##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140098_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6721723
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67051245640441	34.0	31.0	34.0	31.0	34.0
2	32.896131542463145	34.0	31.0	34.0	31.0	34.0
3	33.00803796883626	34.0	33.0	34.0	31.0	34.0
4	36.41711135671613	37.0	37.0	37.0	35.0	37.0
5	36.25098044058049	37.0	37.0	37.0	35.0	37.0
6	36.27148143415014	37.0	37.0	37.0	35.0	37.0
7	36.28780269582665	37.0	37.0	37.0	35.0	37.0
8	36.30702886744961	37.0	37.0	37.0	35.0	37.0
9	38.244668963597576	39.0	39.0	39.0	37.0	39.0
10-11	38.14443023016569	39.0	38.5	39.0	37.0	39.0
12-13	38.1322045701675	39.0	39.0	39.0	37.0	39.0
14-15	39.60685005912919	41.0	39.0	41.0	37.0	41.0
16-17	39.58856025456568	41.0	39.0	41.0	37.0	41.0
18-19	39.64158534947066	41.0	39.5	41.0	37.0	41.0
20-21	39.66926553040621	41.0	40.0	41.0	37.0	41.0
22-23	39.62108478830362	41.0	40.0	41.0	37.0	41.0
24-25	39.594647059642355	41.0	39.5	41.0	37.0	41.0
26-27	39.47836470136761	41.0	39.0	41.0	37.0	41.0
28-29	39.414235532372246	40.0	39.0	41.0	36.5	41.0
30-31	39.334861822759244	40.0	39.0	41.0	36.0	41.0
32-33	39.240150579115294	40.0	39.0	41.0	35.5	41.0
34-35	39.204768644562634	40.0	39.0	41.0	35.0	41.0
36-37	39.16600308585927	40.0	39.0	41.0	35.0	41.0
38-39	39.10171223557019	40.0	39.0	41.0	35.0	41.0
40-41	39.03708325583557	40.0	39.0	41.0	35.0	41.0
42-43	39.0255650584146	40.0	39.0	41.0	35.0	41.0
44-45	38.96020960182058	40.0	38.0	41.0	35.0	41.0
46-47	38.8457880287591	40.0	38.0	41.0	35.0	41.0
48-49	38.83178603021048	40.0	38.0	41.0	35.0	41.0
50-51	38.72022165148684	40.0	38.0	41.0	35.0	41.0
52-53	38.5503747845725	40.0	38.0	41.0	35.0	41.0
54-55	38.350391615901	40.0	37.0	41.0	34.5	41.0
56-57	38.19276700817943	40.0	37.0	41.0	34.0	41.0
58-59	38.02393636923031	39.5	36.5	41.0	34.0	41.0
60-61	37.79161525341027	39.0	36.0	41.0	34.0	41.0
62-63	37.624295378209105	39.0	35.0	41.0	34.0	41.0
64-65	37.35741803458673	39.0	35.0	40.5	33.5	41.0
66-67	37.1250226970273	38.0	35.0	40.0	33.0	41.0
68-69	36.867711657565046	37.0	35.0	40.0	33.0	41.0
70-71	36.434858332079784	37.0	35.0	39.5	33.0	41.0
72-73	36.18874177099708	36.0	35.0	39.0	33.0	41.0
74-75	35.83959176291258	36.0	35.0	39.0	32.5	40.0
76-77	34.05765693985353	34.5	33.0	36.5	30.0	39.0
78-79	35.03387710821442	35.0	34.0	37.0	31.5	39.0
80-81	34.97903491817103	35.0	34.0	37.0	32.0	39.0
82-83	34.727327004268666	35.0	34.0	36.0	32.0	38.0
84-85	34.50319035064774	35.0	34.0	36.0	32.0	37.0
86-87	34.3349936548429	35.0	34.0	35.5	32.0	37.0
88-89	34.18882185265275	35.0	34.0	35.0	31.5	36.5
90-91	34.132680232569946	35.0	34.0	35.0	32.0	36.0
92-93	34.02465375204847	35.0	34.0	35.0	31.5	36.0
94-95	33.88702366209612	35.0	34.0	35.0	31.0	36.0
96-97	33.91743496286694	35.0	34.0	35.0	31.0	35.5
98-99	33.952210943048016	35.0	34.0	35.0	31.0	35.0
100-101	32.733341558718834	34.0	32.0	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	6.0
19	19.0
20	24.0
21	79.0
22	241.0
23	816.0
24	2219.0
25	4874.0
26	9391.0
27	15822.0
28	24078.0
29	34714.0
30	48470.0
31	66222.0
