FastQCFastQC Report
Fri 27 May 2016
SRR2140097_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140097_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6681419
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83700.037.125351
CTTATAC74250.035.729981
GTATCAA136900.031.8194311
TATACAC93950.031.244253
ATCAACG155900.026.9694483
TTATACA103450.026.7132972
CAACGCA159450.026.3170555
TCAACGC161650.026.1361714
AACGCAG165500.025.7757246
TATCAAC172950.024.8555242
ACACATC113250.023.750266
ACGCAGA183800.022.7412827
CGCAGAG184200.022.69198
TACACAT131300.022.4182725
ACATCTC121250.022.351728
TACATGG195250.021.450572
GTACATG200800.021.1635191
CACATCT130750.020.6963847
ACATGGG197050.020.4024723
ATACACA163900.019.9075284
Produced by FastQC (version 0.11.5)