##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2140099_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4847973 Sequences flagged as poor quality 0 Sequence length 19-101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.72587883637141 34.0 31.0 34.0 31.0 34.0 2 32.927665232459006 34.0 31.0 34.0 31.0 34.0 3 33.03404701305061 34.0 33.0 34.0 31.0 34.0 4 36.423515560008276 37.0 37.0 37.0 35.0 37.0 5 36.28142256567848 37.0 37.0 37.0 35.0 37.0 6 36.31139096690514 37.0 37.0 37.0 35.0 37.0 7 36.315734637961064 37.0 37.0 37.0 35.0 37.0 8 36.32809671175974 37.0 37.0 37.0 35.0 37.0 9 38.226583151350056 39.0 39.0 39.0 37.0 39.0 10-11 38.13291379716843 39.0 38.5 39.0 37.0 39.0 12-13 38.117318310147354 39.0 38.5 39.0 37.0 39.0 14-15 39.589906647582396 41.0 39.0 41.0 37.0 41.0 16-17 39.56497395921966 41.0 39.0 41.0 37.0 41.0 18-19 39.615969808412714 41.0 39.5 41.0 37.0 41.0 20-21 39.638892948744356 41.0 40.0 41.0 37.0 41.0 22-23 39.58093324909537 41.0 39.0 41.0 37.0 41.0 24-25 39.553505531631004 41.0 39.0 41.0 37.0 41.0 26-27 39.4327045933924 41.0 39.0 41.0 37.0 41.0 28-29 39.36565016985449 40.0 39.0 41.0 36.0 41.0 30-31 39.2891142677255 40.0 39.0 41.0 36.0 41.0 32-33 39.18471092915571 40.0 39.0 41.0 35.5 41.0 34-35 39.12909472795978 40.0 39.0 41.0 35.0 41.0 36-37 39.0961053282092 40.0 39.0 41.0 35.0 41.0 38-39 39.021831183247635 40.0 39.0 41.0 35.0 41.0 40-41 38.966614348332094 40.0 38.0 41.0 35.0 41.0 42-43 38.958235133495904 40.0 38.0 41.0 35.0 41.0 44-45 38.89381809722438 40.0 38.0 41.0 35.0 41.0 46-47 38.780914575966115 40.0 38.0 41.0 35.0 41.0 48-49 38.772496439030114 40.0 38.0 41.0 35.0 41.0 50-51 38.64164455263767 40.0 38.0 41.0 35.0 41.0 52-53 38.46558026820438 40.0 38.0 41.0 34.5 41.0 54-55 38.245294322050384 40.0 37.0 41.0 34.0 41.0 56-57 38.07299008923591 40.0 37.0 41.0 34.0 41.0 58-59 37.88960265902706 39.0 36.0 41.0 34.0 41.0 60-61 37.62431364265454 39.0 36.0 41.0 33.5 41.0 62-63 37.44124003826555 39.0 35.0 41.0 33.0 41.0 64-65 37.1615243974705 38.5 35.0 40.0 33.0 41.0 66-67 36.917799088982285 37.5 35.0 40.0 33.0 41.0 68-69 36.648685516898844 37.0 35.0 40.0 33.0 41.0 70-71 36.20308310120533 36.5 35.0 39.0 32.0 41.0 72-73 35.94285721393098 36.0 35.0 39.0 32.0 41.0 74-75 35.59639579660055 35.5 35.0 38.5 32.0 40.0 76-77 33.84209285707671 34.5 32.0 36.5 29.0 39.0 78-79 34.86014983857229 35.0 34.0 37.0 31.0 39.0 80-81 34.81937715937427 35.0 34.0 36.5 32.0 39.0 82-83 34.58571115056144 35.0 34.0 36.0 31.5 37.5 84-85 34.372264993992715 35.0 34.0 36.0 31.0 37.0 86-87 34.20985970614956 35.0 34.0 35.0 31.0 37.0 88-89 34.06668619261124 35.0 34.0 35.0 31.0 36.0 90-91 34.01938326884671 35.0 34.0 35.0 31.0 36.0 92-93 33.91212127934945 35.0 34.0 35.0 31.0 36.0 94-95 33.77399731753397 35.0 34.0 35.0 31.0 36.0 96-97 33.80775171576582 35.0 34.0 35.0 31.0 35.0 98-99 33.84668913134966 35.0 34.0 35.0 31.0 35.0 100-101 32.60684561198928 34.0 31.5 35.0 28.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 3.0 18 5.0 19 3.0 20 18.0 21 73.0 22 238.0 23 763.0 24 1956.0 25 4484.0 26 8303.0 27 13631.0 28 20694.0 29 29264.0 30 40000.0 31 53652.0 32 72523.0 33 103870.0 34 166552.0 35 282835.0 36 591773.0 37 1263162.0 38 1481605.0 39 697267.0 40 15299.