32	91208.0
33	131084.0
34	219324.0
35	370123.0
36	776503.0
37	1654956.0
38	2150798.0
39	1100142.0
40	20608.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.233156141661894	20.064498343653852	12.772662604513755	23.929682910170502
2	15.478195901035594	21.15776946996191	40.4389012279576	22.925133401044892
3	18.374149784346972	26.740648204309732	30.216819403848177	24.668382607495122
4	12.362023844183998	17.473287726971197	39.197063014944234	30.967625413900574
5	12.888897682930404	38.49197594128767	35.516384712669655	13.102741663112273
6	29.83921886241603	37.920467549500984	18.891798543341224	13.348515044741763
7	26.61216476787276	32.80148557148219	23.412538719611028	17.173810941034017
8	25.436305542492605	35.53132135912176	21.298675949603993	17.733697148781644
9	26.649421085846754	15.513896587808897	21.224814117572848	36.611868208771504
10-11	24.932749502636796	26.269926607081022	28.553880920704927	20.243442969577252
12-13	25.62581922297958	24.0388818912269	28.681144822295053	21.65415406349847
14-15	22.733724671486762	25.163585289069484	26.66179489990885	25.44089513953491
16-17	21.98893051677375	28.03878559113489	27.261722924315684	22.710560967775674
18-19	21.711323123550315	27.583582364224174	29.20864932994115	21.49644518228436
20-21	22.943791922032652	26.693389945860886	28.77848860827611	21.584329523830352
22-23	22.718322672904538	26.727096221798814	28.514599308260586	22.03998179703606
24-25	22.26914597919035	26.78663215356011	28.887985087575967	22.056236779673576
26-27	22.281500392626803	27.068741324064117	28.546529067408997	22.103229215900082
28-29	22.032489374462205	27.54673560383972	28.414584086092	22.006190935606075
30-31	22.476556400973912	27.090218450928923	28.46022786638286	21.97299728171431
32-33	22.14413921906748	27.284239054425008	28.287811994866125	22.283809731641387
34-35	22.246443068364698	27.43316737459814	28.463367844207337	21.85702171282983
36-37	22.426386296144717	27.227769203295082	28.341690827570016	22.00415367299018
38-39	22.234844222336843	27.322304851131623	28.391511758264933	22.05133916826661
40-41	22.346191146381557	27.11869602809076	28.42865764045846	22.106455185069223
42-43	22.375426502799215	27.279059924382203	27.98362008561189	22.36189348720669
44-45	22.36327296129889	27.757061792105866	27.72310327269731	22.156561973897936
46-47	22.361028385068302	27.487534134663893	27.50836482347619	22.643072656791617
48-49	22.610109951717153	27.54836868645124	27.400741009360285	22.440780352471318
50-51	22.433791062924172	27.54317506315821	27.311104580176544	22.711929293741072
52-53	22.470786845276105	27.51994617732705	27.150819396120596	22.858447581276252
54-55	22.455147526087423	27.612085552448807	27.138253251401025	22.79451367006275
56-57	22.727279912656392	27.575036322912805	26.979756826178352	22.717926938252454
58-59	22.486129853213097	27.697905816383656	27.264562148562355	22.551402181840892
60-61	22.655976223423533	27.761036632048086	27.056080501564807	22.526906642963574
62-63	22.724522486330617	27.46261314597095	27.006581942062745	22.80628242563569
