##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140097_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6681419
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72991381022504	34.0	31.0	34.0	31.0	34.0
2	32.941168934323684	34.0	31.0	34.0	31.0	34.0
3	33.05379201633665	34.0	33.0	34.0	31.0	34.0
4	36.43971348002572	37.0	37.0	37.0	35.0	37.0
5	36.29698646350423	37.0	37.0	37.0	35.0	37.0
6	36.31890022164453	37.0	37.0	37.0	35.0	37.0
7	36.3287957243813	37.0	37.0	37.0	35.0	37.0
8	36.3370435232396	37.0	37.0	37.0	35.0	37.0
9	38.25441706320169	39.0	39.0	39.0	37.0	39.0
10-11	38.15569956022815	39.0	38.5	39.0	37.0	39.0
12-13	38.14293767536507	39.0	39.0	39.0	37.0	39.0
14-15	39.63484889961249	41.0	39.5	41.0	37.0	41.0
16-17	39.61241339601662	41.0	39.0	41.0	37.0	41.0
18-19	39.657695992423164	41.0	40.0	41.0	37.5	41.0
20-21	39.677983868773126	41.0	40.0	41.0	37.5	41.0
22-23	39.62945980279328	41.0	40.0	41.0	37.0	41.0
24-25	39.61256467669799	41.0	39.5	41.0	37.0	41.0
26-27	39.49633047569887	41.0	39.0	41.0	37.0	41.0
28-29	39.42697166547802	40.5	39.0	41.0	37.0	41.0
30-31	39.36640940912288	40.0	39.0	41.0	36.5	41.0
32-33	39.28023854987386	40.0	39.0	41.0	36.0	41.0
34-35	39.24668733585371	40.0	39.0	41.0	36.0	41.0
36-37	39.21001492777195	40.0	39.0	41.0	36.0	41.0
38-39	39.13418846515816	40.0	39.0	41.0	35.0	41.0
40-41	39.09752772979605	40.0	39.0	41.0	35.0	41.0
42-43	39.10160308155584	40.0	39.0	41.0	35.0	41.0
44-45	39.046337943369416	40.0	39.0	41.0	35.0	41.0
46-47	38.948551424701094	40.0	38.5	41.0	35.0	41.0
48-49	38.946460087883764	40.0	38.5	41.0	35.0	41.0
50-51	38.832300175539224	40.0	38.0	41.0	35.0	41.0
52-53	38.68564648214432	40.0	38.0	41.0	35.0	41.0
54-55	38.48848849204819	40.0	38.0	41.0	34.5	41.0
56-57	38.35549629475072	40.0	37.0	41.0	34.0	41.0
58-59	38.21923847967222	40.0	37.0	41.0	34.0	41.0
60-61	38.00583957600212	40.0	36.5	41.0	34.0	41.0
62-63	37.872791052987765	39.0	36.0	41.0	34.0	41.0
64-65	37.64259365683884	39.0	36.0	41.0	33.5	41.0
66-67	37.43947862060013	39.0	35.0	40.5	33.0	41.0
68-69	37.20783580332824	38.5	35.0	40.0	33.0	41.0
70-71	36.78449269400845	37.0	35.0	40.0	33.0	41.0
72-73	36.52966263722094	37.0	35.0	39.5	33.0	41.0
74-75	36.1700916976688	36.5	35.0	39.0	32.5	41.0
76-77	34.34980472262901	34.5	33.0	36.5	30.0	39.0
78-79	35.34377642962575	35.0	34.0	37.0	32.0	39.0
80-81	35.25670153528381	35.0	35.0	37.0	32.0	39.0
82-83	34.96761986759458	35.0	34.5	37.0	32.0	39.0
84-85	34.702046516363566	35.0	34.0	36.0	32.0	37.5
86-87	34.4936934424217	35.0	34.0	36.0	32.0	37.0
88-89	34.31141136177234	35.0	34.0	36.0	32.0	37.0
90-91	34.22804156739661	35.0	34.0	35.0	32.0	36.0
92-93	34.09640701399238	35.0	34.0	35.0	31.5	36.0
94-95	33.94096089012632	35.0	34.0	35.0	31.0	36.0
96-97	33.95865166069136	35.0	34.0	35.0	31.0	36.0
98-99	33.98175506007311	35.0	34.0	35.0	31.0	35.5
100-101	32.78363980219855	34.5	32.0	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	13.0
20	22.0
21	63.0
22	322.0
23	853.0
24	2392.0
25	5229.0
26	9922.0
27	16217.0