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.405852301570164 19.660423026283354 12.796214005317275 24.137510666829208 2 15.678037685938412 20.529448881981903 40.17666173189307 23.615851700186617 3 18.588914927423858 26.24506930052602 29.984180919618897 25.18183485243123 4 12.48395979103019 17.29832241227416 37.778263204023624 32.43945459267203 5 13.099206616868534 38.11539379447864 35.19483297452358 13.590566614129246 6 30.62064145849629 37.18654805163087 18.230192873196767 13.962617616676074 7 27.232433018913266 32.22507633602745 22.420958202531242 18.121532442528043 8 25.914397625564334 35.24006837496826 20.664162114764252 18.181371884703154 9 26.27837501503206 15.57715588645229 20.45043173731856 37.69403736119708 10-11 25.072355250822255 26.058172982216217 28.149033974118986 20.72043779284255 12-13 25.801310099740064 23.52540036755731 28.352583560293454 22.320705972409172 14-15 22.963597363269145 24.743547457875696 25.786292539170496 26.506562639684667 16-17 22.458066495007294 28.203209877612768 26.3163903759365 23.02233325144344 18-19 22.44983625115074 27.027079152462274 28.37016625298862 22.152918343398365 20-21 23.34172480670526 26.12645422294503 27.97710621237194 22.554714757977777 22-23 23.15025088162373 26.344272468047198 27.94274799239 22.56272865793907 24-25 22.872517126785464 26.590394474200156 28.114689728798215 22.422398670216168 26-27 22.697975153319376 26.708277662063633 28.24088832409518 22.35285886052181 28-29 22.302498672512122 26.583191323202527 28.40323116901252 22.711078835272826 30-31 22.703957748467257 26.596459277174873 27.825221419377595 22.874361554980275 32-33 22.778992140276692 26.65639333299412 28.196105755801337 22.36850877092785 34-35 22.676412325097967 27.067286603539266 28.06300450981032 22.19329656155245 36-37 22.58223463750432 26.81421945556373 27.80717282504794 22.796373081884017 38-39 22.588990694808647 26.628419069938047 28.09435235017304 22.688237885080262 40-41 22.546919774067362 26.52350656247013 28.34676780355127 22.582805859911243 42-43 22.863574858220733 26.495957391767217 27.97191840136137 22.668549348650675 44-45 22.65174587621305 27.05521014055457 27.609292482079965 22.68375150115241 46-47 23.058247067785054 26.8013516954724 27.112124038198548 23.028277198544004 48-49 23.00634406307365 26.691437961754723 27.058766087551017 23.243451887620612 50-51 22.654221967943826 26.961376340066458 27.14044506190533 23.24395663008439 52-53 22.76265708444174 27.122364978455906 27.171558887772633 22.94341904932972 54-55 22.845727369680656 27.058596626001158 26.836133882505436 23.259542121812753 56-57 23.20841870804059 27.1565869584066 26.65675363251513 22.97824070103768 58-59 22.745447495029484 27.43682275882436 27.146591510325813 22.671138235820347 60-61 23.125236173144202 27.832884338655074 26.258967595253008 22.782911892947716 62-63 22.81398242001793 27.964014522626023 26.295997258803204 22.926005798552836 64-65 22.718756124681516 27.685635346961956 26.504946742769377 23.090661785587148 66-67 