FastQCFastQC Report
Fri 27 May 2016
SRR2140099_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140099_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4847973
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80450.042.756721
CTTATAC55800.038.1156771
TATACAC63050.036.132673
GTATCAA135650.033.1369361
TTATACA71250.030.7116682
CAACGCA160000.027.4806775
ATCAACG161950.027.2755033
TCAACGC165300.026.8222584
TACACAT85300.026.756185
TATCAAC167350.026.7133832
AACGCAG167800.026.3500636
TACATGG197150.024.8538952
ACACATC87800.024.7836346
ATACACA98700.024.3668354
GTACATG204700.024.3168411
GTACTAG14300.024.025791
ACATGGG200700.023.9860693
ACGCAGA193050.022.7343377
ACATCTC97750.022.5958868
CATGGGG134150.022.3182094
Produced by FastQC (version 0.11.5)