64-65	22.46162915021512	27.611186599174264	27.0753329350553	22.85185131555531
66-67	22.64981805951975	27.573002698629622	26.881823360958435	22.895355880892193
68-69	22.57298707399339	27.550196721899468	27.063535880173095	22.813280323934045
70-71	22.58245269599447	27.23825947065478	27.032175528338282	23.14711230501247
72-73	22.701965852191215	27.437441232642882	27.250751155712344	22.60984175945356
74-75	22.64061656043528	27.544218764044853	27.12506974344938	22.690094932070487
76-77	22.632259790091993	27.31342953474078	26.984541080195555	23.069769594971675
78-79	22.609812272298903	27.412203037725906	27.004676698036	22.973307991939187
80-81	22.500604070756253	27.500427528950727	27.13277390142076	22.866194498872257
82-83	22.918683359864332	27.149235494983202	27.42586620477878	22.506214940373685
84-85	23.11016121214694	27.27003972205887	27.027376292957577	22.59242277283661
86-87	22.551388885317674	27.46326179189465	27.175893872976115	22.809455449811562
88-89	22.878319201908813	27.075936735609098	27.14521801144886	22.900526051033225
90-91	22.96384876961867	27.015492987429734	27.19770007983068	22.822958163120912
92-93	23.09757320666637	27.083678300320557	27.077623797759315	22.741124695253752
94-95	22.593419045648545	27.09877686729828	27.41295644935451	22.89484763769866
96-97	22.680376167727122	27.14773936034689	27.304502517333646	22.867381954592343
98-99	22.866931879111785	27.777549185527235	27.289118537491202	22.06640039786978
100-101	27.154146514855803	34.60481229553911	28.017002126757955	10.224039062847122
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	843.0
1	1001.0
2	2169.5
3	3427.0
4	5276.5
5	9291.5
6	12178.5
7	13355.0
8	13383.5
9	12068.5
10	10252.5
11	8799.5
12	9878.5
13	14352.5
14	23814.5
15	35377.0
16	43655.5
17	47553.0
18	47015.5
19	43146.5
20	38077.5
21	33358.0
22	30950.5
23	32329.5
24	37147.5
25	45271.0
26	56987.5
27	69164.0
28	80918.0
29	96176.5
30	110526.0
31	123073.5
32	138510.5
33	154151.0
34	169853.0
35	187319.0
36	203582.5
37	220344.5
38	230977.5
39	234478.5
40	240274.0
41	244976.0
42	251995.5
43	265854.0
44	281023.5
45	298300.0
46	312661.0
47	322679.0
48	325910.0
49	320755.0
50	314793.0
51	309664.5
52	302993.5
53	295053.0
54	283453.0
55	264423.0
56	244995.5
57	217536.5
58	185603.0
59	159436.0
60	132182.5
61	105989.0
62	84307.0
63	65363.5
64	48837.0
65	35627.0
66	25989.5
67	19813.0
68	15235.0
69	11237.5
70	8347.0
71	5850.0
72	4094.5
73	2848.0
74	2014.0
75	1412.5
76	922.0
77	582.5
78	383.5
79	270.0
80	193.5
81	137.5
82	102.0
83	71.5
84	46.5
85	29.0
86	22.0
87	18.5
88	13.5
89	7.5
90	4.5
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0011009082046374123
3	6.843483434232562E-4
4	0.0
5	0.0
6	5.802083781197172E-4
7	0.0
8	0.0
9	1.487713790050557E-5
10-11	3.79367016462892E-4
12-13	4.983841196669366E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.0420416155694117E-4
28-29	7.449982123767894E-6
30-31	5.9747431177867576E-5
32-33	3.149607007785604E-4
34-35	2.2613599984863965E-5
36-37	7.974040776054913E-4