22.884529567442787 27.458483331141853 26.436932891517763 23.220054209897594 68-69 23.028998798353882 27.362580090409566 26.559575304960877 23.048845806275676 70-71 23.040660750930346 26.954266526094717 26.63111576895159 23.37395695402335 72-73 23.035383102912398 27.102023917409472 26.67021493437251 23.192378045305617 74-75 22.7837329117171 27.20409940267603 26.80995497889212 23.20221270671475 76-77 23.050011529784914 27.101860755005124 26.550497738639358 23.297629976570608 78-79 23.11565578141125 27.099256940435364 26.54743911058815 23.237648167565236 80-81 22.936357414864204 27.118488208924585 26.695613006930714 23.249541369280497 82-83 23.31722805021355 26.926265060514247 26.83533525863319 22.921171630639005 84-85 23.336986505296764 26.84064041967747 26.638334107602702 23.184038967423064 86-87 22.99792950287967 27.06527499272398 26.82214380209928 23.114651702297063 88-89 23.262017231933743 26.706040041581687 26.757987602321627 23.273955124162942 90-91 23.279783576066958 26.74464634981193 26.710567503576705 23.265002570544414 92-93 23.360821738291364 26.74957684230921 26.75104139889563 23.138560020503792 94-95 23.114975912559814 26.78493252817441 26.822133327789928 23.27795823147585 96-97 23.058133947028946 26.730908186725987 26.900619161495403 23.310338704749665 98-99 23.450130787290387 27.3349460125764 26.843374973869636 22.37154822626357 100-101 27.96336936848381 34.347830732377574 27.2881964403625 10.400603458776116 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 513.0 1 621.0 2 1402.5 3 2187.0 4 3281.5 5 6047.5 6 8175.5 7 9211.5 8 9527.5 9 8686.0 10 7231.0 11 5940.5 12 6496.0 13 9456.5 14 16471.0 15 25585.5 16 32301.0 17 35700.0 18 35688.0 19 32623.0 20 28101.5 21 23591.0 22 20757.0 23 21224.5 24 24611.5 25 29969.5 26 37222.5 27 45768.0 28 53853.5 29 62002.0 30 70143.5 31 77408.0 32 85671.0 33 94240.5 34 102573.5 35 112544.5 36 123625.0 37 134129.0 38 140525.5 39 144518.0 40 151067.0 41 157836.5 42 163769.5 43 172380.0 44 184194.5 45 197969.5 46 207346.5 47 213909.0 48 230288.5 49 240384.0 50 237887.5 51 236020.0 52 229683.5 53 221146.5 54 210491.5 55 196617.0 56 183722.0 57 170320.5 58 153873.0 59 132438.5 60 108820.5 61 86714.5 62 67519.5 63 51775.5 64 39419.5 65 29482.5 66 21837.0 67 16484.0 68 12377.0 69 9182.5 70 6818.0 71 4995.5 72 3527.5 73 2512.0 74 1856.0 75 1362.5 76 900.5 77 559.5 78 387.0 79 271.0 80 190.0 81 120.0 82 75.0 83 53.0 84 32.0 85 22.5 86 25.0 87 22.0 88 14.5 89 8.0 90 3.5 91 2.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0013201393654626377 3 6.188153275606114E-4 4 0.0 5 0.0 6 4.33170729292428E-4 7 0.0 8 0.0 9 4.125435517070743E-5 10-11 3.7128919653636684E-4 12-13 4.4348431808510487E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.0959528672135494E-5 28-29 1.0328634424388302E-5 30-31 2.0700166574240422E-5 32-33 1.6614528200357918E-4 34-35 4.1706318079699315E-5 36-37 6.083143567222511E-4 38-39 1.7991034326787667E-4 40-41 6.415863436208138E-5 42-43 0.0 44-45 1.093835319154894E-5 46-47 0.0 48-49 0.0 50-51 3.419080841659175E-5 52-53 0.0 54-55 6.063524750258574E-4 56-57 9.449975961623648E-5 58-59 