28	25036.0
29	35843.0
30	49203.0
31	66112.0
32	90183.0
33	128343.0
34	206386.0
35	341632.0
36	698764.0
37	1545832.0
38	2243475.0
39	1189255.0
40	26320.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.71470162251462	20.581211865323816	13.139439391542426	24.56464712061914
2	14.552949239320956	21.308217985842045	41.48824792896866	22.650584845868334
3	17.881093743943055	26.83590413419022	30.784563849898696	24.498438271968027
4	11.750363208773464	18.24657307078032	39.67723024106107	30.325833479385146
5	12.513240076696283	38.40372830980964	36.37689838041889	12.706133233075189
6	29.03821443939309	38.89166526351238	19.14179341827094	12.928326878823595
7	25.74388763823972	32.81145816480002	23.748024184682926	17.696630012277332
8	24.274559042023856	36.75999065467979	21.888643714755805	17.076806588540546
9	25.470079995019017	16.12590804104998	21.342586661270605	37.061425302660396
10-11	23.90320777614946	26.79388742292133	29.440785990430605	19.8621188104986
12-13	24.77630780776039	24.24036402000721	29.35047507322951	21.632853099002887
14-15	21.82045460702285	25.805214431245815	26.72911248344102	25.645218478290317
16-17	21.573905183913777	28.88602705503127	27.3839733745182	22.15609438653675
18-19	21.378804412655455	28.197962139479653	28.94857364880125	21.47465979906364
20-21	22.253055071360084	27.410387916157404	28.744410049353515	21.592146963129004
22-23	21.899302927214727	27.76275096543735	28.426668299242095	21.91127780810583
24-25	21.817247499301985	27.743873729627445	28.75379418186772	21.68508458920285
26-27	21.9543102358705	27.96534062762627	28.52380868234904	21.556540454154195
28-29	21.35980280819686	28.008087645330498	28.610427499672557	22.021682046800084
30-31	22.021421074564028	27.66180006962041	28.8074438733329	21.509334982482667
32-33	21.60877957616104	28.121882188594682	28.54687798789279	21.722460247351485
34-35	21.53507065796391	28.24638996759153	28.897274167661347	21.321265206783213
36-37	21.967052285776298	27.9941383075508	28.44834111562799	21.59046829104491
38-39	21.60222128268081	27.831414127633618	28.728846786787553	21.837517802898013
40-41	21.727940038578943	27.713959186560995	28.79716997780474	21.760930797055327
42-43	21.95755249315034	27.67352207251547	28.622693530285837	21.74623190404835
44-45	21.74116091245591	28.24930697924792	28.33714987741487	21.672382230881304
46-47	21.999492292263454	27.762184101448945	27.91653014719666	22.321793459090944
48-49	21.989554131243985	27.7512298889491	28.1021681955622	22.157047784244714
50-51	21.701962037212912	27.94391807740057	28.17025874837099	22.183861137015523
52-53	21.77099846483541	28.03839598095147	28.24716199952474	21.94344355468838
54-55	21.76994333384405	28.127838198784772	27.933373385203016	22.168845082168165
56-57	22.23927239789044	28.23285467749721	27.556108358236013	21.971764566376333
58-59	21.785579071537263	28.508009521660664	28.07158558873643	21.634825818065636
60-61	22.08315415271586	28.785097205558476	27.41481086111471	21.71693778061096