38-39	2.688430148823812E-4
40-41	1.1671270723605558E-4
42-43	0.0
44-45	0.0
46-47	0.0
48-49	8.273702005082039E-6
50-51	8.406522620901558E-6
52-53	0.0
54-55	8.131572300058477E-4
56-57	3.5128530022071256E-5
58-59	0.0
60-61	9.789576675137068E-4
62-63	0.0
64-65	0.0
66-67	9.487183573966257E-6
68-69	0.0
70-71	0.0
72-73	4.983449465978539E-5
74-75	1.012241543101498E-5
76-77	1.0287085818778993E-5
78-79	4.182436631118395E-5
80-81	0.0
82-83	0.0
84-85	2.1996317596471177E-5
86-87	5.254316756597466E-4
88-89	1.1370621543188918E-4
90-91	1.157973125528108E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	133.0
20-21	511.0
22-23	972.0
24-25	1820.0
26-27	4417.0
28-29	12687.0
30-31	26233.0
32-33	32136.0
34-35	43392.0
36-37	68403.0
38-39	85274.0
40-41	81191.0
42-43	89008.0
44-45	103937.0
46-47	103119.0
48-49	98350.0
50-51	87674.0
52-53	81104.0
54-55	86193.0
56-57	89041.0
58-59	87422.0
60-61	86410.0
62-63	80052.0
64-65	80702.0
66-67	84637.0
68-69	85527.0
70-71	85229.0
72-73	77770.0
74-75	78587.0
76-77	77878.0
78-79	80168.0
80-81	81141.0
82-83	76063.0
84-85	73634.0
86-87	74319.0
88-89	78255.0
90-91	81930.0
92-93	79745.0
94-95	81445.0
96-97	113996.0
98-99	472940.0
100-101	3508278.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.83425573921413
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.49756779967105	72.42132595616721
2	7.3946047393995755	12.102639506688593
3	2.0982782555298076	5.151331181251751
4	0.8601646114528014	2.8156372316580125
5	0.42966306690464	1.758057864938469
6	0.25139742085461175	1.2343752498237084
7	0.14256294900129565	0.8166572979255999
8	0.0870953097849622	0.570190388370294
9	0.05731856207591491	0.42215596807719974
>10	0.17599161902923996	2.056887952238176
>50	0.003246083777636282	0.17674101086968708
>100	0.001812808095334102	0.28502589068692064
>500	2.9677442313569865E-4	0.18897450130432733
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.487713790050557E-5	5.950855160202228E-5	0.0	0.0	0.0
3	1.487713790050557E-5	7.438568950252785E-5	0.0	0.0	0.0
4	1.487713790050557E-5	7.438568950252785E-5	0.0	0.0	0.0
5	4.463141370151671E-5	7.438568950252785E-5	0.0	0.0	0.0
6	4.463141370151671E-5	7.438568950252785E-5	0.0	0.0	0.0
7	1.487713790050557E-4	7.438568950252785E-5	0.0	0.0	0.0
8	1.487713790050557E-4	7.438568950252785E-5	0.0	0.0	0.0
9	1.487713790050557E-4	7.438568950252785E-5	0.0	0.0	0.0
10-11	1.487713790050557E-4	7.438568950252785E-5	0.0	0.0	0.0
12-13	1.7852565480606683E-4	7.438568950252785E-5	0.0	0.0	0.0
14-15	2.380342064080891E-4	7.438568950252785E-5	0.0	0.0	0.0
16-17	2.6034991325884745E-4	8.926282740303343E-5	0.0	7.438568950252785E-6	0.0
18-19	3.7192844751263924E-4	1.4133281005480292E-4	0.0	4.463141370151671E-5	0.0
20-21	4.3143699911466155E-4	1.487713790050557E-4	0.0	7.438568950252785E-5	0.0
22-23	4.6119127491567265E-4	1.6364851690556126E-4	0.0	1.1901710320404456E-4	0.0
24-25	5.50454102318706E-4	2.2315706850758354E-4	0.0	1.8596422375631962E-4	0.0
26-27	5.653312402192117E-4	2.2315706850758354E-4	0.0	3.1241989591061694E-4	0.0