0.0 60-61 7.652224611011916E-4 62-63 2.464218014296161E-5 64-65 1.2499348471460924E-5 66-67 3.807586412856341E-5 68-69 0.0 70-71 0.0 72-73 6.62649157355452E-5 74-75 1.3422707086196062E-5 76-77 2.7192881556237734E-5 78-79 6.889106737753063E-5 80-81 0.0 82-83 0.0 84-85 1.436936451485433E-5 86-87 4.078632539388665E-4 88-89 1.034222267071353E-4 90-91 3.001218494708852E-5 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 118.0 20-21 415.0 22-23 693.0 24-25 1245.0 26-27 2979.0 28-29 8001.0 30-31 15218.0 32-33 18282.0 34-35 24839.0 36-37 39392.0 38-39 49097.0 40-41 48019.0 42-43 53352.0 44-45 63710.0 46-47 63123.0 48-49 58965.0 50-51 51703.0 52-53 47916.0 54-55 53573.0 56-57 57618.0 58-59 57971.0 60-61 59592.0 62-63 57621.0 64-65 58710.0 66-67 62073.0 68-69 56027.0 70-71 52788.0 72-73 48330.0 74-75 47424.0 76-77 47702.0 78-79 50848.0 80-81 51558.0 82-83 47906.0 84-85 46607.0 86-87 47898.0 88-89 51253.0 90-91 54439.0 92-93 54187.0 94-95 55338.0 96-97 83656.0 98-99 367383.0 100-101 2730404.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.8553972232017 #Duplication Level Percentage of deduplicated Percentage of total 1 88.66786519116167 73.46611191347 2 7.527377778366789 12.47367751751364 3 2.032570171921005 5.05228226935639 4 0.8015892478425584 2.6566398215937066 5 0.37753745094279356 1.5640507732250095 6 0.1921032917553371 0.9550076727763842 7 0.11395472763512161 0.6609234956568834 8 0.07759339725436369 0.5143225401126394 9 0.04903183090865397 0.3656296643865646 >10 0.15755599458977368 1.9221429389054412 >50 0.001645434999929829 0.0854415164257347 >100 9.589088206160471E-4 0.16684476342429994 >500 2.1657380140181865E-4 0.11692511315330684 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.0627177585353714E-5 0.0 4 0.0 0.0 0.0 4.125435517070743E-5 0.0 5 0.0 0.0 0.0 4.125435517070743E-5 0.0 6 2.0627177585353714E-5 0.0 0.0 4.125435517070743E-5 2.0627177585353714E-5 7 2.0627177585353714E-5 0.0 0.0 4.125435517070743E-5 2.0627177585353714E-5 8 2.0627177585353714E-5 0.0 0.0 4.125435517070743E-5 2.0627177585353714E-5 9 2.0627177585353714E-5 0.0 0.0 1.0313588792676856E-4 2.0627177585353714E-5 10-11 6.188153275606114E-5 0.0 0.0 1.44390243097476E-4 2.0627177585353714E-5 12-13 6.188153275606114E-5 0.0 0.0 1.5470383189015286E-4 2.0627177585353714E-5 14-15 7.2195121548738E-5 0.0 0.0 1.9595818706086027E-4 4.125435517070743E-5 16-17 1.237630655121223E-4 0.0 0.0 2.2689895343889086E-4 6.188153275606114E-5 18-19 1.650174206828297E-4 0.0 0.0 2.372125422315677E-4 8.250871034141486E-5 20-21 1.650174206828297E-4 0.0 0.0 3.300348413656594E-4 8.250871034141486E-5 22-23 1.8564459826818342E-4 0.0 0.0 3.5066201895101313E-4 8.250871034141486E-5 24-25 2.1658536464621398E-4 0.0 0.0 4.5379790687778167E-4 1.2376306551212227E-4 26-27 2.372125422315677E-4 0.0 0.0 5.363066172191966E-4 1.44390243097476E-4 28-29 2.4752613102424454E-4 0.0 0.0 0.0012582578327065765 1.44390243097476E-4 30-31 3.300348413656594E-4 0.0 0.0 0.0031043902265957338 1.44390243097476E-4 32-33 3.300348413656594E-4 0.0 0.0 0.005754982546313686 1.650174206828297E-4 34-35 3.816027853290437E-4 0.0 0.0 