62-63	21.872939731125488	28.81186489352401	27.366769316736423	21.948426058614082
64-65	21.803615423566786	28.627669502782755	27.60293466487735	21.965780408773114
66-67	21.95063496179602	28.445048620856944	27.501090626369624	22.10322579097741
68-69	21.998927525934985	28.27080232451821	27.68491371492725	22.045356434619553
70-71	21.95231208007986	27.939691652955457	27.738744935581	22.369251331383683
72-73	22.05942802830508	28.094145864942465	27.899890018043827	21.94653608870863
74-75	21.94804883004757	28.200180084272258	27.783864519475003	22.067906566205174
76-77	21.870467441825276	28.16443575927145	27.63982467606378	22.32527212283949
78-79	22.037650283291256	28.1345516705093	27.63949611182422	22.188301934375218
80-81	21.862645498686188	28.214768078520592	27.684697570551688	22.237888852241536
82-83	22.08119900407596	28.07807621938388	27.844187155814907	21.996537620725253
84-85	22.41523945528378	27.97495352778879	27.636879694278942	21.972927322648488
86-87	21.84055211178262	28.168755191630808	27.775187704352554	22.21550499223402
88-89	22.1267731550789	28.021318184975673	27.73642623294213	22.11548242700329
90-91	22.232060339872568	27.98491328480008	27.727047595087974	22.055978780239382
92-93	22.1947290481116	28.04885924163075	27.68645605613105	22.0699556541266
94-95	21.925180309506086	28.151368971821633	27.858806922919637	22.064643795752644
96-97	21.918660495356416	28.21745817407455	27.817882718688885	22.04599861188015
98-99	22.21117523847413	28.768850034035363	27.816778122345088	21.203196605145422
100-101	26.202310740185702	35.69355170585517	28.496031778887243	9.608105775071888
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	761.0
1	796.0
2	1520.0
3	2363.0
4	3526.0
5	6229.0
6	8289.0
7	9207.0
8	9239.5
9	8229.5
10	7034.0
11	6166.5
12	6821.5
13	9538.5
14	15823.0
15	23920.5
16	29985.0
17	33339.0
18	34020.5
19	32630.0
20	30428.0
21	28446.5
22	28552.0
23	32332.0
24	39149.5
25	49433.0
26	63000.5
27	77220.5
28	92724.5
29	110809.0
30	127158.0
31	140880.5
32	156551.5
33	173424.0
34	191135.5
35	209065.5
36	226000.0
37	241580.5
38	251097.0
39	256911.5
40	265009.0
41	270787.5
42	272949.5
43	277585.0
44	285041.0
45	296791.5
46	310438.0
47	321903.5
48	321308.5
49	311117.5
50	302592.5
51	290348.5
52	271760.5
53	253452.5
54	235466.0
55	213861.0
56	192589.0
57	168137.5
58	142471.5
59	121757.5
60	101622.5
61	81129.5
62	63023.0
63	48248.0
64	35772.0
65	25978.0
66	19333.0
67	14729.0
68	11332.5
69	8477.5
70	6452.0
71	4696.0
72	3331.5
73	2451.5
74	1767.0
75	1257.0
76	896.0
77	608.5
78	422.0
79	291.0
80	189.0
81	121.5
82	82.0
83	54.5
84	35.0
85	26.0
86	25.0
87	18.0
88	11.0
89	6.0
90	2.0
91	1.5
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0012871517263024516
3	5.687414604592228E-4
4	0.0
5	0.0
6	6.585427436896264E-4
7	0.0
8	0.0
9	4.49006416152018E-5
10-11	2.1701976780680868E-4
12-13	4.190726550752168E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.9900541141488674E-5
28-29	0.0
30-31	5.254934120392343E-5
