FastQCFastQC Report
Fri 27 May 2016
SRR2140098_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140098_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6721723
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108600.038.7914161
GTATCAA185350.033.5650941
ATCAACG219050.027.507593
CAACGCA220100.027.3219365
AACGCAG231200.026.459646
TATCAAC234250.026.387872
TCAACGC231600.026.154934
ACGCAGA260550.023.0807637
TACATGG264750.023.076422
CGCAGAG263200.022.8483758
GTACATG273900.022.6262571
ACATGGG266600.022.1970163
GCAGAGT304450.019.7001789
CATGGGG187600.019.6673414
GTACTAG27450.018.7655091
CGACGAG11150.018.51700424-25
ACGGTAC7350.018.4713293
GGTTCAC25250.017.7120846
GAGTACT164550.017.67833712-13
GGCGTCG16650.017.2676988
Produced by FastQC (version 0.11.5)