0.00899344942721422 1.650174206828297E-4 36-37 4.4348431808510487E-4 0.0 0.0 0.013789268215808958 1.650174206828297E-4 38-39 4.537979068777817E-4 0.0 0.0 0.021710104408584785 1.650174206828297E-4 40-41 4.537979068777817E-4 0.0 0.0 0.029857839554799502 1.8564459826818342E-4 42-43 4.537979068777817E-4 0.0 0.0 0.037809616513953356 1.8564459826818342E-4 44-45 4.847386732558123E-4 0.0 0.0 0.045596376052424385 1.8564459826818342E-4 46-47 5.156794396338428E-4 0.0 0.0 0.05468264777877269 1.9595818706086027E-4 48-49 5.156794396338428E-4 0.0 0.0 0.06331512159824322 2.2689895343889086E-4 50-51 5.156794396338428E-4 6.188153275606114E-5 0.0 0.07253546997889634 2.2689895343889086E-4 52-53 5.878745611825808E-4 6.188153275606114E-5 0.0 0.08102355355526938 2.681533086095983E-4 54-55 5.981881499752577E-4 6.188153275606114E-5 0.0 0.08937756047733764 3.1972125257298257E-4 56-57 5.981881499752577E-4 6.188153275606114E-5 0.0 0.09808222941835691 3.300348413656594E-4 58-59 5.981881499752577E-4 6.188153275606114E-5 0.0 0.10736445933176608 3.300348413656594E-4 60-61 6.600696827313189E-4 6.188153275606114E-5 0.0 0.11588348367451717 3.9191637412172055E-4 62-63 6.600696827313189E-4 6.188153275606114E-5 0.0 0.12476348362501194 4.1254355170707426E-4 64-65 6.600696827313189E-4 6.188153275606114E-5 0.0 0.13247804804193425 4.1254355170707426E-4 66-67 6.600696827313189E-4 6.188153275606114E-5 0.0 0.1394500340657838 4.1254355170707426E-4 68-69 6.600696827313189E-4 6.188153275606114E-5 0.0 0.14689644517409647 4.1254355170707426E-4 70-71 6.600696827313189E-4 6.188153275606114E-5 0.0 0.15387874478673869 4.1254355170707426E-4 72-73 6.600696827313189E-4 6.188153275606114E-5 0.0 0.15987093987528395 4.537979068777817E-4 74-75 6.600696827313189E-4 7.2195121548738E-5 0.0 0.16463581789750067 4.537979068777817E-4 76-77 6.806968603166726E-4 9.282229913409171E-5 0.0 0.16864780393785195 4.537979068777817E-4 78-79 7.013240379020263E-4 1.0313588792676856E-4 0.0 0.1721544241273621 4.537979068777817E-4 80-81 7.013240379020263E-4 1.0313588792676856E-4 0.0 0.1735364450255808 4.537979068777817E-4 82-83 7.013240379020263E-4 1.0313588792676856E-4 0.0 0.17405212446521465 4.7442508446313543E-4 84-85 7.013240379020263E-4 1.0313588792676856E-4 0.0 0.17452654954967778 4.7442508446313543E-4 86-87 7.013240379020263E-4 1.0313588792676856E-4 0.0 0.1746503126151899 4.7442508446313543E-4 88-89 7.013240379020263E-4 1.2376306551212227E-4 0.0 0.17467093979277526 4.7442508446313543E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8045 0.0 42.75672 1 CTTATAC 5580 0.0 38.115677 1 TATACAC 6305 0.0 36.13267 3 GTATCAA 13565 0.0 33.136936 1 TTATACA 7125 0.0 30.711668 2 CAACGCA 16000 0.0 27.480677 5 ATCAACG 16195 0.0 27.275503 3 TCAACGC 16530 0.0 26.822258 4 TACACAT 8530 0.0 26.75618 5 TATCAAC 16735 0.0 26.713383 2 AACGCAG 16780 0.0 26.350063 6 TACATGG 19715 0.0 24.853895 2 ACACATC 8780 0.0 24.783634 6 ATACACA 9870 0.0 24.366835 4 GTACATG 20470 0.0 24.316841 1 GTACTAG 1430 0.0 24.02579 1 ACATGGG 20070 0.0 23.986069 3 ACGCAGA 19305 0.0 22.734337 7 ACATCTC 9775 0.0 22.595886 8 CATGGGG 13415 0.0 22.318209 4 >>END_MODULE