32-33	3.0879111742015714E-4
34-35	7.562172019146815E-5
36-37	7.455245514547732E-4
38-39	2.3799839358761696E-4
40-41	7.757049820195463E-5
42-43	0.0
44-45	7.930205940311085E-6
46-47	0.0
48-49	3.2586303194695996E-5
50-51	0.0
52-53	0.0
54-55	7.098747721745654E-4
56-57	1.0281502394818946E-4
58-59	8.69145231299971E-6
60-61	8.290158364954996E-4
62-63	0.0
64-65	0.0
66-67	3.690001157737863E-5
68-69	0.0
70-71	0.0
72-73	7.708559410927307E-5
74-75	0.0
76-77	1.9777982260138814E-5
78-79	5.011695794475568E-5
80-81	0.0
82-83	0.0
84-85	4.182915550699656E-5
86-87	6.466307938516648E-4
88-89	9.677757556043625E-5
90-91	2.1838214210606427E-5
92-93	2.2191794805788508E-5
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	150.0
20-21	465.0
22-23	857.0
24-25	1592.0
26-27	3625.0
28-29	9434.0
30-31	20745.0
32-33	25051.0
34-35	34521.0
36-37	54935.0
38-39	68474.0
40-41	64845.0
42-43	71549.0
44-45	83244.0
46-47	83962.0
48-49	79879.0
50-51	72307.0
52-53	70361.0
54-55	78759.0
56-57	83340.0
58-59	82617.0
60-61	83508.0
62-63	82131.0
64-65	82311.0
66-67	86202.0
68-69	77255.0
70-71	73457.0
72-73	66568.0
74-75	66658.0
76-77	66899.0
78-79	70247.0
80-81	71589.0
82-83	66557.0
84-85	64299.0
86-87	66063.0
88-89	69771.0
90-91	72818.0
92-93	72635.0
94-95	76003.0
96-97	112984.0
98-99	492485.0
100-101	3770267.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.85280366411979
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.6674958317742	73.46350623539149
2	7.3667118656768515	12.207054637141315
3	2.126607656838812	5.285862199880398
4	0.836149149131681	2.7710920514771193
5	0.4053041695937073	1.679029339379827
6	0.215674686067716	1.0721551452053488
7	0.12620525231937502	0.7319521294258513
8	0.07535779949796116	0.49948839730917455
9	0.04716773595352372	0.35171812496145105
>10	0.13033399343387514	1.5305660663241945
>50	0.0018846115271738022	0.10726923036753076
>100	8.342232213637493E-4	0.1456047510944143
>500	2.4268887176084154E-4	0.12700369041935053
>1k	3.033609199834973E-5	0.027698001622520924
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.49668805384006E-5	0.0
6	0.0	0.0	0.0	1.49668805384006E-5	1.49668805384006E-5
7	0.0	0.0	0.0	5.98675221536024E-5	1.49668805384006E-5
8	0.0	0.0	0.0	5.98675221536024E-5	2.99337610768012E-5
9	0.0	0.0	0.0	1.49668805384006E-4	4.49006416152018E-5
10-11	0.0	0.0	0.0	1.796025664608072E-4	4.49006416152018E-5
12-13	0.0	1.49668805384006E-5	0.0	2.0953632753760838E-4	5.98675221536024E-5
14-15	0.0	1.49668805384006E-5	0.0	2.544369691528102E-4	9.72847234996039E-5
16-17	0.0	1.49668805384006E-5	0.0	2.918541704988117E-4	1.197350443072048E-4
18-19	0.0	1.49668805384006E-5	0.0	3.442382523832138E-4	1.197350443072048E-4
20-21	0.0	1.49668805384006E-5	0.0	3.666885731908147E-4	1.197350443072048E-4
22-23	0.0	1.49668805384006E-5	0.0	4.041057745368162E-4	1.49668805384006E-4
24-25	0.0	1.49668805384006E-5	0.0	4.7894017722881926E-4	1.870860067300075E-4
26-27	0.0	1.49668805384006E-5	0.0	6.73509624228027E-4	2.095363275376084E-4
