
92-93	69410.0
94-95	74641.0
96-97	118082.0
98-99	532717.0
100-101	3934512.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.41053624395063
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.59791224565738	73.01401458259106
2	7.548011674063184	12.44071379270293
3	2.0564404176586377	5.0841707271894645
4	0.7702696041635203	2.539133245261252
5	0.37682508243698515	1.5527178556901422
6	0.20538062651835592	1.0155316539297754
7	0.12816589227735853	0.7393553937533069
8	0.0772873903060482	0.5095436224013554
9	0.060592396151219564	0.4494106673215105
>10	0.17439181439216223	2.2004028383667165
>50	0.0034869739547958687	0.1845318496304985
>100	0.0010669605914048366	0.1806280362414456
>500	1.689218289631755E-4	0.08984573492058773
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	6.107582624904646E-5	0.0	0.0	0.0
5	0.0	6.107582624904646E-5	0.0	0.0	0.0
6	0.0	6.107582624904646E-5	0.0	0.0	0.0
7	0.0	6.107582624904646E-5	0.0	0.0	0.0
8	0.0	6.107582624904646E-5	0.0	0.0	0.0
9	0.0	1.2215165249809291E-4	0.0	0.0	3.053791312452323E-5
10-11	0.0	1.2215165249809291E-4	0.0	0.0	3.053791312452323E-5
12-13	1.5268956562261614E-5	1.2215165249809291E-4	0.0	0.0	3.053791312452323E-5
14-15	2.290343484339242E-5	1.2978613077922372E-4	0.0	7.634478281130807E-6	9.161373937356969E-5
16-17	3.053791312452323E-5	1.3742060906035453E-4	0.0	1.5268956562261614E-5	1.068826959358313E-4
18-19	3.053791312452323E-5	1.3742060906035453E-4	0.0	5.344134796791565E-5	1.2215165249809291E-4
20-21	3.053791312452323E-5	1.3742060906035453E-4	0.0	7.634478281130807E-5	1.2215165249809291E-4
22-23	3.053791312452323E-5	1.5268956562261614E-4	0.0	9.161373937356969E-5	1.5268956562261614E-4
24-25	3.053791312452323E-5	2.137653918716626E-4	0.0	1.2215165249809291E-4	1.6795852218487776E-4
26-27	3.053791312452323E-5	2.4430330499618583E-4	0.0	1.6032404390374695E-4	1.6795852218487776E-4
28-29	3.053791312452323E-5	2.4430330499618583E-4	0.0	6.183927407715954E-4	1.6795852218487776E-4
30-31	3.053791312452323E-5	2.9011017468297067E-4	0.0	0.002015502266218533	1.6795852218487776E-4
32-33	3.053791312452323E-5	2.9011017468297067E-4	0.0	0.004344018141963429	1.8322747874713937E-4
34-35	3.053791312452323E-5	3.206480878074939E-4	0.0	0.00700845106207808	1.8322747874713937E-4
36-37	3.053791312452323E-5	3.511860009320171E-4	0.0	0.011398276073728293	1.9086195702827018E-4
38-39	3.053791312452323E-5	3.511860009320171E-4	0.0	0.018025003221749835	2.061309135905318E-4
40-41	3.053791312452323E-5	3.9699287061880197E-4	0.0	0.02611755019974849	2.137653918716626E-4
42-43	3.053791312452323E-5	3.9699287061880197E-4	0.0	0.03337793904510389	2.137653918716626E-4
44-45	3.053791312452323E-5	4.046273488999328E-4	0.0	0.04143231363169689	2.137653918716626E-4
46-47	3.053791312452323E-5	4.122618271810636E-4	0.0	0.049746260479848335	2.290343484339242E-4
48-49	3.053791312452323E-5	4.122618271810636E-4	0.0	0.05847246915518085	2.290343484339242E-4
50-51	3.053791312452323E-5	4.504342185867176E-4	0.0	0.0670383537866096	2.290343484339242E-4
52-53	3.053791312452323E-5	4.580686968678484E-4	0.0	0.07511563180804601	2.3666882671505502E-4
54-55	3.053791312452323E-5	4.580686968678484E-4	0.0	0.08311656504667109	2.4430330499618583E-4
56-57	3.053791312452323E-5	4.580686968678484E-4	0.0	0.09059835376217928	2.4430330499618583E-4
58-59	3.053791312452323E-5	4.7333765343011004E-4	0.0	0.09743121182379136	2.5193778327731663E-4
60-61	3.053791312452323E-5	4.886066099923717E-4	0.0	0.10421826301571664	2.7484121812070906E-4
62-63	3.053791312452323E-5	4.886066099923717E-4	0.0	0.1103563835537458	2.9011017468297067E-4
64-65	3.053791312452323E-5	4.886066099923717E-4	0.0	0.11687622800583153	2.9011017468297067E-4
66-67	3.053791312452323E-5	4.886066099923717E-4	0.0	0.12290746584792486	2.9011017468297067E-4
68-69	3.053791312452323E-5	4.886066099923717E-4	0.0	0.1291830069950144	2.9011017468297067E-4
70-71	3.053791312452323E-5	4.886066099923717E-4	0.0	0.1356570445774133	3.206480878074939E-4
72-73	3.053791312452323E-5	4.886066099923717E-4	0.0	0.1408103174171766	3.9699287061880197E-4
74-75	3.053791312452323E-5	5.038755665546333E-4	0.0	0.1447039013405533	3.9699287061880197E-4
76-77	3.053791312452323E-5	5.420479579602873E-4	0.0	0.1481241476104999	3.9699287061880197E-4
78-79	3.053791312452323E-5	5.573169145225489E-4	0.0	0.15142987670622954	3.9699287061880197E-4
80-81	3.053791312452323E-5	5.649513928036797E-4	0.0	0.15281935175339537	4.198963054621944E-4
82-83	3.053791312452323E-5	5.649513928036797E-4	0.0	0.15355989614666504	4.427997403055868E-4
84-85	3.053791312452323E-5	5.802203493659413E-4	0.0	0.15395688901728383	4.427997403055868E-4
86-87	3.053791312452323E-5	5.95489305928203E-4	0.0	0.15408667514806307	4.427997403055868E-4
88-89	3.053791312452323E-5	6.565651321772494E-4	0.0	0.15415538545259325	4.427997403055868E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7660	0.0	38.058975	1
GTATCAA	12320	0.0	32.18479	1
ATCAACG	13780	0.0	27.92469	3
CAACGCA	13805	0.0	27.874123	5
TCAACGC	14070	0.0	27.408583	4
AACGCAG	14605	0.0	26.519127	6
TATCAAC	14865	0.0	26.308723	2
ACGCAGA	16310	0.0	23.516272	7
CGCAGAG	17190	0.0	22.312412	8
TACATGG	17770	0.0	21.984293	2
GTACATG	18445	0.0	21.76936	1
ACATGGG	17720	0.0	21.40885	3
CATGGGG	13315	0.0	18.973415	4
GCAGAGT	21290	0.0	17.917286	9
GTACTAG	1840	0.0	17.048908	1
GAGTACT	11825	0.0	16.253145	12-13
AGAGTAC	18405	0.0	16.23751	10-11
CAGAGTA	20490	0.0	16.197874	10-11
GTACTTT	12130	0.0	15.862138	14-15
GAGTACA	13295	0.0	15.635837	1
>>END_MODULE