28-29	6.992254813237618E-4	2.2315706850758354E-4	0.0	0.0014505209452992931	0.0
30-31	7.438568950252785E-4	2.2315706850758354E-4	0.0	0.004269738577445099	0.0
32-33	7.512954639755313E-4	2.2315706850758354E-4	0.0	0.009142001239860673	0.0
34-35	8.256811534780591E-4	2.6034991325884745E-4	0.0	0.015211873503266946	0.0
36-37	8.777511361298286E-4	2.6778848220910026E-4	0.0	0.02430180476047585	0.0
38-39	9.744525324831149E-4	2.975427580101114E-4	0.0	0.03861361142076221	0.0
40-41	9.893296703836203E-4	3.421741717116281E-4	0.0	0.05401888771673573	0.0
42-43	0.0010860310667369067	3.942441543633976E-4	0.0	0.0684869043249774	0.0
44-45	0.00117529389413994	4.3143699911466155E-4	0.0	0.08346074362183625	0.0
46-47	0.0013240652731449957	4.3143699911466155E-4	0.0	0.099490859709631	0.0
48-49	0.0013984509626475236	5.430155333684533E-4	0.0	0.11535732728052019	0.0
50-51	0.0013984509626475236	7.066640502740145E-4	0.0	0.1316775475573748	0.0
52-53	0.0013984509626475236	7.661726018760368E-4	0.0	0.1468299125090397	0.0
54-55	0.001413328100548029	7.736111708262896E-4	0.0	0.16208641742600818	0.0
56-57	0.0015025909279510626	8.256811534780591E-4	0.0	0.1741368991254177	0.0
58-59	0.0015323452037520737	8.703125671795759E-4	0.0	0.18399300298450263	0.0
60-61	0.0015323452037520737	9.298211187815981E-4	0.0	0.19337303843077142	0.0
62-63	0.0015472223416525793	9.521368256323564E-4	0.0	0.20258198679118433	0.0
64-65	0.0015472223416525793	9.670139635328621E-4	0.0	0.21117353392872631	0.0
66-67	0.001695993720657635	9.670139635328621E-4	0.0	0.21986178246262156	0.0
68-69	0.0017108708585581406	9.670139635328621E-4	0.0	0.22881781947872593	0.0
70-71	0.0017480637033094044	0.0010190839461846316	0.0	0.23702999959980497	0.0
72-73	0.0017852565480606683	0.0010860310667369067	0.0	0.2433602217764701	0.0
74-75	0.001800133685961174	0.0011157853425379177	0.0	0.249065604161314	0.0
76-77	0.001800133685961174	0.0011529781872891818	0.0	0.25383372685842603	0.0
78-79	0.001800133685961174	0.0011976096009906983	0.0	0.25833406107332896	0.0
80-81	0.0018893965133642073	0.001205048169940951	0.0	0.2603499132588475	0.0
82-83	0.0019265893581154713	0.001205048169940951	0.0	0.2611235244296738	0.0
84-85	0.001971220771816988	0.001205048169940951	0.0	0.26172604851464426	0.0
86-87	0.0020307293234190105	0.001205048169940951	0.0	0.2618525041867985	0.0
88-89	0.0020530450302697688	0.001205048169940951	0.0	0.26191201273840053	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10860	0.0	38.791416	1
GTATCAA	18535	0.0	33.565094	1
ATCAACG	21905	0.0	27.50759	3
CAACGCA	22010	0.0	27.321936	5
AACGCAG	23120	0.0	26.45964	6
TATCAAC	23425	0.0	26.38787	2
TCAACGC	23160	0.0	26.15493	4
ACGCAGA	26055	0.0	23.080763	7
TACATGG	26475	0.0	23.07642	2
CGCAGAG	26320	0.0	22.848375	8
GTACATG	27390	0.0	22.626257	1
ACATGGG	26660	0.0	22.197016	3
GCAGAGT	30445	0.0	19.700178	9
CATGGGG	18760	0.0	19.667341	4
GTACTAG	2745	0.0	18.765509	1
CGACGAG	1115	0.0	18.517004	24-25
ACGGTAC	735	0.0	18.471329	3
GGTTCAC	2525	0.0	17.712084	6
GAGTACT	16455	0.0	17.678337	12-13
GGCGTCG	1665	0.0	17.267698	8
>>END_MODULE