28-29	0.0	1.49668805384006E-5	0.0	0.0011823835625336474	2.095363275376084E-4
30-31	0.0	1.49668805384006E-5	0.0	0.0027988066606809124	2.170197678068087E-4
32-33	0.0	1.49668805384006E-5	0.0	0.005462911396516219	2.24503208076009E-4
34-35	0.0	1.49668805384006E-5	0.0	0.008673307272003147	2.24503208076009E-4
36-37	0.0	1.49668805384006E-5	0.0	0.013462709044291341	2.24503208076009E-4
38-39	0.0	1.49668805384006E-5	0.0	0.02065429514299283	2.319866483452093E-4
40-41	0.0	1.49668805384006E-5	0.0	0.028294887657846334	2.394700886144096E-4
42-43	0.0	1.49668805384006E-5	0.0	0.035149718944433805	2.394700886144096E-4
44-45	0.0	1.49668805384006E-5	0.0	0.042610708892826504	2.394700886144096E-4
46-47	0.0	1.49668805384006E-5	0.0	0.05103706263594605	2.394700886144096E-4
48-49	0.0	1.49668805384006E-5	0.0	0.06019679352544721	2.394700886144096E-4
50-51	0.0	1.49668805384006E-5	0.0	0.06868301479072036	2.469535288836099E-4
52-53	0.0	1.49668805384006E-5	0.0	0.07682499780361028	2.99337610768012E-4
54-55	0.0	1.49668805384006E-5	0.0	0.08506426553999981	3.442382523832138E-4
56-57	0.0	1.49668805384006E-5	0.0	0.09315386447100533	3.442382523832138E-4
58-59	0.0	1.49668805384006E-5	0.0	0.10109379459662685	3.442382523832138E-4
60-61	0.0	1.49668805384006E-5	0.0	0.10867451958932675	3.442382523832138E-4
62-63	0.0	1.49668805384006E-5	0.0	0.11677908540087069	3.74172013460015E-4
64-65	0.0	1.49668805384006E-5	0.0	0.12385093645526497	3.74172013460015E-4
66-67	0.0	1.49668805384006E-5	0.0	0.13083298622642886	3.816554537292153E-4
68-69	0.0	1.49668805384006E-5	0.0	0.13825655897347555	3.966223342676159E-4
70-71	0.0	1.49668805384006E-5	0.0	0.14533589346813902	4.0410577453681623E-4
72-73	0.0	1.49668805384006E-5	0.0	0.15115800999757686	4.789401772288192E-4
74-75	0.0	1.49668805384006E-5	0.0	0.15576780920340424	4.8642361749801956E-4
76-77	0.0	1.49668805384006E-5	0.0	0.159943568873618	5.013904980364202E-4
78-79	0.0	1.49668805384006E-5	0.0	0.16389482533575578	5.088739383056204E-4
80-81	0.0	1.49668805384006E-5	0.0	0.16544389747148025	5.088739383056204E-4
82-83	0.0	1.49668805384006E-5	0.0	0.16605005613328547	5.537745799208222E-4
84-85	0.0	1.49668805384006E-5	0.0	0.1665439631910527	5.537745799208222E-4
86-87	0.0	1.49668805384006E-5	0.0	0.1668133670407439	5.537745799208222E-4
88-89	0.0	1.49668805384006E-5	0.0	0.1668807180031667	5.837083409976234E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8370	0.0	37.12535	1
CTTATAC	7425	0.0	35.72998	1
GTATCAA	13690	0.0	31.819431	1
TATACAC	9395	0.0	31.24425	3
ATCAACG	15590	0.0	26.969448	3
TTATACA	10345	0.0	26.713297	2
CAACGCA	15945	0.0	26.317055	5
TCAACGC	16165	0.0	26.136171	4
AACGCAG	16550	0.0	25.775724	6
TATCAAC	17295	0.0	24.855524	2
ACACATC	11325	0.0	23.75026	6
ACGCAGA	18380	0.0	22.741282	7
CGCAGAG	18420	0.0	22.6919	8
TACACAT	13130	0.0	22.418272	5
ACATCTC	12125	0.0	22.35172	8
TACATGG	19525	0.0	21.45057	2
GTACATG	20080	0.0	21.163519	1
CACATCT	13075	0.0	20.696384	7
ACATGGG	19705	0.0	20.402472	3
ATACACA	16390	0.0	19.907528	4
>>END_MODULE